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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFJ136
Library AF
(Link to library)
Clone ID AFJ136
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16317-1
Original site URL
Representative seq. ID AFJ136P
(Link to Original site)
Representative DNA sequence 
>AFJ136 (AFJ136Q) /CSM/AF/AFJ1-B/AFJ136Q.Seq.d/
ACTGTTGGCCTACTGGTATCTATGCTATCCACACACCCAAAGTATTCATATTAATGGCAG
AAATAACAATATCAATTGAAAATGAAAATTTCATTCAAGTAAATTTACAACCAGATCAAA
CAGTTGAAGATCTAAAACGTAGAGTAGAATTTGAAACCACAATTTTAGTGAACAATCAAG
TTTTAACACTTGATGGAAAAGNNTTAGATAATGAAAAGAAATTATCAGACTATTCAATTA
AAGGTGGNGATTTCTTATTGATAACAAAAAATGTTTTAAGAGCACCACAACAAAGATCAC
AACAACCCCAACAACCACAACAACCACAACAACCACAXXXXXXXXXXAACCACCTACCAC
TACCACTACCACTACTTCACCCCAAACTCCAACTAATGCCACTTCTCCTGTAAATAATAA
TAATAATAATAATAACAACAACAACAACACTAGACCTCCAATTAATTCACCACCAGTTGC
AAAGACACCGCAACCAACACCACAACCAACTACAAATCAATATGCTTCCGAATCCAATAT
CCAAACCCTTATTGGACTTGGTTGTTCAAGAGATAAGGCAATTAGACTTTTACTCAAGCT
AAAGGCAATG
sequence update 2001. 6. 1
Translated Amino Acid sequence 
CWPTGIYAIHTPKVFILMAEITISIENENFIQVNLQPDQTVEDLKRRVEFETTILVNNQV
LTLDGKXLDNEKKLSDYSIKGGDFLLITKNVLRAPQQRSQQPQQPQQPQQP---

---PPTTTTTTTSPQTPTNATSPVNNNNNNNNNNNNTRPPINSPPVAKTPQPTPQPTTNQ
YASESNIQTLIGLGCSRDKAIRLLLKLKAM
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllvsmlsthpkysy*wqk*qyqlkmkisfk*iynqikqlki*nve*nlkpqf**tik
f*hlmekx*imkrnyqtiqlkvxisy**qkmf*ehhnkdhnnpnnhnnhnnh---

---nhlplplpllhpklqlmplll*iiiiiiitttttldlqlihhqlqrhrnqhhnqlqi
nmlpnpiskplldlvvqeirqldfyss*rq

Frame B:
llaywylcyphtqsihingrnnnin*k*kfhsskfttrsns*rskt*sri*nhnfseqss
fnt*wkxxr**keiirlfn*rwxflidnkkcfkstttkitttpttttttttt---

---ttyhyhyhyftpnsn*chfsck*******qqqqh*tsn*fttsckdtatntttnyks
icfriqypnpywtwlfkr*gn*tftqakgn

Frame C:
CWPTGIYAIHTPKVFILMAEITISIENENFIQVNLQPDQTVEDLKRRVEFETTILVNNQV
LTLDGKXLDNEKKLSDYSIKGGDFLLITKNVLRAPQQRSQQPQQPQQPQQP---

---PPTTTTTTTSPQTPTNATSPVNNNNNNNNNNNNTRPPINSPPVAKTPQPTPQPTTNQ
YASESNIQTLIGLGCSRDKAIRLLLKLKAM
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFJ136 (AFJ136Q) /CSM/AF/AFJ1-B/AFJ136Q.Seq.d/                        553   e-156
VFE249 (VFE249Q) /CSM/VF/VFE2-C/VFE249Q.Seq.d/                        505   e-142
VFE196 (VFE196Q) /CSM/VF/VFE1-D/VFE196Q.Seq.d/                        505   e-142
SLC513 (SLC513Q) /CSM/SL/SLC5-A/SLC513Q.Seq.d/                        505   e-142
SFL472 (SFL472Q) /CSM/SF/SFL4-C/SFL472Q.Seq.d/                        505   e-142
SFL211 (SFL211Q) /CSM/SF/SFL2-A/SFL211Q.Seq.d/                        505   e-142
SFK703 (SFK703Q) /CSM/SF/SFK7-A/SFK703Q.Seq.d/                        505   e-142
SFK605 (SFK605Q) /CSM/SF/SFK6-A/SFK605Q.Seq.d/                        505   e-142
SFJ818 (SFJ818Q) /CSM/SF/SFJ8-A/SFJ818Q.Seq.d/                        505   e-142
SFJ734 (SFJ734Q) /CSM/SF/SFJ7-B/SFJ734Q.Seq.d/                        505   e-142
own update 2001. 9. 2
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.    42   6e-07  10
AC115598|AC115598.2 Dictyostelium discoideum chromosome 2 map 581427-735498 strain AX4, complete sequence.    46   7e-06  7
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    44   2e-05  13
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    46   6e-05  8
AC116963|AC116963.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.    38   5e-04  12
AC111403|AC111403.4 Rattus norvegicus clone CH230-95P17, WORKING DRAFT SEQUENCE, 1 ordered piece.    46   0.004  5
AC117080|AC117080.2 Dictyostelium discoideum chromosome 2 map complement(821514-735598) strain AX4, complete sequence.    34   0.004  7
AC116979|AC116979.3 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.    42   0.005  8
AC123513|AC123513.1 Dictyostelium discoideum chromosome 2 map 2779865-2840915 strain AX4, *** SEQUENCING IN PROGRESS ***, in ordered pieces.    42   0.008  7
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    44   0.009  8
dna update 2004. 9.24
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54JB0) RecName: Full=Protein DDI1 homolog;                          148   1e-34
AK067974_1(AK067974|pid:none) Oryza sativa Japonica Group cDNA c...    53   7e-06
BT058466_1(BT058466|pid:none) Salmo salar clone Contig1073 UV ex...    44   0.003
BC055645_1(BC055645|pid:none) Danio rerio neural precursor cell ...    44   0.004
BC078600_1(BC078600|pid:none) Xenopus laevis hypothetical protei...    44   0.004
BC076245_1(BC076245|pid:none) Danio rerio neural precursor cell ...    44   0.004
EF103394_1(EF103394|pid:none) Haliotis discus discus ubiquitin-l...    43   0.007
AB024034_5(AB024034|pid:none) Arabidopsis thaliana genomic DNA, ...    43   0.007
AY085083_1(AY085083|pid:none) Arabidopsis thaliana clone 12734 m...    43   0.007
DQ683059_1(DQ683059|pid:none) Synthetic construct DDI1 (DDI1) ge...    43   0.007
protein update 2009. 4.23
PSORT 

 psg: 0.87  gvh: 0.33  alm: 0.39  top: 0.50  tms: 0.00  mit: 0.24  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 28.0 %: nuclear
	 12.0 %: cytoskeletal
	  8.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for AFJ136 is cyt
5' end seq. ID AFJ136F
5' end seq. 
>AFJ136F.Seq
ACTGTTGGCCTACTGGTATCTATGCTATCCACACACCCAAAGTATTCATATTAATGGCAG
AAATAACAATATCAATTGAAAATGAAAATTTCATTCAAGTAAATTTACAACCAGATCAAA
CAGTTGAAGATCTAAAACGTAGAGTAGAATTTGAAACCACAATTTTAGTGAACAATCAAG
TTTTAACACTTGATGGAAAAGNNTTAGATAATGAAAAGAAATTATCAGACTATTCAATTA
AAGGTGGNGATTTCTTATTGATAACAAAAAATGTTTTAAGAGCACCACAACAAAGATCAC
AACAACCCCAACAACCACAACAACCACAACAACCACA----------
Length of 5' end seq. 337
3' end seq. ID AFJ136Z
3' end seq. 
>AFJ136Z.Seq
----------AACCACCTACCACTACCACTACCACTACTTCACCCCAAACTCCAACTAAT
GCCACTTCTCCTGTAAATAATAATAATAATAATAATAACAACAACAACAACACTAGACCT
CCAATTAATTCACCACCAGTTGCAAAGACACCGCAACCAACACCACAACCAACTACAAAT
CAATATGCTTCCGAATCCAATATCCAAACCCTTATTGGACTTGGTTGTTCAAGAGATAAG
GCAATTAGACTTTTACTCAAGCTAAAGGCAATG
Length of 3' end seq. 263
Connected seq. ID AFJ136P
Connected seq. 
>AFJ136P.Seq
ACTGTTGGCCTACTGGTATCTATGCTATCCACACACCCAAAGTATTCATATTAATGGCAG
AAATAACAATATCAATTGAAAATGAAAATTTCATTCAAGTAAATTTACAACCAGATCAAA
CAGTTGAAGATCTAAAACGTAGAGTAGAATTTGAAACCACAATTTTAGTGAACAATCAAG
TTTTAACACTTGATGGAAAAGNNTTAGATAATGAAAAGAAATTATCAGACTATTCAATTA
AAGGTGGNGATTTCTTATTGATAACAAAAAATGTTTTAAGAGCACCACAACAAAGATCAC
AACAACCCCAACAACCACAACAACCACAACAACCACA----------AACCACCTACCAC
TACCACTACCACTACTTCACCCCAAACTCCAACTAATGCCACTTCTCCTGTAAATAATAA
TAATAATAATAATAACAACAACAACAACACTAGACCTCCAATTAATTCACCACCAGTTGC
AAAGACACCGCAACCAACACCACAACCAACTACAAATCAATATGCTTCCGAATCCAATAT
CCAAACCCTTATTGGACTTGGTTGTTCAAGAGATAAGGCAATTAGACTTTTACTCAAGCT
AAAGGCAATG
Length of connected seq. 600
Full length Seq ID -
Full length Seq. -
Length of full length seq. -