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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHD173
Library SH
(Link to library)
Clone ID SHD173
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16507-1
Original site URL
Representative seq. ID SHD173P
(Link to Original site)
Representative DNA sequence 
>SHD173 (SHD173Q) /CSM/SH/SHD1-D/SHD173Q.Seq.d/
CATAAAAANGGTAATAAAAAAATTATTTATATTAATTTTTTGTTNGTTTTTAATTATCAA
TGAAATTAATGGAAAAAAANCAAAAATAAATGATATTAAAAAATCAAAACCAAAACTATC
ATCANCATTATTAAANGTTCATATTGTTGCTCATACTCATGATGATGTTGGTTGGTTAAA
NACAGTTGATNAATATTATTATGGATCAAATATGTCAATTGCNTTTGCAGGNGTTCAATA
TACTTTGGATACAGCCATTACCTGTTTATTGGCAAATCCAAAAANAAAGTTTATTTATGT
TGAAATTGCATTTTTCCAAAGATGGNGGGATGAACAAAGTNCAACAATGCAAAATATTGT
TAAGGGTTTGGTTNAAAGNGGTCAATTGGAATTCATAAATGGXXXXXXXXXXATTATGTA
CCTGTGAATGCTATTGCCTACATTCAAGATCCTAATCAATCATTACAATTCACCATTGTC
ACTGATAGAAGTCGTGGATGTGCATCGTTAAGAGATGGTCAATTGGATATGATGATGCAT
CGTCGTACTTTGAAAGATGATGGTCGTGGTGTTGGTCAACCAATGAATGAGTCAACTCAA
ATCGTTACAACTAGTAAATTGATTTTCCATGATATTTCAAGTTATGCTCAATCTCATTAT
CGTCCTGCTGCACTCTCTTTATCACATCCATTACTCCCAATGTTTACAACCACTCAACAA
TCTTCAAACGATTGGAATTCTCAATATCAAGGTGTTTACTCACCATTGACTTCAGCCTCA
CCTTTACCAAATGGTTTAAAGATTCAAACTCTACAATGGTTAGATAATCAAGATAATACC
ATACTTTTACGTATTGAAAATATTTATCAAATTGATGGTCAAGATTCACAAGATCCACAA
ACTATCACTTTAGATCTTTCAACTATCTTTTCAACAATTACAATCACTTCTGCAACTGAA
ATGAATTTAACTGGTGTTCAAAAATTATCAAATTTATCAAGATTAAAATGGAAAACTGTT
GATGGTAAAAACTATGATCATAAATCATCATCATCAACNAAAAAAAAAAAAACAAAANNA
sequence update 2002.10.25
Translated Amino Acid sequence 
IKXVIKKLFILIFCXFLIINEINGKKXKINDIKKSKPKLSSXLLXVHIVAHTHDDVGWLX
TVDXYYYGSNMSIAFAGVQYTLDTAITCLLANPKXKFIYVEIAFFQRWXDEQSXTMQNIV
KGLVXXGQLEFIN---

---YVPVNAIAYIQDPNQSLQFTIVTDRSRGCASLRDGQLDMMMHRRTLKDDGRGVGQPM
NESTQIVTTSKLIFHDISSYAQSHYRPAALSLSHPLLPMFTTTQQSSNDWNSQYQGVYSP
LTSASPLPNGLKIQTLQWLDNQDNTILLRIENIYQIDGQDSQDPQTITLDLSTIFSTITI
TSATEMNLTGVQKLSNLSRLKWKTVDGKNYDHKSSSSTKKKKTKX
Translated Amino Acid sequence (All Frames) 
Frame A:
hkxgnkkiiyinflxvfnyq*n*wkkxknk*y*kiktktiixiikxsyccsys**cwlvk
xs*xillwikyvncxcrxsiyfgyshylfigkskxkvylc*ncifpkmxg*tkxnnakyc
*gfgxkxsigihkw---

---imyl*mllptfkilinhynsplslievvdvhr*emvnwi**civvl*kmmvvvlvnq
*msqlkslqlvn*fsmifqvmlnliivllhslyhihysqclqplnnlqtigilnikvfth
h*lqphlyqmv*rfklyng*iikiipyfyvlkifiklmvkihkihklsl*ifqlsfqqlq
sllqlk*i*lvfknyqiyqd*ngkllmvktmiinhhhqxkkkkqx

Frame B:
IKXVIKKLFILIFCXFLIINEINGKKXKINDIKKSKPKLSSXLLXVHIVAHTHDDVGWLX
TVDXYYYGSNMSIAFAGVQYTLDTAITCLLANPKXKFIYVEIAFFQRWXDEQSXTMQNIV
KGLVXXGQLEFIN---

---lctcecyclhsrs*siitihhch**kswmcivkrwsigyddassyfer*wswcwstn
e*vnsnryn**idfp*yfklcsislsscctlfitsitpnvynhstifkrlefsisrcllt
idfsltftkwfkdsnstmvr*sr*yhtfty*kylsn*wsrftrstnyhfrsfnylfnnyn
hfcn*nefnwcskiikfikikmenc*w*kl*s*iiiinxkkknkx

Frame C:
*kx**knyly*ffvxf*lsmklmekxqk*milknqnqnyhxhy*xfilllilmmmlvg*x
qlxniimdqicqlxlqxfnilwiqplpvywqiqkxslfmlklhfskdggmnkvqqckill
rvwxkxvnwns*m---

---YVPVNAIAYIQDPNQSLQFTIVTDRSRGCASLRDGQLDMMMHRRTLKDDGRGVGQPM
NESTQIVTTSKLIFHDISSYAQSHYRPAALSLSHPLLPMFTTTQQSSNDWNSQYQGVYSP
LTSASPLPNGLKIQTLQWLDNQDNTILLRIENIYQIDGQDSQDPQTITLDLSTIFSTITI
TSATEMNLTGVQKLSNLSRLKWKTVDGKNYDHKSSSSTKKKKTKX
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHQ167 (VHQ167Q) /CSM/VH/VHQ1-C/VHQ167Q.Seq.d/                       1281   0.0
VHP841 (VHP841Q) /CSM/VH/VHP8-B/VHP841Q.Seq.d/                       1281   0.0
VHP443 (VHP443Q) /CSM/VH/VHP4-B/VHP443Q.Seq.d/                       1281   0.0
VHP436 (VHP436Q) /CSM/VH/VHP4-B/VHP436Q.Seq.d/                       1281   0.0
VHO880 (VHO880Q) /CSM/VH/VHO8-D/VHO880Q.Seq.d/                       1281   0.0
VHO643 (VHO643Q) /CSM/VH/VHO6-B/VHO643Q.Seq.d/                       1281   0.0
VHN718 (VHN718Q) /CSM/VH/VHN7-A/VHN718Q.Seq.d/                       1281   0.0
VHN593 (VHN593Q) /CSM/VH/VHN5-D/VHN593Q.Seq.d/                       1281   0.0
VHM790 (VHM790Q) /CSM/VH/VHM7-D/VHM790Q.Seq.d/                       1281   0.0
VHK686 (VHK686Q) /CSM/VH/VHK6-D/VHK686Q.Seq.d/                       1281   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M82822|M82822.1 Dictyostelium discoideum alpha-mannosidase (manA) gene, complete cds.  1249   0.0    1
CK149800|CK149800.1 CsmgEST00336 Culicoides sonorensis female serum-fed midgut cDNA Library Culicoides sonorensis cDNA, mRNA sequence.   107   7e-20  2
CV682907|CV682907.1 service43_H06_sjs2-16-2B11-T3 SJS Schistosoma japonicum cDNA, mRNA sequence.    70   4e-11  2
CV688215|CV688215.1 sjs2-16-2_G01_sjs2-16-2G01-T3 SJS Schistosoma japonicum cDNA, mRNA sequence.    70   4e-11  2
CV682854|CV682854.1 service30_G09_sjs2-14-10C09-T3 SJS Schistosoma japonicum cDNA, mRNA sequence.    70   4e-11  2
BU798369|BU798369.1 SJF2ADB01 SJF Schistosoma japonicum cDNA, mRNA sequence.    70   5e-11  2
BU798139|BU798139.1 SJF2AAF09 SJF Schistosoma japonicum cDNA, mRNA sequence.    70   5e-11  2
BU804021|BU804021.1 SJFBFG06 SJF Schistosoma japonicum cDNA, mRNA sequence.    70   5e-11  2
BW354577|BW354577.1 Ciona intestinalis cDNA, clone:cima804e24, 5'end, single read.    62   2e-09  2
BW480516|BW480516.1 Ciona intestinalis cDNA, clone:cima037o04, 5'end, single read.    62   3e-09  2
dna update 2006. 1.28
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P34098) RecName: Full=Lysosomal alpha-mannosidase;          Sho...   353   5e-96
M82822_1(M82822|pid:none) Dictyostelium discoideum alpha-mannosi...   350   6e-95
(Q29451) RecName: Full=Lysosomal alpha-mannosidase;          Sho...   115   3e-24
AK291572_1(AK291572|pid:none) Homo sapiens cDNA FLJ76867 complet...   114   6e-24
U05572_1(U05572|pid:none) Human lysosomal alpha-mannosidase (MAN...   114   6e-24
AB209921_1(AB209921|pid:none) Homo sapiens mRNA for mannosidase,...   114   6e-24
AY036155_1(AY036155|pid:none) Cavia porcellus lysosomal alpha-ma...   113   1e-23
(Q8VHC8) RecName: Full=Lysosomal alpha-mannosidase;          Sho...   113   1e-23
JC2200(JC2200)alpha-mannosidase (EC 3.2.1.24) precursor - human       112   2e-23
AE014134_1973(AE014134|pid:none) Drosophila melanogaster chromos...   112   2e-23
protein update 2009. 4.16
PSORT 

 psg: 0.98  gvh: 0.56  alm: 0.23  top: 0.47  tms: 0.07  mit: 0.36  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 40.0 %: mitochondrial
	 20.0 %: nuclear
	 16.0 %: Golgi
	 12.0 %: cytoplasmic
	  8.0 %: endoplasmic reticulum
	  4.0 %: vacuolar

>> prediction for SHD173 is mit
5' end seq. ID SHD173F
5' end seq. 
>SHD173F.Seq
CATAAAAANGGTAATAAAAAAATTATTTATATTAATTTTTTGTTNGTTTTTAATTATCAA
TGAAATTAATGGAAAAAAANCAAAAATAAATGATATTAAAAAATCAAAACCAAAACTATC
ATCANCATTATTAAANGTTCATATTGTTGCTCATACTCATGATGATGTTGGTTGGTTAAA
NACAGTTGATNAATATTATTATGGATCAAATATGTCAATTGCNTTTGCAGGNGTTCAATA
TACTTTGGATACAGCCATTACCTGTTTATTGGCAAATCCAAAAANAAAGTTTATTTATGT
TGAAATTGCATTTTTCCAAAGATGGNGGGATGAACAAAGTNCAACAATGCAAAATATTGT
TAAGGGTTTGGTTNAAAGNGGTCAATTGGAATTCATAAATGGNNNNNNNNNN
Length of 5' end seq. 412
3' end seq. ID SHD173Z
3' end seq. 
>SHD173Z.Seq
NNNNNNNNNNATTATGTACCTGTGAATGCTATTGCCTACATTCAAGATCCTAATCAATCA
TTACAATTCACCATTGTCACTGATAGAAGTCGTGGATGTGCATCGTTAAGAGATGGTCAA
TTGGATATGATGATGCATCGTCGTACTTTGAAAGATGATGGTCGTGGTGTTGGTCAACCA
ATGAATGAGTCAACTCAAATCGTTACAACTAGTAAATTGATTTTCCATGATATTTCAAGT
TATGCTCAATCTCATTATCGTCCTGCTGCACTCTCTTTATCACATCCATTACTCCCAATG
TTTACAACCACTCAACAATCTTCAAACGATTGGAATTCTCAATATCAAGGTGTTTACTCA
CCATTGACTTCAGCCTCACCTTTACCAAATGGTTTAAAGATTCAAACTCTACAATGGTTA
GATAATCAAGATAATACCATACTTTTACGTATTGAAAATATTTATCAAATTGATGGTCAA
GATTCACAAGATCCACAAACTATCACTTTAGATCTTTCAACTATCTTTTCAACAATTACA
ATCACTTCTGCAACTGAAATGAATTTAACTGGTGTTCAAAAATTATCAAATTTATCAAGA
TTAAAATGGAAAACTGTTGATGGTAAAAACTATGATCATAAATCATCATCATCAACNAAA
AAAAAAAAAACAAAANNA
Length of 3' end seq. 678
Connected seq. ID SHD173P
Connected seq. 
>SHD173P.Seq
CATAAAAANGGTAATAAAAAAATTATTTATATTAATTTTTTGTTNGTTTTTAATTATCAA
TGAAATTAATGGAAAAAAANCAAAAATAAATGATATTAAAAAATCAAAACCAAAACTATC
ATCANCATTATTAAANGTTCATATTGTTGCTCATACTCATGATGATGTTGGTTGGTTAAA
NACAGTTGATNAATATTATTATGGATCAAATATGTCAATTGCNTTTGCAGGNGTTCAATA
TACTTTGGATACAGCCATTACCTGTTTATTGGCAAATCCAAAAANAAAGTTTATTTATGT
TGAAATTGCATTTTTCCAAAGATGGNGGGATGAACAAAGTNCAACAATGCAAAATATTGT
TAAGGGTTTGGTTNAAAGNGGTCAATTGGAATTCATAAATGG----------ATTATGTA
CCTGTGAATGCTATTGCCTACATTCAAGATCCTAATCAATCATTACAATTCACCATTGTC
ACTGATAGAAGTCGTGGATGTGCATCGTTAAGAGATGGTCAATTGGATATGATGATGCAT
CGTCGTACTTTGAAAGATGATGGTCGTGGTGTTGGTCAACCAATGAATGAGTCAACTCAA
ATCGTTACAACTAGTAAATTGATTTTCCATGATATTTCAAGTTATGCTCAATCTCATTAT
CGTCCTGCTGCACTCTCTTTATCACATCCATTACTCCCAATGTTTACAACCACTCAACAA
TCTTCAAACGATTGGAATTCTCAATATCAAGGTGTTTACTCACCATTGACTTCAGCCTCA
CCTTTACCAAATGGTTTAAAGATTCAAACTCTACAATGGTTAGATAATCAAGATAATACC
ATACTTTTACGTATTGAAAATATTTATCAAATTGATGGTCAAGATTCACAAGATCCACAA
ACTATCACTTTAGATCTTTCAACTATCTTTTCAACAATTACAATCACTTCTGCAACTGAA
ATGAATTTAACTGGTGTTCAAAAATTATCAAATTTATCAAGATTAAAATGGAAAACTGTT
GATGGTAAAAACTATGATCATAAATCATCATCATCAACNAAAAAAAAAAAAACAAAANNA
Length of connected seq. 1070
Full length Seq ID -
Full length Seq. -
Length of full length seq. -