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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHD182
Library SH
(Link to library)
Clone ID SHD182
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12649-1|Contig-U16394-1
Original site URL
Representative seq. ID SHD182P
(Link to Original site)
Representative DNA sequence 
>SHD182 (SHD182Q) /CSM/SH/SHD1-D/SHD182Q.Seq.d/
AAAATAAAAAAAAAAAAAAAAANTGGNANTANTTAAAACCCACAATNGCAATAATAATTN
TAATAATAATAAATAATAATAATAATAATANCAATAAAATNGTANCCCCAATAAATAATA
ATNTTAATAATAATAATAATAATAATAATAATAATAATAAAATAAAAAATNTAATAATAA
TAATNGTAATAATNGTAATAATAATAATAAAATAATAATAATNGAAATAATTTCANTTTA
AAAAATAAATAAAATAAAAANGGGNAAAAATAAAAAAAAAANTAATTTTTTTTTTTAAAA
AATTAAAATTTTNAAAAAAAAAXXXXXXXXXXCAATGAAGATTGGGAATGCAAAGTTTCA
GANTTTGGTCTTAGTCGTTTTAATACCGCCGATAATTTAGAAACCCTCAGTAAGATTCGT
GGTACCTTTGCCTATTGTTCACCTGAAGTCNCCGTAGGTAATGGTAGTCTCTACACTACC
AAATTTGATATTTACAGTATTGGNATCGTNTTTTGGGAATTAGTAACTCGTGTTATCAAT
GGTGAATACTNTCGTCCCTACAGTGAATATTNTCNCATCNAAATGGNCTTTCNAATCATG
TTNAACTCAAAGGAAGGTTTAAGNCCAACCTTACCTCAAAATACTCCCCCTGGTNTTNAA
GCNC
sequence update 2002.10.25
Translated Amino Acid sequence 
nkkkkkxwxxlkptxaiiixiiinnnnnnxnkxvxpinnnxnnnnnnnnnnnkiknxiii
xvixviiiik***xk*fxfkk*ik*KXXKIKKKXIFFFKKLKFXKK---

---NEDWECKVSXFGLSRFNTADNLETLSKIRGTFAYCSPEVXVGNGSLYTTKFDIYSIG
IVFWELVTRVINGEYXRPYSEYXXIXMXFXIMXNSKEGLXPTLPQNTPPGXXA
Translated Amino Acid sequence (All Frames) 
Frame A:
kikkkkkxgxx*npqxq**x****iiiiiixikxxpq*iixliiiiiiiiiiik*kx***
*x**x*****nnnnxnnfxlknk*nkxgxk*kkx*ffflkn*nfxkk---

---q*rlgmqsfrxws*sf*yrr*frnpq*dswylcllft*sxrr*w*slhyqi*ylqyw
xrxlgisnscyqw*ilsslq*ixshxnglsnhvxlkgrfkxnltskyspwxxs

Frame B:
k*kkkkkxxxxkthnxnnnxnnnk*****xq*nxxpnk**x************nkkxnnn
nxnnxnnnnkiiixeiisx*kinkikxgknkkkxnfff*kikixkkk---

---NEDWECKVSXFGLSRFNTADNLETLSKIRGTFAYCSPEVXVGNGSLYTTKFDIYSIG
IVFWELVTRVINGEYXRPYSEYXXIXMXFXIMXNSKEGLXPTLPQNTPPGXXA

Frame C:
nkkkkkxwxxlkptxaiiixiiinnnnnnxnkxvxpinnnxnnnnnnnnnnnkiknxiii
xvixviiiik***xk*fxfkk*ik*KXXKIKKKXIFFFKKLKFXKK---

---mkignakfqxlvlvvlippii*kpsvrfvvplpivhlksp*vmvvstlpnliftvlx
sxfgn**lvlsmvntxvptvnixxskwxfxscxtqrkv*xqpylkilplvxkx
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHD182 (SHD182Q) /CSM/SH/SHD1-D/SHD182Q.Seq.d/                        571   e-162
SHE857 (SHE857Q) /CSM/SH/SHE8-C/SHE857Q.Seq.d/                        539   e-152
CHS225 (CHS225Q) /CSM/CH/CHS2-B/CHS225Q.Seq.d/                        539   e-152
CHP295 (CHP295Q) /CSM/CH/CHP2-D/CHP295Q.Seq.d/                        539   e-152
AHC678 (AHC678Q) /CSM/AH/AHC6-D/AHC678Q.Seq.d/                        539   e-152
SHF208 (SHF208Q) /CSM/SH/SHF2-A/SHF208Q.Seq.d/                        533   e-150
AHF423 (AHF423Q) /CSM/AH/AHF4-A/AHF423Q.Seq.d/                        533   e-150
SHK887 (SHK887Q) /CSM/SH/SHK8-D/SHK887Q.Seq.d/                        529   e-149
SHD120 (SHD120Q) /CSM/SH/SHD1-A/SHD120Q.Seq.d/                        525   e-148
AHJ120 (AHJ120Q) /CSM/AH/AHJ1-A/AHJ120Q.Seq.d/                        484   e-136
own update 2002.10.16
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AZ983291|AZ983291.1 2M0264H20F Mouse 10kb plasmid UUGC2M library Mus musculus genomicclone UUGC2M0264H20 F, genomic survey sequence.    44   3.8    1
AC122532|AC122532.4 Mus musculus chromosome 5 clone RP24-538A10, complete sequence.    44   3.8    1
dna update 2006. 1.28
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54XX5) RecName: Full=Probable serine/threonine-protein kinase ...   199   4e-50
(Q55GG4) RecName: Full=Probable serine/threonine-protein kinase ...   100   6e-20
(Q54RR9) RecName: Full=Probable serine/threonine-protein kinase ...    96   1e-18
(Q54G28) RecName: Full=Probable tyrosine-protein kinase DDB_G029...    95   2e-18
(Q54HC6) RecName: Full=Ankyrin repeat-containing protein kinase ...    92   1e-17
(Q86HG9) RecName: Full=Probable serine/threonine-protein kinase ...    73   6e-12
(Q54H46) RecName: Full=Probable serine/threonine-protein kinase ...    60   7e-08
(Q54R98) RecName: Full=Probable serine/threonine-protein kinase ...    59   9e-08
(Q3EDL4) RecName: Full=Putative serine/threonine-protein kinase ...    56   1e-06
A86146(A86146) hypothetical protein F22L4.8 - Arabidopsis thalia...    56   1e-06
protein update 2009. 4.16
PSORT 

 psg: 0.75  gvh: 0.37  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.28  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 28.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for SHD182 is cyt
5' end seq. ID SHD182F
5' end seq. 
>SHD182F.Seq
AAAATAAAAAAAAAAAAAAAAANTGGNANTANTTAAAACCCACAATNGCAATAATAATTN
TAATAATAATAAATAATAATAATAATAATANCAATAAAATNGTANCCCCAATAAATAATA
ATNTTAATAATAATAATAATAATAATAATAATAATAATAAAATAAAAAATNTAATAATAA
TAATNGTAATAATNGTAATAATAATAATAAAATAATAATAATNGAAATAATTTCANTTTA
AAAAATAAATAAAATAAAAANGGGNAAAAATAAAAAAAAAANTAATTTTTTTTTTTAAAA
AATTAAAATTTTNAAAAAAAAANNNNNNNNNN
Length of 5' end seq. 332
3' end seq. ID SHD182Z
3' end seq. 
>SHD182Z.Seq
NNNNNNNNNNCAATGAAGATTGGGAATGCAAAGTTTCAGANTTTGGTCTTAGTCGTTTTA
ATACCGCCGATAATTTAGAAACCCTCAGTAAGATTCGTGGTACCTTTGCCTATTGTTCAC
CTGAAGTCNCCGTAGGTAATGGTAGTCTCTACACTACCAAATTTGATATTTACAGTATTG
GNATCGTNTTTTGGGAATTAGTAACTCGTGTTATCAATGGTGAATACTNTCGTCCCTACA
GTGAATATTNTCNCATCNAAATGGNCTTTCNAATCATGTTNAACTCAAAGGAAGGTTTAA
GNCCAACCTTACCTCAAAATACTCCCCCTGGTNTTNAAGCNC
Length of 3' end seq. 342
Connected seq. ID SHD182P
Connected seq. 
>SHD182P.Seq
AAAATAAAAAAAAAAAAAAAAANTGGNANTANTTAAAACCCACAATNGCAATAATAATTN
TAATAATAATAAATAATAATAATAATAATANCAATAAAATNGTANCCCCAATAAATAATA
ATNTTAATAATAATAATAATAATAATAATAATAATAATAAAATAAAAAATNTAATAATAA
TAATNGTAATAATNGTAATAATAATAATAAAATAATAATAATNGAAATAATTTCANTTTA
AAAAATAAATAAAATAAAAANGGGNAAAAATAAAAAAAAAANTAATTTTTTTTTTTAAAA
AATTAAAATTTTNAAAAAAAAA----------CAATGAAGATTGGGAATGCAAAGTTTCA
GANTTTGGTCTTAGTCGTTTTAATACCGCCGATAATTTAGAAACCCTCAGTAAGATTCGT
GGTACCTTTGCCTATTGTTCACCTGAAGTCNCCGTAGGTAATGGTAGTCTCTACACTACC
AAATTTGATATTTACAGTATTGGNATCGTNTTTTGGGAATTAGTAACTCGTGTTATCAAT
GGTGAATACTNTCGTCCCTACAGTGAATATTNTCNCATCNAAATGGNCTTTCNAATCATG
TTNAACTCAAAGGAAGGTTTAAGNCCAACCTTACCTCAAAATACTCCCCCTGGTNTTNAA
GCNC
Length of connected seq. 654
Full length Seq ID -
Full length Seq. -
Length of full length seq. -