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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHD227
Library SH
(Link to library)
Clone ID SHD227
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16361-1
Original site URL
Representative seq. ID SHD227P
(Link to Original site)
Representative DNA sequence 
>SHD227 (SHD227Q) /CSM/SH/SHD2-B/SHD227Q.Seq.d/
AATAATAATTATAATTTAACTAATAGTTAAATTAAAAAGAATAATTCCATATATTTTTTT
TTAATTCATATCTTTTTTTTTAAAATTTTTTTTTTTTAAAAAAAAAAATNGAAATAATXX
XXXXXXXXCTTCTCCATTAATTATGAGTATTTGGACTGTACCAATCGTTGTAAAAGATCC
AATCATTTTCATTCTCGTCGGTATGATTCCTGTAATGGTGTTACCAAGAGTCATTCAATA
TATGATTCTTAGAGCCAAGAGACCTTATGAAGCCGGTAAGAGTGGTCCATCACTTTGGGT
AGAAGCTACTGATCTTTGGAGAGCTGAACAAACTTTCTTTGGTTTTGCTGGTACTTATAT
TTCATCATGGAGAGAAGGTTCCGCTTCAATCGTTAAACTTCTTAAAGCTAGAAAGATTAG
TCGTCATAAGTTAGCCATGTGGAATTGGAAGAGAGATTTCGTAAAGAAACCAGTAGTTTG
TGAAGTTTTCCGTCAAACTAAATTGGTCAATGAAAATGATAATGCTCAAGAATCAAGTGG
TAAACATAAGGCTGAACAATCTTTCCGTACTTCAAACAAAGAATCTGATACCATTAAAAA
TTCAAGACTTTTCCTTCCAAATATTATCCTCTTTGTTGTTAATATCTTGGCTATGATGAG
TGCTGTACTTAGATTTAATTGTTTCCAAAATGATATGTGGCTTTTGGTTGTCGTAGCTGG
TTTCTCTTTCTCTACCTTATGGCATTTATGGTCTTTCATTCCAATGGCTCTTCGTCAAAG
TGAAAAACAATGGCCATATGCTTCTTCATACCATGCTCACAATATTGTTTTATT
sequence update 2002.10.25
Translated Amino Acid sequence 
iiiii*livklkriipyiff*FISFFLKFFFFKKKNXNN---

---SPLIMSIWTVPIVVKDPIIFILVGMIPVMVLPRVIQYMILRAKRPYEAGKSGPSLWV
EATDLWRAEQTFFGFAGTYISSWREGSASIVKLLKARKISRHKLAMWNWKRDFVKKPVVC
EVFRQTKLVNENDNAQESSGKHKAEQSFRTSNKESDTIKNSRLFLPNIILFVVNILAMMS
AVLRFNCFQNDMWLLVVVAGFSFSTLWHLWSFIPMALRQSEKQWPYASSYHAHNIVL
Translated Amino Acid sequence (All Frames) 
Frame A:
nnnynltns*ikknnsiyfflihifffkifff*kkkxk*---

---llh*l*vfglyqsl*kiqsfsfssv*fl*wcyqesfni*fleprdlmkpvrvvhhfg
*kllifgelnklslvllvlifhhgekvplqslnflklerlvvis*pcgigreis*rnq*f
vkfsvklnwsmkmimlknqvvnirlnnlsvlqtknliplkiqdfsfqilssllliswl**
vlyldlivskmicgfwls*lvslslpygiyglsfqwlfvkvknnghmllhtmltilfy

Frame B:
iiiii*livklkriipyiff*FISFFLKFFFFKKKNXNN---

---fsinyeyldctnrckrsnhfhsrrydscngvtkshsiyds*sqetl*sr*ewsitlg
rsy*sles*tnflwfcwylyfimerrfrfnr*ts*s*kd*ss*vshveleerfrketssl
*sfpsn*igq*k**csrikw*t*g*tifpyfkqri*yh*kfktfpskyyplcc*ylgyde
cct*i*lfpk*yvafgcrswflflylmafmvfhsngsssk*ktmaicffipcsqycfi

Frame C:
**l*fn**ln*ke*fhifffnsylff*nffflkkkxei---

---SPLIMSIWTVPIVVKDPIIFILVGMIPVMVLPRVIQYMILRAKRPYEAGKSGPSLWV
EATDLWRAEQTFFGFAGTYISSWREGSASIVKLLKARKISRHKLAMWNWKRDFVKKPVVC
EVFRQTKLVNENDNAQESSGKHKAEQSFRTSNKESDTIKNSRLFLPNIILFVVNILAMMS
AVLRFNCFQNDMWLLVVVAGFSFSTLWHLWSFIPMALRQSEKQWPYASSYHAHNIVL
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHK418 (SHK418Q) /CSM/SH/SHK4-A/SHK418Q.Seq.d/                       1400   0.0
SHG576 (SHG576Q) /CSM/SH/SHG5-D/SHG576Q.Seq.d/                       1400   0.0
SHG486 (SHG486Q) /CSM/SH/SHG4-D/SHG486Q.Seq.d/                       1400   0.0
SHG409 (SHG409Q) /CSM/SH/SHG4-A/SHG409Q.Seq.d/                       1400   0.0
SHG402 (SHG402Q) /CSM/SH/SHG4-A/SHG402Q.Seq.d/                       1400   0.0
SHD789 (SHD789Q) /CSM/SH/SHD7-D/SHD789Q.Seq.d/                       1400   0.0
SHD746 (SHD746Q) /CSM/SH/SHD7-B/SHD746Q.Seq.d/                       1400   0.0
SHD227 (SHD227Q) /CSM/SH/SHD2-B/SHD227Q.Seq.d/                       1400   0.0
SHC648 (SHC648Q) /CSM/SH/SHC6-B/SHC648Q.Seq.d/                       1400   0.0
SHC269 (SHC269Q) /CSM/SH/SHC2-C/SHC269Q.Seq.d/                       1400   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF163835|AF163835.1 Dictyostelium discoideum cellulose synthase (dcsA) mRNA, complete cds.  1400   0.0    1
AP007626|AP007626.1 Lotus corniculatus var. japonicus genomic DNA, chromosome 4, clone:LjT06G24, TM0839.    42   0.29   2
AC007485|AC007485.10 Homo sapiens chromosome 17, clone RP11-257O5, complete sequence.    38   2.0    3
AC023399|AC023399.4 Homo sapiens chromosome 17, clone RP11-780O8, complete sequence.    38   2.2    3
AP004545|AP004545.1 Lotus japonicus genomic DNA, chromosome 1, clone:LjT26E16, TM0103.    38   2.8    4
BH458878|BH458878.1 BOHEC38TF BOHE Brassica oleracea genomic clone BOHEC38, DNA sequence.    32   3.1    2
CO214588|CO214588.1 WS00929.B21_A19 IS-B-N-A-10 Picea engelmannii x Picea sitchensis cDNA clone WS00929_A19 3', mRNA sequence.    44   4.8    1
AC152510|AC152510.3 Bos taurus clone CH240-16D7, WORKING DRAFT SEQUENCE, 24 unordered pieces.    44   4.8    1
AC125647|AC125647.5 Rattus norvegicus clone CH230-231J3, WORKING DRAFT SEQUENCE, 2 unordered pieces.    44   4.8    1
AC119192|AC119192.3 Mus musculus clone RP24-178H23, WORKING DRAFT SEQUENCE, 6 unordered pieces.    44   4.8    1
dna update 2006. 1.29
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q9U720) RecName: Full=Cellulose synthase catalytic subunit A [U...   476   e-133
CP001279_992(CP001279|pid:none) Nautilia profundicola AmH, compl...    40   0.089
CP001048_907(CP001048|pid:none) Yersinia pseudotuberculosis PB1/...    35   2.2
CP001025_1222(CP001025|pid:none) Burkholderia ambifaria MC40-6 c...    35   2.2
CP000431_1654(CP000431|pid:none) Rhodococcus jostii RHA1, comple...    35   3.7
protein update 2009. 4.16
PSORT 

 psg: 0.58  gvh: 0.48  alm: 0.23  top: 0.73  tms: 0.20  mit: 0.34  mip: 0.11
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 1.00  m_ : 0.00 

	 24.0 %: plasma membrane
	 20.0 %: endoplasmic reticulum
	 16.0 %: nuclear
	 16.0 %: vacuolar
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: cytoplasmic

>> prediction for SHD227 is pla
5' end seq. ID SHD227F
5' end seq. 
>SHD227F.Seq
AATAATAATTATAATTTAACTAATAGTTAAATTAAAAAGAATAATTCCATATATTTTTTT
TTAATTCATATCTTTTTTTTTAAAATTTTTTTTTTTTAAAAAAAAAAATNGAAATAATNN
NNNNNNNN
Length of 5' end seq. 128
3' end seq. ID SHD227Z
3' end seq. 
>SHD227Z.Seq
NNNNNNNNNNCTTCTCCATTAATTATGAGTATTTGGACTGTACCAATCGTTGTAAAAGAT
CCAATCATTTTCATTCTCGTCGGTATGATTCCTGTAATGGTGTTACCAAGAGTCATTCAA
TATATGATTCTTAGAGCCAAGAGACCTTATGAAGCCGGTAAGAGTGGTCCATCACTTTGG
GTAGAAGCTACTGATCTTTGGAGAGCTGAACAAACTTTCTTTGGTTTTGCTGGTACTTAT
ATTTCATCATGGAGAGAAGGTTCCGCTTCAATCGTTAAACTTCTTAAAGCTAGAAAGATT
AGTCGTCATAAGTTAGCCATGTGGAATTGGAAGAGAGATTTCGTAAAGAAACCAGTAGTT
TGTGAAGTTTTCCGTCAAACTAAATTGGTCAATGAAAATGATAATGCTCAAGAATCAAGT
GGTAAACATAAGGCTGAACAATCTTTCCGTACTTCAAACAAAGAATCTGATACCATTAAA
AATTCAAGACTTTTCCTTCCAAATATTATCCTCTTTGTTGTTAATATCTTGGCTATGATG
AGTGCTGTACTTAGATTTAATTGTTTCCAAAATGATATGTGGCTTTTGGTTGTCGTAGCT
GGTTTCTCTTTCTCTACCTTATGGCATTTATGGTCTTTCATTCCAATGGCTCTTCGTCAA
AGTGAAAAACAATGGCCATATGCTTCTTCATACCATGCTCACAATATTGTTTTATT
Length of 3' end seq. 716
Connected seq. ID SHD227P
Connected seq. 
>SHD227P.Seq
AATAATAATTATAATTTAACTAATAGTTAAATTAAAAAGAATAATTCCATATATTTTTTT
TTAATTCATATCTTTTTTTTTAAAATTTTTTTTTTTTAAAAAAAAAAATNGAAATAAT--
--------CTTCTCCATTAATTATGAGTATTTGGACTGTACCAATCGTTGTAAAAGATCC
AATCATTTTCATTCTCGTCGGTATGATTCCTGTAATGGTGTTACCAAGAGTCATTCAATA
TATGATTCTTAGAGCCAAGAGACCTTATGAAGCCGGTAAGAGTGGTCCATCACTTTGGGT
AGAAGCTACTGATCTTTGGAGAGCTGAACAAACTTTCTTTGGTTTTGCTGGTACTTATAT
TTCATCATGGAGAGAAGGTTCCGCTTCAATCGTTAAACTTCTTAAAGCTAGAAAGATTAG
TCGTCATAAGTTAGCCATGTGGAATTGGAAGAGAGATTTCGTAAAGAAACCAGTAGTTTG
TGAAGTTTTCCGTCAAACTAAATTGGTCAATGAAAATGATAATGCTCAAGAATCAAGTGG
TAAACATAAGGCTGAACAATCTTTCCGTACTTCAAACAAAGAATCTGATACCATTAAAAA
TTCAAGACTTTTCCTTCCAAATATTATCCTCTTTGTTGTTAATATCTTGGCTATGATGAG
TGCTGTACTTAGATTTAATTGTTTCCAAAATGATATGTGGCTTTTGGTTGTCGTAGCTGG
TTTCTCTTTCTCTACCTTATGGCATTTATGGTCTTTCATTCCAATGGCTCTTCGTCAAAG
TGAAAAACAATGGCCATATGCTTCTTCATACCATGCTCACAATATTGTTTTATT
Length of connected seq. 824
Full length Seq ID -
Full length Seq. -
Length of full length seq. -