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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHD517
Library SH
(Link to library)
Clone ID SHD517
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16507-1
Original site URL
Representative seq. ID SHD517P
(Link to Original site)
Representative DNA sequence 
>SHD517 (SHD517Q) /CSM/SH/SHD5-A/SHD517Q.Seq.d/
AAAAAAAAAAGCCACACACACATAAAAATGGTAATAAAAAAATTATTTATATTAATTTTT
TGTTTGTTTTTAATTATCAATGAAATTAATGGAAAAAAAACAAAAATAAATGATATTAAA
AAATCAAAACCAAAACTATCATCAACATTATTAAATGTTCATATTGTTGCTCATACTCAT
GATGATGTTGGTTGGTTAAAAACAGTTGATGAATATTATTATGGATCAAATATGTCAATT
GCATTTGCAGGTGTTCAATATACTTTGGATACAGCCATTACCTGTTTATTGGCAAATCCA
NAAANAAAGTTTATTTATGTTGAAATTGCATTTTTCCAAANATGGNGGGATGAACAAAGT
ACAACAATGCAAAATATTGTTAAGGGTTTGGTTGAAAGTGGTCAATTGGAATTCATAAAT
GGNGGGTATTGTATGAATGATGAGGCTACTACCTACTAXXXXXXXXXXGGTTCAACCAAC
CAGTGGTAATTATGTACCTGTGAATGCTATTGCCTACATTCAAGATCCTAATCAATCATT
ACAATTCACCATTGTCACTGATAGAAGTCGTGGATGTGCATCGTTAAGAGATGGTCAATT
GGATATGATGATGCATCGTCGTACTTTGAAAGATGATGGTCGTGGTGTTGGTCAACCAAT
GAATGAGTCAACTCAAATCGTTACAACTAGTAAATTGATTTTCCATGATATTTCAAGTTA
TGCTCAATCTCATTATCGTCCTGCTGCACTCTCTTTATCACATCCATTACTCCCAATGTT
TACAACCACTCAACAATCTTCAAACGATTGGAATTCTCAATATCAAGGTGTTTACTCACC
ATTGACTTCAGCCTCACCTTTACCAAATGGTTTAAAGATTCAAACTCTACAATGGTTAGA
TAATCAAGATAATACCATACTTTTACGTATTGAAAATATTTATCAAATTGATGGTCAAGA
TTCACAAGATCCACAAACTATCACTTTAGATCTTTCAACTATCTTTTCAACAATTACAAT
CACTTCTGCAACTGAAATGAATTTAACTGGTGTTCAAAAATTATCAAATTTATCAAGATT
AAAATGGAAAACTGTTGATGGTAAAAACTATGATCATAAATCATCATCATCAACTAAAGA
AGATTCTTCAAAGGTTTTGTTTTTACTTTTTCTCCAATGCAAATCAGAACTTT
sequence update 2002.10.25
Translated Amino Acid sequence 
KKKSHTHIKMVIKKLFILIFCLFLIINEINGKKTKINDIKKSKPKLSSTLLNVHIVAHTH
DDVGWLKTVDEYYYGSNMSIAFAGVQYTLDTAITCLLANPXXKFIYVEIAFFQXWXDEQS
TTMQNIVKGLVESGQLEFINGGYCMNDEATTY---

---VQPTSGNYVPVNAIAYIQDPNQSLQFTIVTDRSRGCASLRDGQLDMMMHRRTLKDDG
RGVGQPMNESTQIVTTSKLIFHDISSYAQSHYRPAALSLSHPLLPMFTTTQQSSNDWNSQ
YQGVYSPLTSASPLPNGLKIQTLQWLDNQDNTILLRIENIYQIDGQDSQDPQTITLDLST
IFSTITITSATEMNLTGVQKLSNLSRLKWKTVDGKNYDHKSSSSTKEDSSKVLFLLFLQC
KSEL
Translated Amino Acid sequence (All Frames) 
Frame A:
KKKSHTHIKMVIKKLFILIFCLFLIINEINGKKTKINDIKKSKPKLSSTLLNVHIVAHTH
DDVGWLKTVDEYYYGSNMSIAFAGVQYTLDTAITCLLANPXXKFIYVEIAFFQXWXDEQS
TTMQNIVKGLVESGQLEFINGGYCMNDEATTY---

---gstnqw*lctcecyclhsrs*siitihhch**kswmcivkrwsigyddassyfer*w
swcwstne*vnsnryn**idfp*yfklcsislsscctlfitsitpnvynhstifkrlefs
isrclltidfsltftkwfkdsnstmvr*sr*yhtfty*kylsn*wsrftrstnyhfrsfn
ylfnnynhfcn*nefnwcskiikfikikmenc*w*kl*s*iiiin*rrffkgfvftfspm
qirt

Frame B:
kkkatht*kw**knyly*ffvcf*lsmklmekkqk*milknqnqnyhqhy*mfilllilm
mmlvg*kqlmniimdqicqlhlqvfnilwiqplpvywqixkxslfmlklhfskxggmnkv
qqckillrvwlkvvnwns*mxgiv*mmrllpt---

---VQPTSGNYVPVNAIAYIQDPNQSLQFTIVTDRSRGCASLRDGQLDMMMHRRTLKDDG
RGVGQPMNESTQIVTTSKLIFHDISSYAQSHYRPAALSLSHPLLPMFTTTQQSSNDWNSQ
YQGVYSPLTSASPLPNGLKIQTLQWLDNQDNTILLRIENIYQIDGQDSQDPQTITLDLST
IFSTITITSATEMNLTGVQKLSNLSRLKWKTVDGKNYDHKSSSSTKEDSSKVLFLLFLQC
KSEL

Frame C:
kkkphthkngnkkiiyinflfvfnyq*n*wkknknk*y*kiktktiiniikcsyccsys*
*cwlvkns**illwikyvncicrcsiyfgyshylfigksxxkvylc*ncifpxmxg*tky
nnakyc*gfg*kwsigihkwxvlye**gyyll---

---fnqpvvimyl*mllptfkilinhynsplslievvdvhr*emvnwi**civvl*kmmv
vvlvnq*msqlkslqlvn*fsmifqvmlnliivllhslyhihysqclqplnnlqtigiln
ikvfthh*lqphlyqmv*rfklyng*iikiipyfyvlkifiklmvkihkihklsl*ifql
sfqqlqsllqlk*i*lvfknyqiyqd*ngkllmvktmiinhhhqlkkilqrfcfyffsna
nqnf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHD517 (SHD517Q) /CSM/SH/SHD5-A/SHD517Q.Seq.d/                       2008   0.0
VHQ167 (VHQ167Q) /CSM/VH/VHQ1-C/VHQ167Q.Seq.d/                       1437   0.0
VHP443 (VHP443Q) /CSM/VH/VHP4-B/VHP443Q.Seq.d/                       1437   0.0
VHO643 (VHO643Q) /CSM/VH/VHO6-B/VHO643Q.Seq.d/                       1437   0.0
VHK686 (VHK686Q) /CSM/VH/VHK6-D/VHK686Q.Seq.d/                       1437   0.0
VHI312 (VHI312Q) /CSM/VH/VHI3-A/VHI312Q.Seq.d/                       1437   0.0
VHI153 (VHI153Q) /CSM/VH/VHI1-C/VHI153Q.Seq.d/                       1437   0.0
VHH791 (VHH791Q) /CSM/VH/VHH7-D/VHH791Q.Seq.d/                       1437   0.0
VHE461 (VHE461Q) /CSM/VH/VHE4-C/VHE461Q.Seq.d/                       1437   0.0
VHE264 (VHE264Q) /CSM/VH/VHE2-C/VHE264Q.Seq.d/                       1437   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M82822|M82822.1 Dictyostelium discoideum alpha-mannosidase (manA) gene, complete cds.  1324   0.0    3
CK149800|CK149800.1 CsmgEST00336 Culicoides sonorensis female serum-fed midgut cDNA Library Culicoides sonorensis cDNA, mRNA sequence.   117   8e-24  3
BW354577|BW354577.1 Ciona intestinalis cDNA, clone:cima804e24, 5'end, single read.    66   3e-12  3
CV682907|CV682907.1 service43_H06_sjs2-16-2B11-T3 SJS Schistosoma japonicum cDNA, mRNA sequence.    74   3e-12  2
CV688215|CV688215.1 sjs2-16-2_G01_sjs2-16-2G01-T3 SJS Schistosoma japonicum cDNA, mRNA sequence.    74   4e-12  2
CV682854|CV682854.1 service30_G09_sjs2-14-10C09-T3 SJS Schistosoma japonicum cDNA, mRNA sequence.    74   4e-12  2
BU798369|BU798369.1 SJF2ADB01 SJF Schistosoma japonicum cDNA, mRNA sequence.    74   4e-12  2
BU798139|BU798139.1 SJF2AAF09 SJF Schistosoma japonicum cDNA, mRNA sequence.    74   4e-12  2
BU804021|BU804021.1 SJFBFG06 SJF Schistosoma japonicum cDNA, mRNA sequence.    74   4e-12  2
CB073433|CB073433.1 taa32h01.y1 Hydra EST -II Hydra magnipapillata cDNA 5' similar to TR:Q93093 Q93093 TRUNCATED LYSOSOMAL ACID ALPHA-MANNOSIDASE ;, mRNA sequence.    52   1e-10  3
dna update 2006. 2. 3
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P34098) RecName: Full=Lysosomal alpha-mannosidase;          Sho...   367   e-102
M82822_1(M82822|pid:none) Dictyostelium discoideum alpha-mannosi...   365   2e-99
(Q29451) RecName: Full=Lysosomal alpha-mannosidase;          Sho...   137   9e-31
AK291572_1(AK291572|pid:none) Homo sapiens cDNA FLJ76867 complet...   134   6e-30
U05572_1(U05572|pid:none) Human lysosomal alpha-mannosidase (MAN...   134   6e-30
AE014134_1973(AE014134|pid:none) Drosophila melanogaster chromos...   134   6e-30
AB209921_1(AB209921|pid:none) Homo sapiens mRNA for mannosidase,...   134   6e-30
AY036155_1(AY036155|pid:none) Cavia porcellus lysosomal alpha-ma...   133   1e-29
(Q8VHC8) RecName: Full=Lysosomal alpha-mannosidase;          Sho...   133   1e-29
JC2200(JC2200)alpha-mannosidase (EC 3.2.1.24) precursor - human       132   2e-29
protein update 2009. 4.16
PSORT 

 psg: 0.77  gvh: 0.62  alm: 0.17  top: 0.20  tms: 0.07  mit: 0.31  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 24.0 %: endoplasmic reticulum
	 20.0 %: cytoplasmic
	 16.0 %: nuclear
	 16.0 %: mitochondrial
	 12.0 %: Golgi
	  4.0 %: extracellular, including cell wall
	  4.0 %: peroxisomal
	  4.0 %: plasma membrane

>> prediction for SHD517 is end
5' end seq. ID SHD517F
5' end seq. 
>SHD517F.Seq
AAAAAAAAAAGCCACACACACATAAAAATGGTAATAAAAAAATTATTTATATTAATTTTT
TGTTTGTTTTTAATTATCAATGAAATTAATGGAAAAAAAACAAAAATAAATGATATTAAA
AAATCAAAACCAAAACTATCATCAACATTATTAAATGTTCATATTGTTGCTCATACTCAT
GATGATGTTGGTTGGTTAAAAACAGTTGATGAATATTATTATGGATCAAATATGTCAATT
GCATTTGCAGGTGTTCAATATACTTTGGATACAGCCATTACCTGTTTATTGGCAAATCCA
NAAANAAAGTTTATTTATGTTGAAATTGCATTTTTCCAAANATGGNGGGATGAACAAAGT
ACAACAATGCAAAATATTGTTAAGGGTTTGGTTGAAAGTGGTCAATTGGAATTCATAAAT
GGNGGGTATTGTATGAATGATGAGGCTACTACCTACTANNNNNNNNNN
Length of 5' end seq. 468
3' end seq. ID SHD517Z
3' end seq. 
>SHD517Z.Seq
NNNNNNNNNNGGTTCAACCAACCAGTGGTAATTATGTACCTGTGAATGCTATTGCCTACA
TTCAAGATCCTAATCAATCATTACAATTCACCATTGTCACTGATAGAAGTCGTGGATGTG
CATCGTTAAGAGATGGTCAATTGGATATGATGATGCATCGTCGTACTTTGAAAGATGATG
GTCGTGGTGTTGGTCAACCAATGAATGAGTCAACTCAAATCGTTACAACTAGTAAATTGA
TTTTCCATGATATTTCAAGTTATGCTCAATCTCATTATCGTCCTGCTGCACTCTCTTTAT
CACATCCATTACTCCCAATGTTTACAACCACTCAACAATCTTCAAACGATTGGAATTCTC
AATATCAAGGTGTTTACTCACCATTGACTTCAGCCTCACCTTTACCAAATGGTTTAAAGA
TTCAAACTCTACAATGGTTAGATAATCAAGATAATACCATACTTTTACGTATTGAAAATA
TTTATCAAATTGATGGTCAAGATTCACAAGATCCACAAACTATCACTTTAGATCTTTCAA
CTATCTTTTCAACAATTACAATCACTTCTGCAACTGAAATGAATTTAACTGGTGTTCAAA
AATTATCAAATTTATCAAGATTAAAATGGAAAACTGTTGATGGTAAAAACTATGATCATA
AATCATCATCATCAACTAAAGAAGATTCTTCAAAGGTTTTGTTTTTACTTTTTCTCCAAT
GCAAATCAGAACTTT
Length of 3' end seq. 735
Connected seq. ID SHD517P
Connected seq. 
>SHD517P.Seq
AAAAAAAAAAGCCACACACACATAAAAATGGTAATAAAAAAATTATTTATATTAATTTTT
TGTTTGTTTTTAATTATCAATGAAATTAATGGAAAAAAAACAAAAATAAATGATATTAAA
AAATCAAAACCAAAACTATCATCAACATTATTAAATGTTCATATTGTTGCTCATACTCAT
GATGATGTTGGTTGGTTAAAAACAGTTGATGAATATTATTATGGATCAAATATGTCAATT
GCATTTGCAGGTGTTCAATATACTTTGGATACAGCCATTACCTGTTTATTGGCAAATCCA
NAAANAAAGTTTATTTATGTTGAAATTGCATTTTTCCAAANATGGNGGGATGAACAAAGT
ACAACAATGCAAAATATTGTTAAGGGTTTGGTTGAAAGTGGTCAATTGGAATTCATAAAT
GGNGGGTATTGTATGAATGATGAGGCTACTACCTACTA----------GGTTCAACCAAC
CAGTGGTAATTATGTACCTGTGAATGCTATTGCCTACATTCAAGATCCTAATCAATCATT
ACAATTCACCATTGTCACTGATAGAAGTCGTGGATGTGCATCGTTAAGAGATGGTCAATT
GGATATGATGATGCATCGTCGTACTTTGAAAGATGATGGTCGTGGTGTTGGTCAACCAAT
GAATGAGTCAACTCAAATCGTTACAACTAGTAAATTGATTTTCCATGATATTTCAAGTTA
TGCTCAATCTCATTATCGTCCTGCTGCACTCTCTTTATCACATCCATTACTCCCAATGTT
TACAACCACTCAACAATCTTCAAACGATTGGAATTCTCAATATCAAGGTGTTTACTCACC
ATTGACTTCAGCCTCACCTTTACCAAATGGTTTAAAGATTCAAACTCTACAATGGTTAGA
TAATCAAGATAATACCATACTTTTACGTATTGAAAATATTTATCAAATTGATGGTCAAGA
TTCACAAGATCCACAAACTATCACTTTAGATCTTTCAACTATCTTTTCAACAATTACAAT
CACTTCTGCAACTGAAATGAATTTAACTGGTGTTCAAAAATTATCAAATTTATCAAGATT
AAAATGGAAAACTGTTGATGGTAAAAACTATGATCATAAATCATCATCATCAACTAAAGA
AGATTCTTCAAAGGTTTTGTTTTTACTTTTTCTCCAATGCAAATCAGAACTTT
Length of connected seq. 1183
Full length Seq ID -
Full length Seq. -
Length of full length seq. -