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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFB128
Library AF
(Link to library)
Clone ID AFB128
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14931-1
Original site URL
Representative seq. ID AFB128F
(Link to Original site)
Representative DNA sequence 
>AFB128 (AFB128Q) /CSM/AF/AFB1-B/AFB128Q.Seq.d/
CACTGTTGGCCTACTGGTAATTTTTAACAAATCATTAATACAAAATGTCTTCAAATAAAA
TTGGTAATAAATTAAGTCTTGACAAGGTAGATGTAAAAGGTAAAAGAGTTTTAATTAGAG
TTGATTATAATGTACCATTAGATAAAAATAATAATATTACAAGTACTCAAAGAATTGATG
CATCAATTCCAACATTAGAATATTGTTTAAAGAATGGTGCTAAATCAATTGTATTGATGT
CACATTTAGGTCGTCCAGATGGTTTGGTTAAACCAGAGTATAGTTTGAAACCAGTGGTTA
AAGTATTGGAAGATCAATTAAAAAGACCAATTAAATTCTTATCAGATTGTGTTGGTGAAC
AAGTTGAAAAAGAATGTGCAAATCCAGAGGAAGGTACCGTTATTTTATTGGAGAATCTCC
GTTTCCACATTGAAGAGGAAGGTTCAGGTGTTGATGCAGAGGGTAAAAAGGTCAAAGCCA
ACCCAGAGAAAGTTAAAGAATTCCGTGAATCACTCACCAAGCTCGGTGATGTCTAXXXXX
XXXXX
sequence update 2001. 6. 1
Translated Amino Acid sequence 
llayw*fltnh*YKMSSNKIGNKLSLDKVDVKGKRVLIRVDYNVPLDKNNNITSTQRIDA
SIPTLEYCLKNGAKSIVLMSHLGRPDGLVKPEYSLKPVVKVLEDQLKRPIKFLSDCVGEQ
VEKECANPEEGTVILLENLRFHIEEEGSGVDAEGKKVKANPEKVKEFRESLTKLGDV---
Translated Amino Acid sequence (All Frames) 
Frame A:
hcwptgnf*qiintkclqiklvin*vltr*m*kvkef*leliimyh*ikiiilqvlkelm
hqfqh*niv*rmvlnqly*chi*vvqmvwlnqsiv*nqwlkywkin*kdqlnsyqivlvn
klkknvqiqrkvplfywrisvstlkrkvqvlmqrvkrskptqrklknsvnhspssvms--
-


Frame B:
tvgllvifnksliqnvfk*nw**iks*qgrckr*ksfn*s*l*ctir*k**yykyskn*c
insnirilfkewc*incidvtfrssrwfg*trv*fetsg*sigrsikktn*ilirlcw*t
s*krmcksrgryryfigespfph*rgrfrc*crg*kgqsqpres*rip*ithqar*c---


Frame C:
llayw*fltnh*YKMSSNKIGNKLSLDKVDVKGKRVLIRVDYNVPLDKNNNITSTQRIDA
SIPTLEYCLKNGAKSIVLMSHLGRPDGLVKPEYSLKPVVKVLEDQLKRPIKFLSDCVGEQ
VEKECANPEEGTVILLENLRFHIEEEGSGVDAEGKKVKANPEKVKEFRESLTKLGDV---
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFB128 (AFB128Q) /CSM/AF/AFB1-B/AFB128Q.Seq.d/                       1061   0.0
AFK457 (AFK457Q) /CSM/AF/AFK4-C/AFK457Q.Seq.d/                       1043   0.0
AFN807 (AFN807Q) /CSM/AF/AFN8-A/AFN807Q.Seq.d/                       1011   0.0
VFI829 (VFI829Q) /CSM/VF/VFI8-B/VFI829Q.Seq.d/                       1009   0.0
CFC236 (CFC236Q) /CSM/CF/CFC2-B/CFC236Q.Seq.d/                       1009   0.0
AFO316 (AFO316Q) /CSM/AF/AFO3-A/AFO316Q.Seq.d/                       1009   0.0
AFG647 (AFG647Q) /CSM/AF/AFG6-B/AFG647Q.Seq.d/                       1009   0.0
VFH746 (VFH746Q) /CSM/VF/VFH7-B/VFH746Q.Seq.d/                       1007   0.0
SFJ106 (SFJ106Q) /CSM/SF/SFJ1-A/SFJ106Q.Seq.d/                       1003   0.0
AFN348 (AFN348Q) /CSM/AF/AFN3-B/AFN348Q.Seq.d/                       1003   0.0
own update 2009. 4. 3
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AF316577|AF316577.2 Dictyostelium discoideum phosphoglycerate kinase (pgkA) mRNA, complete cds.   981  0.0  2
AF018637|AF018637.1 Dictyostelium discoideum phosphoglycerate kinase (pgkA) mRNA, partial cds.    76  5e-10  1
D12474|D12474.1 Candida maltosa C-PGK1 gene for phosphoglycerate kinase, complete cds.    40  0.002  2
AW713943|AW713943.1 h4e11ne.f1 Neurospora crassa evening cDNA library Neurospora crassa cDNA clone h4e11ne 5', mRNA sequence.    40  0.002  2
AW712348|AW712348.1 g1b07ne.f1 Neurospora crassa evening cDNA library Neurospora crassa cDNA clone g1b07ne 5', mRNA sequence.    40  0.002  2
AW712517|AW712517.1 f7b12ne.f2 Neurospora crassa evening cDNA library Neurospora crassa cDNA clone f7b12ne 5', mRNA sequence.    40  0.003  2
AW712302|AW712302.1 f9g10ne.f1 Neurospora crassa evening cDNA library Neurospora crassa cDNA clone f9g10ne 5', mRNA sequence.    40  0.003  2
AW711776|AW711776.1 f5g08ne.f1 Neurospora crassa evening cDNA library Neurospora crassa cDNA clone f5g08ne 5', mRNA sequence.    40  0.003  2
AW712696|AW712696.1 g2g12ne.f1 Neurospora crassa evening cDNA library Neurospora crassa cDNA clone g2g12ne 5', mRNA sequence.    40  0.003  2
AW713083|AW713083.1 g5d05ne.f1 Neurospora crassa evening cDNA library Neurospora crassa cDNA clone g5d05ne 5', mRNA sequence.    40  0.003  2
dna update 2003. 7.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q9GPM4) RecName: Full=Phosphoglycerate kinase;          EC=2.7....   323   1e-87
AB172722_1(AB172722|pid:none) Macaca fascicularis brain cDNA clo...   209   3e-53
(A5A6K4) RecName: Full=Phosphoglycerate kinase 1;          EC=2....   209   4e-53
CR926123_1(CR926123|pid:none) Pongo abelii mRNA; cDNA DKFZp459A2...   209   4e-53
(Q5NVB5) RecName: Full=Phosphoglycerate kinase 1;          EC=2....   209   4e-53
AY888092_1(AY888092|pid:none) Synthetic construct Homo sapiens c...   209   4e-53
CR456716_1(CR456716|pid:none) Homo sapiens full open reading fra...   209   4e-53
(P09411) RecName: Full=Phosphoglycerate kinase 1;          EC=2....   208   6e-53
(P50310) RecName: Full=Phosphoglycerate kinase 1;          EC=2....   208   8e-53
FJ236704_1(FJ236704|pid:none) Ovis aries phosphoglycerate kinase...   207   2e-52
protein update 2009. 3.26
PSORT 

 psg: 0.59  gvh: 0.22  alm: 0.52  top: 0.53  tms: 0.00  mit: 0.25  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 36.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial

>> prediction for AFB128 is nuc
5' end seq. ID AFB128F
5' end seq. 
>AFB128F.Seq
CACTGTTGGCCTACTGGTAATTTTTAACAAATCATTAATACAAAATGTCTTCAAATAAAA
TTGGTAATAAATTAAGTCTTGACAAGGTAGATGTAAAAGGTAAAAGAGTTTTAATTAGAG
TTGATTATAATGTACCATTAGATAAAAATAATAATATTACAAGTACTCAAAGAATTGATG
CATCAATTCCAACATTAGAATATTGTTTAAAGAATGGTGCTAAATCAATTGTATTGATGT
CACATTTAGGTCGTCCAGATGGTTTGGTTAAACCAGAGTATAGTTTGAAACCAGTGGTTA
AAGTATTGGAAGATCAATTAAAAAGACCAATTAAATTCTTATCAGATTGTGTTGGTGAAC
AAGTTGAAAAAGAATGTGCAAATCCAGAGGAAGGTACCGTTATTTTATTGGAGAATCTCC
GTTTCCACATTGAAGAGGAAGGTTCAGGTGTTGATGCAGAGGGTAAAAAGGTCAAAGCCA
ACCCAGAGAAAGTTAAAGAATTCCGTGAATCACTCACCAAGCTCGGTGATGTCTA-----
-----
Length of 5' end seq. 535
3' end seq. ID -
3' end seq. -
Length of 3' end seq. -
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -