>SHD256 (SHD256Q) /CSM/SH/SHD2-C/SHD256Q.Seq.d/
AAATTTTACAACAGTTATACCGCACAAAAAAAAAATAATATAGAATTAATAAAGAAAATT
AAANGTTTAAATAACAAATACAAATACATATCAATAATTAATAATAATAATAATAACAAT
AATAAAAATAATAATAATATTAGNGAAATCATAAATTTAAATTAAATATAATATTCAAAT
AATAAATAATAATTAATTAATTAAAAAAAAAAXXXXXXXXXX

KFYNSYTAQKKNNIELIKKIKXLNNKYKYISIINNNNNNNNKNNNNIXEIINLN*i*ysn
nk**lin*kk---

Frame A:
KFYNSYTAQKKNNIELIKKIKXLNNKYKYISIINNNNNNNNKNNNNIXEIINLN*i*ysn
nk**lin*kk---


Frame B:
nfttviphkkkii*n**rklxv*itntntyq*liiiiitiikiiiilxks*i*ikyniqi
innn*likkk---


Frame C:
ilqqlyrtkkk*yrinken*xfk*qiqihinn******q**k***y*xnhkfklniifk*
*iiin*lkk---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHG676 (VHG676Q) /CSM/VH/VHG6-D/VHG676Q.Seq.d/                         50   1e-05
SHF679 (SHF679Q) /CSM/SH/SHF6-D/SHF679Q.Seq.d/                         50   1e-05
SHD543 (SHD543Q) /CSM/SH/SHD5-B/SHD543Q.Seq.d/                         50   1e-05
SHD256 (SHD256Q) /CSM/SH/SHD2-C/SHD256Q.Seq.d/                         50   1e-05
AHB816 (AHB816Q) /CSM/AH/AHB8-A/AHB816Q.Seq.d/                         50   1e-05
AHR273 (AHR273Q) /CSM/AH/AHR2-D/AHR273Q.Seq.d/                         44   9e-04
SHG630 (SHG630Q) /CSM/SH/SHG6-B/SHG630Q.Seq.d/                         42   0.003

 ***** No hits found ******

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Hits to DB: 482,166
Number of Sequences: 52486302
Number of extensions: 482166
Number of successful extensions: 106707
Number of sequences better than 10.0: 0
length of query: 222
length of database: 56,935,314,241
effective HSP length: 22
effective length of query: 200
effective length of database: 55,780,615,597
effective search space: 11156123119400
effective search space used: 11156123119400
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)

ANTI-DNA BLAST search was processed by blast@nig.ac.jp, National Institute of Genetics, Japan.

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3039690
Number of Hits to DB: 63,494,469

 psg: 0.48  gvh: -0.04  alm: 0.64  top: 0.53  tms: 0.00  mit: 0.22  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: nuclear
	 32.0 %: cytoplasmic
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: plasma membrane

>> prediction for SHD256 is nuc

>SHD256F.Seq
AAATTTTACAACAGTTATACCGCACAAAAAAAAAATAATATAGAATTAATAAAGAAAATT
AAANGTTTAAATAACAAATACAAATACATATCAATAATTAATAATAATAATAATAACAAT
AATAAAAATAATAATAATATTAGNGAAATCATAAATTTAAATTAAATATAATATTCAAAT
AATAAATAATAATTAATTAATTAAAAAAAAAANNNNNNNNNN