NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHE231
Library SH
(Link to library)
Clone ID SHE231
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16507-1
Original site URL
Representative seq. ID SHE231P
(Link to Original site)
Representative DNA sequence 
>SHE231 (SHE231Q) /CSM/SH/SHE2-B/SHE231Q.Seq.d/
AAAATAAATGATATTAAAAAATCAAAACCAAAACTATCATCAACATTATTAAATGTTCAT
ATTGTTGCTCATACTCATGATGATGTTGGTTGGTTAAAAACAGTTGATGAATATTATTAT
GGATCAAATATGTCAATTGCATTTGCAGGTGTTCAATATACTTTGGATACAGCCATTACC
TGTTTATTGGCAAATCCAGAAAGAAAGTTTATTTATGTTGAAATTGCATTTTTCCAAAGA
TGGTGGGATGAACAAAGTACAACAATGCAAAATATTGTTAAGGGTTTGGTTGAAAGTGGT
CAATTGGAATTCATAAACGGTGGGTATTGTATGAATGATGAGGCTACTACCTACTATGAC
GATACTATTGACCAAATGACATTGGGCCATCAATTCCTTTGGGAGAATTTCGGAGTTATG
CCAAAGATAGGCTGGCACATAGATCCATTTGGCCATAGTGCAACACAAGCTCGTATCTTT
GGTCAATTAGGATTCGATGCATTCATTATCGGTCGTATGGATTATCAAGATATCGAAGCT
CGTTTAGAGXXXXXXXXXXACCAGTGGTAATTATGTACCTGTGAATGCTATTGCNTACAT
TCAAGATCNTAATCAATCATTACAATTCACCATTGTCACTGATAGAAGTCGTGGATGTGC
ATCGTTAAGAGATGGTCAATTGGATATGATGATGCATCGTCGTACTTTGAAAGATGATGG
TCGTGGTGTTGGTCAACCAATGAATGAGTCAACTCAAATCGTTACAACTAGTAAATTGAT
TTTCCATGATATTTCAAGTTATGCTCAATCTCATTATCGTCCTGCTGCACTCTCTTTATC
ACATCCATTACTCCCAATGTTTACAACCACTCAACAATCTTCAAACGATTGGAATTCTCA
ATATCAAGGTGTTTACTCACCATTGACTTCAGCCTCACCTTTACCAAATGGTTTAAAGAT
TCAAACTCTACAATGGTTAGATAATCAAGATAATACCATACTTTTACGTATTGAAAATAT
TTATCAAATTGATGGTCAAGATTCACAAGATCCACAAACTATCACTTTAGATCTTTCAAC
TATCTTTTCAACAATTACAATCACTTCTGCAACTGAAATGAATTTAACTGGTGTTCAAAA
ATTATCAAATTTATCAAGATTAAAATGGAAAACTGTTGAGGGTAAAAACTAGGATCATAA
ATCATCATCATCAACTAAAGAAGATTCTTCAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
KINDIKKSKPKLSSTLLNVHIVAHTHDDVGWLKTVDEYYYGSNMSIAFAGVQYTLDTAIT
CLLANPERKFIYVEIAFFQRWWDEQSTTMQNIVKGLVESGQLEFINGGYCMNDEATTYYD
DTIDQMTLGHQFLWENFGVMPKIGWHIDPFGHSATQARIFGQLGFDAFIIGRMDYQDIEA
RLE---

---TSGNYVPVNAIAYIQDXNQSLQFTIVTDRSRGCASLRDGQLDMMMHRRTLKDDGRGV
GQPMNESTQIVTTSKLIFHDISSYAQSHYRPAALSLSHPLLPMFTTTQQSSNDWNSQYQG
VYSPLTSASPLPNGLKIQTLQWLDNQDNTILLRIENIYQIDGQDSQDPQTITLDLSTIFS
TITITSATEMNLTGVQKLSNLSRLKWKTVEGKN*dhksssstkedss
Translated Amino Acid sequence (All Frames) 
Frame A:
KINDIKKSKPKLSSTLLNVHIVAHTHDDVGWLKTVDEYYYGSNMSIAFAGVQYTLDTAIT
CLLANPERKFIYVEIAFFQRWWDEQSTTMQNIVKGLVESGQLEFINGGYCMNDEATTYYD
DTIDQMTLGHQFLWENFGVMPKIGWHIDPFGHSATQARIFGQLGFDAFIIGRMDYQDIEA
RLE---

---TSGNYVPVNAIAYIQDXNQSLQFTIVTDRSRGCASLRDGQLDMMMHRRTLKDDGRGV
GQPMNESTQIVTTSKLIFHDISSYAQSHYRPAALSLSHPLLPMFTTTQQSSNDWNSQYQG
VYSPLTSASPLPNGLKIQTLQWLDNQDNTILLRIENIYQIDGQDSQDPQTITLDLSTIFS
TITITSATEMNLTGVQKLSNLSRLKWKTVEGKN*dhksssstkedss

Frame B:
k*milknqnqnyhqhy*mfilllilmmmlvg*kqlmniimdqicqlhlqvfnilwiqplp
vywqiqkeslfmlklhfskdggmnkvqqckillrvwlkvvnwns*tvgiv*mmrllptmt
illtk*hwainsfgriselcqr*agt*ihlaivqhklvslvn*dsmhslsvvwiikiskl
v*---

---pvvimyl*mllxtfkixinhynsplslievvdvhr*emvnwi**civvl*kmmvvvl
vnq*msqlkslqlvn*fsmifqvmlnliivllhslyhihysqclqplnnlqtigilnikv
fthh*lqphlyqmv*rfklyng*iikiipyfyvlkifiklmvkihkihklsl*ifqlsfq
qlqsllqlk*i*lvfknyqiyqd*ngkllrvktriinhhhqlkkilq

Frame C:
nk*y*kiktktiiniikcsyccsys**cwlvkns**illwikyvncicrcsiyfgyshyl
figksrkkvylc*ncifpkmvg*tkynnakyc*gfg*kwsigihkrwvlye**gyyll*r
yy*pndigpsiplgefrsyakdrlahrsiwp*cntssylwsirircihyrsyglsryrss
fr---

---qw*lctcecycxhsrs*siitihhch**kswmcivkrwsigyddassyfer*wswcw
stne*vnsnryn**idfp*yfklcsislsscctlfitsitpnvynhstifkrlefsisrc
lltidfsltftkwfkdsnstmvr*sr*yhtfty*kylsn*wsrftrstnyhfrsfnylfn
nynhfcn*nefnwcskiikfikikmenc*g*klgs*iiiin*rrffk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHE231 (SHE231Q) /CSM/SH/SHE2-B/SHE231Q.Seq.d/                       2373   0.0
SHE879 (SHE879Q) /CSM/SH/SHE8-D/SHE879Q.Seq.d/                       1324   0.0
VHL713 (VHL713Q) /CSM/VH/VHL7-A/VHL713Q.Seq.d/                       1318   0.0
VHL627 (VHL627Q) /CSM/VH/VHL6-B/VHL627Q.Seq.d/                       1316   0.0
VHD387 (VHD387Q) /CSM/VH/VHD3-D/VHD387Q.Seq.d/                       1316   0.0
SHK115 (SHK115Q) /CSM/SH/SHK1-A/SHK115Q.Seq.d/                       1316   0.0
VHL145 (VHL145Q) /CSM/VH/VHL1-B/VHL145Q.Seq.d/                       1312   0.0
VHQ387 (VHQ387Q) /CSM/VH/VHQ3-D/VHQ387Q.Seq.d/                       1308   0.0
VHQ167 (VHQ167Q) /CSM/VH/VHQ1-C/VHQ167Q.Seq.d/                       1308   0.0
VHP841 (VHP841Q) /CSM/VH/VHP8-B/VHP841Q.Seq.d/                       1308   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M82822|M82822.1 Dictyostelium discoideum alpha-mannosidase (manA) gene, complete cds.  1277   0.0    1
CK149800|CK149800.1 CsmgEST00336 Culicoides sonorensis female serum-fed midgut cDNA Library Culicoides sonorensis cDNA, mRNA sequence.   117   9e-24  3
DN814237|A Chain A, The Structure Of The Bovine Lysosomal A-Mannosidase Suggests A Novel Mechanism For Low Ph Activation, mRNA sequence.    52   5e-15  4
DN816506|A Chain A, The Structure Of The Bovine Lysosomal A-Mannosidase Suggests A Novel Mechanism For Low Ph Activation, mRNA sequence.    52   6e-15  4
CX633815|CX633815.1 taj44a03.y3 Hydra EST UCI 5 Hydra magnipapillata cDNA 5' similar to TR:Q93093 Q93093 TRUNCATED LYSOSOMAL ACID ALPHA-MANNOSIDASE ;, mRNA sequence.    52   8e-15  4
DN816232| truncated lysosomal acid alpha-mannosidase [Homo sapiens], mRNA sequence.    52   9e-15  4
DN811862| lysosomal alpha-mannosidase [Cavia porcellus], mRNA sequence.    52   9e-15  4
DT613126|A Chain A, The Structure Of The Bovine Lysosomal A-Mannosidase Suggests A Novel Mechanism For Low Ph Activation, mRNA sequence.    52   1e-14  4
DN815247| lysosomal alpha-mannosidase [Cavia porcellus], mRNA sequence.    52   1e-14  4
CV682907|CV682907.1 service43_H06_sjs2-16-2B11-T3 SJS Schistosoma japonicum cDNA, mRNA sequence.    74   2e-14  3
dna update 2006. 2.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P34098) RecName: Full=Lysosomal alpha-mannosidase;          Sho...   358   2e-97
M82822_1(M82822|pid:none) Dictyostelium discoideum alpha-mannosi...   351   3e-95
(Q29451) RecName: Full=Lysosomal alpha-mannosidase;          Sho...   220   7e-56
AK068415_1(AK068415|pid:none) Oryza sativa Japonica Group cDNA c...   220   9e-56
AK291572_1(AK291572|pid:none) Homo sapiens cDNA FLJ76867 complet...   219   2e-55
AY099704_1(AY099704|pid:none) Arabidopsis thaliana alpha-mannosi...   219   2e-55
AB005230_20(AB005230|pid:none) Arabidopsis thaliana genomic DNA,...   219   2e-55
U05572_1(U05572|pid:none) Human lysosomal alpha-mannosidase (MAN...   219   2e-55
AB209921_1(AB209921|pid:none) Homo sapiens mRNA for mannosidase,...   219   2e-55
JC2200(JC2200)alpha-mannosidase (EC 3.2.1.24) precursor - human       217   6e-55
protein update 2009. 4.17
PSORT 

 psg: 0.84  gvh: 0.43  alm: 0.41  top: 0.57  tms: 0.00  mit: 0.25  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 32.0 %: nuclear
	 16.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for SHE231 is cyt
5' end seq. ID SHE231F
5' end seq. 
>SHE231F.Seq
AAAATAAATGATATTAAAAAATCAAAACCAAAACTATCATCAACATTATTAAATGTTCAT
ATTGTTGCTCATACTCATGATGATGTTGGTTGGTTAAAAACAGTTGATGAATATTATTAT
GGATCAAATATGTCAATTGCATTTGCAGGTGTTCAATATACTTTGGATACAGCCATTACC
TGTTTATTGGCAAATCCAGAAAGAAAGTTTATTTATGTTGAAATTGCATTTTTCCAAAGA
TGGTGGGATGAACAAAGTACAACAATGCAAAATATTGTTAAGGGTTTGGTTGAAAGTGGT
CAATTGGAATTCATAAACGGTGGGTATTGTATGAATGATGAGGCTACTACCTACTATGAC
GATACTATTGACCAAATGACATTGGGCCATCAATTCCTTTGGGAGAATTTCGGAGTTATG
CCAAAGATAGGCTGGCACATAGATCCATTTGGCCATAGTGCAACACAAGCTCGTATCTTT
GGTCAATTAGGATTCGATGCATTCATTATCGGTCGTATGGATTATCAAGATATCGAAGCT
CGTTTAGAGNNNNNNNNNN
Length of 5' end seq. 559
3' end seq. ID SHE231Z
3' end seq. 
>SHE231Z.Seq
NNNNNNNNNNACCAGTGGTAATTATGTACCTGTGAATGCTATTGCNTACATTCAAGATCN
TAATCAATCATTACAATTCACCATTGTCACTGATAGAAGTCGTGGATGTGCATCGTTAAG
AGATGGTCAATTGGATATGATGATGCATCGTCGTACTTTGAAAGATGATGGTCGTGGTGT
TGGTCAACCAATGAATGAGTCAACTCAAATCGTTACAACTAGTAAATTGATTTTCCATGA
TATTTCAAGTTATGCTCAATCTCATTATCGTCCTGCTGCACTCTCTTTATCACATCCATT
ACTCCCAATGTTTACAACCACTCAACAATCTTCAAACGATTGGAATTCTCAATATCAAGG
TGTTTACTCACCATTGACTTCAGCCTCACCTTTACCAAATGGTTTAAAGATTCAAACTCT
ACAATGGTTAGATAATCAAGATAATACCATACTTTTACGTATTGAAAATATTTATCAAAT
TGATGGTCAAGATTCACAAGATCCACAAACTATCACTTTAGATCTTTCAACTATCTTTTC
AACAATTACAATCACTTCTGCAACTGAAATGAATTTAACTGGTGTTCAAAAATTATCAAA
TTTATCAAGATTAAAATGGAAAACTGTTGAGGGTAAAAACTAGGATCATAAATCATCATC
ATCAACTAAAGAAGATTCTTCAAA
Length of 3' end seq. 684
Connected seq. ID SHE231P
Connected seq. 
>SHE231P.Seq
AAAATAAATGATATTAAAAAATCAAAACCAAAACTATCATCAACATTATTAAATGTTCAT
ATTGTTGCTCATACTCATGATGATGTTGGTTGGTTAAAAACAGTTGATGAATATTATTAT
GGATCAAATATGTCAATTGCATTTGCAGGTGTTCAATATACTTTGGATACAGCCATTACC
TGTTTATTGGCAAATCCAGAAAGAAAGTTTATTTATGTTGAAATTGCATTTTTCCAAAGA
TGGTGGGATGAACAAAGTACAACAATGCAAAATATTGTTAAGGGTTTGGTTGAAAGTGGT
CAATTGGAATTCATAAACGGTGGGTATTGTATGAATGATGAGGCTACTACCTACTATGAC
GATACTATTGACCAAATGACATTGGGCCATCAATTCCTTTGGGAGAATTTCGGAGTTATG
CCAAAGATAGGCTGGCACATAGATCCATTTGGCCATAGTGCAACACAAGCTCGTATCTTT
GGTCAATTAGGATTCGATGCATTCATTATCGGTCGTATGGATTATCAAGATATCGAAGCT
CGTTTAGAG----------ACCAGTGGTAATTATGTACCTGTGAATGCTATTGCNTACAT
TCAAGATCNTAATCAATCATTACAATTCACCATTGTCACTGATAGAAGTCGTGGATGTGC
ATCGTTAAGAGATGGTCAATTGGATATGATGATGCATCGTCGTACTTTGAAAGATGATGG
TCGTGGTGTTGGTCAACCAATGAATGAGTCAACTCAAATCGTTACAACTAGTAAATTGAT
TTTCCATGATATTTCAAGTTATGCTCAATCTCATTATCGTCCTGCTGCACTCTCTTTATC
ACATCCATTACTCCCAATGTTTACAACCACTCAACAATCTTCAAACGATTGGAATTCTCA
ATATCAAGGTGTTTACTCACCATTGACTTCAGCCTCACCTTTACCAAATGGTTTAAAGAT
TCAAACTCTACAATGGTTAGATAATCAAGATAATACCATACTTTTACGTATTGAAAATAT
TTATCAAATTGATGGTCAAGATTCACAAGATCCACAAACTATCACTTTAGATCTTTCAAC
TATCTTTTCAACAATTACAATCACTTCTGCAACTGAAATGAATTTAACTGGTGTTCAAAA
ATTATCAAATTTATCAAGATTAAAATGGAAAACTGTTGAGGGTAAAAACTAGGATCATAA
ATCATCATCATCAACTAAAGAAGATTCTTCAAA
Length of connected seq. 1223
Full length Seq ID -
Full length Seq. -
Length of full length seq. -