NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHE223
Library SH
(Link to library)
Clone ID SHE223
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16101-1
Original site URL
Representative seq. ID SHE223P
(Link to Original site)
Representative DNA sequence 
>SHE223 (SHE223Q) /CSM/SH/SHE2-A/SHE223Q.Seq.d/
GTTATCAATCAATTAAAGAGAGACTACAACACCCTTCAAGAGGAACTCAACTCTGCCAAA
GAAGAGAACCAAAAGAGTATTCAAGATCAAGAGGCACTCAAACAAGCTGAAAAAGATTAT
GCCCTTAAAATCCGTGCTTTCAAAAAGAGTTTACTCGATGAAAAGAACGCCAGAAAATCT
GATATTGAAAAAATCAACTCTTTGGAGGACGAGTTAAAGGATGCTCAAGATAGTTTGACT
CAAGCAGAGAAAAGCAATGAAGAGTCCTTAAAGAGACTTAAATTGGATATGGTTAGAATT
AAAAAGAGAAACGATGATAAGGTCTCTCAATTAACTTTGGAGATTACAACACTCAAAGAT
ACCTTGGAAACTGAACGTTCAGCTTTATCCTCTGTTCAAACTTTGGTTGAAAATACTCGT
TTAGAGACTGAATCCAAATTCAATCTTTTATTAATCAAAAAGGAGGAAGCTATCGCTGCT
GCTGAATTATCACTTAGTGAGGAACAATCGAAATATAATAAACTCGTACAATCAAATAAC
CAACAAATCGATCAATTAXXXXXXXXXXAACTATTGATGATGATGAAACAACTGCACCAG
TAATTCCATCAATTGATAATTCACCAAGACAAACCACTACTGAAGAAGAAGAATCATCAA
CTACTGCCGCTACCACTTCCACAACTACAACTTCATCAACACCAGTTAAACCAGATGAAG
CTGATACCACAAAGAAAACACCAAAGAAGACATCATTCTTTGATTTTGATGATAGTCCAT
TCTCAGCTGAAACTGAAACCGAAACTAAATCAACAGCTGCATCATCTGATCCATTTGCCG
ATACAACTTCATCAACTCCAACTTCAACTAAAAAGAAAGATTTTTTTAGTACTGATGACG
ATAGTTTATTTGGAAACTCTAGTGACATTTTCGATAAACCTTCAACTACCACCAAGAAAG
ATCCATTCGATACAGATTCAACTGATGATCTTTTTGGTTCAATTAAAACAAACAAAGAAT
CAACATCAACAGCATCATCAACTTCAAATGGTGGTCTCTTTGATTCAGATTCATTATTTG
GTGGAATCAGCGTTGCAAAATCAAATAATAATACTCCATCAAGACAAAAACAAGATTTCT
CTTCATTATTTGGTTCNATCCAACCATTTCACCATTAACNAAACGAACCAGAAGAAAAAG
AAAAGTTTACCACTTTAA
sequence update 2002.10.25
Translated Amino Acid sequence 
VINQLKRDYNTLQEELNSAKEENQKSIQDQEALKQAEKDYALKIRAFKKSLLDEKNARKS
DIEKINSLEDELKDAQDSLTQAEKSNEESLKRLKLDMVRIKKRNDDKVSQLTLEITTLKD
TLETERSALSSVQTLVENTRLETESKFNLLLIKKEEAIAAAELSLSEEQSKYNKLVQSNN
QQIDQL---

---TIDDDETTAPVIPSIDNSPRQTTTEEEESSTTAATTSTTTTSSTPVKPDEADTTKKT
PKKTSFFDFDDSPFSAETETETKSTAASSDPFADTTSSTPTSTKKKDFFSTDDDSLFGNS
SDIFDKPSTTTKKDPFDTDSTDDLFGSIKTNKESTSTASSTSNGGLFDSDSLFGGISVAK
SNNNTPSRQKQDFSSLFGSIQPFHH*xnepeekekfttl
Translated Amino Acid sequence (All Frames) 
Frame A:
VINQLKRDYNTLQEELNSAKEENQKSIQDQEALKQAEKDYALKIRAFKKSLLDEKNARKS
DIEKINSLEDELKDAQDSLTQAEKSNEESLKRLKLDMVRIKKRNDDKVSQLTLEITTLKD
TLETERSALSSVQTLVENTRLETESKFNLLLIKKEEAIAAAELSLSEEQSKYNKLVQSNN
QQIDQL---

---ny****nnctsnsin**ftktnhy*rrriinycryhfhnynfints*tr*s*yhken
tkediil*f***sils*n*nrn*inscii*sicrynfinsnfn*kerff*y**r*fiwkl
**hfr*tfnyhqersiryrfn**sfwfn*nkqrininsiinfkwwsl*frfiiwwnqrck
ik**ysiktktrflfiiwfxptispltkrtrrkrkvyhf

Frame B:
lsin*retttpfkrnstlpkkrtkrvfkikrhsnklkkimplksvlskrvysmkrtpenl
ilkkstlwrts*rmlkiv*lkqrkamksp*rdlnwiwlelkretmirsln*lwrlqhski
pwklnvqlyplfklwlkilv*rlnpnsify*skrrklslllnyhlvrnnrniinsynqit
nksin---

---TIDDDETTAPVIPSIDNSPRQTTTEEEESSTTAATTSTTTTSSTPVKPDEADTTKKT
PKKTSFFDFDDSPFSAETETETKSTAASSDPFADTTSSTPTSTKKKDFFSTDDDSLFGNS
SDIFDKPSTTTKKDPFDTDSTDDLFGSIKTNKESTSTASSTSNGGLFDSDSLFGGISVAK
SNNNTPSRQKQDFSSLFGSIQPFHH*xnepeekekfttl

Frame C:
yqsikerlqhpsrgtqlcqrrepkeysrsrgtqts*krlcp*npcfqkeftr*kerqki*
y*knqlfggrvkgcsr*fdssrekq*rvlket*igyg*n*kekr**glsinfgdyntqry
lgn*tfsfilcsnfg*kysfrd*iqiqsfinqkggsyrcc*iit**gtiei**trtik*p
tnrsi---

---llmmmkqlhq*fhqliihqdkpllkkknhqllplplpqlqlhqhqlnqmklipqrkh
qrrhhslilmivhsqlklkpklnqqlhhlihlpiqlhqlqlqlkrkiflvlmtivyletl
vtfsinlqlpprkihsiqiqlmiflvqlkqtknqhqqhhqlqmvvsliqihylvesalqn
qiiilhqdknkislhylvxsnhftinxtnqkkkkslpl*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHE223 (SHE223Q) /CSM/SH/SHE2-A/SHE223Q.Seq.d/                       2302   0.0
VHH394 (VHH394Q) /CSM/VH/VHH3-D/VHH394Q.Seq.d/                       1055   0.0
SSH494 (SSH494Q) /CSM/SS/SSH4-D/SSH494Q.Seq.d/                        930   0.0
SLC359 (SLC359Q) /CSM/SL/SLC3-C/SLC359Q.Seq.d/                        831   0.0
VHP364 (VHP364Q) /CSM/VH/VHP3-C/VHP364Q.Seq.d/                        787   0.0
SLC827 (SLC827Q) /CSM/SL/SLC8-B/SLC827Q.Seq.d/                        781   0.0
SFJ117 (SFJ117Q) /CSM/SF/SFJ1-A/SFJ117Q.Seq.d/                        781   0.0
CHR294 (CHR294Q) /CSM/CH/CHR2-D/CHR294Q.Seq.d/                        490   e-137
CFC523 (CFC523Q) /CSM/CF/CFC5-A/CFC523Q.Seq.d/                         42   0.020
AFD432 (AFD432Q) /CSM/AF/AFD4-B/AFD432Q.Seq.d/                         42   0.020
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.    40   0.006  10
U51923|U51923.3 Dictyostelium discoideum STE20-telated kinase DPAK1 (dpak1) mRNA, complete cds.    42   0.009  4
AF131221|AF131221.1 Dictyostelium discoideum p21-activated protein kinase PakA (pakA) mRNA, complete cds.    42   0.009  4
AC121100|AC121100.3 Mus musculus clone RP24-502M21, WORKING DRAFT SEQUENCE, 5 unordered pieces.    52   0.029  1
AC091276|AC091276.7 Mus musculus chromosome 15, clone RP23-167B4, complete sequence.    52   0.029  1
AC124182|AC124182.4 Mus musculus BAC clone RP23-207J3 from chromosome 15, complete sequence.    52   0.029  1
AC116960|AC116960.2 Dictyostelium discoideum chromosome 2 map complement(1004496-821614) strain AX4, complete sequence.    38   0.26   9
AW586032|AW586032.1 EST317655 MHAM Medicago truncatula/Glomus versiforme mixed EST library cDNA clone pMHAM-51I16, mRNA sequence.    40   0.27   2
AW736347|AW736347.1 EST332266 KV3 Medicago truncatula cDNA clone pKV3-12C8, mRNA sequence.    40   0.33   2
AW684621|AW684621.1 NF019A09NR1F1000 Nodulated root Medicago truncatula cDNA clone NF019A09NR 5', mRNA sequence.    40   0.36   2
dna update 2006. 2.10
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54G21) RecName: Full=FK506-binding protein 5;          EC=5.2....   306   9e-82
(Q54G05) RecName: Full=Putative leucine-rich repeat-containing p...    55   4e-06
EU930270_1(EU930270|pid:none) Simulium vittatum clone SV-34 hypo...    53   2e-05
EU930276_1(EU930276|pid:none) Simulium vittatum clone SV-38 hypo...    52   3e-05
AB289560_1(AB289560|pid:none) Mizuhopecten yessoensis mRNA for n...    52   5e-05
BC070829_1(BC070829|pid:none) Xenopus laevis hypothetical protei...    52   5e-05
U59295_1(U59295|pid:none) Placopecten magellanicus myosin heavy ...    51   7e-05
U59294_1(U59294|pid:none) Placopecten magellanicus myosin heavy ...    51   7e-05
AY823086_1(AY823086|pid:none) Borrelia hermsii isolate RUM P-553...    51   7e-05
AY445042_1(AY445042|pid:none) Streptococcus pyogenes SpyM6 mefA ...    51   7e-05
protein update 2009. 4.17
PSORT 

 psg: 0.56  gvh: 0.22  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.03  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: nuclear
	 20.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for SHE223 is nuc
5' end seq. ID SHE223F
5' end seq. 
>SHE223F.Seq
GTTATCAATCAATTAAAGAGAGACTACAACACCCTTCAAGAGGAACTCAACTCTGCCAAA
GAAGAGAACCAAAAGAGTATTCAAGATCAAGAGGCACTCAAACAAGCTGAAAAAGATTAT
GCCCTTAAAATCCGTGCTTTCAAAAAGAGTTTACTCGATGAAAAGAACGCCAGAAAATCT
GATATTGAAAAAATCAACTCTTTGGAGGACGAGTTAAAGGATGCTCAAGATAGTTTGACT
CAAGCAGAGAAAAGCAATGAAGAGTCCTTAAAGAGACTTAAATTGGATATGGTTAGAATT
AAAAAGAGAAACGATGATAAGGTCTCTCAATTAACTTTGGAGATTACAACACTCAAAGAT
ACCTTGGAAACTGAACGTTCAGCTTTATCCTCTGTTCAAACTTTGGTTGAAAATACTCGT
TTAGAGACTGAATCCAAATTCAATCTTTTATTAATCAAAAAGGAGGAAGCTATCGCTGCT
GCTGAATTATCACTTAGTGAGGAACAATCGAAATATAATAAACTCGTACAATCAAATAAC
CAACAAATCGATCAATTANNNNNNNNNN
Length of 5' end seq. 568
3' end seq. ID SHE223Z
3' end seq. 
>SHE223Z.Seq
NNNNNNNNNNAACTATTGATGATGATGAAACAACTGCACCAGTAATTCCATCAATTGATA
ATTCACCAAGACAAACCACTACTGAAGAAGAAGAATCATCAACTACTGCCGCTACCACTT
CCACAACTACAACTTCATCAACACCAGTTAAACCAGATGAAGCTGATACCACAAAGAAAA
CACCAAAGAAGACATCATTCTTTGATTTTGATGATAGTCCATTCTCAGCTGAAACTGAAA
CCGAAACTAAATCAACAGCTGCATCATCTGATCCATTTGCCGATACAACTTCATCAACTC
CAACTTCAACTAAAAAGAAAGATTTTTTTAGTACTGATGACGATAGTTTATTTGGAAACT
CTAGTGACATTTTCGATAAACCTTCAACTACCACCAAGAAAGATCCATTCGATACAGATT
CAACTGATGATCTTTTTGGTTCAATTAAAACAAACAAAGAATCAACATCAACAGCATCAT
CAACTTCAAATGGTGGTCTCTTTGATTCAGATTCATTATTTGGTGGAATCAGCGTTGCAA
AATCAAATAATAATACTCCATCAAGACAAAAACAAGATTTCTCTTCATTATTTGGTTCNA
TCCAACCATTTCACCATTAACNAAACGAACCAGAAGAAAAAGAAAAGTTTACCACTTTAA
Length of 3' end seq. 660
Connected seq. ID SHE223P
Connected seq. 
>SHE223P.Seq
GTTATCAATCAATTAAAGAGAGACTACAACACCCTTCAAGAGGAACTCAACTCTGCCAAA
GAAGAGAACCAAAAGAGTATTCAAGATCAAGAGGCACTCAAACAAGCTGAAAAAGATTAT
GCCCTTAAAATCCGTGCTTTCAAAAAGAGTTTACTCGATGAAAAGAACGCCAGAAAATCT
GATATTGAAAAAATCAACTCTTTGGAGGACGAGTTAAAGGATGCTCAAGATAGTTTGACT
CAAGCAGAGAAAAGCAATGAAGAGTCCTTAAAGAGACTTAAATTGGATATGGTTAGAATT
AAAAAGAGAAACGATGATAAGGTCTCTCAATTAACTTTGGAGATTACAACACTCAAAGAT
ACCTTGGAAACTGAACGTTCAGCTTTATCCTCTGTTCAAACTTTGGTTGAAAATACTCGT
TTAGAGACTGAATCCAAATTCAATCTTTTATTAATCAAAAAGGAGGAAGCTATCGCTGCT
GCTGAATTATCACTTAGTGAGGAACAATCGAAATATAATAAACTCGTACAATCAAATAAC
CAACAAATCGATCAATTA----------AACTATTGATGATGATGAAACAACTGCACCAG
TAATTCCATCAATTGATAATTCACCAAGACAAACCACTACTGAAGAAGAAGAATCATCAA
CTACTGCCGCTACCACTTCCACAACTACAACTTCATCAACACCAGTTAAACCAGATGAAG
CTGATACCACAAAGAAAACACCAAAGAAGACATCATTCTTTGATTTTGATGATAGTCCAT
TCTCAGCTGAAACTGAAACCGAAACTAAATCAACAGCTGCATCATCTGATCCATTTGCCG
ATACAACTTCATCAACTCCAACTTCAACTAAAAAGAAAGATTTTTTTAGTACTGATGACG
ATAGTTTATTTGGAAACTCTAGTGACATTTTCGATAAACCTTCAACTACCACCAAGAAAG
ATCCATTCGATACAGATTCAACTGATGATCTTTTTGGTTCAATTAAAACAAACAAAGAAT
CAACATCAACAGCATCATCAACTTCAAATGGTGGTCTCTTTGATTCAGATTCATTATTTG
GTGGAATCAGCGTTGCAAAATCAAATAATAATACTCCATCAAGACAAAAACAAGATTTCT
CTTCATTATTTGGTTCNATCCAACCATTTCACCATTAACNAAACGAACCAGAAGAAAAAG
AAAAGTTTACCACTTTAA
Length of connected seq. 1208
Full length Seq ID -
Full length Seq. -
Length of full length seq. -