NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFJ178
Library AF
(Link to library)
Clone ID AFJ178
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16540-1
Original site URL
Representative seq. ID AFJ178P
(Link to Original site)
Representative DNA sequence 
>AFJ178 (AFJ178Q) /CSM/AF/AFJ1-D/AFJ178Q.Seq.d/
TTGGCCTACTGGTANTCAACTAAATTAAATAAATAATAAANTAAAAAAATAAAAAAAATG
AGGATAAATAATTTATTAGTATGTTTGGTTTTAGTTTTCTCAACATTATCAATTAGTAAT
GCAAATCCGGAACATGATAAATGGTGGAAACCTCCTCCTAGAAATTGTGATAGTTTGTCA
GAGGACCAATGTAAAGCACCAAATTCTGGTTGTNNGTATTTACCTTTTGTTTCATGTTGT
GGTACAAAGAANTNCTTTTGTGTAGAAGATAATGGAAATGGTTGTGGAAATGCTCCATTA
AGTTGTATGAAAGATTCAAAAACCGATGCAATTTATGAGATTTGGAGTTCATGTAGACCA
AATTCACCATTTTTATATGATTATCAAGTTAGAAATGAAACTTGTGATCAAAAGCTATGT
GAAGCAAGTGGAGGAGTTTGTGAATGGGTTGACCCAGTACCATGTATGGGTACATCATGT
TGTCCACGTTATCCAGTTTGNAAGGGTGGNGGNGGCGXXXXXXXXXXGTCCACATTGCCA
AAGACCAAATCCAGGCTCATTATGTCGTAATGTAACTTGTCCATATGGCTATGTTTGCAA
GGCAATTAATAATCTTCCAACATGTATTCGTAATCCATTACCACCAAACTACCCATGTAG
AGATTTACATTGCCCATCTGGATACTCTTGCGAAATCATCAATGATCTCCCATCATGCGT
AANAGAAACCCATCCAGGTCATTGTAAAACTTGTCATGATGTTAATTGTGGTTCATTGGA
TTGCGCAATGGTTCCTAATAAATGTCCAAGAGGTTCTCGTGACTGCTGTATTCAAATTCC
ATCGTGTCGTTTACCATCAAATTCTAATGATGATTATGATAATGATCCTAATGGATATGA
TGATAATGAAAATGAGGGTGATGGATTATCAATTCCATGTGGCCCAATTATCACTTGTAA
ATTAAATGAAATTTGTTTATTAGAACAATCACGTTGTGCCCCANTAT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
laywxstklnk**XKKIKKMRINNLLVCLVLVFSTLSISNANPEHDKWWKPPPRNCDSLS
EDQCKAPNSGCXYLPFVSCCGTKXXFCVEDNGNGCGNAPLSCMKDSKTDAIYEIWSSCRP
NSPFLYDYQVRNETCDQKLCEASGGVCEWVDPVPCMGTSCCPRYPVXKGGGG---

---PHCQRPNPGSLCRNVTCPYGYVCKAINNLPTCIRNPLPPNYPCRDLHCPSGYSCEII
NDLPSCVXETHPGHCKTCHDVNCGSLDCAMVPNKCPRGSRDCCIQIPSCRLPSNSNDDYD
NDPNGYDDNENEGDGLSIPCGPIITCKLNEICLLEQSRCAPX
Translated Amino Acid sequence (All Frames) 
Frame A:
laywxstklnk**XKKIKKMRINNLLVCLVLVFSTLSISNANPEHDKWWKPPPRNCDSLS
EDQCKAPNSGCXYLPFVSCCGTKXXFCVEDNGNGCGNAPLSCMKDSKTDAIYEIWSSCRP
NSPFLYDYQVRNETCDQKLCEASGGVCEWVDPVPCMGTSCCPRYPVXKGGGG---

---vhiakdqiqahyvvm*lvhmamfarqliifqhvfvihyhqtthveiyiahldtlaks
smishha*xkpiqvivklvmmlivvhwiaqwflinvqevlvtavfkfhrvvyhqilmmim
imilmdmmimkmrvmdyqfhvaqlslvn*mkfvy*nnhvvpxy

Frame B:
wptgxqln*innkxkk*kk*g*iiy*yvwf*fsqhyqlvmqirnminggnllleivivcq
rtnvkhqilvvxiyllfhvvvqrxxfv*kimemvvemlh*vv*kiqkpmqfmrfgvhvdq
ihhfymiiklemklviksyvkqveefvngltqyhvwvhhvvhviqfxrvxxa---

---stlpktksrlims*cnlsiwlclqgn**ssnmys*sittklpm*rftlpiwillrnh
q*spimrxrnpsrsl*nls*c*lwfiglrngs**mskrfs*llysnsivsftikf***l*
**s*wi****k*g*wiinsmwpnyhl*ik*nlfirtitlcpx

Frame C:
gllvxn*ik*iix*knkknedk*fismfgfsflniin**cksgt**mvetss*kl**fvr
gpm*stkfwlxvftfcfmlwykexllcrr*wkwlwkcsiklyerfknrcnl*dlefm*tk
ftifi*lss*k*nl*skam*skwrsl*mg*pstmygyimlstlsslxgwxx---

---PHCQRPNPGSLCRNVTCPYGYVCKAINNLPTCIRNPLPPNYPCRDLHCPSGYSCEII
NDLPSCVXETHPGHCKTCHDVNCGSLDCAMVPNKCPRGSRDCCIQIPSCRLPSNSNDDYD
NDPNGYDDNENEGDGLSIPCGPIITCKLNEICLLEQSRCAPX
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLE226 (SLE226Q) /CSM/SL/SLE2-B/SLE226Q.Seq.d/                        868   0.0
SFL363 (SFL363Q) /CSM/SF/SFL3-C/SFL363Q.Seq.d/                        868   0.0
SFK851 (SFK851Q) /CSM/SF/SFK8-C/SFK851Q.Seq.d/                        868   0.0
SFJ163 (SFJ163Q) /CSM/SF/SFJ1-C/SFJ163Q.Seq.d/                        868   0.0
SFE669 (SFE669Q) /CSM/SF/SFE6-C/SFE669Q.Seq.d/                        868   0.0
CHC584 (CHC584Q) /CSM/CH/CHC5-D/CHC584Q.Seq.d/                        868   0.0
CFG712 (CFG712Q) /CSM/CF/CFG7-A/CFG712Q.Seq.d/                        868   0.0
CFF677 (CFF677Q) /CSM/CF/CFF6-D/CFF677Q.Seq.d/                        868   0.0
CFF190 (CFF190Q) /CSM/CF/CFF1-D/CFF190Q.Seq.d/                        868   0.0
CFE212 (CFE212Q) /CSM/CF/CFE2-A/CFE212Q.Seq.d/                        868   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC111035|AC111035.8 Mus musculus chromosome 3, clone RP24-507N18, complete sequence.    40   0.022  6
CG730970|CG730970.1 1119131F03.y1 1119 - RescueMu Grid AA Zea mays genomic, genomic survey sequence.    30   0.025  4
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    32   0.12   17
CC262402|CC262402.1 CH261-72I18_Sp6.1 CH261 Gallus gallus genomic clone CH261-72I18, DNA sequence.    48   0.28   1
CB910401|CB910401.1 VVD177H03_378713 An expressed sequence tag database for abiotic stressed berries of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVD177H03 5, mRNA sequence.    38   0.35   2
AC117072|AC117072.3 Dictyostelium discoideum chromosome 2 map 3879572-4071762 strain AX4, complete sequence.    32   0.56   9
BQ798539|BQ798539.1 EST 708 Green Grape berries Lambda Zap II Library Vitis vinifera cDNA clone GB005C04 3', mRNA sequence.    38   0.73   2
BQ799355|BQ799355.1 EST 1524 Green Grape berries Lambda Zap II Library Vitis vinifera cDNA clone GT181G07 3', mRNA sequence.    38   0.86   2
CA817009|CA817009.1 CA12EI202IIIR_G06 CA12EI Vitis vinifera cDNA clone CA12EI202IIIR_G06 3', mRNA sequence.    38   0.88   2
CR450770|CR450770.1 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone.    38   0.93   4
dna update 2004. 9.24
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54QX2) RecName: Full=Probable spore coat protein DDB_G0283555;...   336   8e-98
(Q04503) RecName: Full=Prespore protein Dp87; Flags: Precursor;       120   1e-37
D13973_1(D13973|pid:none) Dictyostelium discoideum gene for Dp87...   117   2e-35
(P15269) RecName: Full=Spore coat protein SP70; AltName: Full=Pr...    94   8e-22
S07638(S07638;A60942;B60942) spore coat protein SP96 precursor -...    86   2e-15
(P14328) RecName: Full=Spore coat protein SP96;  &AC117075_49(AC...    86   2e-15
AF245486_1(AF245486|pid:none) Polysphondylium pallidum spore coa...    61   7e-08
AC117076_49(AC117076|pid:none) Dictyostelium discoideum chromoso...    41   4e-06
AC117076_50(AC117076|pid:none) Dictyostelium discoideum chromoso...    40   6e-06
S72639_1(S72639|pid:none) PspB=prespore-specific gene {5' region...    49   2e-04
protein update 2009. 4.23
PSORT 

 psg: 0.98  gvh: 0.81  alm: 0.40  top: 0.17  tms: 0.00  mit: 0.37  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 64.0 %: extracellular, including cell wall
	 12.0 %: endoplasmic reticulum
	  8.0 %: cytoplasmic
	  8.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: nuclear

>> prediction for AFJ178 is exc
5' end seq. ID AFJ178F
5' end seq. 
>AFJ178F.Seq
TTGGCCTACTGGTANTCAACTAAATTAAATAAATAATAAANTAAAAAAATAAAAAAAATG
AGGATAAATAATTTATTAGTATGTTTGGTTTTAGTTTTCTCAACATTATCAATTAGTAAT
GCAAATCCGGAACATGATAAATGGTGGAAACCTCCTCCTAGAAATTGTGATAGTTTGTCA
GAGGACCAATGTAAAGCACCAAATTCTGGTTGTNNGTATTTACCTTTTGTTTCATGTTGT
GGTACAAAGAANTNCTTTTGTGTAGAAGATAATGGAAATGGTTGTGGAAATGCTCCATTA
AGTTGTATGAAAGATTCAAAAACCGATGCAATTTATGAGATTTGGAGTTCATGTAGACCA
AATTCACCATTTTTATATGATTATCAAGTTAGAAATGAAACTTGTGATCAAAAGCTATGT
GAAGCAAGTGGAGGAGTTTGTGAATGGGTTGACCCAGTACCATGTATGGGTACATCATGT
TGTCCACGTTATCCAGTTTGNAAGGGTGGNGGNGGCG----------
Length of 5' end seq. 517
3' end seq. ID AFJ178Z
3' end seq. 
>AFJ178Z.Seq
----------GTCCACATTGCCAAAGACCAAATCCAGGCTCATTATGTCGTAATGTAACT
TGTCCATATGGCTATGTTTGCAAGGCAATTAATAATCTTCCAACATGTATTCGTAATCCA
TTACCACCAAACTACCCATGTAGAGATTTACATTGCCCATCTGGATACTCTTGCGAAATC
ATCAATGATCTCCCATCATGCGTAANAGAAACCCATCCAGGTCATTGTAAAACTTGTCAT
GATGTTAATTGTGGTTCATTGGATTGCGCAATGGTTCCTAATAAATGTCCAAGAGGTTCT
CGTGACTGCTGTATTCAAATTCCATCGTGTCGTTTACCATCAAATTCTAATGATGATTAT
GATAATGATCCTAATGGATATGATGATAATGAAAATGAGGGTGATGGATTATCAATTCCA
TGTGGCCCAATTATCACTTGTAAATTAAATGAAATTTGTTTATTAGAACAATCACGTTGT
GCCCCANTAT
Length of 3' end seq. 480
Connected seq. ID AFJ178P
Connected seq. 
>AFJ178P.Seq
TTGGCCTACTGGTANTCAACTAAATTAAATAAATAATAAANTAAAAAAATAAAAAAAATG
AGGATAAATAATTTATTAGTATGTTTGGTTTTAGTTTTCTCAACATTATCAATTAGTAAT
GCAAATCCGGAACATGATAAATGGTGGAAACCTCCTCCTAGAAATTGTGATAGTTTGTCA
GAGGACCAATGTAAAGCACCAAATTCTGGTTGTNNGTATTTACCTTTTGTTTCATGTTGT
GGTACAAAGAANTNCTTTTGTGTAGAAGATAATGGAAATGGTTGTGGAAATGCTCCATTA
AGTTGTATGAAAGATTCAAAAACCGATGCAATTTATGAGATTTGGAGTTCATGTAGACCA
AATTCACCATTTTTATATGATTATCAAGTTAGAAATGAAACTTGTGATCAAAAGCTATGT
GAAGCAAGTGGAGGAGTTTGTGAATGGGTTGACCCAGTACCATGTATGGGTACATCATGT
TGTCCACGTTATCCAGTTTGNAAGGGTGGNGGNGGCG----------GTCCACATTGCCA
AAGACCAAATCCAGGCTCATTATGTCGTAATGTAACTTGTCCATATGGCTATGTTTGCAA
GGCAATTAATAATCTTCCAACATGTATTCGTAATCCATTACCACCAAACTACCCATGTAG
AGATTTACATTGCCCATCTGGATACTCTTGCGAAATCATCAATGATCTCCCATCATGCGT
AANAGAAACCCATCCAGGTCATTGTAAAACTTGTCATGATGTTAATTGTGGTTCATTGGA
TTGCGCAATGGTTCCTAATAAATGTCCAAGAGGTTCTCGTGACTGCTGTATTCAAATTCC
ATCGTGTCGTTTACCATCAAATTCTAATGATGATTATGATAATGATCCTAATGGATATGA
TGATAATGAAAATGAGGGTGATGGATTATCAATTCCATGTGGCCCAATTATCACTTGTAA
ATTAAATGAAATTTGTTTATTAGAACAATCACGTTGTGCCCCANTAT
Length of connected seq. 997
Full length Seq ID -
Full length Seq. -
Length of full length seq. -