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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHE140
Library SH
(Link to library)
Clone ID SHE140
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11247-1|Contig-U12475-1
Original site URL
Representative seq. ID SHE140P
(Link to Original site)
Representative DNA sequence 
>SHE140 (SHE140Q) /CSM/SH/SHE1-B/SHE140Q.Seq.d/
ACATGTATAATAATAATATATAAATATTTTTTTTTTATTTAATTTTTTATTTTTTTTTTN
AANCTTTTTTTATTTNATAANCCCANCTTTATTNATTNATTNATTNATTNATTNATTCTT
TTAAAAAANCCTATNTNTNTNGCCTTAAATTAAATATTATATAATTGGTNTNTTTTTACC
CCAAAAAAAAAAAAAAAAAAAAAAANTTTTNAAAAAAAAAXXXXXXXXXXAGTTTCAACT
GAACACCAAAGAGAATTGGCTGCTGATTTATTCACTTTCTTACCACCAGAATATGAAGCT
GGTGTTAAAGAATTTGACCAAGGAAAAGAAAGTGATAACTTTTGGAAAATTATTGGTGGT
AATTCTAATGATATTGTTATAAATACTTTTATAAATGAAAATAAAGAAGAAAAAGAAAAA
GAAGAAGAAGAAAAAGAAGAAGAGGAAGAAGAAGAGGAAGAAGAAGAAGAAGAAGAAGAA
GAAGAAAAAGATAATAATAAAACAACAACAATAATTAAACATTTAAGACCAAAGAAAATT
AAATTATTTTTATGCACAGATAATTCTGGTATCTTTAAAGCAGATCAAATTAATCCATTC
TCTCAAGTTGATTTAAATTCTCAAGAGTGTGTATTGTTAGATGTTTACCATAAAGTTTTC
CTTTG
sequence update 2002.10.25
Translated Amino Acid sequence 
hv***yinifflfnflfffxxffyxixpxlxixxxixxff*kxlxxxp*IKYYIIGXFLP
QKKKKKKKXXKKK---

---VSTEHQRELAADLFTFLPPEYEAGVKEFDQGKESDNFWKIIGGNSNDIVINTFINEN
KEEKEKEEEEKEEEEEEEEEEEEEEEEEKDNNKTTTIIKHLRPKKIKLFLCTDNSGIFKA
DQINPFSQVDLNSQECVLLDVYHKVFL
Translated Amino Acid sequence (All Frames) 
Frame A:
tciiiiykyfffi*ffiffxxlflfxxpxfixxxixxxillkxpxxxaln*ilynwxxft
pkkkkkkkxfxkk---

---sfn*tpkrigc*fihflttri*swc*ri*prkrk**llenyww*f**ycykyfyk*k
*rrkrkrrrrkrrrgrrrgrrrrrrrrrkr***nnnnn*tfktken*iifmhr*fwyl*s
rsn*silss*fkfsrvcivrclp*sfpl

Frame B:
hv***yinifflfnflfffxxffyxixpxlxixxxixxff*kxlxxxp*IKYYIIGXFLP
QKKKKKKKXXKKK---

---VSTEHQRELAADLFTFLPPEYEAGVKEFDQGKESDNFWKIIGGNSNDIVINTFINEN
KEEKEKEEEEKEEEEEEEEEEEEEEEEEKDNNKTTTIIKHLRPKKIKLFLCTDNSGIFKA
DQINPFSQVDLNSQECVLLDVYHKVFL

Frame C:
mynnni*ifffylifyfffxxffix*xxlyxxixxxixsfkkxyxxxlklnii*lvxfyp
kkkkkkkxfxkk---

---fqlntkenwlliyslsyhqnmklvlknltkekkvitfgkllvvilmill*ill*mki
kkkkkkkkkkkkkrkkkrkkkkkkkkkkkiiikqqq*lni*dqrklnyfyaqiilvslkq
iklihslkli*ilksvyc*mftikfsf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHE140 (SHE140Q) /CSM/SH/SHE1-B/SHE140Q.Seq.d/                        355   6e-97
AHC792 (AHC792Q) /CSM/AH/AHC7-D/AHC792Q.Seq.d/                        355   6e-97
VHB710 (VHB710Q) /CSM/VH/VHB7-A/VHB710Q.Seq.d/                        339   3e-92
CHE781 (CHE781Q) /CSM/CH/CHE7-D/CHE781Q.Seq.d/                        339   3e-92
SHL690 (SHL690Q) /CSM/SH/SHL6-D/SHL690Q.Seq.d/                        303   2e-81
VFM161 (VFM161Q) /CSM/VF/VFM1-C/VFM161Q.Seq.d/                        232   5e-60
SHK377 (SHK377Q) /CSM/SH/SHK3-D/SHK377Q.Seq.d/                         98   2e-19
VHG783 (VHG783Q) /CSM/VH/VHG7-D/VHG783Q.Seq.d/                         94   3e-18
VSK326 (VSK326Q) /CSM/VS/VSK3-B/VSK326Q.Seq.d/                         46   7e-04
VSI349 (VSI349Q) /CSM/VS/VSI3-C/VSI349Q.Seq.d/                         46   7e-04
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U95159|U95159.1 Dictyostelium discoideum gelsolin-related protein GRP125 gene, complete cds.   355   0.0    4
DQ021446|DQ021446.1 Daktulosphaira vitifoliae mitochondrion, partial genome.    38   6e-04  5
AZ192618|AZ192618.1 SP_1021_B1_C04_SP6E Strongylocentrotus purpuratus, purple sea urchin, sperm genomic BAC library Strongylocentrotus purpuratus genomic clone Plate=1021 Col=7 Row=F, DNA sequence.    42   0.003  2
CR382398|CR382398.1 Plasmodium falciparum chromosome 6, complete sequence; segment 1/5.    42   0.043  8
CT573220|CT573220.3 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone CH211-194O20.    50   0.062  1
CT025897|CT025897.3 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone.    50   0.062  1
AC115474|AC115474.4 Rattus norvegicus clone CH230-170D2, *** SEQUENCING IN PROGRESS ***, 6 unordered pieces.    50   0.062  1
AC110402|AC110402.4 Rattus norvegicus clone CH230-44K24, *** SEQUENCING IN PROGRESS ***, 5 unordered pieces.    50   0.062  1
AE017263|AE017263.1 Mesoplasma florum L1 complete genome.          40   0.075  10
AP003713|AP003713.3 Oryza sativa (japonica cultivar-group) genomic DNA, chromosome 6, PAC clone:P0528E12.    40   0.082  4
dna update 2006. 2. 9
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

T30330(T30330) gelsolin-related protein GRP125 - slime mold (Dic...   227   3e-58
(P36418) RecName: Full=Protovillin; AltName: Full=100 kDa actin-...    47   5e-04
DQ453500_1(DQ453500|pid:none) Heliocidaris tuberculata advillin ...    41   0.034
DQ453501_1(DQ453501|pid:none) Heliocidaris erythrogramma advilli...    41   0.034
AJ577853_1(AJ577853|pid:none) Nicotiana tabacum partial mRNA for...    40   0.059
(O81645) RecName: Full=Villin-3;  &AF081203_1(AF081203|pid:none)...    40   0.059
EU334660_1(EU334660|pid:none) Strongylocentrotus purpuratus vill...    40   0.077
DQ453502_1(DQ453502|pid:none) Strongylocentrotus purpuratus advi...    40   0.077
BC111730_1(BC111730|pid:none) Homo sapiens advillin, mRNA (cDNA ...    38   0.22
AK314362_1(AK314362|pid:none) Homo sapiens cDNA, FLJ95130, Homo ...    38   0.22
protein update 2009. 4.16
PSORT 

 psg: 0.89  gvh: 0.40  alm: 0.51  top: 1.07  tms: 0.00  mit: 0.27  mip: 0.00
 nuc: 0.30  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 28.0 %: mitochondrial
	 24.0 %: cytoplasmic
	  8.0 %: plasma membrane

>> prediction for SHE140 is nuc
5' end seq. ID SHE140F
5' end seq. 
>SHE140F.Seq
ACATGTATAATAATAATATATAAATATTTTTTTTTTATTTAATTTTTTATTTTTTTTTTN
AANCTTTTTTTATTTNATAANCCCANCTTTATTNATTNATTNATTNATTNATTNATTCTT
TTAAAAAANCCTATNTNTNTNGCCTTAAATTAAATATTATATAATTGGTNTNTTTTTACC
CCAAAAAAAAAAAAAAAAAAAAAAANTTTTNAAAAAAAAANNNNNNNNNN
Length of 5' end seq. 230
3' end seq. ID SHE140Z
3' end seq. 
>SHE140Z.Seq
NNNNNNNNNNAGTTTCAACTGAACACCAAAGAGAATTGGCTGCTGATTTATTCACTTTCT
TACCACCAGAATATGAAGCTGGTGTTAAAGAATTTGACCAAGGAAAAGAAAGTGATAACT
TTTGGAAAATTATTGGTGGTAATTCTAATGATATTGTTATAAATACTTTTATAAATGAAA
ATAAAGAAGAAAAAGAAAAAGAAGAAGAAGAAAAAGAAGAAGAGGAAGAAGAAGAGGAAG
AAGAAGAAGAAGAAGAAGAAGAAGAAAAAGATAATAATAAAACAACAACAATAATTAAAC
ATTTAAGACCAAAGAAAATTAAATTATTTTTATGCACAGATAATTCTGGTATCTTTAAAG
CAGATCAAATTAATCCATTCTCTCAAGTTGATTTAAATTCTCAAGAGTGTGTATTGTTAG
ATGTTTACCATAAAGTTTTCCTTTG
Length of 3' end seq. 445
Connected seq. ID SHE140P
Connected seq. 
>SHE140P.Seq
ACATGTATAATAATAATATATAAATATTTTTTTTTTATTTAATTTTTTATTTTTTTTTTN
AANCTTTTTTTATTTNATAANCCCANCTTTATTNATTNATTNATTNATTNATTNATTCTT
TTAAAAAANCCTATNTNTNTNGCCTTAAATTAAATATTATATAATTGGTNTNTTTTTACC
CCAAAAAAAAAAAAAAAAAAAAAAANTTTTNAAAAAAAAA----------AGTTTCAACT
GAACACCAAAGAGAATTGGCTGCTGATTTATTCACTTTCTTACCACCAGAATATGAAGCT
GGTGTTAAAGAATTTGACCAAGGAAAAGAAAGTGATAACTTTTGGAAAATTATTGGTGGT
AATTCTAATGATATTGTTATAAATACTTTTATAAATGAAAATAAAGAAGAAAAAGAAAAA
GAAGAAGAAGAAAAAGAAGAAGAGGAAGAAGAAGAGGAAGAAGAAGAAGAAGAAGAAGAA
GAAGAAAAAGATAATAATAAAACAACAACAATAATTAAACATTTAAGACCAAAGAAAATT
AAATTATTTTTATGCACAGATAATTCTGGTATCTTTAAAGCAGATCAAATTAATCCATTC
TCTCAAGTTGATTTAAATTCTCAAGAGTGTGTATTGTTAGATGTTTACCATAAAGTTTTC
CTTTG
Length of connected seq. 655
Full length Seq ID -
Full length Seq. -
Length of full length seq. -