>SHD218 (SHD218Q) /CSM/SH/SHD2-A/SHD218Q.Seq.d/
ATCCCAATATAATCCATTAATATTAAATTAATTTCATTTTATTGCATGTAATATAATAAT
AAATAAAATAAAATAAAATAAAATAAAATAAAATAAAAATTTAAATAAAAAATAAAAAGA
ATAAAGAAAAATAAAACTCACAACACACAAATATTAAAAAAAACTATACTTATAAAAAAA
AATAAAAAATAAAAAATAAAAAAATAACCCACACACTCGAACTAATAATAAACAATAAAC
AAACACACACACACATACATACACACACCAATAAACTAAATATATAGTAATAATAATAAT
AATAAATGGATCAATTAATCCCAGTTATTAATAAACTTCAAGATGTATTTAATACATTAG
GATCAGACCCATTGGATTTACCACAAATCGTTGTTGTTGGTAGTCAATCTTCAGGTAAAA
GTAGTGTACTTGAAAATATAGTAGGTCGTGATTTTTTACCAAGAGGTTCTGGTATTGTAA
CAAGAAGACCTTTGATTTTACAATTAACTCATTTACCAATTGCTGATGATGGATCACAAA
CTCAAGAATGGGGTGAATTTTTACATAAACCAAATGATATGTTTTATGATTTCTCAGAGA
TTXXXXXXXXXXGTCCACAAAATATGCCTCCAAATCAATNTAAACCAAGTTCAATCCCAC
AAAATGGTCCAACCAATAATAATAATAATAACAATAATAATAATAGACAAGATCATCAAC
AAGGTTCTTTCTTTTCATCTTTTTTCAGAGCTTCACCTGATCCATCTTTAGGTCAATATG
GTGGTGCTAATAATAGTAATAATTCAAATAATCCAACATCACCAATTAATTCAAGTAGTA
ATAGCGGTAATAATTATAATACTTTTGGTGGTCAACAATCATCATCTTCATCATCACAAC
AATTACAACAATCATCACAATCACAATATAAAACTAGTTATAATAATAATAATAATAGTA
GCAGCAATAATAGTAGTTATAATCGTTATCAAGATGATTTCTATGGTAGAGGTGATAAAT
TAAATCAAGTACCATCAATTATTAAAGCACCAGATGATTTAACAAGTAAAGAGAAATTTG
AAACTGAATTAATTCGTGAACTTTTAATTTCCTATTTTAATATCGTTAAAAAGAATGTTA
AGGATTCAGTTCCAAAATCAATTATGCATTTCTTGGTCAATCAATCAAAAGAACACATTC
AAAATGAATTGGTTGCAGCCCTCTACAAAGAGGAATTGTTTGATGAATTATTAGAGGAAT
CACCACAAATCTCTAGTAAGAGAAAATCTTGTAAAGCTATGATCGAAATTTTAAGAAAAG
CAAAGAAATTATTAANAAATTAAAGATTTTAGAAATTAAAATATATTTAAATAAAATTTT
A

pniih*y*infillhvi***ik*nkik*nkiki*iknkknkek*nsqhtnikknytykkk
*kiknkkithtleliinnkqththihthtnklni*******MDQLIPVINKLQDVFNTLG
SDPLDLPQIVVVGSQSSGKSSVLENIVGRDFLPRGSGIVTRRPLILQLTHLPIADDGSQT
QEWGEFLHKPNDMFYDFSEI---

---PQNMPPNQXKPSSIPQNGPTNNNNNNNNNNRQDHQQGSFFSSFFRASPDPSLGQYGG
ANNSNNSNNPTSPINSSSNSGNNYNTFGGQQSSSSSSQQLQQSSQSQYKTSYNNNNNSSS
NNSSYNRYQDDFYGRGDKLNQVPSIIKAPDDLTSKEKFETELIRELLISYFNIVKKNVKD
SVPKSIMHFLVNQSKEHIQNELVAALYKEELFDELLEESPQISSKRKSCKAMIEILRKAK
KLLXN*rf*klkyi*ikf

Frame A:
ipi*siniklisfycm*ynnk*nkik*nkik*kfk*kikrikknkthntqilkktilikk
nkk*kikk*pthsn***tinkhthtyihtpin*iysnnnnnkwin*sqllinfkmylih*
dqthwiyhkslllvvnlqvkvvylki**vvifyqevlvl*qedl*fyn*liyqllmmdhk
lkngvnfyinqmicfmisqr---

---vhkiclqinxnqvqshkmvqpiiiiitiiiidkiinkvlsfhlfselhlihl*vnmv
vliiviiqiiqhhqliqvviaviiiillvvnnhhlhhhnnynnhhnhniklviiiiiiva
aiivviivikmismvevin*ikyhqllkhqmi*qvkrnlkln*fvnf*fpilislkrmlr
iqfqnqlciswsinqkntfkmnwlqpstkrnclmny*rnhhkslvrenlvkl*skf*ekq
rny*xikdfrn*nifk*nf

Frame B:
sqynpliln*fhfiacniiinkik*nkik*nknlnkk*ke*rkikltthky*kklyl*kk
iknkk*knnphtrtnnkq*tnththtythq*tkyiviiiiingsinpsy**tsrci*yir
irpigfttnrccw*sifr*k*ct*kysrs*fftkrfwycnkktfdftinsftnc**witn
srmg*ift*tk*yvl*flrd---

---stkyasksx*tkfnptkwsnq*****q****trsstrfflfiffqsft*sifrsiww
c*****fk*snitn*fk***r**l*yfwwstiiifiittittiititi*n*l*******q
q***l*slsr*flw*r**iksstiny*str*fnk*rei*n*ins*tfnflf*yr*kec*g
fsskinyaflgqsikrthsk*igcsplqrgiv**iirgittnl**ekil*sydrnfkksk
eiixklkileikiylnkil

Frame C:
pniih*y*infillhvi***ik*nkik*nkiki*iknkknkek*nsqhtnikknytykkk
*kiknkkithtleliinnkqththihthtnklni*******MDQLIPVINKLQDVFNTLG
SDPLDLPQIVVVGSQSSGKSSVLENIVGRDFLPRGSGIVTRRPLILQLTHLPIADDGSQT
QEWGEFLHKPNDMFYDFSEI---

---PQNMPPNQXKPSSIPQNGPTNNNNNNNNNNRQDHQQGSFFSSFFRASPDPSLGQYGG
ANNSNNSNNPTSPINSSSNSGNNYNTFGGQQSSSSSSQQLQQSSQSQYKTSYNNNNNSSS
NNSSYNRYQDDFYGRGDKLNQVPSIIKAPDDLTSKEKFETELIRELLISYFNIVKKNVKD
SVPKSIMHFLVNQSKEHIQNELVAALYKEELFDELLEESPQISSKRKSCKAMIEILRKAK
KLLXN*rf*klkyi*ikf

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSJ846 (VSJ846Q) /CSM/VS/VSJ8-B/VSJ846Q.Seq.d/                        686   0.0
VHN494 (VHN494Q) /CSM/VH/VHN4-D/VHN494Q.Seq.d/                        686   0.0
VHL684 (VHL684Q) /CSM/VH/VHL6-D/VHL684Q.Seq.d/                        686   0.0
VHL521 (VHL521Q) /CSM/VH/VHL5-A/VHL521Q.Seq.d/                        686   0.0
SSK388 (SSK388Q) /CSM/SS/SSK3-D/SSK388Q.Seq.d/                        686   0.0
SHF258 (SHF258Q) /CSM/SH/SHF2-C/SHF258Q.Seq.d/                        686   0.0
SHD218 (SHD218Q) /CSM/SH/SHD2-A/SHD218Q.Seq.d/                        686   0.0
SHC828 (SHC828Q) /CSM/SH/SHC8-B/SHC828Q.Seq.d/                        686   0.0
SHC669 (SHC669Q) /CSM/SH/SHC6-C/SHC669Q.Seq.d/                        686   0.0
SHC586 (SHC586Q) /CSM/SH/SHC5-D/SHC586Q.Seq.d/                        686   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X99669|X99669.1 Dictyostelium discoideum mRNA for dynamin homologue.   686   0.0    3
CZ287077|CZ287077.1 cp50e08.f Candida parapsilosis Random Genomic Library Candida parapsilosis genomic clone cp50e08, genomic survey sequence.    84   1e-20  3
CZ291729|CZ291729.1 cp77c12.r Candida parapsilosis Random Genomic Library Candida parapsilosis genomic clone cp77c12, genomic survey sequence.    84   1e-20  3
CJ370309|CJ370309.1 Molgula tectiformis cDNA, gastrula/neurula clone:mtga013n07, 5' end.    52   7e-14  4
CJ367705|CJ367705.1 Molgula tectiformis cDNA, gastrula/neurula clone:mtga004e02, 5' end.    52   1e-13  4
CJ347247|CJ347247.1 Molgula tectiformis cDNA, cleaving embryo clone:mtcl005f07, 5' end.    44   2e-11  4
CJ416026|CJ416026.1 Molgula tectiformis cDNA, larva clone:mtlv023f08, 5' end.    44   5e-11  4
AF153362|AF153362.1 Dictyostelium discoideum adenylyl cyclase (acrA) gene, complete cds.    36   8e-06  5
CO370716|CO370716.1 tah64b03.y1 Hydra EST UCI 5 Hydra magnipapillata cDNA 5' similar to SW:DYN2_MOUSE P39054 DYNAMIN 2 ;, mRNA sequence.    46   9e-06  2
AJ251163|AJ251163.1 Dictyostelium discoideum dymB gene for dynamin B homologue.    62   3e-05  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q94464) RecName: Full=Dynamin-A;  &X99669_1(X99669|pid:none)         272   2e-71
BC044291_1(BC044291|pid:none) Xenopus laevis similar to dynamin ...   116   2e-24
AF061795_1(AF061795|pid:none) Homo sapiens dynamin-like protein ...   111   7e-23
BC107763_1(BC107763|pid:none) Homo sapiens dynamin 1-like, mRNA ...   111   7e-23
AK293290_1(AK293290|pid:none) Homo sapiens cDNA FLJ59840 complet...   111   7e-23
AK298568_1(AK298568|pid:none) Homo sapiens cDNA FLJ59948 complet...   111   7e-23
BC024590_1(BC024590|pid:none) Homo sapiens dynamin 1-like, mRNA ...   111   7e-23
BC046374_1(BC046374|pid:none) Xenopus laevis dynamin 1-like, mRN...   111   7e-23
AB169569_1(AB169569|pid:none) Macaca fascicularis brain cDNA, cl...   111   7e-23
AF000430_1(AF000430|pid:none) Homo sapiens dynamin-like protein ...   111   7e-23

 psg: 0.70  gvh: 0.43  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.15  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 44.0 %: nuclear
	  4.0 %: mitochondrial

>> prediction for SHD218 is cyt

>SHD218F.Seq
ATCCCAATATAATCCATTAATATTAAATTAATTTCATTTTATTGCATGTAATATAATAAT
AAATAAAATAAAATAAAATAAAATAAAATAAAATAAAAATTTAAATAAAAAATAAAAAGA
ATAAAGAAAAATAAAACTCACAACACACAAATATTAAAAAAAACTATACTTATAAAAAAA
AATAAAAAATAAAAAATAAAAAAATAACCCACACACTCGAACTAATAATAAACAATAAAC
AAACACACACACACATACATACACACACCAATAAACTAAATATATAGTAATAATAATAAT
AATAAATGGATCAATTAATCCCAGTTATTAATAAACTTCAAGATGTATTTAATACATTAG
GATCAGACCCATTGGATTTACCACAAATCGTTGTTGTTGGTAGTCAATCTTCAGGTAAAA
GTAGTGTACTTGAAAATATAGTAGGTCGTGATTTTTTACCAAGAGGTTCTGGTATTGTAA
CAAGAAGACCTTTGATTTTACAATTAACTCATTTACCAATTGCTGATGATGGATCACAAA
CTCAAGAATGGGGTGAATTTTTACATAAACCAAATGATATGTTTTATGATTTCTCAGAGA
TTNNNNNNNNNN

>SHD218Z.Seq
NNNNNNNNNNGTCCACAAAATATGCCTCCAAATCAATNTAAACCAAGTTCAATCCCACAA
AATGGTCCAACCAATAATAATAATAATAACAATAATAATAATAGACAAGATCATCAACAA
GGTTCTTTCTTTTCATCTTTTTTCAGAGCTTCACCTGATCCATCTTTAGGTCAATATGGT
GGTGCTAATAATAGTAATAATTCAAATAATCCAACATCACCAATTAATTCAAGTAGTAAT
AGCGGTAATAATTATAATACTTTTGGTGGTCAACAATCATCATCTTCATCATCACAACAA
TTACAACAATCATCACAATCACAATATAAAACTAGTTATAATAATAATAATAATAGTAGC
AGCAATAATAGTAGTTATAATCGTTATCAAGATGATTTCTATGGTAGAGGTGATAAATTA
AATCAAGTACCATCAATTATTAAAGCACCAGATGATTTAACAAGTAAAGAGAAATTTGAA
ACTGAATTAATTCGTGAACTTTTAATTTCCTATTTTAATATCGTTAAAAAGAATGTTAAG
GATTCAGTTCCAAAATCAATTATGCATTTCTTGGTCAATCAATCAAAAGAACACATTCAA
AATGAATTGGTTGCAGCCCTCTACAAAGAGGAATTGTTTGATGAATTATTAGAGGAATCA
CCACAAATCTCTAGTAAGAGAAAATCTTGTAAAGCTATGATCGAAATTTTAAGAAAAGCA
AAGAAATTATTAANAAATTAAAGATTTTAGAAATTAAAATATATTTAAATAAAATTTTA

>SHD218P.Seq
ATCCCAATATAATCCATTAATATTAAATTAATTTCATTTTATTGCATGTAATATAATAAT
AAATAAAATAAAATAAAATAAAATAAAATAAAATAAAAATTTAAATAAAAAATAAAAAGA
ATAAAGAAAAATAAAACTCACAACACACAAATATTAAAAAAAACTATACTTATAAAAAAA
AATAAAAAATAAAAAATAAAAAAATAACCCACACACTCGAACTAATAATAAACAATAAAC
AAACACACACACACATACATACACACACCAATAAACTAAATATATAGTAATAATAATAAT
AATAAATGGATCAATTAATCCCAGTTATTAATAAACTTCAAGATGTATTTAATACATTAG
GATCAGACCCATTGGATTTACCACAAATCGTTGTTGTTGGTAGTCAATCTTCAGGTAAAA
GTAGTGTACTTGAAAATATAGTAGGTCGTGATTTTTTACCAAGAGGTTCTGGTATTGTAA
CAAGAAGACCTTTGATTTTACAATTAACTCATTTACCAATTGCTGATGATGGATCACAAA
CTCAAGAATGGGGTGAATTTTTACATAAACCAAATGATATGTTTTATGATTTCTCAGAGA
TT----------GTCCACAAAATATGCCTCCAAATCAATNTAAACCAAGTTCAATCCCAC
AAAATGGTCCAACCAATAATAATAATAATAACAATAATAATAATAGACAAGATCATCAAC
AAGGTTCTTTCTTTTCATCTTTTTTCAGAGCTTCACCTGATCCATCTTTAGGTCAATATG
GTGGTGCTAATAATAGTAATAATTCAAATAATCCAACATCACCAATTAATTCAAGTAGTA
ATAGCGGTAATAATTATAATACTTTTGGTGGTCAACAATCATCATCTTCATCATCACAAC
AATTACAACAATCATCACAATCACAATATAAAACTAGTTATAATAATAATAATAATAGTA
GCAGCAATAATAGTAGTTATAATCGTTATCAAGATGATTTCTATGGTAGAGGTGATAAAT
TAAATCAAGTACCATCAATTATTAAAGCACCAGATGATTTAACAAGTAAAGAGAAATTTG
AAACTGAATTAATTCGTGAACTTTTAATTTCCTATTTTAATATCGTTAAAAAGAATGTTA
AGGATTCAGTTCCAAAATCAATTATGCATTTCTTGGTCAATCAATCAAAAGAACACATTC
AAAATGAATTGGTTGCAGCCCTCTACAAAGAGGAATTGTTTGATGAATTATTAGAGGAAT
CACCACAAATCTCTAGTAAGAGAAAATCTTGTAAAGCTATGATCGAAATTTTAAGAAAAG
CAAAGAAATTATTAANAAATTAAAGATTTTAGAAATTAAAATATATTTAAATAAAATTTT
A