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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFJ144
Library AF
(Link to library)
Clone ID AFJ144
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15323-1
Original site URL
Representative seq. ID AFJ144E
(Link to Original site)
Representative DNA sequence 
>AFJ144 (AFJ144Q) /CSM/AF/AFJ1-B/AFJ144Q.Seq.d/
ATTTTATATTGCACACCATTATACTATATACTTTTTTTTTTAAAATGAATTCTAAATTTG
CTGATGAATTAATTGCAACTGCTAAAGCAATTGTAGCACCAGGTAAAGGTATTTTAGCAG
CAGATGAATCAACCAATACAATTGGAGCTCGTTTCAAAAAGATTAATTTAGAAAACAATG
AAGAGAACAGAAGAGCCTACAGAGAACTTTTAATTGGTACTGGTAATGGTGTTAACGAAT
TTATTGGTGGTATTATTTTATATGAAGAAACTCTTTACCAAAAGATGGCAGATGGTAGAT
TATTCACAGATGTTTTAAGAGAACAAAAGATTCACATTGGTATTAAAGTTGATAAGGGTG
TTGTACCAATTCCAGGTACCGATGGTGAAACCTCAACCCAAGGTTTAGATGGTCTTGCAG
AGCGTTGTAAGAAATACTATGAAGCAGGTGCACGTTTCGCAAAATGGAGAGCAGTCTTAA
AGATTGATCTTGCCAAGAATTGTCCATCCCAATTATCAATCACTGAAAATGCACACACAT
TAGCTCGTTATGCTGCAATTTGTCAAGAGAATGGTTTGGTCCCAATCGTCGAACCAGAAA
TCTTAATGGATGGTAATCACACTGTCGAGCAATCTGCTGAAGTCACTGAAAAAGTTTTAG
CTGCAGTTTTCAAAGCACTCAATGACCATCATATCTTATTAGAAGGTGCCTTATTAAAAC
CAAATATGGTCGTCAATGGTACCGACTGTCCAATCAAAGCCACCTCTGAACAAATTGGTA
AATTCACCGTCCGTACCCTTCAACGTACTGTTCCACCAGCTCTTACTGGTGTTGTTTTCT
TATCTGGTGGTCAAACTGAAATTGAAGCCACTGCAAACTTAAACGCTATGAATGTCTTAC
CAAATCGTCCATGGGCTCTCTCTTTCTCATACGGTCGTGCCTTACAGGCCTCTGTCATCT
CCACTTGGAAAGGTGAATCTGCCAACGTTGAAGCTGCTCGTAAAGTTTATTTACATCGTG
CCAAGTGTAACTCTCTTGCTCAACTCGGTAAATACACTGGTAGGAATCAACTGGTTCAGC
ATCGAATCAT
sequence update 2001. 6. 9
Translated Amino Acid sequence 
FILHTIILYTFFFKMNSKFADELIATAKAIVAPGKGILAADESTNTIGARFKKINLENNE
ENRRAYRELLIGTGNGVNEFIGGIILYEETLYQKMADGRLFTDVLREQKIHIGIKVDKGV
VPIPGTDGETSTQGLDGLAERCKKYYEAGARFAKWRAVLKIDLAKNCPSQLSITENAHTL
ARYAAICQENGLVPIVEPEILMDGNHTVEQSAEVTEKVLAAVFKALNDHHILLEGALLKP
NMVVNGTDCPIKATSEQIGKFTVRTLQRTVPPALTGVVFLSGGQTEIEATANLNAMNVLP
NRPWALSFSYGRALQASVISTWKGESANVEAARKVYLHRAKCNSLAQLGKYTGRNQLVQH
RI
Translated Amino Acid sequence (All Frames) 
Frame A:
ilyctplyyilfflk*ilnllmn*lqllkql*hqvkvf*qqmnqpiqlelvskrli*ktm
krteeptenf*lvlvmvltnllvvlfymkklftkrwqmvdysqmf*enkrftlvlklirv
lyqfqvpmvkpqpkv*mvlqsvvrntmkqvhvsqngeqs*rlilprivhpnyqslkmhth
*lvmlqfvkrmvwsqssnqks*wmvitlssnllkslkkf*lqfskhsmtiisy*kvpy*n
qiwssmvptvqskpplnklvnspsvpfnvlfhqlllvlfsylvvklklkplqt*tl*msy
qivhglslshtvvpyrplssplgkvnlptlkllvkfiyivpsvtlllnsvntlvginwfs
ies


Frame B:
fyiahhytiyfff*nef*ic**incnc*sncstr*ryfssr*inqynwssfqkd*frkq*
reqkslqrtfnwyw*wc*riywwyyfi*rnslpkdgrw*iihrcfkrtkdshwy*s**gc
ctnsryrw*nlnprfrwscral*eil*srctfrkmesslkd*scqelsipiinh*kcthi
sslccnlsrewfgpnrrtrnlngw*shcraic*sh*ksfscsfqstq*psylirrclikt
kygrqwyrlsnqshl*tnw*ihrpypstycstssywccfliwwsn*n*shcklkryeclt
kssmgslflirscltglchlhler*icqr*scs*slftscqv*lscstr*ihw*estgsa
snh


Frame C:
FILHTIILYTFFFKMNSKFADELIATAKAIVAPGKGILAADESTNTIGARFKKINLENNE
ENRRAYRELLIGTGNGVNEFIGGIILYEETLYQKMADGRLFTDVLREQKIHIGIKVDKGV
VPIPGTDGETSTQGLDGLAERCKKYYEAGARFAKWRAVLKIDLAKNCPSQLSITENAHTL
ARYAAICQENGLVPIVEPEILMDGNHTVEQSAEVTEKVLAAVFKALNDHHILLEGALLKP
NMVVNGTDCPIKATSEQIGKFTVRTLQRTVPPALTGVVFLSGGQTEIEATANLNAMNVLP
NRPWALSFSYGRALQASVISTWKGESANVEAARKVYLHRAKCNSLAQLGKYTGRNQLVQH
RI
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFJ144 (AFJ144Q) /CSM/AF/AFJ1-B/AFJ144Q.Seq.d/                       2101   0.0
AFN510 (AFN510Q) /CSM/AF/AFN5-A/AFN510Q.Seq.d/                       2074   0.0
VFK562 (VFK562Q) /CSM/VF/VFK5-C/VFK562Q.Seq.d/                       2068   0.0
VFI422 (VFI422Q) /CSM/VF/VFI4-A/VFI422Q.Seq.d/                       2068   0.0
CFF463 (CFF463Q) /CSM/CF/CFF4-C/CFF463Q.Seq.d/                       2068   0.0
CFE766 (CFE766Q) /CSM/CF/CFE7-C/CFE766Q.Seq.d/                       2056   0.0
CFJ531 (CFJ531Q) /CSM/CF/CFJ5-B/CFJ531Q.Seq.d/                       2054   0.0
SLB179 (SLB179Q) /CSM/SL/SLB1-D/SLB179Q.Seq.d/                       2052   0.0
CFC320 (CFC320Q) /CSM/CF/CFC3-A/CFC320Q.Seq.d/                       2050   0.0
CFI241 (CFI241Q) /CSM/CF/CFI2-B/CFI241Q.Seq.d/                       2048   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.  1939   0.0    2
AF247061|AF247061.1 Plasmodium vinckei fructose 1,6-bisphosphate aldolase gene, partial cds.    54   2e-26  5
BM167314|BM167314.1 EST569837 PyBS Plasmodium yoelii yoelii cDNA clone PYCOH24 5' end, mRNA sequence.    62   4e-24  5
AF247059|AF247059.1 Plasmodium yoelii YM fructose 1,6-bisphosphate aldolase gene, partial cds.    62   1e-23  5
AF247060|AF247060.1 Plasmodium chabaudi AS fructose 1,6-bisphosphate aldolase gene, partial cds.    54   2e-21  4
AF247062|AF247062.1 Plasmodium berghei QIMR fructose 1,6-bisphosphate aldolase gene, partial cds.    62   1e-19  5
BM162087|BM162087.1 EST564610 PyBS Plasmodium yoelii yoelii cDNA clone PYCKN08 5' end, mRNA sequence.    62   4e-16  4
M81793|M81793.1 Plasmodium berghei fructosebisphosphate aldolase (ALDO-2) gene, complete cds.    54   5e-16  5
BF297262|BF297262.1 049PbD12 Pb cDNA #17, Tommaso Pace, Marta Ponzi, and Clara Frontali Plasmodium berghei cDNA 5', mRNA sequence.    46   8e-12  4
AV525864|AV525864.1 Arabidopsis thaliana cDNA clone:APD31e12R, 5' end.    44   6e-11  4
dna update 2004. 7.29
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q86A67) RecName: Full=Fructose-bisphosphate aldolase;          ...   633   e-180
AL162506_11(AL162506|pid:none) Arabidopsis thaliana DNA chromoso...   393   e-108
AF247060_1(AF247060|pid:none) Plasmodium chabaudi AS fructose 1,...   393   e-108
AM910994_614(AM910994|pid:none) Plasmodium knowlesi strain H chr...   392   e-107
AY062566_1(AY062566|pid:none) Arabidopsis thaliana fructose-bisp...   392   e-107
AF308587_1(AF308587|pid:none) Fragaria x ananassa cytosolic aldo...   391   e-107
AF247063_1(AF247063|pid:none) Plasmodium vivax WDK fructose 1,6-...   391   e-107
DQ874472_1(DQ874472|pid:none) Plasmodium vivax strain A11 fructo...   391   e-107
EF144942_1(EF144942|pid:none) Populus trichocarpa clone WS0111_B...   389   e-106
EF134032_1(EF134032|pid:none) Perkinsus chesapeaki isolate Pch_c...   389   e-106
protein update 2009. 6.29
PSORT 

 psg: 0.97  gvh: 0.40  alm: 0.39  top: 0.50  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 24.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for AFJ144 is cyt
5' end seq. ID AFJ144F
5' end seq. 
>AFJ144F.Seq
ATTTTATATTGCACACCATTATACTATATACTTTTTTTTTTAAAATGAATTCTAAATTTG
CTGATGAATTAATTGCAACTGCTAAAGCAATTGTAGCACCAGGTAAAGGTATTTTAGCAG
CAGATGAATCAACCAATACAATTGGAGCTCGTTTCAAAAAGATTAATTTAGAAAACAATG
AAGAGAACAGAAGAGCCTACAGAGAACTTTTAATTGGTACTGGTAATGGTGTTAACGAAT
TTATTGGTGGTATTATTTTATATGAAGAAACTCTTTACCAAAAGATGGCAGATGGTAGAT
TATTCACAGATGTTTTAAGAGAACAAAAGATTCACATTGGTATTAAAGTTGATAAGGGTG
TTGTACCAATTCCAGGTACCGATGGTGAAACCTCAACCCAAGGTTTAGATGGTCTTGCAG
AGCGTTGTAAGAAATACTATGAAGCAGGTGCACGTTTCGCAAAATGGAGAGCAGTCTTAA
AGATTGATCTTGCCAAGAATTGTCCATCCCAATTATCAATCACTGAAAATGCACACACAT
TAGCTCGTTATGCTGCAATTTGTCA----------
Length of 5' end seq. 565
3' end seq. ID AFJ144Z
3' end seq. 
>AFJ144Z.Seq
----------TGGTCTTGCAGAGCGTTGTAAGAAATACTATGAAGCAGGTGCACGTTTCG
CAAAATGGAGAGCAGTCTTAAAGATTGATCTTGCCAAGAATTGTCCATCCCAATTATCAA
TCACTGAAAATGCACACACATTAGCTCGTTATGCTGCAATTTGTCAAGAGAATGGTTTGG
TCCCAATCGTCGAACCAGAAATCTTAATGGATGGTAATCACACTGTCGAGCAATCTGCTG
AAGTCACTGAAAAAGTTTTAGCTGCAGTTTTCAAAGCACTCAATGACCATCATATCTTAT
TAGAAGGTGCCTTATTAAAACCAAATATGGTCGTCAATGGTACCGACTGTCCAATCAAAG
CCACCTCTGAACAAATTGGTAAATTCACCGTCCGTACCCTTCAACGTACTGTTCCACCAG
CTCTTACTGGTGTTGTTTTCTTATCTGGTGGTCAAACTGAAATTGAAGCCACTGCAAACT
TAAACGCTATGAATGTCTTACCAAATCGTCCATGGGCTCTCTCTTTCTCATACGGTCGTG
CCTTACAGGCCTCTGTCATCTCCACTTGGAAAGGTGAATCTGCCAACGTTGAAGCTGCTC
GTAAAGTTTATTTACATCGTGCCAAGTGTAACTCTCTTGCTCAACTCGGTAAATACACTG
GTAGGAATCAACTGGTTCAGCATCGAATCAT
Length of 3' end seq. 681
Connected seq. ID AFJ144P
Connected seq. 
>AFJ144P.Seq
ATTTTATATTGCACACCATTATACTATATACTTTTTTTTTTAAAATGAATTCTAAATTTG
CTGATGAATTAATTGCAACTGCTAAAGCAATTGTAGCACCAGGTAAAGGTATTTTAGCAG
CAGATGAATCAACCAATACAATTGGAGCTCGTTTCAAAAAGATTAATTTAGAAAACAATG
AAGAGAACAGAAGAGCCTACAGAGAACTTTTAATTGGTACTGGTAATGGTGTTAACGAAT
TTATTGGTGGTATTATTTTATATGAAGAAACTCTTTACCAAAAGATGGCAGATGGTAGAT
TATTCACAGATGTTTTAAGAGAACAAAAGATTCACATTGGTATTAAAGTTGATAAGGGTG
TTGTACCAATTCCAGGTACCGATGGTGAAACCTCAACCCAAGGTTTAGATGGTCTTGCAG
AGCGTTGTAAGAAATACTATGAAGCAGGTGCACGTTTCGCAAAATGGAGAGCAGTCTTAA
AGATTGATCTTGCCAAGAATTGTCCATCCCAATTATCAATCACTGAAAATGCACACACAT
TAGCTCGTTATGCTGCAATTTGTCA----------TGGTCTTGCAGAGCGTTGTAAGAAA
TACTATGAAGCAGGTGCACGTTTCGCAAAATGGAGAGCAGTCTTAAAGATTGATCTTGCC
AAGAATTGTCCATCCCAATTATCAATCACTGAAAATGCACACACATTAGCTCGTTATGCT
GCAATTTGTCAAGAGAATGGTTTGGTCCCAATCGTCGAACCAGAAATCTTAATGGATGGT
AATCACACTGTCGAGCAATCTGCTGAAGTCACTGAAAAAGTTTTAGCTGCAGTTTTCAAA
GCACTCAATGACCATCATATCTTATTAGAAGGTGCCTTATTAAAACCAAATATGGTCGTC
AATGGTACCGACTGTCCAATCAAAGCCACCTCTGAACAAATTGGTAAATTCACCGTCCGT
ACCCTTCAACGTACTGTTCCACCAGCTCTTACTGGTGTTGTTTTCTTATCTGGTGGTCAA
ACTGAAATTGAAGCCACTGCAAACTTAAACGCTATGAATGTCTTACCAAATCGTCCATGG
GCTCTCTCTTTCTCATACGGTCGTGCCTTACAGGCCTCTGTCATCTCCACTTGGAAAGGT
GAATCTGCCAACGTTGAAGCTGCTCGTAAAGTTTATTTACATCGTGCCAAGTGTAACTCT
CTTGCTCAACTCGGTAAATACACTGGTAGGAATCAACTGGTTCAGCATCGAATCAT
Length of connected seq. 1246
Full length Seq ID AFJ144E
Full length Seq. 
>AFJ144E.Seq
ATTTTATATTGCACACCATTATACTATATACTTTTTTTTTTAAAATGAATTCTAAATTTG
CTGATGAATTAATTGCAACTGCTAAAGCAATTGTAGCACCAGGTAAAGGTATTTTAGCAG
CAGATGAATCAACCAATACAATTGGAGCTCGTTTCAAAAAGATTAATTTAGAAAACAATG
AAGAGAACAGAAGAGCCTACAGAGAACTTTTAATTGGTACTGGTAATGGTGTTAACGAAT
TTATTGGTGGTATTATTTTATATGAAGAAACTCTTTACCAAAAGATGGCAGATGGTAGAT
TATTCACAGATGTTTTAAGAGAACAAAAGATTCACATTGGTATTAAAGTTGATAAGGGTG
TTGTACCAATTCCAGGTACCGATGGTGAAACCTCAACCCAAGGTTTAGATGGTCTTGCAG
AGCGTTGTAAGAAATACTATGAAGCAGGTGCACGTTTCGCAAAATGGAGAGCAGTCTTAA
AGATTGATCTTGCCAAGAATTGTCCATCCCAATTATCAATCACTGAAAATGCACACACAT
TAGCTCGTTATGCTGCAATTTGTCAAGAGAATGGTTTGGTCCCAATCGTCGAACCAGAAA
TCTTAATGGATGGTAATCACACTGTCGAGCAATCTGCTGAAGTCACTGAAAAAGTTTTAG
CTGCAGTTTTCAAAGCACTCAATGACCATCATATCTTATTAGAAGGTGCCTTATTAAAAC
CAAATATGGTCGTCAATGGTACCGACTGTCCAATCAAAGCCACCTCTGAACAAATTGGTA
AATTCACCGTCCGTACCCTTCAACGTACTGTTCCACCAGCTCTTACTGGTGTTGTTTTCT
TATCTGGTGGTCAAACTGAAATTGAAGCCACTGCAAACTTAAACGCTATGAATGTCTTAC
CAAATCGTCCATGGGCTCTCTCTTTCTCATACGGTCGTGCCTTACAGGCCTCTGTCATCT
CCACTTGGAAAGGTGAATCTGCCAACGTTGAAGCTGCTCGTAAAGTTTATTTACATCGTG
CCAAGTGTAACTCTCTTGCTCAACTCGGTAAATACACTGGTAGGAATCAACTGGTTCAGC
ATCGAATCAT
Length of full length seq. 1090