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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFJ246
Library AF
(Link to library)
Clone ID AFJ246
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16240-1
Original site URL
Representative seq. ID AFJ246P
(Link to Original site)
Representative DNA sequence 
>AFJ246 (AFJ246Q) /CSM/AF/AFJ2-B/AFJ246Q.Seq.d/
TCGTTGATGATCAAGGAGTTTTAATTGTAAATGATGAACAATCCTCTAATAAATGGTTCA
ATAGTGATGATCTTTGTTCAAATTATTTTTTAAGTTATCAATTAGATCTAGATCTTTGGG
GTAATTTTAATATAATAGGTGGTGGTGGACCATTTGCACCAACTGATGGTGTTGGATTTG
GTGACCCTAATCTATTCCCTGAGTATGCAAGTGATCGATTCAATCAGTATCATTGTTTTG
AGCTTCXXXXXXXXXXAACATCTGTATGTTTTGATGGTCAATGTTCAGTTTGTNAAGTTG
ATTCAAGCTCTGTTNATATTTCANCCACTGGTATAGTTACAACCACTGGCATTTCTATAT
CNACAATTGGTCCTGTATCAACANCTGATTTATCAATTTCAACAATTGGTTTTGTAACTA
CCACTGGTGTAACAGCTTCAACGATTGGCATTGTTACTACAACTGGTTTCATAACTTCAA
CAACTTCAACAANTGGTTTATTAACCACTGCTTCAACAACCTCAACAACTGGTATATTAA
CCACNGCTTCATCAACTTCAACAACGGGAANACCAACAATTTCAACATCAATTTCGACAT
CAGGAGCAACAGGAACAACAACAACCCCAACAACAGGTTCCACTGGAACAATACCACCTA
TTGACCTTGCACAATGAGTCATGATTCTTGCCCACCTCATTATTACTGTTATCAATTAAC
AAATTCATACACTTGTATATCAAAAGAATTTATAATA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
VDDQGVLIVNDEQSSNKWFNSDDLCSNYFLSYQLDLDLWGNFNIIGGGGPFAPTDGVGFG
DPNLFPEYASDRFNQYHCFEL---

---TSVCFDGQCSVCXVDSSSVXISXTGIVTTTGISISTIGPVSTXDLSISTIGFVTTTG
VTASTIGIVTTTGFITSTTSTXGLLTTASTTSTTGILTTASSTSTTGXPTISTSISTSGA
TGTTTTPTTGSTGTIPPIDLAQ*vmilahliitvin*qihtlvyqknl*
Translated Amino Acid sequence (All Frames) 
Frame A:
slmikef*l*mmnnplingsivmifvqiif*vin*i*ifgvili**vvvdhlhqlmvldl
vtliyslsmqvidsisiivlsf---

---nicmf*wsmfslxs*fklcxyfxhwysynhwhfyixnwscinx*finfnnwfcnyhw
cnsfndwhcyynwfhnfnnfnxwfinhcfnnlnnwyinhxfinfnngxtnnfninfdirs
nrnnnnpnnrfhwnntty*pctmshdscpphyycyqltnsytciskefii

Frame B:
r**srsfnck**til**mvq***slfklffklsirsrslg*f*ynrwwwtictn*wcwiw
*p*sip*vck*siqsvslf*a---

---TSVCFDGQCSVCXVDSSSVXISXTGIVTTTGISISTIGPVSTXDLSISTIGFVTTTG
VTASTIGIVTTTGFITSTTSTXGLLTTASTTSTTGILTTASSTSTTGXPTISTSISTSGA
TGTTTTPTTGSTGTIPPIDLAQ*vmilahliitvin*qihtlvyqknl*

Frame C:
VDDQGVLIVNDEQSSNKWFNSDDLCSNYFLSYQLDLDLWGNFNIIGGGGPFAPTDGVGFG
DPNLFPEYASDRFNQYHCFEL---

---hlyvlmvnvqfvkliqalxifxplv*lqplaflyxqlvlyqxliyqfqqlvl*lplv
*qlqrlalllqlvs*lqqlqqxvy*pllqqpqqlvy*pxlhqlqqrexqqfqhqfrhqeq
qeqqqpqqqvpleqyhlltlhnes*flptsllllsinkfihlyikriyn
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFJ246 (AFJ246Q) /CSM/AF/AFJ2-B/AFJ246Q.Seq.d/                       1394   0.0
AFJ146 (AFJ146Q) /CSM/AF/AFJ1-B/AFJ146Q.Seq.d/                        926   0.0
SLB129 (SLB129Q) /CSM/SL/SLB1-B/SLB129Q.Seq.d/                        912   0.0
SFL410 (SFL410Q) /CSM/SF/SFL4-A/SFL410Q.Seq.d/                        912   0.0
SFL391 (SFL391Q) /CSM/SF/SFL3-D/SFL391Q.Seq.d/                        912   0.0
SFJ534 (SFJ534Q) /CSM/SF/SFJ5-B/SFJ534Q.Seq.d/                        912   0.0
SFG761 (SFG761Q) /CSM/SF/SFG7-C/SFG761Q.Seq.d/                        912   0.0
SFE370 (SFE370Q) /CSM/SF/SFE3-C/SFE370Q.Seq.d/                        912   0.0
SFE249 (SFE249Q) /CSM/SF/SFE2-C/SFE249Q.Seq.d/                        912   0.0
SFE196 (SFE196Q) /CSM/SF/SFE1-D/SFE196Q.Seq.d/                        912   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    40   4e-06  15
AC116982|AC116982.2 Dictyostelium discoideum chromosome 2 map 3622643-3879522 strain AX4, complete sequence.    36   6e-06  12
AC115599|AC115599.2 Dictyostelium discoideum chromosome 2 map 4229098-4354721 strain AX4, complete sequence.    32   1e-05  12
AC116979|AC116979.3 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.    34   1e-05  16
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    36   7e-05  14
AC115592|AC115592.2 Dictyostelium discoideum chromosome 2 map 1-125959 strain AX4, complete sequence.    38   0.001  8
Y13097|Y13097.1 D.discoideum mRNA for stat protein, first finger stage.    40   0.003  3
AC122916|AC122916.4 Mus musculus chromosome 5 clone RP23-28F1, complete sequence.    38   0.006  7
AC116919|AC116919.2 Dictyostelium discoideum chromosome 2 map complement(6245035-6206343) strain AX4, complete sequence.    36   0.007  6
AC116963|AC116963.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.    36   0.014  11
dna update 2004. 9.24
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q4I7X1) RecName: Full=Polyadenylation factor subunit 2;               33   7.0
protein update 2009. 4.23
PSORT 

 psg: 0.75  gvh: 0.39  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 64.0 %: cytoplasmic
	 24.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for AFJ246 is cyt
5' end seq. ID AFJ246F
5' end seq. 
>AFJ246F.Seq
TCGTTGATGATCAAGGAGTTTTAATTGTAAATGATGAACAATCCTCTAATAAATGGTTCA
ATAGTGATGATCTTTGTTCAAATTATTTTTTAAGTTATCAATTAGATCTAGATCTTTGGG
GTAATTTTAATATAATAGGTGGTGGTGGACCATTTGCACCAACTGATGGTGTTGGATTTG
GTGACCCTAATCTATTCCCTGAGTATGCAAGTGATCGATTCAATCAGTATCATTGTTTTG
AGCTTC----------
Length of 5' end seq. 246
3' end seq. ID AFJ246Z
3' end seq. 
>AFJ246Z.Seq
----------AACATCTGTATGTTTTGATGGTCAATGTTCAGTTTGTNAAGTTGATTCAA
GCTCTGTTNATATTTCANCCACTGGTATAGTTACAACCACTGGCATTTCTATATCNACAA
TTGGTCCTGTATCAACANCTGATTTATCAATTTCAACAATTGGTTTTGTAACTACCACTG
GTGTAACAGCTTCAACGATTGGCATTGTTACTACAACTGGTTTCATAACTTCAACAACTT
CAACAANTGGTTTATTAACCACTGCTTCAACAACCTCAACAACTGGTATATTAACCACNG
CTTCATCAACTTCAACAACGGGAANACCAACAATTTCAACATCAATTTCGACATCAGGAG
CAACAGGAACAACAACAACCCCAACAACAGGTTCCACTGGAACAATACCACCTATTGACC
TTGCACAATGAGTCATGATTCTTGCCCACCTCATTATTACTGTTATCAATTAACAAATTC
ATACACTTGTATATCAAAAGAATTTATAATA
Length of 3' end seq. 501
Connected seq. ID AFJ246P
Connected seq. 
>AFJ246P.Seq
TCGTTGATGATCAAGGAGTTTTAATTGTAAATGATGAACAATCCTCTAATAAATGGTTCA
ATAGTGATGATCTTTGTTCAAATTATTTTTTAAGTTATCAATTAGATCTAGATCTTTGGG
GTAATTTTAATATAATAGGTGGTGGTGGACCATTTGCACCAACTGATGGTGTTGGATTTG
GTGACCCTAATCTATTCCCTGAGTATGCAAGTGATCGATTCAATCAGTATCATTGTTTTG
AGCTTC----------AACATCTGTATGTTTTGATGGTCAATGTTCAGTTTGTNAAGTTG
ATTCAAGCTCTGTTNATATTTCANCCACTGGTATAGTTACAACCACTGGCATTTCTATAT
CNACAATTGGTCCTGTATCAACANCTGATTTATCAATTTCAACAATTGGTTTTGTAACTA
CCACTGGTGTAACAGCTTCAACGATTGGCATTGTTACTACAACTGGTTTCATAACTTCAA
CAACTTCAACAANTGGTTTATTAACCACTGCTTCAACAACCTCAACAACTGGTATATTAA
CCACNGCTTCATCAACTTCAACAACGGGAANACCAACAATTTCAACATCAATTTCGACAT
CAGGAGCAACAGGAACAACAACAACCCCAACAACAGGTTCCACTGGAACAATACCACCTA
TTGACCTTGCACAATGAGTCATGATTCTTGCCCACCTCATTATTACTGTTATCAATTAAC
AAATTCATACACTTGTATATCAAAAGAATTTATAATA
Length of connected seq. 747
Full length Seq ID -
Full length Seq. -
Length of full length seq. -