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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHD280
Library SH
(Link to library)
Clone ID SHD280
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15687-1
Original site URL
Representative seq. ID SHD280P
(Link to Original site)
Representative DNA sequence 
>SHD280 (SHD280Q) /CSM/SH/SHD2-D/SHD280Q.Seq.d/
AACCCAATTTAGAAAATGGCCACATCCTATTCGGCTAATAATGGTCAAAATGAAGAATAT
ACATTTGATGCATTTGAAGATGGTTCATCTAAATTGGTTGGAGAGCAACAATTAACTAAA
CTTAATCAATTTGCAGTTGATTATGCACAACAAATTTGGAAGATAGAAGAAGCATTAGAT
GAAACATTAAATGAAGTATGGGATATAAATATAGATCCAGTTTCAATTTATAATAAACCT
CATGAACAATTAGATTTAATTGAATTGGTTAAAACTGATCATAAAATTTTCAATAAAGTA
ATATTAGTATTCTCTTCAATTTGTAATCAAACTAGAATTTTAAAAGAAACAGCAGAAAGT
AAATTTTATTCACCATTAACAGTATTTGGAGAGATTACAGGTGAATCATCAGAAGGTGAT
GTACAAATTGAAGTTGGTAAATTATTACCATTTATGATTGATTTATCAGCATTTGTAAAT
AGATGTTATTCATTAATTAGAAATATTATTAGTCAATTTGCATCGATCTATCAATCACAA
AAGAATATACATACACAATTCTTTAAGAATGTTCATTTACAAGCTGTCTATTATTCAATG
ATTGATATATTTTCAGTATTAATAAATTTAGATAGTATAATTACACAAAATACTGCTXXX
XXXXXXXCCAAGAATATCAAATACTTTAGAGAGAATAAAGACCAATTGAATAATATGTAC
CNTTCAGAGTTGGTGACAGAGTTGGAAAGAGACATTAGACAATTGGGTGTCTCCGAGACT
GGTTTAACCTTTTTAGATCATTTTAGACTTTTAATCACTCATATTGGTAATGCTATGGGT
TATATTAGATTGGTCAGATCCGGTGGTTTACACTATTGTAGTAATGCTATCAAATTCGTA
CCAGACTTGAAAAAGATTCCAAAATTCCAAGATTTAACCTCAAAAGATGCCCTCTCACCT
GAAACAATTCAAGCTAGTACCAATTTGGATTCAATCATTCATAATCTTTCAAATAATTTA
TCAGAGGGTACTGAATACTTTAAGATGTTGGTAAACGTTTTCGCTACAGAGTTTCGAAAT
ATCGCTAATCAACATTTAAAGAATTTCTATATCATCGTACCTGCCTTGGCCTCAAATTAC
ATCGATCATATGATCAATAGTAAAGATAAACTCTTTAAGAAATCAAAAGCAATCGGTGCT
GAAGCTTTATTATTCACTGATGATGGCTTTGCAATTGGTTTGGCTTATATCTTAAAATTA
TTAGATCAAAATAAAGATTTCGATTCTCTTCATTGGTTCGATCGTATTCAATTGAAATGT
GAAACTGATCAAAAGAGAATGTTGGCTGAAGCAAATGCAAAAGGTATCAAAGAGGATCAA
AATCATTTAGCAATTAAAAAGATTCAAAATTTCCAACAAGAATAGAATTATTTAAATATT
CTTTCNCGGGTTCAAGA
sequence update 2002.10.25
Translated Amino Acid sequence 
npi*KMATSYSANNGQNEEYTFDAFEDGSSKLVGEQQLTKLNQFAVDYAQQIWKIEEALD
ETLNEVWDINIDPVSIYNKPHEQLDLIELVKTDHKIFNKVILVFSSICNQTRILKETAES
KFYSPLTVFGEITGESSEGDVQIEVGKLLPFMIDLSAFVNRCYSLIRNIISQFASIYQSQ
KNIHTQFFKNVHLQAVYYSMIDIFSVLINLDSIITQNTA---

---KNIKYFRENKDQLNNMYXSELVTELERDIRQLGVSETGLTFLDHFRLLITHIGNAMG
YIRLVRSGGLHYCSNAIKFVPDLKKIPKFQDLTSKDALSPETIQASTNLDSIIHNLSNNL
SEGTEYFKMLVNVFATEFRNIANQHLKNFYIIVPALASNYIDHMINSKDKLFKKSKAIGA
EALLFTDDGFAIGLAYILKLLDQNKDFDSLHWFDRIQLKCETDQKRMLAEANAKGIKEDQ
NHLAIKKIQNFQQE*nylnilsrvq
Translated Amino Acid sequence (All Frames) 
Frame A:
npi*KMATSYSANNGQNEEYTFDAFEDGSSKLVGEQQLTKLNQFAVDYAQQIWKIEEALD
ETLNEVWDINIDPVSIYNKPHEQLDLIELVKTDHKIFNKVILVFSSICNQTRILKETAES
KFYSPLTVFGEITGESSEGDVQIEVGKLLPFMIDLSAFVNRCYSLIRNIISQFASIYQSQ
KNIHTQFFKNVHLQAVYYSMIDIFSVLINLDSIITQNTA---

---prisntleriktn*iictxqsw*qswketldnwvsprlv*pf*iildf*slilvmlw
vildwsdpvvytivvmlsnsyqt*krfqnski*pqkmpshlkqfklvpiwiqsfiifqii
yqrvlntlrcw*tfslqsfeislini*risissylpwpqitsii*sivkinslrnqkqsv
lklyyslmmalqlvwlis*ny*ikikisilfigsivfn*nvklikrecwlkqmqkvskri
kii*qlkrfkisnknrii*iffxgfk

Frame B:
tqfrkwphpirlimvkmknihlmhlkmvhlnwlesnn*lnlinlqlimhnkfgr*kkh*m
kh*mkygi*i*iqfqfiinlmnn*i*lnwlkliikfsik*y*yslqfviklef*kkqqkv
nfihh*qylerlqvnhqkvmyklklvnyyhl*liyqhl*idvih*leillvnlhrsinhk
riyihnslrmfiyklsiiq*liyfqy**i*iv*lhkil---

---qeyqil*re*rpie*yvpfrvgdrvgkrh*tigclrdwfnlfrsf*tfnhsyw*cyg
ly*igqirwftll**cyqirtrlekdskiprfnlkrcplt*nnss*yqfgfnhs*sfk*f
irgy*il*dvgkrfryrvskyr*stfkeflyhrtclglklhrsydq**r*tl*eiksnrc
*sfiih**wlcnwfglylkiirsk*rfrfsslvrsysiem*n*skenvg*skckryqrgs
ksfsn*kdskfptrielfkysfxgsr

Frame C:
pnlenghilfg**wsk*riyi*ci*rwfi*igwratin*t*sics*lcttnledrrsir*
nik*smgykyrssfnl**ts*tirfn*ig*n*s*nfq*snisilfnl*sn*nfkrnsrk*
ilftinsiwrdyr*iirr*ctn*sw*iitiyd*fisick*mlfin*kyy*sicidlsitk
eytytil*ecsftscllfnd*yifsinkfr*ynytkyc---

---KNIKYFRENKDQLNNMYXSELVTELERDIRQLGVSETGLTFLDHFRLLITHIGNAMG
YIRLVRSGGLHYCSNAIKFVPDLKKIPKFQDLTSKDALSPETIQASTNLDSIIHNLSNNL
SEGTEYFKMLVNVFATEFRNIANQHLKNFYIIVPALASNYIDHMINSKDKLFKKSKAIGA
EALLFTDDGFAIGLAYILKLLDQNKDFDSLHWFDRIQLKCETDQKRMLAEANAKGIKEDQ
NHLAIKKIQNFQQE*nylnilsrvq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHD280 (SHD280Q) /CSM/SH/SHD2-D/SHD280Q.Seq.d/                       2817   0.0
VHK424 (VHK424Q) /CSM/VH/VHK4-A/VHK424Q.Seq.d/                       1532   0.0
CHG465 (CHG465Q) /CSM/CH/CHG4-C/CHG465Q.Seq.d/                       1524   0.0
VHB619 (VHB619Q) /CSM/VH/VHB6-A/VHB619Q.Seq.d/                       1518   0.0
SHD671 (SHD671Q) /CSM/SH/SHD6-C/SHD671Q.Seq.d/                       1516   0.0
AHN345 (AHN345Q) /CSM/AH/AHN3-B/AHN345Q.Seq.d/                       1516   0.0
AHL641 (AHL641Q) /CSM/AH/AHL6-B/AHL641Q.Seq.d/                       1516   0.0
VHK538 (VHK538Q) /CSM/VH/VHK5-B/VHK538Q.Seq.d/                       1515   0.0
VHB821 (VHB821Q) /CSM/VH/VHB8-A/VHB821Q.Seq.d/                       1509   0.0
CHL807 (CHL807Q) /CSM/CH/CHL8-A/CHL807Q.Seq.d/                       1505   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BU746157|BU746157.1 CH3#002_C07T7 Canine heart normalized cDNA Library in pBluescriptCanis familiaris cDNA clone CH3#002_C07 5', mRNA sequence.    38   8e-04  3
BY464171|BY464171.1 Mus musculus 15 days pregnant adult female amnion cDNA, RIKEN full-length enriched library, clone:K630150M24, 3' end partial sequence.    42   0.028  2
AK113047|AK113047.1 Ciona intestinalis cDNA, clone:ciad002a21, full insert sequence.    48   0.034  2
AF063866|AF063866.1 Melanoplus sanguinipes entomopoxvirus, complete genome.    32   0.042  13
BX842643|BX842643.1 Mycoplasma mycoides subsp. mycoides SC genomic DNA, complete  sequence; segment 2/4.    34   0.054  16
BA000026|BA000026.2 Mycoplasma penetrans HF-2 DNA, complete genome.    36   0.076  24
BB822673|BB822673.1 Mus musculus mammary gland RCB-0526 Jyg-MC(A) cDNA, RIKEN full-length enriched library, clone:G830022O04, 3' end partial sequence.    32   0.13   4
CR450713|CR450713.2 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone.    38   0.13   8
CR044130|CR044130.1 Forward strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP186g09.    50   0.14   1
CR023465|CR023465.1 Forward strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP421e10.    50   0.14   1
dna update 2006. 1.30
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54R74) RecName: Full=UPF0681 protein;                               500   e-140
BC040936_1(BC040936|pid:none) Homo sapiens KIAA1033, mRNA (cDNA ...   247   8e-64
AB028956_1(AB028956|pid:none) Homo sapiens mRNA for KIAA1033 pro...   246   1e-63
BC143373_1(BC143373|pid:none) Homo sapiens KIAA1033, mRNA (cDNA ...   246   1e-63
BC020314_1(BC020314|pid:none) Mus musculus RIKEN cDNA A230046K03...   244   4e-63
(Q3UMB9) RecName: Full=UPF0681 protein KIAA1033;                      244   4e-63
AK161247_1(AK161247|pid:none) Mus musculus adult male testis cDN...   244   4e-63
AK145000_1(AK145000|pid:none) Mus musculus mammary gland RCB-052...   244   4e-63
BC110850_1(BC110850|pid:none) Homo sapiens KIAA1033, mRNA (cDNA ...   191   4e-47
AX882617_1(AX882617|pid:none) Sequence 17522 from Patent EP10746...   187   1e-45
protein update 2009. 4.16
PSORT 

 psg: 0.79  gvh: 0.25  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 40.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: vacuolar
	  4.0 %: plasma membrane

>> prediction for SHD280 is nuc
5' end seq. ID SHD280F
5' end seq. 
>SHD280F.Seq
AACCCAATTTAGAAAATGGCCACATCCTATTCGGCTAATAATGGTCAAAATGAAGAATAT
ACATTTGATGCATTTGAAGATGGTTCATCTAAATTGGTTGGAGAGCAACAATTAACTAAA
CTTAATCAATTTGCAGTTGATTATGCACAACAAATTTGGAAGATAGAAGAAGCATTAGAT
GAAACATTAAATGAAGTATGGGATATAAATATAGATCCAGTTTCAATTTATAATAAACCT
CATGAACAATTAGATTTAATTGAATTGGTTAAAACTGATCATAAAATTTTCAATAAAGTA
ATATTAGTATTCTCTTCAATTTGTAATCAAACTAGAATTTTAAAAGAAACAGCAGAAAGT
AAATTTTATTCACCATTAACAGTATTTGGAGAGATTACAGGTGAATCATCAGAAGGTGAT
GTACAAATTGAAGTTGGTAAATTATTACCATTTATGATTGATTTATCAGCATTTGTAAAT
AGATGTTATTCATTAATTAGAAATATTATTAGTCAATTTGCATCGATCTATCAATCACAA
AAGAATATACATACACAATTCTTTAAGAATGTTCATTTACAAGCTGTCTATTATTCAATG
ATTGATATATTTTCAGTATTAATAAATTTAGATAGTATAATTACACAAAATACTGCTNNN
NNNNNNN
Length of 5' end seq. 667
3' end seq. ID SHD280Z
3' end seq. 
>SHD280Z.Seq
NNNNNNNNNNCCAAGAATATCAAATACTTTAGAGAGAATAAAGACCAATTGAATAATATG
TACCNTTCAGAGTTGGTGACAGAGTTGGAAAGAGACATTAGACAATTGGGTGTCTCCGAG
ACTGGTTTAACCTTTTTAGATCATTTTAGACTTTTAATCACTCATATTGGTAATGCTATG
GGTTATATTAGATTGGTCAGATCCGGTGGTTTACACTATTGTAGTAATGCTATCAAATTC
GTACCAGACTTGAAAAAGATTCCAAAATTCCAAGATTTAACCTCAAAAGATGCCCTCTCA
CCTGAAACAATTCAAGCTAGTACCAATTTGGATTCAATCATTCATAATCTTTCAAATAAT
TTATCAGAGGGTACTGAATACTTTAAGATGTTGGTAAACGTTTTCGCTACAGAGTTTCGA
AATATCGCTAATCAACATTTAAAGAATTTCTATATCATCGTACCTGCCTTGGCCTCAAAT
TACATCGATCATATGATCAATAGTAAAGATAAACTCTTTAAGAAATCAAAAGCAATCGGT
GCTGAAGCTTTATTATTCACTGATGATGGCTTTGCAATTGGTTTGGCTTATATCTTAAAA
TTATTAGATCAAAATAAAGATTTCGATTCTCTTCATTGGTTCGATCGTATTCAATTGAAA
TGTGAAACTGATCAAAAGAGAATGTTGGCTGAAGCAAATGCAAAAGGTATCAAAGAGGAT
CAAAATCATTTAGCAATTAAAAAGATTCAAAATTTCCAACAAGAATAGAATTATTTAAAT
ATTCTTTCNCGGGTTCAAGA
Length of 3' end seq. 800
Connected seq. ID SHD280P
Connected seq. 
>SHD280P.Seq
AACCCAATTTAGAAAATGGCCACATCCTATTCGGCTAATAATGGTCAAAATGAAGAATAT
ACATTTGATGCATTTGAAGATGGTTCATCTAAATTGGTTGGAGAGCAACAATTAACTAAA
CTTAATCAATTTGCAGTTGATTATGCACAACAAATTTGGAAGATAGAAGAAGCATTAGAT
GAAACATTAAATGAAGTATGGGATATAAATATAGATCCAGTTTCAATTTATAATAAACCT
CATGAACAATTAGATTTAATTGAATTGGTTAAAACTGATCATAAAATTTTCAATAAAGTA
ATATTAGTATTCTCTTCAATTTGTAATCAAACTAGAATTTTAAAAGAAACAGCAGAAAGT
AAATTTTATTCACCATTAACAGTATTTGGAGAGATTACAGGTGAATCATCAGAAGGTGAT
GTACAAATTGAAGTTGGTAAATTATTACCATTTATGATTGATTTATCAGCATTTGTAAAT
AGATGTTATTCATTAATTAGAAATATTATTAGTCAATTTGCATCGATCTATCAATCACAA
AAGAATATACATACACAATTCTTTAAGAATGTTCATTTACAAGCTGTCTATTATTCAATG
ATTGATATATTTTCAGTATTAATAAATTTAGATAGTATAATTACACAAAATACTGCT---
-------CCAAGAATATCAAATACTTTAGAGAGAATAAAGACCAATTGAATAATATGTAC
CNTTCAGAGTTGGTGACAGAGTTGGAAAGAGACATTAGACAATTGGGTGTCTCCGAGACT
GGTTTAACCTTTTTAGATCATTTTAGACTTTTAATCACTCATATTGGTAATGCTATGGGT
TATATTAGATTGGTCAGATCCGGTGGTTTACACTATTGTAGTAATGCTATCAAATTCGTA
CCAGACTTGAAAAAGATTCCAAAATTCCAAGATTTAACCTCAAAAGATGCCCTCTCACCT
GAAACAATTCAAGCTAGTACCAATTTGGATTCAATCATTCATAATCTTTCAAATAATTTA
TCAGAGGGTACTGAATACTTTAAGATGTTGGTAAACGTTTTCGCTACAGAGTTTCGAAAT
ATCGCTAATCAACATTTAAAGAATTTCTATATCATCGTACCTGCCTTGGCCTCAAATTAC
ATCGATCATATGATCAATAGTAAAGATAAACTCTTTAAGAAATCAAAAGCAATCGGTGCT
GAAGCTTTATTATTCACTGATGATGGCTTTGCAATTGGTTTGGCTTATATCTTAAAATTA
TTAGATCAAAATAAAGATTTCGATTCTCTTCATTGGTTCGATCGTATTCAATTGAAATGT
GAAACTGATCAAAAGAGAATGTTGGCTGAAGCAAATGCAAAAGGTATCAAAGAGGATCAA
AATCATTTAGCAATTAAAAAGATTCAAAATTTCCAACAAGAATAGAATTATTTAAATATT
CTTTCNCGGGTTCAAGA
Length of connected seq. 1447
Full length Seq ID -
Full length Seq. -
Length of full length seq. -