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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFJ171
Library AF
(Link to library)
Clone ID AFJ171
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U08808-1
Original site URL
Representative seq. ID AFJ171P
(Link to Original site)
Representative DNA sequence 
>AFJ171 (AFJ171Q) /CSM/AF/AFJ1-C/AFJ171Q.Seq.d/
AACCACATATAAATTAAATATTTTATCTCTTTAAATAATTTTTTTTTTTTTCTCTCCANC
ATCTTTTTTNTTTTTTTTNAAATATTAAAATTTTATNTTAAAAAAAAAAAAAAANGGTTT
TTAAAAAAAAAAXXXXXXXXXXTNTTTCAANATNTTTNAANGGNAAAGNTANGGNTTTTT
TTTTTGGGGGNTCAATTNCTTCAAAAAANGGNTNTTTANAANCCNTTTTTCCCCCCCCCC
TCCCCTTTTTTTTTTTTAAATAAAAGGNT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
nhi*IKYFISLNNFFFFLSXIFFXFFXILKFYXKKKKKXFLKKK---

---FQXXXXXKXXXFFLGXQXLQKXXXXXPFFPPPSPFFFLNKR
Translated Amino Acid sequence (All Frames) 
Frame A:
nhi*IKYFISLNNFFFFLSXIFFXFFXILKFYXKKKKKXFLKKK---

---xfxxfxxxxxgfffwgxnxfkkxxfxxxfspppplfff*ikg

Frame B:
ttyklnilsl*iiffffspxsfxffxky*nfxlkkkkxgf*kk---

---xsxxxxgkxxxfffggsixskxgxlxxxfppplpffffk*kx

Frame C:
phin*ifylfk*ffffslxhlfxffxnikilx*kkkkxvfkkk---

---FQXXXXXKXXXFFLGXQXLQKXXXXXPFFPPPSPFFFLNKR
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFJ171 (AFJ171Q) /CSM/AF/AFJ1-C/AFJ171Q.Seq.d/                         76   3e-13
VHA607 (VHA607Q) /CSM/VH/VHA6-A/VHA607Q.Seq.d/                         36   0.26
SSF219 (SSF219Q) /CSM/SS/SSF2-A/SSF219Q.Seq.d/                         34   1.0
FC-BI02 (FC-BI02Q) /CSM/FC/FC-BI/FC-BI02Q.Seq.d/                       34   1.0
CHI582 (CHI582Q) /CSM/CH/CHI5-D/CHI582Q.Seq.d/                         34   1.0
CHF747 (CHF747Q) /CSM/CH/CHF7-B/CHF747Q.Seq.d/                         34   1.0
VSI490 (VSI490Q) /CSM/VS/VSI4-D/VSI490Q.Seq.d/                         32   4.0
VSF861 (VSF861Q) /CSM/VS/VSF8-C/VSF861Q.Seq.d/                         32   4.0
VHN647 (VHN647Q) /CSM/VH/VHN6-B/VHN647Q.Seq.d/                         32   4.0
VHK810 (VHK810Q) /CSM/VH/VHK8-A/VHK810Q.Seq.d/                         32   4.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AX344618|AX344618.1 Sequence 43 from Patent WO0200927.             34   0.005  4
AC090491|AC090491.1 Mus musculus clone RP23-12K1, *** SEQUENCING IN PROGRESS ***, 43 unordered pieces.    48   0.015  2
AC139195|AC139195.2 Mus musculus chromosome UNK clone RP23-388P21, WORKING DRAFT SEQUENCE, 8 unordered pieces.    48   0.070  1
AC091332|AC091332.8 Mus musculus chromosome 17, clone RP23-12K1, complete sequence.    48   0.070  1
AC131974|AC131974.4 Mus musculus chromosome 17 clone RP23-243P19 map 17, *** SEQUENCING IN PROGRESS ***, 7 unordered pieces.    48   0.070  1
AC107649|AC107649.4 Mus musculus chromosome 17 clone RP23-128F3 map 17, *** SEQUENCING IN PROGRESS ***, 11 unordered pieces.    48   0.070  1
AZ365362|AZ365362.1 1M0112B06F Mouse 10kb plasmid UUGC1M library Mus musculus genomic clone UUGC1M0112B06 F, DNA sequence.    48   0.070  1
AB028611|AB028611.1 Arabidopsis thaliana genomic DNA, chromosome 3, P1 clone:MFE16.    32   0.46   5
AB164452|AB164452.1 Asphondylia diervillae mitochondrial gene for 12S ribosomal RNA, partial sequence, isolate: WeiFukInu62.    34   0.46   2
AB164451|AB164451.1 Asphondylia diervillae mitochondrial gene for 12S ribosomal RNA, partial sequence, isolate: WeiFuk56.    34   0.46   2
dna update 2004. 9.24
Homology vs Protein 

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3039690
Number of Hits to DB: 72,290,166
protein update 2009. 4.23
PSORT 

 psg: 0.98  gvh: 0.50  alm: 0.30  top: 1.00  tms: 0.07  mit: 0.42  mip: 0.00
 nuc: 0.08  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: cytoplasmic
	 28.0 %: nuclear
	 20.0 %: mitochondrial
	  8.0 %: plasma membrane
	  8.0 %: endoplasmic reticulum
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for AFJ171 is cyt
5' end seq. ID AFJ171F
5' end seq. 
>AFJ171F.Seq
AACCACATATAAATTAAATATTTTATCTCTTTAAATAATTTTTTTTTTTTTCTCTCCANC
ATCTTTTTTNTTTTTTTTNAAATATTAAAATTTTATNTTAAAAAAAAAAAAAAANGGTTT
TTAAAAAAAAAA----------
Length of 5' end seq. 132
3' end seq. ID AFJ171Z
3' end seq. 
>AFJ171Z.Seq
----------TNTTTCAANATNTTTNAANGGNAAAGNTANGGNTTTTTTTTTTGGGGGNT
CAATTNCTTCAAAAAANGGNTNTTTANAANCCNTTTTTCCCCCCCCCCTCCCCTTTTTTT
TTTTTAAATAAAAGGNT
Length of 3' end seq. 127
Connected seq. ID AFJ171P
Connected seq. 
>AFJ171P.Seq
AACCACATATAAATTAAATATTTTATCTCTTTAAATAATTTTTTTTTTTTTCTCTCCANC
ATCTTTTTTNTTTTTTTTNAAATATTAAAATTTTATNTTAAAAAAAAAAAAAAANGGTTT
TTAAAAAAAAAA----------TNTTTCAANATNTTTNAANGGNAAAGNTANGGNTTTTT
TTTTTGGGGGNTCAATTNCTTCAAAAAANGGNTNTTTANAANCCNTTTTTCCCCCCCCCC
TCCCCTTTTTTTTTTTTAAATAAAAGGNT
Length of connected seq. 259
Full length Seq ID -
Full length Seq. -
Length of full length seq. -