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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFJ180
Library AF
(Link to library)
Clone ID AFJ180
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16444-1
Original site URL
Representative seq. ID AFJ180Z
(Link to Original site)
Representative DNA sequence 
>AFJ180 (AFJ180Q) /CSM/AF/AFJ1-D/AFJ180Q.Seq.d/
XXXXXXXXXXCCTCAACTTAATAGATCATGTCGTTTCAAATCATGCAGATAAAATGATGG
AACCAGTCGTTCAATGGTACGAAAAGGTTTTACAATTCCACCGTTTCTGGTCAGTTGATG
ACAAAACCATTCATACCGAATATTCATCATTAAGATCAGTCGTAGTTGCTGATAAGTCTG
AAAAAGTTAAATTACCAATTAATGAACCAGCCAATGGTATTAGAAAGAGTCAAATTCAAG
AATACGTAGATTTCTACAATGGTGCTGGTGTTCAACATATCGCCTTAAAGACTGATAACA
TCATTGATGCTATCTCAAAATTAAGATCTCGTGGTGTCTCTTTCCTCACTGTTCCAAAAA
CATACTATACATCACTCAGAGAGAAATTACAACACTCTTCATTAGAAATTAAAGAAGATT
TGGACACTTTAGAGAAATTACACATTTTAATCGATTATGATGACAAAGGTTATCTTTTAC
AAATCTTTACAAATAATGTTGAAGATAAACCAACTGTTTTCTTTGAAATTATCCAAAGAA
ACAACCATGATGGTTTCGGTGCTGGTAACTTTAAATCCCTCTTTGAAGCAATCGAAAGAC
AACAAGAAACTCGTGGAAACTTATAGGTGTGTCTCAATTACAAATGTTCTTTATTATAAC
TAAATAACATTACAATATATCAAAATAAAATAAATAAACTCNATTAAAAAGNATTTTT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
---LNLIDHVVSNHADKMMEPVVQWYEKVLQFHRFWSVDDKTIHTEYSSLRSVVVADKSE
KVKLPINEPANGIRKSQIQEYVDFYNGAGVQHIALKTDNIIDAISKLRSRGVSFLTVPKT
YYTSLREKLQHSSLEIKEDLDTLEKLHILIDYDDKGYLLQIFTNNVEDKPTVFFEIIQRN
NHDGFGAGNFKSLFEAIERQQETRGNL*vclnykcsll*lnnitiyqnkinklx*kxf
Translated Amino Acid sequence (All Frames) 
Frame A:
---LNLIDHVVSNHADKMMEPVVQWYEKVLQFHRFWSVDDKTIHTEYSSLRSVVVADKSE
KVKLPINEPANGIRKSQIQEYVDFYNGAGVQHIALKTDNIIDAISKLRSRGVSFLTVPKT
YYTSLREKLQHSSLEIKEDLDTLEKLHILIDYDDKGYLLQIFTNNVEDKPTVFFEIIQRN
NHDGFGAGNFKSLFEAIERQQETRGNL*vclnykcsll*lnnitiyqnkinklx*kxf


Frame B:
---st**imsfqimqik*wnqsfngtkrfynstvsgqlmtkpfipnihh*dqs*llislk
klnyqlmnqpmvlervkfknt*istmvlvfnisp*rlitslmlsqn*dlvvslsslfqkh
tihhsernyntlh*klkkiwtl*rnytf*simmtkvifykslqimlkinqlfslklsket
tmmvsvlvtlnpslkqskdnkklvetyrcvsitnvlyyn*itlqyikik*insikkxf


Frame C:
---qlnrscrfkscr*ndgtsrsmvrkgftippflvs**qnhsyrifiikisrsc**v*k
s*itn**tsqwy*kesnsrirrflqwcwcstyrlkd**hh*cylkikiswclfphcskni
lyitqreittlfirn*rrfghfreithfnrl**qrlsftnlyk*c*r*tncfl*nypkkq
p*wfrcw*l*ipl*snrkttrnswkligvsqlqmffiitk*hynisk*nk*txlkxif
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFJ180 (AFJ180Q) /CSM/AF/AFJ1-D/AFJ180Q.Seq.d/                       1392   0.0
VFA816 (VFA816Q) /CSM/VF/VFA8-A/VFA816Q.Seq.d/                       1348   0.0
CFG395 (CFG395Q) /CSM/CF/CFG3-D/CFG395Q.Seq.d/                       1348   0.0
CFB319 (CFB319Q) /CSM/CF/CFB3-A/CFB319Q.Seq.d/                       1348   0.0
VFK407 (VFK407Q) /CSM/VF/VFK4-A/VFK407Q.Seq.d/                       1346   0.0
SFC722 (SFC722Q) /CSM/SF/SFC7-A/SFC722Q.Seq.d/                       1346   0.0
VFL704 (VFL704Q) /CSM/VF/VFL7-A/VFL704Q.Seq.d/                       1342   0.0
CFI470 (CFI470Q) /CSM/CF/CFI4-C/CFI470Q.Seq.d/                       1340   0.0
VFM130 (VFM130Q) /CSM/VF/VFM1-B/VFM130Q.Seq.d/                       1338   0.0
VFK534 (VFK534Q) /CSM/VF/VFK5-B/VFK534Q.Seq.d/                       1338   0.0
own update 2001.11.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC117081|AC117081.2 Dictyostelium discoideum chromosome 2 map 5862124-6045772 strain AX4, complete sequence.  1283   0.0    13
BI322343|BI322343.1 kx19h02.y3 Parastrongyloides trichosuri FL pAMP1 v1 Chiapelli McCarter Parastrongyloides trichosuri cDNA 5' similar to SW:HPPD_CAEEL Q22633 4-HYDROXYPHENYLPYRUVATE DIOXYGENASE ;, mRNA sequence.    42   4e-10  4
BI502176|BI502176.1 kt86c08.y1 Strongyloides ratti L2 pAMP1 v1 Chiapelli McCarter Strongyloides ratti cDNA 5' similar to SW:HPPD_CAEEL Q22633 4-HYDROXYPHENYLPYRUVATE DIOXYGENASE ;, mRNA sequence.    36   9e-10  5
CO006156|CO006156.1 EST794491 Coccidioides posadasii spherule cDNA library, 0.4 to 2.3 kb Coccidioides posadasii cDNA clone CIEAT15 3' end, mRNA sequence.    52   5e-08  2
CO013595|CO013595.1 EST801930 Coccidioides posadasii spherule cDNA library, 0.4 to 2.3 kb Coccidioides posadasii cDNA clone CIEBZ67 5' end, mRNA sequence.    52   6e-08  2
CO005676|CO005676.1 EST794011 Coccidioides posadasii spherule cDNA library, 0.4 to 2.3 kb Coccidioides posadasii cDNA clone CIEAQ39 3' end, mRNA sequence.    52   6e-08  2
CO009511|CO009511.1 EST797846 Coccidioides posadasii spherule cDNA library, 0.4 to 2.3 kb Coccidioides posadasii cDNA clone CIEBC24 5' end, mRNA sequence.    52   7e-08  2
CO009510|CO009510.1 EST797845 Coccidioides posadasii spherule cDNA library, 0.4 to 2.3 kb Coccidioides posadasii cDNA clone CIEBC24 3' end, mRNA sequence.    52   7e-08  2
L38493|L38493.1 Coccidioides immitis T-cell reactive protein (trcP) gene exons 1-4, complete cds.    52   1e-07  2
CO005677|CO005677.1 EST794012 Coccidioides posadasii spherule cDNA library, 0.4 to 2.3 kb Coccidioides posadasii cDNA clone CIEAQ39 5' end, mRNA sequence.    52   1e-06  2
dna update 2004. 9.24
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q76NV5) RecName: Full=4-hydroxyphenylpyruvate dioxygenase;     ...   410   e-113
AM114773_1(AM114773|pid:none) Platynereis dumerilii partial mRNA...   291   1e-77
AE014296_3563(AE014296|pid:none) Drosophila melanogaster chromos...   291   1e-77
BC046075_1(BC046075|pid:none) Danio rerio zgc:56326, mRNA (cDNA ...   284   2e-75
(Q6TGZ5) RecName: Full=4-hydroxyphenylpyruvate dioxygenase;     ...   284   2e-75
S32821(S32821;S35890;S35889)4-hydroxyphenylpyruvate dioxygenase ...   282   6e-75
(Q02110) RecName: Full=4-hydroxyphenylpyruvate dioxygenase;     ...   282   6e-75
BC153801_1(BC153801|pid:none) Xenopus laevis hypothetical protei...   281   1e-74
BC077167_1(BC077167|pid:none) Danio rerio 4-hydroxyphenylpyruvat...   280   2e-74
(P32754) RecName: Full=4-hydroxyphenylpyruvate dioxygenase;     ...   279   7e-74
protein update 2009. 4.23
PSORT 

 psg: 0.75  gvh: 0.33  alm: 0.51  top: 0.53  tms: 0.00  mit: 0.23  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 36.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  8.0 %: vacuolar

>> prediction for AFJ180 is nuc
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID AFJ180Z
3' end seq. 
>AFJ180Z.Seq
----------CCTCAACTTAATAGATCATGTCGTTTCAAATCATGCAGATAAAATGATGG
AACCAGTCGTTCAATGGTACGAAAAGGTTTTACAATTCCACCGTTTCTGGTCAGTTGATG
ACAAAACCATTCATACCGAATATTCATCATTAAGATCAGTCGTAGTTGCTGATAAGTCTG
AAAAAGTTAAATTACCAATTAATGAACCAGCCAATGGTATTAGAAAGAGTCAAATTCAAG
AATACGTAGATTTCTACAATGGTGCTGGTGTTCAACATATCGCCTTAAAGACTGATAACA
TCATTGATGCTATCTCAAAATTAAGATCTCGTGGTGTCTCTTTCCTCACTGTTCCAAAAA
CATACTATACATCACTCAGAGAGAAATTACAACACTCTTCATTAGAAATTAAAGAAGATT
TGGACACTTTAGAGAAATTACACATTTTAATCGATTATGATGACAAAGGTTATCTTTTAC
AAATCTTTACAAATAATGTTGAAGATAAACCAACTGTTTTCTTTGAAATTATCCAAAGAA
ACAACCATGATGGTTTCGGTGCTGGTAACTTTAAATCCCTCTTTGAAGCAATCGAAAGAC
AACAAGAAACTCGTGGAAACTTATAGGTGTGTCTCAATTACAAATGTTCTTTATTATAAC
TAAATAACATTACAATATATCAAAATAAAATAAATAAACTCNATTAAAAAGNATTTTT
Length of 3' end seq. 708
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -