>AFJ167 (AFJ167Q) /CSM/AF/AFJ1-C/AFJ167Q.Seq.d/
AGTTGTTGATGGTTNAAAAACTGNATNNGNAAAATTCTATGCACCATGGTGTGGCCATTG
TAAAAAACTTGCACCAGCTTTTGAAATCCTTGCTGACACATTCGCACCAGTTTCCAACAA
AGTTGTAATTGCCAAAGTTGATTGTGACCAAGCTGACAACAAAGCTCTCTGTAGTAAATA
TGATGTTTCTGGTTATCCAACCCTCAAAATNTTTGACAAATCAACCACTGCCAAAGATTA
CAACGGNGCTAGATCAGTTGATGAATTATTAACTTATATCAATAATCACGCCAAAACCAA
CGTTAAAGTAAAGAAAGCACCATCAAATGTTGTTGATTTATCACCATCAAATTNTGATTC
AGTTGNTNTAGATAAATCAAAGAATGTTTTAGTTGAATTCTATGCACCATGGTGTGGTCA
TTGCAAAAAGTTAATGCCAGATTATGAAATCCTTGNTAACACXXXXXXXXXX

VVDGXKTXXXKFYAPWCGHCKKLAPAFEILADTFAPVSNKVVIAKVDCDQADNKALCSKY
DVSGYPTLKXFDKSTTAKDYNGARSVDELLTYINNHAKTNVKVKKAPSNVVDLSPSNXDS
VXXDKSKNVLVEFYAPWCGHCKKLMPDYEILXN---

Frame A:
sc*wxknxxxkilctmvwpl*ktctsf*npc*hirtsfqqscncqs*l*ps*qqssl**i
*cfwlsnpqnx*qinhcqrlqrx*is**iinlyq*srqnqr*skestikcc*fitikx*f
sxxr*ikecfs*ilctmvwslqkvnarl*npx*---


Frame B:
VVDGXKTXXXKFYAPWCGHCKKLAPAFEILADTFAPVSNKVVIAKVDCDQADNKALCSKY
DVSGYPTLKXFDKSTTAKDYNGARSVDELLTYINNHAKTNVKVKKAPSNVVDLSPSNXDS
VXXDKSKNVLVEFYAPWCGHCKKLMPDYEILXN---


Frame C:
llmvxklxxxnsmhhgvaivknlhqllksllthshqfptkl*lpklivtklttklsvvnm
mflviqpskxltnqplpkittxldqlmny*lisiitpkptlk*rkhhqmlliyhhqixiq
lx*inqrmf*lnsmhhgvviaks*cqimkslxt---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFJ167 (AFJ167Q) /CSM/AF/AFJ1-C/AFJ167Q.Seq.d/                        850   0.0
VSF163 (VSF163Q) /CSM/VS/VSF1-C/VSF163Q.Seq.d/                        842   0.0
VFO463 (VFO463Q) /CSM/VF/VFO4-C/VFO463Q.Seq.d/                        842   0.0
VFO241 (VFO241Q) /CSM/VF/VFO2-B/VFO241Q.Seq.d/                        842   0.0
VFN787 (VFN787Q) /CSM/VF/VFN7-D/VFN787Q.Seq.d/                        842   0.0
VFN233 (VFN233Q) /CSM/VF/VFN2-B/VFN233Q.Seq.d/                        842   0.0
VFM578 (VFM578Q) /CSM/VF/VFM5-D/VFM578Q.Seq.d/                        842   0.0
VFM537 (VFM537Q) /CSM/VF/VFM5-B/VFM537Q.Seq.d/                        842   0.0
VFM308 (VFM308Q) /CSM/VF/VFM3-A/VFM308Q.Seq.d/                        842   0.0
VFL481 (VFL481Q) /CSM/VF/VFL4-D/VFL481Q.Seq.d/                        842   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF019112|AF019112.1 Dictyostelium discoideum protein disulfide isomerase (dis) mRNA, complete cds.   842   0.0    3
AC116979|AC116979.3 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.   426   0.0    2
CN640516|CN640516.1 271E09_555565 Douglas-fir cDNA library PmIFG_73-6 Pseudotsuga menziesii var. menziesii cDNA clone Df271-E09 5, mRNA sequence.    66   2e-18  2
BQ695627|BQ695627.1 NXPV_030_G06_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda cDNA clone NXPV_030_G06 5', mRNA sequence.    54   9e-18  3
CV033767|CV033767.1 RTNACL1_36_B08.g1_A029 Roots plus added NaCl Pinus taeda cDNA clone RTNACL1_36_B08_A029 5', mRNA sequence.    54   2e-17  3
CO366743|CO366743.1 RTK1_29_F12.g1_A029 Roots minus potassium Pinus taeda cDNA clone RTK1_29_F12_A029 5', mRNA sequence.    54   2e-17  3
BX252003|BX252003.1 Pinus pinaster xylem EST, clone PP058D07.      54   2e-16  3
CF666748|CF666748.1 RTCNT1_25_H08.g1_A029 Root control Pinus taeda cDNA clone RTCNT1_25_H08_A029 5', mRNA sequence.    52   9e-16  3
AL663807|AL663807.1 Ciona intestinalis; larval mRNA; EST 3PRIM end of clone 020ZG06 of  library directional larval cDNA library from Ciona intestinalis  (Ciona).    82   1e-15  2
BW082456|BW082456.1 Ciona intestinalis cDNA, clone:rcieg088k11, 3' end, single read.    82   1e-15  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q86IA3) RecName: Full=Protein disulfide-isomerase 1;          S...   299   2e-80
AF019112_1(AF019112|pid:none) Dictyostelium discoideum protein d...   299   2e-80
EF086125_1(EF086125|pid:none) Picea sitchensis clone WS0285_I21 ...   168   6e-41
AB182629_1(AB182629|pid:none) Glycine max Gm pdis-1a mRNA for pr...   167   1e-40
AC087425_4(AC087425|pid:none) Oryza sativa (japonica cultivar-gr...   166   3e-40
AY570240_1(AY570240|pid:none) Brassica napus var. napus clone BN...   164   1e-39
DQ222488_1(DQ222488|pid:none) Solanum tuberosum clone 098B03 dis...   163   1e-39
AY739289_1(AY739289|pid:none) Zea mays protein disulfide isomera...   162   3e-39
AY739288_1(AY739288|pid:none) Zea mays protein disulfide isomera...   162   4e-39
EU966127_1(EU966127|pid:none) Zea mays clone 291908 PDIL2-2 - Ze...   162   4e-39

 psg: 0.82  gvh: 0.44  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.15  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: cytoplasmic
	 20.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for AFJ167 is cyt

>AFJ167F.Seq
AGTTGTTGATGGTTNAAAAACTGNATNNGNAAAATTCTATGCACCATGGTGTGGCCATTG
TAAAAAACTTGCACCAGCTTTTGAAATCCTTGCTGACACATTCGCACCAGTTTCCAACAA
AGTTGTAATTGCCAAAGTTGATTGTGACCAAGCTGACAACAAAGCTCTCTGTAGTAAATA
TGATGTTTCTGGTTATCCAACCCTCAAAATNTTTGACAAATCAACCACTGCCAAAGATTA
CAACGGNGCTAGATCAGTTGATGAATTATTAACTTATATCAATAATCACGCCAAAACCAA
CGTTAAAGTAAAGAAAGCACCATCAAATGTTGTTGATTTATCACCATCAAATTNTGATTC
AGTTGNTNTAGATAAATCAAAGAATGTTTTAGTTGAATTCTATGCACCATGGTGTGGTCA
TTGCAAAAAGTTAATGCCAGATTATGAAATCCTTGNTAACAC----------