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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFJ181
Library AF
(Link to library)
Clone ID AFJ181
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16252-1
Original site URL
Representative seq. ID AFJ181P
(Link to Original site)
Representative DNA sequence 
>AFJ181 (AFJ181Q) /CSM/AF/AFJ1-D/AFJ181Q.Seq.d/
CTCCCACCCACTTATTACTTTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTA
AAATGAGCGTCATCAAATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAA
CTGTTGAAGTTGACCTTTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAG
TTCCATCAGGTGCATCAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAAT
CAAGATACTTAGGTAAAGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAAC
CAGCCGTCATTGGTAAATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATC
TCGATGGTACCCCAAATAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAG
CTGTTTGCAGAGCTGGTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAA
TTGCAGGTACTAAAATGAGATTACCAGTTCCAGCATTCAATGTTATCAATGGTGGTTCAC
ATGCAGGTAATXXXXXXXXXXGTGAAAAATTAGGTGATNTNTACANANAATTCATTAAAN
AANATCCNATCATTTCAATTGAANATCCNTTTGATCAANATGATTGGGAATCATACNCTN
AATTAACAGCATCAGTTGATATTCAAATTGTTGGTGATGATTTACTTGTTACCAACCCAN
AAANAATTAANACTGGTATTGAAAANAAAGCATGTAATGCTNTTTTATTAAAAGTTAACC
AAATTGGNTCAGTCNCTGAATCAATTNGNGCCGCTCTTGACTCAAANAATGCATCATGGG
GNGTCATGGTTANCCATCGNTCAGGTGAAACTGAANANACCTTCATT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
PTHLLLYFFLSLSIYISFFKMSVIKSIKAREILDSRGNPTVEVDLYTEKDGVVSSFRAAV
PSGASTGIYEAVELRDGDKSRYLGKGVLKAIKNILEVIQPAVIGKSVSDQAAIDKLMIDL
DGTPNKGKLGANAILAVSLAVCRAGAADRNLPLYKYISEIAGTKMRLPVPAFNVINGGSH
AGN---

---EKLGDXYXXFIKXXPIISIEXPFDQXDWESYXXLTASVDIQIVGDDLLVTNPXXIXT
GIEXKACNAXLLKVNQIGSVXESIXAALDSXNASWGVMVXHRSGETEXTFI
Translated Amino Acid sequence (All Frames) 
Frame A:
lpptyyfiffylylyiyhflk*assnqlkpekf*ihvvtqllkltftpkkmvlfhhselq
fhqvhqlvfmklln*emvinqdt*vkvyskqsktfsksfnqpslvnqfpikllsin**si
smvpqikvn*vptlslqsv*lfaelvllieiyhftnisqklqvlk*dyqfqhsmlsmvvh
mqv---

---vkn*vxxtxnslxxixsfqlxixlixmignhtln*qhqlifkllvmiyllptxkxlx
lvlkxkhvmlfy*kltklxqslnqxxplltqxmhhgxswlxixqvklxxps

Frame B:
shplitlfffisiyiyiif*nerhqin*sqrnfrftw*pnc*s*plhrkrwccfiiqscs
sircinwyl*sc*ikrw**ikilr*rctqsnqkhsrshstsrhw*isfrsscyr*indrs
rwypk*r*ircqrypcsqfsclqswcc**kfttlqiylrncry*neitsssiqcyqwwft
cr*---

---*kir*xxxxih*xxsxhfn*xsx*sx*lgiixxinsis*ysncw**ftcyqpxxn*x
wy*kxsm*cxfiks*pnwxsx*inxxrs*lkxcimgxhgxpsxr*n*xxlh

Frame C:
PTHLLLYFFLSLSIYISFFKMSVIKSIKAREILDSRGNPTVEVDLYTEKDGVVSSFRAAV
PSGASTGIYEAVELRDGDKSRYLGKGVLKAIKNILEVIQPAVIGKSVSDQAAIDKLMIDL
DGTPNKGKLGANAILAVSLAVCRAGAADRNLPLYKYISEIAGTKMRLPVPAFNVINGGSH
AGN---

---EKLGDXYXXFIKXXPIISIEXPFDQXDWESYXXLTASVDIQIVGDDLLVTNPXXIXT
GIEXKACNAXLLKVNQIGSVXESIXAALDSXNASWGVMVXHRSGETEXTFI
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFJ181 (AFJ181Q) /CSM/AF/AFJ1-D/AFJ181Q.Seq.d/                       1485   0.0
VFH161 (VFH161Q) /CSM/VF/VFH1-C/VFH161Q.Seq.d/                       1031   0.0
VFF123 (VFF123Q) /CSM/VF/VFF1-A/VFF123Q.Seq.d/                       1031   0.0
VFE233 (VFE233Q) /CSM/VF/VFE2-B/VFE233Q.Seq.d/                       1031   0.0
VFB851 (VFB851Q) /CSM/VF/VFB8-C/VFB851Q.Seq.d/                       1031   0.0
SSM734 (SSM734Q) /CSM/SS/SSM7-B/SSM734Q.Seq.d/                       1031   0.0
SHG555 (SHG555Q) /CSM/SH/SHG5-C/SHG555Q.Seq.d/                       1031   0.0
SFK326 (SFK326Q) /CSM/SF/SFK3-B/SFK326Q.Seq.d/                       1031   0.0
SFH339 (SFH339Q) /CSM/SF/SFH3-B/SFH339Q.Seq.d/                       1031   0.0
SFH308 (SFH308Q) /CSM/SF/SFH3-A/SFH308Q.Seq.d/                       1031   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BD183626|BD183626.1 Material for diagnosing malaria and immune antigen for suppressinggrowth of plasmodium.    62   6e-34  7
BD183625|BD183625.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    62   6e-34  7
AB026051|AB026051.1 Plasmodium falciparum mRNA for enolase, complete cds.    62   6e-34  7
U00152|U00152.1 Plasmodium falciparum enolase gene complete cds.    62   4e-32  6
BM165201|BM165201.1 EST567724 PyBS Plasmodium yoelii yoelii cDNA clone PYCMI87 5' end, mRNA sequence.    62   2e-27  4
AX619982|AX619982.1 Sequence 2945 from Patent WO02094868.          50   3e-24  6
AX932021|AX932021.1 Sequence 4 from Patent WO03087352.             50   3e-24  6
AX932030|AX932030.1 Sequence 13 from Patent WO03087352.            50   3e-24  6
BD237140|BD237140.1 Staphylococcus aureus gene and polypeptide.    50   5e-24  6
AR282358|AR282358.1 Sequence 21 from patent US 6521441.            50   5e-24  6
dna update 2004. 9.24
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54RK5) RecName: Full=Enolase A;          EC=4.2.1.11; AltName:...   313   5e-84
Z68318_5(Z68318|pid:none) Caenorhabditis elegans Cosmid T21B10, ...   284   3e-75
BC009018_1(BC009018|pid:none) Mus musculus enolase 2, gamma neur...   241   2e-62
EF137905_1(EF137905|pid:none) Helianthus annuus plastid enolase ...   219   1e-55
BC096516_1(BC096516|pid:none) Xenopus tropicalis hypothetical pr...   218   3e-55
BC045082_1(BC045082|pid:none) Xenopus laevis enolase 3, beta mus...   217   3e-55
U85835_1(U85835|pid:none) Unidentified pseudophyllidean enolase ...   217   5e-55
DQ497989_1(DQ497989|pid:none) Typhlonectes natans enolase B mRNA...   216   8e-55
AC016662_10(AC016662|pid:none) Arabidopsis thaliana chromosome 1...   215   1e-54
(Q9U615) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   215   2e-54
protein update 2009. 4.23
PSORT 

 psg: 0.95  gvh: 0.63  alm: 0.32  top: 0.57  tms: 0.07  mit: 0.36  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.05  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 44.0 %: nuclear
	 28.0 %: cytoplasmic
	  8.0 %: Golgi
	  8.0 %: mitochondrial
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for AFJ181 is nuc
5' end seq. ID AFJ181F
5' end seq. 
>AFJ181F.Seq
CTCCCACCCACTTATTACTTTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTA
AAATGAGCGTCATCAAATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAA
CTGTTGAAGTTGACCTTTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAG
TTCCATCAGGTGCATCAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAAT
CAAGATACTTAGGTAAAGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAAC
CAGCCGTCATTGGTAAATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATC
TCGATGGTACCCCAAATAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAG
CTGTTTGCAGAGCTGGTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAA
TTGCAGGTACTAAAATGAGATTACCAGTTCCAGCATTCAATGTTATCAATGGTGGTTCAC
ATGCAGGTAAT----------
Length of 5' end seq. 551
3' end seq. ID AFJ181Z
3' end seq. 
>AFJ181Z.Seq
----------GTGAAAAATTAGGTGATNTNTACANANAATTCATTAAANAANATCCNATC
ATTTCAATTGAANATCCNTTTGATCAANATGATTGGGAATCATACNCTNAATTAACAGCA
TCAGTTGATATTCAAATTGTTGGTGATGATTTACTTGTTACCAACCCANAAANAATTAAN
ACTGGTATTGAAAANAAAGCATGTAATGCTNTTTTATTAAAAGTTAACCAAATTGGNTCA
GTCNCTGAATCAATTNGNGCCGCTCTTGACTCAAANAATGCATCATGGGGNGTCATGGTT
ANCCATCGNTCAGGTGAAACTGAANANACCTTCATT
Length of 3' end seq. 326
Connected seq. ID AFJ181P
Connected seq. 
>AFJ181P.Seq
CTCCCACCCACTTATTACTTTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTA
AAATGAGCGTCATCAAATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAA
CTGTTGAAGTTGACCTTTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAG
TTCCATCAGGTGCATCAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAAT
CAAGATACTTAGGTAAAGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAAC
CAGCCGTCATTGGTAAATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATC
TCGATGGTACCCCAAATAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAG
CTGTTTGCAGAGCTGGTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAA
TTGCAGGTACTAAAATGAGATTACCAGTTCCAGCATTCAATGTTATCAATGGTGGTTCAC
ATGCAGGTAAT----------GTGAAAAATTAGGTGATNTNTACANANAATTCATTAAAN
AANATCCNATCATTTCAATTGAANATCCNTTTGATCAANATGATTGGGAATCATACNCTN
AATTAACAGCATCAGTTGATATTCAAATTGTTGGTGATGATTTACTTGTTACCAACCCAN
AAANAATTAANACTGGTATTGAAAANAAAGCATGTAATGCTNTTTTATTAAAAGTTAACC
AAATTGGNTCAGTCNCTGAATCAATTNGNGCCGCTCTTGACTCAAANAATGCATCATGGG
GNGTCATGGTTANCCATCGNTCAGGTGAAACTGAANANACCTTCATT
Length of connected seq. 877
Full length Seq ID -
Full length Seq. -
Length of full length seq. -