>SHD651 (SHD651Q) /CSM/SH/SHD6-C/SHD651Q.Seq.d/
ATAATAAAAAACGAACAACCCAATTTAGAAAATGGCCACATCCTATTCGGCTAATAATGG
TCAAAATGAAGAATATACATTTGATGCATTTGAAGATGGTTCATCTAAATTGGTTGGAGA
GCAACAATTAACTAAACTTAATCAATTTGCAGTTGATTATGCACAACAAATTTGGAAGAT
AGAAGAAGCATTAGATGAAACATTAAATGAAGTATGGGATATAAATATAGATCCAGTTTC
AATTTATAATAAACCTCATGAACAATTAGATTTAATTGAATTGGTTAAAACTGATCGTAA
AATTTTCAATAAAGTAATATTAGTATTCTCTTCAATTTGTAATCAAACTAGAATTTTAAA
AGAAACAGCAGAAAGTAAATTTTATTCACCATTAACAGTATTTGGAGAGATTACAGGTGA
ATCATCAGAAGGTGATGTACAAATTGAAGTTGGTAAATTATTACCATTTATGATTGATTT
ATCAGCATTTGTAAATAGATGTTATTCATTAATTAGAAATATTATTAGTCAATTTGCATC
GATCTATCAATCACAAAAGAATATACATACACAATTCTTTAAGAATGTTCATTTACAAGC
TGTCTATTATTCAATGATTGATATATTTTCAGTATTAATAAATTTAGATAGTATAATTAC
ACAAAATACTGCTTTAGAXXXXXXXXXXGACAGAGTTGGAAAGAGACATTAGACAATTGG
GTGTCTCCGAGACTGGTTTAACCTTTTTAGATCATTTTAGACTTTTAATCACTCATATTG
GTAATGCTATGGGTTATATTAGATTGGTCAGATCCGGTGGTTTACACTATTGTAGTAATG
CTATCAAATTCGTACCAGACTTGAAAAAGATTCCAAAATTCCAAGATTTAACCTCAAAAG
ATGCCCTCTCACCTGAAACAATTCAAGCTAGTACCAATTTGGATTCAATCATTCATAATC
TTTCAAATAATTTATCAGAGGGTACTGAATACTTTAAGATGTTGGTAAACGTTTTCGCTA
CAGAGTTTCGAAATATCGCTAATCAACATTTAAAGAATTTCTATATCATCGTACCTGCCT
TGGCCTCAAATTACATCGATCATATGATCAATAGTAAAGATAAACTCTTTAAGAAATCAA
AAGCAATCGGTGCTGAAGCTTTATTATTCACTGATGATGGCTTTGCAATTGGTTTGGCTT
ATATCTTAAAATTATTAGATCAAAATAAAGATTTCGATTCTCTTCATTGGTTCGATCGTA
TTCAATTGAAATGTGAAACTGATCAAAAGAGAATGTTGGCTGAAGCAAATGCAAAAGGTA
TCAAAGAGGATCAAAATCATTTAGCAATTAAAAAGATTCAAAATTTCCAACAAGAATATG
AATTATTT

**ktnnpi*KMATSYSANNGQNEEYTFDAFEDGSSKLVGEQQLTKLNQFAVDYAQQIWKI
EEALDETLNEVWDINIDPVSIYNKPHEQLDLIELVKTDRKIFNKVILVFSSICNQTRILK
ETAESKFYSPLTVFGEITGESSEGDVQIEVGKLLPFMIDLSAFVNRCYSLIRNIISQFAS
IYQSQKNIHTQFFKNVHLQAVYYSMIDIFSVLINLDSIITQNTAL---

---TELERDIRQLGVSETGLTFLDHFRLLITHIGNAMGYIRLVRSGGLHYCSNAIKFVPD
LKKIPKFQDLTSKDALSPETIQASTNLDSIIHNLSNNLSEGTEYFKMLVNVFATEFRNIA
NQHLKNFYIIVPALASNYIDHMINSKDKLFKKSKAIGAEALLFTDDGFAIGLAYILKLLD
QNKDFDSLHWFDRIQLKCETDQKRMLAEANAKGIKEDQNHLAIKKIQNFQQEYELF

Frame A:
iikneqpnlenghilfg**wsk*riyi*ci*rwfi*igwratin*t*sics*lcttnled
rrsir*nik*smgykyrssfnl**ts*tirfn*ig*n*s*nfq*snisilfnl*sn*nfk
rnsrk*ilftinsiwrdyr*iirr*ctn*sw*iitiyd*fisick*mlfin*kyy*sici
dlsitkeytytil*ecsftscllfnd*yifsinkfr*ynytkycfr---

---drvgkrh*tigclrdwfnlfrsf*tfnhsyw*cygly*igqirwftll**cyqirtr
lekdskiprfnlkrcplt*nnss*yqfgfnhs*sfk*firgy*il*dvgkrfryrvskyr
*stfkeflyhrtclglklhrsydq**r*tl*eiksnrc*sfiih**wlcnwfglylkiir
sk*rfrfsslvrsysiem*n*skenvg*skckryqrgsksfsn*kdskfptri*ii

Frame B:
**ktnnpi*KMATSYSANNGQNEEYTFDAFEDGSSKLVGEQQLTKLNQFAVDYAQQIWKI
EEALDETLNEVWDINIDPVSIYNKPHEQLDLIELVKTDRKIFNKVILVFSSICNQTRILK
ETAESKFYSPLTVFGEITGESSEGDVQIEVGKLLPFMIDLSAFVNRCYSLIRNIISQFAS
IYQSQKNIHTQFFKNVHLQAVYYSMIDIFSVLINLDSIITQNTAL---

---TELERDIRQLGVSETGLTFLDHFRLLITHIGNAMGYIRLVRSGGLHYCSNAIKFVPD
LKKIPKFQDLTSKDALSPETIQASTNLDSIIHNLSNNLSEGTEYFKMLVNVFATEFRNIA
NQHLKNFYIIVPALASNYIDHMINSKDKLFKKSKAIGAEALLFTDDGFAIGLAYILKLLD
QNKDFDSLHWFDRIQLKCETDQKRMLAEANAKGIKEDQNHLAIKKIQNFQQEYELF

Frame C:
nkkrttqfrkwphpirlimvkmknihlmhlkmvhlnwlesnn*lnlinlqlimhnkfgr*
kkh*mkh*mkygi*i*iqfqfiinlmnn*i*lnwlklivkfsik*y*yslqfviklef*k
kqqkvnfihh*qylerlqvnhqkvmyklklvnyyhl*liyqhl*idvih*leillvnlhr
sinhkriyihnslrmfiyklsiiq*liyfqy**i*iv*lhkill*---

---qswketldnwvsprlv*pf*iildf*slilvmlwvildwsdpvvytivvmlsnsyqt
*krfqnski*pqkmpshlkqfklvpiwiqsfiifqiiyqrvlntlrcw*tfslqsfeisl
ini*risissylpwpqitsii*sivkinslrnqkqsvlklyyslmmalqlvwlis*ny*i
kikisilfigsivfn*nvklikrecwlkqmqkvskrikii*qlkrfkisnknmny

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHD651 (SHD651Q) /CSM/SH/SHD6-C/SHD651Q.Seq.d/                       2692   0.0
VHK538 (VHK538Q) /CSM/VH/VHK5-B/VHK538Q.Seq.d/                       1388   0.0
VHB189 (VHB189Q) /CSM/VH/VHB1-D/VHB189Q.Seq.d/                       1388   0.0
SHL542 (SHL542Q) /CSM/SH/SHL5-B/SHL542Q.Seq.d/                       1388   0.0
SHD893 (SHD893Q) /CSM/SH/SHD8-D/SHD893Q.Seq.d/                       1388   0.0
SHD671 (SHD671Q) /CSM/SH/SHD6-C/SHD671Q.Seq.d/                       1388   0.0
CHG465 (CHG465Q) /CSM/CH/CHG4-C/CHG465Q.Seq.d/                       1388   0.0
CHG147 (CHG147Q) /CSM/CH/CHG1-B/CHG147Q.Seq.d/                       1388   0.0
AHL641 (AHL641Q) /CSM/AH/AHL6-B/AHL641Q.Seq.d/                       1388   0.0
AHC187 (AHC187Q) /CSM/AH/AHC1-D/AHC187Q.Seq.d/                       1388   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BU746157|BU746157.1 CH3#002_C07T7 Canine heart normalized cDNA Library in pBluescriptCanis familiaris cDNA clone CH3#002_C07 5', mRNA sequence.    38   7e-04  3
BY464171|BY464171.1 Mus musculus 15 days pregnant adult female amnion cDNA, RIKEN full-length enriched library, clone:K630150M24, 3' end partial sequence.    42   0.027  2
CP000123|CP000123.1 Mycoplasma capricolum subsp. capricolum ATCC 27343, complete genome.    42   0.029  20
AK113047|AK113047.1 Ciona intestinalis cDNA, clone:ciad002a21, full insert sequence.    48   0.031  2
AC168241|AC168241.2 Bos taurus clone CH240-197K22, *** SEQUENCING IN PROGRESS ***, 28 unordered pieces.    52   0.033  1
CR450713|CR450713.2 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone.    38   0.097  8
BB822673|BB822673.1 Mus musculus mammary gland RCB-0526 Jyg-MC(A) cDNA, RIKEN full-length enriched library, clone:G830022O04, 3' end partial sequence.    32   0.11   4
DN287505|DN287505.1 1241755 MARC 7BOV Bos taurus cDNA 3', mRNA sequence.    34   0.12   3
CR103667|CR103667.1 Forward strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP142o16.    50   0.13   1
CR044130|CR044130.1 Forward strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP186g09.    50   0.13   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54R74) RecName: Full=UPF0681 protein;                               465   e-129
BC040936_1(BC040936|pid:none) Homo sapiens KIAA1033, mRNA (cDNA ...   234   5e-60
AB028956_1(AB028956|pid:none) Homo sapiens mRNA for KIAA1033 pro...   234   7e-60
BC143373_1(BC143373|pid:none) Homo sapiens KIAA1033, mRNA (cDNA ...   234   7e-60
BC020314_1(BC020314|pid:none) Mus musculus RIKEN cDNA A230046K03...   232   3e-59
(Q3UMB9) RecName: Full=UPF0681 protein KIAA1033;                      232   3e-59
AK161247_1(AK161247|pid:none) Mus musculus adult male testis cDN...   232   3e-59
AK145000_1(AK145000|pid:none) Mus musculus mammary gland RCB-052...   232   3e-59
BC110850_1(BC110850|pid:none) Homo sapiens KIAA1033, mRNA (cDNA ...   174   6e-42
CP000600_221(CP000600|pid:none) Ostreococcus lucimarinus CCE9901...   170   9e-41

 psg: 0.79  gvh: 0.25  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 40.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: vacuolar
	  4.0 %: plasma membrane

>> prediction for SHD651 is nuc

>SHD651F.Seq
ATAATAAAAAACGAACAACCCAATTTAGAAAATGGCCACATCCTATTCGGCTAATAATGG
TCAAAATGAAGAATATACATTTGATGCATTTGAAGATGGTTCATCTAAATTGGTTGGAGA
GCAACAATTAACTAAACTTAATCAATTTGCAGTTGATTATGCACAACAAATTTGGAAGAT
AGAAGAAGCATTAGATGAAACATTAAATGAAGTATGGGATATAAATATAGATCCAGTTTC
AATTTATAATAAACCTCATGAACAATTAGATTTAATTGAATTGGTTAAAACTGATCGTAA
AATTTTCAATAAAGTAATATTAGTATTCTCTTCAATTTGTAATCAAACTAGAATTTTAAA
AGAAACAGCAGAAAGTAAATTTTATTCACCATTAACAGTATTTGGAGAGATTACAGGTGA
ATCATCAGAAGGTGATGTACAAATTGAAGTTGGTAAATTATTACCATTTATGATTGATTT
ATCAGCATTTGTAAATAGATGTTATTCATTAATTAGAAATATTATTAGTCAATTTGCATC
GATCTATCAATCACAAAAGAATATACATACACAATTCTTTAAGAATGTTCATTTACAAGC
TGTCTATTATTCAATGATTGATATATTTTCAGTATTAATAAATTTAGATAGTATAATTAC
ACAAAATACTGCTTTAGANNNNNNNNNN

>SHD651Z.Seq
NNNNNNNNNNGACAGAGTTGGAAAGAGACATTAGACAATTGGGTGTCTCCGAGACTGGTT
TAACCTTTTTAGATCATTTTAGACTTTTAATCACTCATATTGGTAATGCTATGGGTTATA
TTAGATTGGTCAGATCCGGTGGTTTACACTATTGTAGTAATGCTATCAAATTCGTACCAG
ACTTGAAAAAGATTCCAAAATTCCAAGATTTAACCTCAAAAGATGCCCTCTCACCTGAAA
CAATTCAAGCTAGTACCAATTTGGATTCAATCATTCATAATCTTTCAAATAATTTATCAG
AGGGTACTGAATACTTTAAGATGTTGGTAAACGTTTTCGCTACAGAGTTTCGAAATATCG
CTAATCAACATTTAAAGAATTTCTATATCATCGTACCTGCCTTGGCCTCAAATTACATCG
ATCATATGATCAATAGTAAAGATAAACTCTTTAAGAAATCAAAAGCAATCGGTGCTGAAG
CTTTATTATTCACTGATGATGGCTTTGCAATTGGTTTGGCTTATATCTTAAAATTATTAG
ATCAAAATAAAGATTTCGATTCTCTTCATTGGTTCGATCGTATTCAATTGAAATGTGAAA
CTGATCAAAAGAGAATGTTGGCTGAAGCAAATGCAAAAGGTATCAAAGAGGATCAAAATC
ATTTAGCAATTAAAAAGATTCAAAATTTCCAACAAGAATATGAATTATTT

>SHD651P.Seq
ATAATAAAAAACGAACAACCCAATTTAGAAAATGGCCACATCCTATTCGGCTAATAATGG
TCAAAATGAAGAATATACATTTGATGCATTTGAAGATGGTTCATCTAAATTGGTTGGAGA
GCAACAATTAACTAAACTTAATCAATTTGCAGTTGATTATGCACAACAAATTTGGAAGAT
AGAAGAAGCATTAGATGAAACATTAAATGAAGTATGGGATATAAATATAGATCCAGTTTC
AATTTATAATAAACCTCATGAACAATTAGATTTAATTGAATTGGTTAAAACTGATCGTAA
AATTTTCAATAAAGTAATATTAGTATTCTCTTCAATTTGTAATCAAACTAGAATTTTAAA
AGAAACAGCAGAAAGTAAATTTTATTCACCATTAACAGTATTTGGAGAGATTACAGGTGA
ATCATCAGAAGGTGATGTACAAATTGAAGTTGGTAAATTATTACCATTTATGATTGATTT
ATCAGCATTTGTAAATAGATGTTATTCATTAATTAGAAATATTATTAGTCAATTTGCATC
GATCTATCAATCACAAAAGAATATACATACACAATTCTTTAAGAATGTTCATTTACAAGC
TGTCTATTATTCAATGATTGATATATTTTCAGTATTAATAAATTTAGATAGTATAATTAC
ACAAAATACTGCTTTAGA----------GACAGAGTTGGAAAGAGACATTAGACAATTGG
GTGTCTCCGAGACTGGTTTAACCTTTTTAGATCATTTTAGACTTTTAATCACTCATATTG
GTAATGCTATGGGTTATATTAGATTGGTCAGATCCGGTGGTTTACACTATTGTAGTAATG
CTATCAAATTCGTACCAGACTTGAAAAAGATTCCAAAATTCCAAGATTTAACCTCAAAAG
ATGCCCTCTCACCTGAAACAATTCAAGCTAGTACCAATTTGGATTCAATCATTCATAATC
TTTCAAATAATTTATCAGAGGGTACTGAATACTTTAAGATGTTGGTAAACGTTTTCGCTA
CAGAGTTTCGAAATATCGCTAATCAACATTTAAAGAATTTCTATATCATCGTACCTGCCT
TGGCCTCAAATTACATCGATCATATGATCAATAGTAAAGATAAACTCTTTAAGAAATCAA
AAGCAATCGGTGCTGAAGCTTTATTATTCACTGATGATGGCTTTGCAATTGGTTTGGCTT
ATATCTTAAAATTATTAGATCAAAATAAAGATTTCGATTCTCTTCATTGGTTCGATCGTA
TTCAATTGAAATGTGAAACTGATCAAAAGAGAATGTTGGCTGAAGCAAATGCAAAAGGTA
TCAAAGAGGATCAAAATCATTTAGCAATTAAAAAGATTCAAAATTTCCAACAAGAATATG
AATTATTT