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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHD186
Library SH
(Link to library)
Clone ID SHD186
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13024-1|Contig-U16344-1
Original site URL
Representative seq. ID SHD186P
(Link to Original site)
Representative DNA sequence 
>SHD186 (SHD186Q) /CSM/SH/SHD1-D/SHD186Q.Seq.d/
ATTCAATCAAACAAACAAAAAACAACAATTTAAAAAAACAATAATTTATAGTAAAGTAAA
AACAATTAAAGAATCACTAATTGTTTTTTTCTCCTCCTCACTATAAATTCCAATATTTTT
TTTTAAATTAATTTCCAGCTTTTCTGTTTTTTTACACAATCACAACCAACCCTATATCAT
TACTACCAACCCTATACCATTACAATCTTATTTATTAAATAATTATTATTTGTTGTATTA
TTTTAGTGTATATTTGTTGATTTAATATTTATTAGCAAGTAACAAACAAAAAAAAAAXXX
XXXXXXXTTGAATCAAAAGTTATTGATTTCACTCATGGTACCATTCAAGGTGTTGGTGCT
CTTAAATGTGGTACTTGTAACTTTGCCAATAAGATCGCTGCTAACCTCAACATTGTTGGT
AACGTTAAAATGGCCGCAACTGCAACTGTCTATGCAAACATTGATGCTTTAATGTCAATC
ACCACCTCAACCCAAACCTCAATCTCTCAATCATCAACCAAGACTGTCAACGGTAAGACC
ACCACTCAATCATCATCAAGCTCATCATCATCCATCTCTAAACAATTCGTTCCAATGATC
GTTTGTTCAGGTACTGCTGATGTTGCTGGTAACCTCAATGTCGTTGCCTCAGAAGCCAAT
CAAGCTAAAATTGCCTCTGGTACTGTATTCAAATTCATCTCAGCCCCAGTTGTTAAAGGT
AATGTCTCCTCAGTTAGCTGTCTCACCTCAAGCACTGCTGCTGCTGCTCAACAAGCTGCC
GGTGCTGCTGCTGCTGCTGCCCAAAAAGCTGCTATGGCTGCTGCCTCTAAAGTTTGGTCA
ATTGTAAATTCACCATGTAACTGTGAATGTGGATTGAAGAGATTATAAATTGATTCAACT
CCTCCTCTCCAATGACAATAAATTAAAATAATACAACTCATTCTTATTTTTCTTTATTCT
AACCAATGTATTTGTCAATTAACTTATTAACTTTTTAAACACACCCTTTTCAATTTTAAA
ACAATAAAAATTTACATATTAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
fnqtnkkqqfkktiiyskvktikeslivffsssl*ipifffklissfsvflhnhnqpyii
ttnpiplqsyllnnyyllyyfsvylli*YLLASNKQKK---

---ESKVIDFTHGTIQGVGALKCGTCNFANKIAANLNIVGNVKMAATATVYANIDALMSI
TTSTQTSISQSSTKTVNGKTTTQSSSSSSSSISKQFVPMIVCSGTADVAGNLNVVASEAN
QAKIASGTVFKFISAPVVKGNVSSVSCLTSSTAAAAQQAAGAAAAAAQKAAMAAASKVWS
IVNSPCNCECGLKRL*idstpplq*q*ikiiqliliflysnqcicqlty*lfkhtlfnfk
tikiyilk
Translated Amino Acid sequence (All Frames) 
Frame A:
iqsnkqktti*knnnl**sknn*ritncffllltinsniff*infqlfcfftqsqptlyh
yyqpytitilfik*llfvvlf*cifvdlifisk*qtkkk---

---lnqkllislmvpfkvlvllnvvlvtlpirslltstllvtlkwpqlqlsmqtlml*cq
sppqpkpqslnhqprlstvrpplnhhqahhhpslnnsfq*sfvqvllmllvtsmslpqkp
iklklplvlysnssqpqllkvmspqlavspqalllllnklpvlllllpkkllwllplkfg
ql*ihhvtvnvd*rdykliqlllsndnklk*ynsflfffiltnvfvn*linflntpfsil
kq*kfty*

Frame B:
fnqtnkkqqfkktiiyskvktikeslivffsssl*ipifffklissfsvflhnhnqpyii
ttnpiplqsyllnnyyllyyfsvylli*YLLASNKQKK---

---*iksy*fhswyhsrcwcs*mwyl*lcq*drc*pqhcw*r*ngrncnclckh*cfnvn
hhlnpnlnlsiinqdcqr*dhhsiiikliiihl*tirsndrlfryc*ccw*pqcrclrsq
ss*nclwyciqihlspsc*r*clls*lshlkhccccstscrccccccpkscygccl*slv
nckftm*l*mwieeiin*fnssspmtin*nntthsyfslf*pmylsinlltf*thpfqf*
nnknlhik

Frame C:
sikqtknnnlkkq*fivk*kqlknh*lffspphykfqyffln*fpaflffytittnpisl
lptlyhynliy*iiiicciilvyic*fniy*qvtnkkk---

---ESKVIDFTHGTIQGVGALKCGTCNFANKIAANLNIVGNVKMAATATVYANIDALMSI
TTSTQTSISQSSTKTVNGKTTTQSSSSSSSSISKQFVPMIVCSGTADVAGNLNVVASEAN
QAKIASGTVFKFISAPVVKGNVSSVSCLTSSTAAAAQQAAGAAAAAAQKAAMAAASKVWS
IVNSPCNCECGLKRL*idstpplq*q*ikiiqliliflysnqcicqlty*lfkhtlfnfk
tikiyilk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLE342 (SLE342Q) /CSM/SL/SLE3-B/SLE342Q.Seq.d/                        884   0.0
SLA101 (SLA101Q) /CSM/SL/SLA1-A/SLA101Q.Seq.d/                        884   0.0
SHL563 (SHL563Q) /CSM/SH/SHL5-C/SHL563Q.Seq.d/                        884   0.0
SHL307 (SHL307Q) /CSM/SH/SHL3-A/SHL307Q.Seq.d/                        884   0.0
SHL203 (SHL203Q) /CSM/SH/SHL2-A/SHL203Q.Seq.d/                        884   0.0
SHK865 (SHK865Q) /CSM/SH/SHK8-C/SHK865Q.Seq.d/                        884   0.0
SHK762 (SHK762Q) /CSM/SH/SHK7-C/SHK762Q.Seq.d/                        884   0.0
SHK650 (SHK650Q) /CSM/SH/SHK6-C/SHK650Q.Seq.d/                        884   0.0
SHK536 (SHK536Q) /CSM/SH/SHK5-B/SHK536Q.Seq.d/                        884   0.0
SHJ640 (SHJ640Q) /CSM/SH/SHJ6-B/SHJ640Q.Seq.d/                        884   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U83087|U83087.1 Dictyostelium discoideum rsc12 mRNA, partial cds.   884   0.0    2
AC022143|AC022143.7 Homo sapiens chromosome 19 clone CTD-2378H7, complete sequence.    48   0.045  2
AX344185|AX344185.1 Sequence 32 from Patent WO0200926.             40   0.076  4
AX251781|AX251781.1 Sequence 42 from Patent WO0168911.             40   0.076  4
BD452167|BD452167.1 Diagnosis of Diseases Associated with Cell Cycle.    40   0.076  4
BD460245|BD460245.1 Diagnosis of Diseases Associated with Cell Cycle.    40   0.076  4
AJ297856|AJ297856.1 Lucilia sericata partial bcd gene for bicoid protein.    50   0.098  1
AC008747|AC008747.5 Homo sapiens chromosome 19 clone CTD-2588C8, complete sequence.    48   0.30   3
AL845362|AL845362.4 Zebrafish DNA sequence from clone CH211-142H2 in linkage group 20.    38   0.30   3
AC170957|AC170957.2 Bos taurus clone CH240-254I20, *** SEQUENCING IN PROGRESS ***, 21 unordered pieces.    48   0.39   1
dna update 2006. 1.28
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U83087_1(U83087|pid:none) Dictyostelium discoideum rsc12 mRNA, p...   264   4e-69
AY530539_1(AY530539|pid:none) Mimulus ringens deficiens (DEF) mR...    34   9.1
protein update 2008.11.14
PSORT 

 psg: 0.65  gvh: 0.45  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.20  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 40.0 %: nuclear
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for SHD186 is cyt
5' end seq. ID SHD186F
5' end seq. 
>SHD186F.Seq
ATTCAATCAAACAAACAAAAAACAACAATTTAAAAAAACAATAATTTATAGTAAAGTAAA
AACAATTAAAGAATCACTAATTGTTTTTTTCTCCTCCTCACTATAAATTCCAATATTTTT
TTTTAAATTAATTTCCAGCTTTTCTGTTTTTTTACACAATCACAACCAACCCTATATCAT
TACTACCAACCCTATACCATTACAATCTTATTTATTAAATAATTATTATTTGTTGTATTA
TTTTAGTGTATATTTGTTGATTTAATATTTATTAGCAAGTAACAAACAAAAAAAAAANNN
NNNNNNN
Length of 5' end seq. 307
3' end seq. ID SHD186Z
3' end seq. 
>SHD186Z.Seq
NNNNNNNNNNTTGAATCAAAAGTTATTGATTTCACTCATGGTACCATTCAAGGTGTTGGT
GCTCTTAAATGTGGTACTTGTAACTTTGCCAATAAGATCGCTGCTAACCTCAACATTGTT
GGTAACGTTAAAATGGCCGCAACTGCAACTGTCTATGCAAACATTGATGCTTTAATGTCA
ATCACCACCTCAACCCAAACCTCAATCTCTCAATCATCAACCAAGACTGTCAACGGTAAG
ACCACCACTCAATCATCATCAAGCTCATCATCATCCATCTCTAAACAATTCGTTCCAATG
ATCGTTTGTTCAGGTACTGCTGATGTTGCTGGTAACCTCAATGTCGTTGCCTCAGAAGCC
AATCAAGCTAAAATTGCCTCTGGTACTGTATTCAAATTCATCTCAGCCCCAGTTGTTAAA
GGTAATGTCTCCTCAGTTAGCTGTCTCACCTCAAGCACTGCTGCTGCTGCTCAACAAGCT
GCCGGTGCTGCTGCTGCTGCTGCCCAAAAAGCTGCTATGGCTGCTGCCTCTAAAGTTTGG
TCAATTGTAAATTCACCATGTAACTGTGAATGTGGATTGAAGAGATTATAAATTGATTCA
ACTCCTCCTCTCCAATGACAATAAATTAAAATAATACAACTCATTCTTATTTTTCTTTAT
TCTAACCAATGTATTTGTCAATTAACTTATTAACTTTTTAAACACACCCTTTTCAATTTT
AAAACAATAAAAATTTACATATTAAAA
Length of 3' end seq. 747
Connected seq. ID SHD186P
Connected seq. 
>SHD186P.Seq
ATTCAATCAAACAAACAAAAAACAACAATTTAAAAAAACAATAATTTATAGTAAAGTAAA
AACAATTAAAGAATCACTAATTGTTTTTTTCTCCTCCTCACTATAAATTCCAATATTTTT
TTTTAAATTAATTTCCAGCTTTTCTGTTTTTTTACACAATCACAACCAACCCTATATCAT
TACTACCAACCCTATACCATTACAATCTTATTTATTAAATAATTATTATTTGTTGTATTA
TTTTAGTGTATATTTGTTGATTTAATATTTATTAGCAAGTAACAAACAAAAAAAAAA---
-------TTGAATCAAAAGTTATTGATTTCACTCATGGTACCATTCAAGGTGTTGGTGCT
CTTAAATGTGGTACTTGTAACTTTGCCAATAAGATCGCTGCTAACCTCAACATTGTTGGT
AACGTTAAAATGGCCGCAACTGCAACTGTCTATGCAAACATTGATGCTTTAATGTCAATC
ACCACCTCAACCCAAACCTCAATCTCTCAATCATCAACCAAGACTGTCAACGGTAAGACC
ACCACTCAATCATCATCAAGCTCATCATCATCCATCTCTAAACAATTCGTTCCAATGATC
GTTTGTTCAGGTACTGCTGATGTTGCTGGTAACCTCAATGTCGTTGCCTCAGAAGCCAAT
CAAGCTAAAATTGCCTCTGGTACTGTATTCAAATTCATCTCAGCCCCAGTTGTTAAAGGT
AATGTCTCCTCAGTTAGCTGTCTCACCTCAAGCACTGCTGCTGCTGCTCAACAAGCTGCC
GGTGCTGCTGCTGCTGCTGCCCAAAAAGCTGCTATGGCTGCTGCCTCTAAAGTTTGGTCA
ATTGTAAATTCACCATGTAACTGTGAATGTGGATTGAAGAGATTATAAATTGATTCAACT
CCTCCTCTCCAATGACAATAAATTAAAATAATACAACTCATTCTTATTTTTCTTTATTCT
AACCAATGTATTTGTCAATTAACTTATTAACTTTTTAAACACACCCTTTTCAATTTTAAA
ACAATAAAAATTTACATATTAAAA
Length of connected seq. 1034
Full length Seq ID -
Full length Seq. -
Length of full length seq. -