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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHD189
Library SH
(Link to library)
Clone ID SHD189
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16507-1
Original site URL
Representative seq. ID SHD189P
(Link to Original site)
Representative DNA sequence 
>SHD189 (SHD189Q) /CSM/SH/SHD1-D/SHD189Q.Seq.d/
AATGAAATTAATGGAAAAAAAACAAAAATAAATGATATTAAAAAATCAAAACCAAAACTA
TCATCAACATTATTAAATGTTCATATTGTTGCTCATACTCATGATGATGTTGGTTGGTTA
AAAACAGTTGATGAATATTATTATGGATCAAATATGTCAATTGCATTTGCAGGTGTTCAA
TATACTTTGGATACAGCCATTACCTGTTTATTGGCAAATCCAGAAAGAAAGTTTATTTAT
GTTGAAATTGCATTTTTCCAAAGATGGTGGGATGAACAAAGTACAACAATGCAAAATATT
GTTAAGGGTTTGGTTGAAAGTGGTCAATTGGAATTCATAAATGGTGGGTATTGTATGAAT
GATGAGGCTACTACCTACTATGACGATACTATTGACCAAATGACATTGGGCCATCAATTC
CTTTGGGAGAATTTCGGAGTTATGCCAAAGATAGGCTGGCACATAGATCCATTTGGCCAT
AGTGCAACACAAGCTCGTATCTTTGGTCAATTAGGATTCGATGCATTCATTATCGGTCGT
ATGGATTATCAAGATATCGAAGCTCGTTTAGAGAXXXXXXXXXXTCGTCCATCATGGAAT
TTANCCGTGGTTCAACCAACCAGTGGTAATTATGTACCTGTGAATGCTATTGCNTACATT
CAAGATCCTAATCAATCATTACAATTCACCATTGTCACTGATAGAAGTCGTGGATGTGCA
TCGTTAAGAGATGGTCAATTGGATATGATGATGCATCGTCGTACTTTGAAAGATGATGGT
CGTGGTGTTGGTCAACCAATGAATGAGTCAACTCAAATCGTTACAACTAGTAAATTGATT
TTCCATGATATTTCAAGTTATGCTCAATCTCATTATCGTCCTGCTGCACTCTCTTTATCA
CATCCATTACTCCCAATGTTTACAACCACTCAACAATCTTCAAACGATTGGAATTCTCAA
TATCAAGGTGTTTACTCACCATTGACTTCAGCCTCACCTTTACCAAATGGTTTAAAGATT
CAAACTCTACAATGGTTAGATAATCAAGATAATACCATACTTTTACGTATTGAAAATATT
TATCAAATTGATGGTCAAGATTCACAAGATCCACAAACTATCACTTTAGATCTTTCAACT
ATCTTTTCAACAATTACAATCACTTCTGCAACTGAAATGAATTTAACTGGTGTTCAAAAA
TTATCAAATTTATCAAGATTAAAATGGAAAACTGTTGATGGTAAAAACTATGATCATAAA
TCATCATCATCAACTAAAGAAGATTCTTCAAAGGTTTTGTTTTTACTTTTTCTCCAATGC
AAATCAGAACTTTTAAATTACTA
sequence update 2002.10.25
Translated Amino Acid sequence 
NEINGKKTKINDIKKSKPKLSSTLLNVHIVAHTHDDVGWLKTVDEYYYGSNMSIAFAGVQ
YTLDTAITCLLANPERKFIYVEIAFFQRWWDEQSTTMQNIVKGLVESGQLEFINGGYCMN
DEATTYYDDTIDQMTLGHQFLWENFGVMPKIGWHIDPFGHSATQARIFGQLGFDAFIIGR
MDYQDIEARLE---

---RPSWNLXVVQPTSGNYVPVNAIAYIQDPNQSLQFTIVTDRSRGCASLRDGQLDMMMH
RRTLKDDGRGVGQPMNESTQIVTTSKLIFHDISSYAQSHYRPAALSLSHPLLPMFTTTQQ
SSNDWNSQYQGVYSPLTSASPLPNGLKIQTLQWLDNQDNTILLRIENIYQIDGQDSQDPQ
TITLDLSTIFSTITITSATEMNLTGVQKLSNLSRLKWKTVDGKNYDHKSSSSTKEDSSKV
LFLLFLQCKSELLNY
Translated Amino Acid sequence (All Frames) 
Frame A:
NEINGKKTKINDIKKSKPKLSSTLLNVHIVAHTHDDVGWLKTVDEYYYGSNMSIAFAGVQ
YTLDTAITCLLANPERKFIYVEIAFFQRWWDEQSTTMQNIVKGLVESGQLEFINGGYCMN
DEATTYYDDTIDQMTLGHQFLWENFGVMPKIGWHIDPFGHSATQARIFGQLGFDAFIIGR
MDYQDIEARLE---

---ssimefxrgstnqw*lctcecycxhsrs*siitihhch**kswmcivkrwsigydda
ssyfer*wswcwstne*vnsnryn**idfp*yfklcsislsscctlfitsitpnvynhst
ifkrlefsisrclltidfsltftkwfkdsnstmvr*sr*yhtfty*kylsn*wsrftrst
nyhfrsfnylfnnynhfcn*nefnwcskiikfikikmenc*w*kl*s*iiiin*rrffkg
fvftfspmqirtfkll

Frame B:
mklmekkqk*milknqnqnyhqhy*mfilllilmmmlvg*kqlmniimdqicqlhlqvfn
ilwiqplpvywqiqkeslfmlklhfskdggmnkvqqckillrvwlkvvnwns*mvgiv*m
mrllptmtilltk*hwainsfgriselcqr*agt*ihlaivqhklvslvn*dsmhslsvv
wiikisklv*r---

---RPSWNLXVVQPTSGNYVPVNAIAYIQDPNQSLQFTIVTDRSRGCASLRDGQLDMMMH
RRTLKDDGRGVGQPMNESTQIVTTSKLIFHDISSYAQSHYRPAALSLSHPLLPMFTTTQQ
SSNDWNSQYQGVYSPLTSASPLPNGLKIQTLQWLDNQDNTILLRIENIYQIDGQDSQDPQ
TITLDLSTIFSTITITSATEMNLTGVQKLSNLSRLKWKTVDGKNYDHKSSSSTKEDSSKV
LFLLFLQCKSELLNY

Frame C:
*n*wkknknk*y*kiktktiiniikcsyccsys**cwlvkns**illwikyvncicrcsi
yfgyshylfigksrkkvylc*ncifpkmvg*tkynnakyc*gfg*kwsigihkwwvlye*
*gyyll*ryy*pndigpsiplgefrsyakdrlahrsiwp*cntssylwsirircihyrsy
glsryrssfr---

---vhhgixpwfnqpvvimyl*mllxtfkilinhynsplslievvdvhr*emvnwi**ci
vvl*kmmvvvlvnq*msqlkslqlvn*fsmifqvmlnliivllhslyhihysqclqplnn
lqtigilnikvfthh*lqphlyqmv*rfklyng*iikiipyfyvlkifiklmvkihkihk
lsl*ifqlsfqqlqsllqlk*i*lvfknyqiyqd*ngkllmvktmiinhhhqlkkilqrf
cfyffsnanqnf*it
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHD189 (SHD189Q) /CSM/SH/SHD1-D/SHD189Q.Seq.d/                       2478   0.0
VHQ167 (VHQ167Q) /CSM/VH/VHQ1-C/VHQ167Q.Seq.d/                       1493   0.0
VHK686 (VHK686Q) /CSM/VH/VHK6-D/VHK686Q.Seq.d/                       1493   0.0
VHH791 (VHH791Q) /CSM/VH/VHH7-D/VHH791Q.Seq.d/                       1493   0.0
VHE212 (VHE212Q) /CSM/VH/VHE2-A/VHE212Q.Seq.d/                       1493   0.0
VHB156 (VHB156Q) /CSM/VH/VHB1-C/VHB156Q.Seq.d/                       1493   0.0
AHE423 (AHE423Q) /CSM/AH/AHE4-A/AHE423Q.Seq.d/                       1493   0.0
VHE461 (VHE461Q) /CSM/VH/VHE4-C/VHE461Q.Seq.d/                       1487   0.0
AHJ372 (AHJ372Q) /CSM/AH/AHJ3-C/AHJ372Q.Seq.d/                       1485   0.0
AHE874 (AHE874Q) /CSM/AH/AHE8-D/AHE874Q.Seq.d/                       1479   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M82822|M82822.1 Dictyostelium discoideum alpha-mannosidase (manA) gene, complete cds.  1360   0.0    3
CK149800|CK149800.1 CsmgEST00336 Culicoides sonorensis female serum-fed midgut cDNA Library Culicoides sonorensis cDNA, mRNA sequence.   117   1e-23  3
DN814237|A Chain A, The Structure Of The Bovine Lysosomal A-Mannosidase Suggests A Novel Mechanism For Low Ph Activation, mRNA sequence.    52   1e-16  4
DN816506|A Chain A, The Structure Of The Bovine Lysosomal A-Mannosidase Suggests A Novel Mechanism For Low Ph Activation, mRNA sequence.    52   2e-16  4
CX633815|CX633815.1 taj44a03.y3 Hydra EST UCI 5 Hydra magnipapillata cDNA 5' similar to TR:Q93093 Q93093 TRUNCATED LYSOSOMAL ACID ALPHA-MANNOSIDASE ;, mRNA sequence.    52   2e-16  4
DN816232| truncated lysosomal acid alpha-mannosidase [Homo sapiens], mRNA sequence.    52   2e-16  4
DN811862| lysosomal alpha-mannosidase [Cavia porcellus], mRNA sequence.    52   3e-16  4
DT613126|A Chain A, The Structure Of The Bovine Lysosomal A-Mannosidase Suggests A Novel Mechanism For Low Ph Activation, mRNA sequence.    52   3e-16  4
DN815247| lysosomal alpha-mannosidase [Cavia porcellus], mRNA sequence.    52   3e-16  4
CB073433|CB073433.1 taa32h01.y1 Hydra EST -II Hydra magnipapillata cDNA 5' similar to TR:Q93093 Q93093 TRUNCATED LYSOSOMAL ACID ALPHA-MANNOSIDASE ;, mRNA sequence.    52   2e-15  4
dna update 2006. 1.28
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P34098) RecName: Full=Lysosomal alpha-mannosidase;          Sho...   386   e-109
M82822_1(M82822|pid:none) Dictyostelium discoideum alpha-mannosi...   385   e-105
(Q29451) RecName: Full=Lysosomal alpha-mannosidase;          Sho...   221   3e-56
AK291572_1(AK291572|pid:none) Homo sapiens cDNA FLJ76867 complet...   220   8e-56
U05572_1(U05572|pid:none) Human lysosomal alpha-mannosidase (MAN...   220   8e-56
AB209921_1(AB209921|pid:none) Homo sapiens mRNA for mannosidase,...   220   8e-56
AK068415_1(AK068415|pid:none) Oryza sativa Japonica Group cDNA c...   220   1e-55
AY099704_1(AY099704|pid:none) Arabidopsis thaliana alpha-mannosi...   220   1e-55
AB005230_20(AB005230|pid:none) Arabidopsis thaliana genomic DNA,...   220   1e-55
JC2200(JC2200)alpha-mannosidase (EC 3.2.1.24) precursor - human       219   2e-55
protein update 2009. 4.16
PSORT 

 psg: 0.41  gvh: 0.43  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 36.0 %: cytoplasmic
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for SHD189 is nuc
5' end seq. ID SHD189F
5' end seq. 
>SHD189F.Seq
AATGAAATTAATGGAAAAAAAACAAAAATAAATGATATTAAAAAATCAAAACCAAAACTA
TCATCAACATTATTAAATGTTCATATTGTTGCTCATACTCATGATGATGTTGGTTGGTTA
AAAACAGTTGATGAATATTATTATGGATCAAATATGTCAATTGCATTTGCAGGTGTTCAA
TATACTTTGGATACAGCCATTACCTGTTTATTGGCAAATCCAGAAAGAAAGTTTATTTAT
GTTGAAATTGCATTTTTCCAAAGATGGTGGGATGAACAAAGTACAACAATGCAAAATATT
GTTAAGGGTTTGGTTGAAAGTGGTCAATTGGAATTCATAAATGGTGGGTATTGTATGAAT
GATGAGGCTACTACCTACTATGACGATACTATTGACCAAATGACATTGGGCCATCAATTC
CTTTGGGAGAATTTCGGAGTTATGCCAAAGATAGGCTGGCACATAGATCCATTTGGCCAT
AGTGCAACACAAGCTCGTATCTTTGGTCAATTAGGATTCGATGCATTCATTATCGGTCGT
ATGGATTATCAAGATATCGAAGCTCGTTTAGAGANNNNNNNNNN
Length of 5' end seq. 584
3' end seq. ID SHD189Z
3' end seq. 
>SHD189Z.Seq
NNNNNNNNNNTCGTCCATCATGGAATTTANCCGTGGTTCAACCAACCAGTGGTAATTATG
TACCTGTGAATGCTATTGCNTACATTCAAGATCCTAATCAATCATTACAATTCACCATTG
TCACTGATAGAAGTCGTGGATGTGCATCGTTAAGAGATGGTCAATTGGATATGATGATGC
ATCGTCGTACTTTGAAAGATGATGGTCGTGGTGTTGGTCAACCAATGAATGAGTCAACTC
AAATCGTTACAACTAGTAAATTGATTTTCCATGATATTTCAAGTTATGCTCAATCTCATT
ATCGTCCTGCTGCACTCTCTTTATCACATCCATTACTCCCAATGTTTACAACCACTCAAC
AATCTTCAAACGATTGGAATTCTCAATATCAAGGTGTTTACTCACCATTGACTTCAGCCT
CACCTTTACCAAATGGTTTAAAGATTCAAACTCTACAATGGTTAGATAATCAAGATAATA
CCATACTTTTACGTATTGAAAATATTTATCAAATTGATGGTCAAGATTCACAAGATCCAC
AAACTATCACTTTAGATCTTTCAACTATCTTTTCAACAATTACAATCACTTCTGCAACTG
AAATGAATTTAACTGGTGTTCAAAAATTATCAAATTTATCAAGATTAAAATGGAAAACTG
TTGATGGTAAAAACTATGATCATAAATCATCATCATCAACTAAAGAAGATTCTTCAAAGG
TTTTGTTTTTACTTTTTCTCCAATGCAAATCAGAACTTTTAAATTACTA
Length of 3' end seq. 769
Connected seq. ID SHD189P
Connected seq. 
>SHD189P.Seq
AATGAAATTAATGGAAAAAAAACAAAAATAAATGATATTAAAAAATCAAAACCAAAACTA
TCATCAACATTATTAAATGTTCATATTGTTGCTCATACTCATGATGATGTTGGTTGGTTA
AAAACAGTTGATGAATATTATTATGGATCAAATATGTCAATTGCATTTGCAGGTGTTCAA
TATACTTTGGATACAGCCATTACCTGTTTATTGGCAAATCCAGAAAGAAAGTTTATTTAT
GTTGAAATTGCATTTTTCCAAAGATGGTGGGATGAACAAAGTACAACAATGCAAAATATT
GTTAAGGGTTTGGTTGAAAGTGGTCAATTGGAATTCATAAATGGTGGGTATTGTATGAAT
GATGAGGCTACTACCTACTATGACGATACTATTGACCAAATGACATTGGGCCATCAATTC
CTTTGGGAGAATTTCGGAGTTATGCCAAAGATAGGCTGGCACATAGATCCATTTGGCCAT
AGTGCAACACAAGCTCGTATCTTTGGTCAATTAGGATTCGATGCATTCATTATCGGTCGT
ATGGATTATCAAGATATCGAAGCTCGTTTAGAGA----------TCGTCCATCATGGAAT
TTANCCGTGGTTCAACCAACCAGTGGTAATTATGTACCTGTGAATGCTATTGCNTACATT
CAAGATCCTAATCAATCATTACAATTCACCATTGTCACTGATAGAAGTCGTGGATGTGCA
TCGTTAAGAGATGGTCAATTGGATATGATGATGCATCGTCGTACTTTGAAAGATGATGGT
CGTGGTGTTGGTCAACCAATGAATGAGTCAACTCAAATCGTTACAACTAGTAAATTGATT
TTCCATGATATTTCAAGTTATGCTCAATCTCATTATCGTCCTGCTGCACTCTCTTTATCA
CATCCATTACTCCCAATGTTTACAACCACTCAACAATCTTCAAACGATTGGAATTCTCAA
TATCAAGGTGTTTACTCACCATTGACTTCAGCCTCACCTTTACCAAATGGTTTAAAGATT
CAAACTCTACAATGGTTAGATAATCAAGATAATACCATACTTTTACGTATTGAAAATATT
TATCAAATTGATGGTCAAGATTCACAAGATCCACAAACTATCACTTTAGATCTTTCAACT
ATCTTTTCAACAATTACAATCACTTCTGCAACTGAAATGAATTTAACTGGTGTTCAAAAA
TTATCAAATTTATCAAGATTAAAATGGAAAACTGTTGATGGTAAAAACTATGATCATAAA
TCATCATCATCAACTAAAGAAGATTCTTCAAAGGTTTTGTTTTTACTTTTTCTCCAATGC
AAATCAGAACTTTTAAATTACTA
Length of connected seq. 1333
Full length Seq ID -
Full length Seq. -
Length of full length seq. -