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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHD894
Library SH
(Link to library)
Clone ID SHD894
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11926-1
Original site URL
Representative seq. ID SHD894P
(Link to Original site)
Representative DNA sequence 
>SHD894 (SHD894Q) /CSM/SH/SHD8-D/SHD894Q.Seq.d/
AAATGTACAATTGATGCATGTACCAAAGAAGGTGGTGTAACTCATACTCCAGTCAACACT
GATGATAACAATGCCTGTACAATTGATTCTTGTTCACCATCAACCGGTATTTCCCACACC
CCAATCAATTGTGATGATAAAAAGGCCTGTACAGTTGATTCATGTTCAAATTCAACTGGT
TGTGTAAATACTCCAATTTCCTGTGACGATAATAATCCATGTACTGTTGATTCTTGCGAT
GACTTAACTGGTTGTTGCAATACTCCAATCAATGTTGATGATAATAATCCATGTACCATC
GATGCCTGTACTAAATCAACAGGTGTTACTCATACCCCAGTCAATGTAGATGATAATAAC
AAATGTACAATTGATACATGTACCAAAGAAGGTGGTGTAACTCATACTCCAGTCAATACT
GATGATAACAATGCCTGTACCCTTGATTCCTGNTCACCATCAACTGGTGTTTCCCATACC
CCAATAAACTGTGATGATAATAATAAATGTACTGTAGATTCATGTTCAAACTCAACTGGT
TGTGTAAATACTCCAATTAACTGTGACGATAGTAATCCATGTACCGTAGACTCATGCANT
AATTCAACTGGTTGTGTAAACACTCCANTCAATGTTGATGATAATAATNCATGTACCGTC
GATGCCTGTACCAAATNAACAGGTGTTACTCATACCCCAATCAATGTGNATGATAACAAC
AAATGTACAATTGATGCATGTACCAAANAAAGGGGGGGTACTNATACCCCAATCAATACT
GATGATAACAATGCCTGTACAATTGATTCATGTNCAAAAAAAGGGGGNGTACCNNATACT
CCAATCAATCCTGGAGANAAAAAAGCCTGTACCCXXXXXXXXXXGCNAAACNTGNNAAAA
NTNAAAAACNNNTTTTANTTNATTNNATTTTNAAAAAANTCCAAAAAAANTCCAAAAAAA
ATTCNAGAAAAAAAAAAAAACTATTTGTTTTCTTATCTAATATTTATAATTTTTAAAAAT
AAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
KCTIDACTKEGGVTHTPVNTDDNNACTIDSCSPSTGISHTPINCDDKKACTVDSCSNSTG
CVNTPISCDDNNPCTVDSCDDLTGCCNTPINVDDNNPCTIDACTKSTGVTHTPVNVDDNN
KCTIDTCTKEGGVTHTPVNTDDNNACTLDSXSPSTGVSHTPINCDDNNKCTVDSCSNSTG
CVNTPINCDDSNPCTVDSCXNSTGCVNTPXNVDDNNXCTVDACTKXTGVTHTPINVXDNN
KCTIDACTKXRGGTXTPINTDDNNACTIDSCXKKGGVPXTPINPGXKKACT---

---XNXXKXKNXFXXIXFXKXSKKXPKKIXEKKKNYLFSYLIFIIFKNK
Translated Amino Acid sequence (All Frames) 
Frame A:
KCTIDACTKEGGVTHTPVNTDDNNACTIDSCSPSTGISHTPINCDDKKACTVDSCSNSTG
CVNTPISCDDNNPCTVDSCDDLTGCCNTPINVDDNNPCTIDACTKSTGVTHTPVNVDDNN
KCTIDTCTKEGGVTHTPVNTDDNNACTLDSXSPSTGVSHTPINCDDNNKCTVDSCSNSTG
CVNTPINCDDSNPCTVDSCXNSTGCVNTPXNVDDNNXCTVDACTKXTGVTHTPINVXDNN
KCTIDACTKXRGGTXTPINTDDNNACTIDSCXKKGGVPXTPINPGXKKACT---

---akxxkxxkxxlxxxixkkxqkxskknsrkkkklfvflsniynf*k*k

Frame B:
nvqlmhvpkkvv*lilqstlmitmpvqlilvhhqpvfptpqsivmikrpvqlihvqiqlv
v*ilqfpvtiiihvllilamt*lvvailqsmlmiiihvpsmpvlnqqvllipqsm*miit
nvqlihvpkkvv*lilqsilmitmpvplipxhhqlvfpipq*tvmiiinvl*ihvqtqlv
v*ilqltvtivihvp*thaxiqlvv*tlxsmlmiixhvpsmpvpnxqvllipqsmxmitt
nvqlmhvpxkggvlipqsilmitmpvqlihvqkkgxyxilqsilexkkpvp---

---XNXXKXKNXFXXIXFXKXSKKXPKKIXEKKKNYLFSYLIFIIFKNK

Frame C:
myn*cmyqrrwcnsyssqh***qclyn*flftinryfphpnql***kglys*fmfkfnwl
ckysnfl*r**smyc*flr*lnwllqysnqc****smyhrcly*inrcysypsqcr***q
myn*ymyqrrwcnsyssqy***qclyp*flxtinwcfpypnkl*****mycrfmfklnwl
ckysn*l*r**smyrrlmx*fnwlckhsxqc****xmyrrclyqxnrcysypnqcx**qq
myn*cmyqxkggyxypnqy***qclyn*fmxkkrgxtxysnqswrxksly---

---xtxxxxktxfxxxxfxkxpkkxqkkfxkkkkticfli*yl*flkik
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHD894 (SHD894Q) /CSM/SH/SHD8-D/SHD894Q.Seq.d/                       1522   0.0
SHA221 (SHA221Q) /CSM/SH/SHA2-A/SHA221Q.Seq.d/                       1481   0.0
SHC394 (SHC394Q) /CSM/SH/SHC3-D/SHC394Q.Seq.d/                       1372   0.0
SHE633 (SHE633Q) /CSM/SH/SHE6-B/SHE633Q.Seq.d/                       1334   0.0
SHH556 (SHH556Q) /CSM/SH/SHH5-C/SHH556Q.Seq.d/                       1298   0.0
SHK455 (SHK455Q) /CSM/SH/SHK4-C/SHK455Q.Seq.d/                       1174   0.0
CHR684 (CHR684Q) /CSM/CH/CHR6-D/CHR684Q.Seq.d/                       1110   0.0
SHK502 (SHK502Q) /CSM/SH/SHK5-A/SHK502Q.Seq.d/                       1108   0.0
SHF321 (SHF321Q) /CSM/SH/SHF3-A/SHF321Q.Seq.d/                       1074   0.0
SHA313 (SHA313Q) /CSM/SH/SHA3-A/SHA313Q.Seq.d/                       1041   0.0
own update 2004. 5.13
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X78948|X78948.1 D.minutum ecmB gene.                               88   2e-28  5
CK991559|CK991559.1 EST0111 Eyestalk cDNA library Penaeus monodon cDNA clone ES-N-S03-555-W 5' similar to Histidine-rich glycoprotein precursor (Plasmodium lophurae), mRNA sequence.    30   6e-04  5
BX231032|BX231032.1 Danio rerio genomic clone DKEY-254O18, genomic survey sequence.    30   0.018  5
AL805962|AL805962.5 Mouse DNA sequence from clone RP23-121I18 on chromosome X.    50   0.097  1
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    38   0.18   10
AC130411|AC130411.1 Homo sapiens chromosome 3 clone RP11-4M21, *** SEQUENCING IN PROGRESS ***, 6 ordered pieces.    48   0.38   1
AC012584|AC012584.6 Homo sapiens clone RP11-4M21, WORKING DRAFT SEQUENCE, 14 unordered pieces.    48   0.38   1
AC100096|AC100096.1 Mus musculus clone RP23-38A11, LOW-PASS SEQUENCE SAMPLING.    48   0.38   1
AC140238|AC140238.3 Mus musculus BAC clone RP24-279O7 from 3, complete sequence.    48   0.38   1
AC140237|AC140237.4 Mus musculus BAC clone RP24-279G5 from 3, complete sequence.    48   0.38   1
dna update 2006. 2. 9
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

A27020(A27020)DIF-induced prestalk pDd63 protein precursor - sli...   409   e-112
(P11976) RecName: Full=Prestalk protein; AltName: Full=Extracell...   399   e-110
A26838(A26838)prestalk protein precursor - slime mold (Dictyoste...   399   e-110
S44208(S44208) extracellular matrix protein B - Dictyostelium mi...   320   5e-86
AC117072_61(AC117072|pid:none) Dictyostelium discoideum chromoso...   293   8e-78
AC116984_103(AC116984|pid:none) Dictyostelium discoideum chromos...   287   4e-76
(Q54CH8) RecName: Full=Protein psiN; Flags: Precursor;                137   7e-31
(Q54G85) RecName: Full=Protein psiJ; Flags: Precursor;                136   1e-30
(Q54C31) RecName: Full=Protein psiR; Flags: Precursor;                130   9e-29
(Q54C32) RecName: Full=Protein psiQ; Flags: Precursor;                112   1e-23
protein update 2009. 4.16
PSORT 

 psg: 0.82  gvh: 0.44  alm: 0.52  top: 0.53  tms: 0.00  mit: 0.26  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 24.0 %: cytoplasmic
	 16.0 %: cytoskeletal
	 12.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for SHD894 is nuc
5' end seq. ID SHD894F
5' end seq. 
>SHD894F.Seq
AAATGTACAATTGATGCATGTACCAAAGAAGGTGGTGTAACTCATACTCCAGTCAACACT
GATGATAACAATGCCTGTACAATTGATTCTTGTTCACCATCAACCGGTATTTCCCACACC
CCAATCAATTGTGATGATAAAAAGGCCTGTACAGTTGATTCATGTTCAAATTCAACTGGT
TGTGTAAATACTCCAATTTCCTGTGACGATAATAATCCATGTACTGTTGATTCTTGCGAT
GACTTAACTGGTTGTTGCAATACTCCAATCAATGTTGATGATAATAATCCATGTACCATC
GATGCCTGTACTAAATCAACAGGTGTTACTCATACCCCAGTCAATGTAGATGATAATAAC
AAATGTACAATTGATACATGTACCAAAGAAGGTGGTGTAACTCATACTCCAGTCAATACT
GATGATAACAATGCCTGTACCCTTGATTCCTGNTCACCATCAACTGGTGTTTCCCATACC
CCAATAAACTGTGATGATAATAATAAATGTACTGTAGATTCATGTTCAAACTCAACTGGT
TGTGTAAATACTCCAATTAACTGTGACGATAGTAATCCATGTACCGTAGACTCATGCANT
AATTCAACTGGTTGTGTAAACACTCCANTCAATGTTGATGATAATAATNCATGTACCGTC
GATGCCTGTACCAAATNAACAGGTGTTACTCATACCCCAATCAATGTGNATGATAACAAC
AAATGTACAATTGATGCATGTACCAAANAAAGGGGGGGTACTNATACCCCAATCAATACT
GATGATAACAATGCCTGTACAATTGATTCATGTNCAAAAAAAGGGGGNGTACCNNATACT
CCAATCAATCCTGGAGANAAAAAAGCCTGTACCCNNNNNNNNNN
Length of 5' end seq. 884
3' end seq. ID SHD894Z
3' end seq. 
>SHD894Z.Seq
NNNNNNNNNNGCNAAACNTGNNAAAANTNAAAAACNNNTTTTANTTNATTNNATTTTNAA
AAAANTCCAAAAAAANTCCAAAAAAAATTCNAGAAAAAAAAAAAAACTATTTGTTTTCTT
ATCTAATATTTATAATTTTTAAAAATAAAAA
Length of 3' end seq. 151
Connected seq. ID SHD894P
Connected seq. 
>SHD894P.Seq
AAATGTACAATTGATGCATGTACCAAAGAAGGTGGTGTAACTCATACTCCAGTCAACACT
GATGATAACAATGCCTGTACAATTGATTCTTGTTCACCATCAACCGGTATTTCCCACACC
CCAATCAATTGTGATGATAAAAAGGCCTGTACAGTTGATTCATGTTCAAATTCAACTGGT
TGTGTAAATACTCCAATTTCCTGTGACGATAATAATCCATGTACTGTTGATTCTTGCGAT
GACTTAACTGGTTGTTGCAATACTCCAATCAATGTTGATGATAATAATCCATGTACCATC
GATGCCTGTACTAAATCAACAGGTGTTACTCATACCCCAGTCAATGTAGATGATAATAAC
AAATGTACAATTGATACATGTACCAAAGAAGGTGGTGTAACTCATACTCCAGTCAATACT
GATGATAACAATGCCTGTACCCTTGATTCCTGNTCACCATCAACTGGTGTTTCCCATACC
CCAATAAACTGTGATGATAATAATAAATGTACTGTAGATTCATGTTCAAACTCAACTGGT
TGTGTAAATACTCCAATTAACTGTGACGATAGTAATCCATGTACCGTAGACTCATGCANT
AATTCAACTGGTTGTGTAAACACTCCANTCAATGTTGATGATAATAATNCATGTACCGTC
GATGCCTGTACCAAATNAACAGGTGTTACTCATACCCCAATCAATGTGNATGATAACAAC
AAATGTACAATTGATGCATGTACCAAANAAAGGGGGGGTACTNATACCCCAATCAATACT
GATGATAACAATGCCTGTACAATTGATTCATGTNCAAAAAAAGGGGGNGTACCNNATACT
CCAATCAATCCTGGAGANAAAAAAGCCTGTACCC----------GCNAAACNTGNNAAAA
NTNAAAAACNNNTTTTANTTNATTNNATTTTNAAAAAANTCCAAAAAAANTCCAAAAAAA
ATTCNAGAAAAAAAAAAAAACTATTTGTTTTCTTATCTAATATTTATAATTTTTAAAAAT
AAAAA
Length of connected seq. 1015
Full length Seq ID -
Full length Seq. -
Length of full length seq. -