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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHD168
Library SH
(Link to library)
Clone ID SHD168
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16308-1
Original site URL
Representative seq. ID SHD168P
(Link to Original site)
Representative DNA sequence 
>SHD168 (SHD168Q) /CSM/SH/SHD1-C/SHD168Q.Seq.d/
AAAATAAAATTCATTTAATAATAATAATTTAATTTTTTTCATAGTATTCAAAGATATAAT
GAAAAACTTTTATTATTTTATATTAATTTTATTATTTTTTAATGAAGTTTGCTATTCATT
AAAAGATGGTGAAATTAAACTTCATTTAATTCCACATTCACATTGTGATTCTGGATGGAC
ATCAACAATGAACGAATATTATATGGGTCAAGTTAAAAGTATTATATCATCAATGGTTCA
ATCATTAAATGTTGAATCAAATCCACCTAGAAAATTTGTTTGGTCTGAAATTGGATTTTT
AGAACAATGGTGGGATGATATGCCAATTGAAATTAAAAATGATTTTATAAAACATGTTAA
AAATGATAGAATTGAATTTGTAAATGGTGGATGGGTAATGAATGATGAAGCATGTGCAAG
TTTAGAAAGTGGTAATTAGACAATTATCAAATGGACATAAATTTATTAGAGAGAAATTTG
GAAAACAACCAGAGAGTGGATGGCAAATTGATCCATTTGGACATTCATCATTGACACCAA
CATTACAAGCTCAATTTGGTTATAAACATGTTGTATTGAATCGTATTCACTATGAATTGA
AAAAGAAATTTAAAGAAGAAXXXXXXXXXXGTTCNATTAAATTAAGAANTCATAATATTG
ATNNTAATAATCNATCTCCAATCNGTTTTGATTTANATGGCTNATTCTCTTTTATCAAAT
CAATTAAATCAATTAAAGAATANAATTTATNTNTAATCAATANATTCGTTGATAATAATT
ATTGATAATAGNATT
sequence update 2002.10.25
Translated Amino Acid sequence 
nkihliiii*ffs*yski**ktfiily*fyyflmkfaih*kmvklnfi*fhihivildgh
qq*tniiwvklkvlyhqwfnh*mlnqihlenlfglkldf*nnggmicqlklkmil*nmlk
mielnl*mvdg**mmkhvqv*KVVIRQLSNGHKFIREKFGKQPESGWQIDPFGHSSLTPT
LQAQFGYKHVVLNRIHYELKKKFKEE---

---SIKLRXHNIDXNNXSPIXFDLXGXFSFIKSIKSIKEXNLXXINXFVDNNY***x
Translated Amino Acid sequence (All Frames) 
Frame A:
kikfi****fnffhsiqryneklllfyinfiif**sllfikrw*n*tsfnstftl*fwmd
innerilygss*kyyiingsiikc*ikst*kiclv*nwifrtmvg*yan*n*k*fyktc*
k**n*ickwwmgne**smckfrkw*ldnyqmdinllernlennqrvdgklihldihh*hq
hyklnlvinmly*ivftmn*krnlkk---

---vxln*exiilxxiixlqsvlixmaxsllsnqlnqlknxiyx*sixsliiiidnxi

Frame B:
k*nsfnnnnliffivfkdimknfyyfilillffnevcyslkdgeiklhliphshcdsgwt
stmneyymgqvksiissmvqslnvesnpprkfvwseigfleqwwddmpieikndfikhvk
ndriefvnggwvmndeacaslesgn*tiikwt*iy*reiwkttrewman*siwtfiidtn
itssiwl*tcciesysl*iekei*rr---

---fx*ikxs*y*x**sisnxf*fxwlilfyqin*in*rixfxxnqxir***lliix

Frame C:
nkihliiii*ffs*yski**ktfiily*fyyflmkfaih*kmvklnfi*fhihivildgh
qq*tniiwvklkvlyhqwfnh*mlnqihlenlfglkldf*nnggmicqlklkmil*nmlk
mielnl*mvdg**mmkhvqv*KVVIRQLSNGHKFIREKFGKQPESGWQIDPFGHSSLTPT
LQAQFGYKHVVLNRIHYELKKKFKEE---

---SIKLRXHNIDXNNXSPIXFDLXGXFSFIKSIKSIKEXNLXXINXFVDNNY***x
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHD168 (SHD168Q) /CSM/SH/SHD1-C/SHD168Q.Seq.d/                       1239   0.0
SHL340 (SHL340Q) /CSM/SH/SHL3-B/SHL340Q.Seq.d/                       1013   0.0
SHL163 (SHL163Q) /CSM/SH/SHL1-C/SHL163Q.Seq.d/                       1013   0.0
SHK514 (SHK514Q) /CSM/SH/SHK5-A/SHK514Q.Seq.d/                       1013   0.0
SHK365 (SHK365Q) /CSM/SH/SHK3-C/SHK365Q.Seq.d/                       1013   0.0
SHJ890 (SHJ890Q) /CSM/SH/SHJ8-D/SHJ890Q.Seq.d/                       1013   0.0
SHJ720 (SHJ720Q) /CSM/SH/SHJ7-A/SHJ720Q.Seq.d/                       1013   0.0
SHJ609 (SHJ609Q) /CSM/SH/SHJ6-A/SHJ609Q.Seq.d/                       1013   0.0
SHJ369 (SHJ369Q) /CSM/SH/SHJ3-C/SHJ369Q.Seq.d/                       1013   0.0
SHI833 (SHI833Q) /CSM/SH/SHI8-B/SHI833Q.Seq.d/                       1013   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

CF445746|CF445746.1 EST682091 normalized cDNA library of onion Allium cepa cDNA cloneACAHS20, mRNA sequence.    60   2e-08  2
BQ593037|BQ593037.1 E012375-024-028-C05-SP6 MPIZ-ADIS-024-developing root Beta vulgaris cDNA clone 024-028-C05 5-PRIME, mRNA sequence.    44   4e-06  2
CF196667|CF196667.1 Ir64bi1.6_B04 IR64-BI Oryza sativa (indica cultivar-group) cDNA clone IR64-BI1.6B04 5', mRNA sequence.    56   5e-05  2
BQ141173|BQ141173.1 NF016G07PH1F1056 Phoma-infected Medicago truncatula cDNA clone NF016G07PH 5', mRNA sequence.    58   3e-04  1
BG449666|BG449666.1 NF055D11IN1F1092 Insect herbivory Medicago truncatula cDNA clone NF055D11IN 5', mRNA sequence.    58   3e-04  1
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    36   6e-04  7
DN812276|M2A1_RAT Alpha-mannosidase II (Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase) (MAN II) (Golgi alpha-mannosidase II) (Mannosidase alpha class 2A member 1), mRNA sequence.    56   0.001  1
CV688215|CV688215.1 sjs2-16-2_G01_sjs2-16-2G01-T3 SJS Schistosoma japonicum cDNA, mRNA sequence.    56   0.001  1
CV682907|CV682907.1 service43_H06_sjs2-16-2B11-T3 SJS Schistosoma japonicum cDNA, mRNA sequence.    56   0.001  1
CV682854|CV682854.1 service30_G09_sjs2-14-10C09-T3 SJS Schistosoma japonicum cDNA, mRNA sequence.    56   0.001  1
dna update 2006. 1.28
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54YC4) RecName: Full=Alpha-mannosidase D;          EC=3.2.1.24...    96   1e-28
(Q54YF7) RecName: Full=Alpha-mannosidase B;          EC=3.2.1.24...    87   3e-28
BC142656_1(BC142656|pid:none) Homo sapiens mannosidase, alpha, c...    91   2e-27
AB011474_23(AB011474|pid:none) Arabidopsis thaliana genomic DNA,...    95   2e-27
(P27046) RecName: Full=Alpha-mannosidase 2;          EC=3.2.1.11...    87   3e-27
AC148217_12(AC148217|pid:none) Medicago truncatula clone mth2-20...    92   6e-27
(P28494) RecName: Full=Alpha-mannosidase 2;          EC=3.2.1.11...    86   1e-26
M24353_1(M24353|pid:none) Rattus norvegicus alpha-mannosidase II...    86   1e-26
AL391146_7(AL391146|pid:none) Arabidopsis thaliana DNA chromosom...    95   2e-26
AF107018_1(AF107018|pid:none) Mus musculus alpha-mannosidase IIx...    89   2e-26
protein update 2009. 4.16
PSORT 

 psg: 0.62  gvh: 0.50  alm: 0.49  top: 0.53  tms: 0.00  mit: 0.43  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: mitochondrial
	 20.0 %: nuclear
	 16.0 %: cytoplasmic
	  4.0 %: plasma membrane

>> prediction for SHD168 is mit
5' end seq. ID SHD168F
5' end seq. 
>SHD168F.Seq
AAAATAAAATTCATTTAATAATAATAATTTAATTTTTTTCATAGTATTCAAAGATATAAT
GAAAAACTTTTATTATTTTATATTAATTTTATTATTTTTTAATGAAGTTTGCTATTCATT
AAAAGATGGTGAAATTAAACTTCATTTAATTCCACATTCACATTGTGATTCTGGATGGAC
ATCAACAATGAACGAATATTATATGGGTCAAGTTAAAAGTATTATATCATCAATGGTTCA
ATCATTAAATGTTGAATCAAATCCACCTAGAAAATTTGTTTGGTCTGAAATTGGATTTTT
AGAACAATGGTGGGATGATATGCCAATTGAAATTAAAAATGATTTTATAAAACATGTTAA
AAATGATAGAATTGAATTTGTAAATGGTGGATGGGTAATGAATGATGAAGCATGTGCAAG
TTTAGAAAGTGGTAATTAGACAATTATCAAATGGACATAAATTTATTAGAGAGAAATTTG
GAAAACAACCAGAGAGTGGATGGCAAATTGATCCATTTGGACATTCATCATTGACACCAA
CATTACAAGCTCAATTTGGTTATAAACATGTTGTATTGAATCGTATTCACTATGAATTGA
AAAAGAAATTTAAAGAAGAANNNNNNNNNN
Length of 5' end seq. 630
3' end seq. ID SHD168Z
3' end seq. 
>SHD168Z.Seq
NNNNNNNNNNGTTCNATTAAATTAAGAANTCATAATATTGATNNTAATAATCNATCTCCA
ATCNGTTTTGATTTANATGGCTNATTCTCTTTTATCAAATCAATTAAATCAATTAAAGAA
TANAATTTATNTNTAATCAATANATTCGTTGATAATAATTATTGATAATAGNATT
Length of 3' end seq. 175
Connected seq. ID SHD168P
Connected seq. 
>SHD168P.Seq
AAAATAAAATTCATTTAATAATAATAATTTAATTTTTTTCATAGTATTCAAAGATATAAT
GAAAAACTTTTATTATTTTATATTAATTTTATTATTTTTTAATGAAGTTTGCTATTCATT
AAAAGATGGTGAAATTAAACTTCATTTAATTCCACATTCACATTGTGATTCTGGATGGAC
ATCAACAATGAACGAATATTATATGGGTCAAGTTAAAAGTATTATATCATCAATGGTTCA
ATCATTAAATGTTGAATCAAATCCACCTAGAAAATTTGTTTGGTCTGAAATTGGATTTTT
AGAACAATGGTGGGATGATATGCCAATTGAAATTAAAAATGATTTTATAAAACATGTTAA
AAATGATAGAATTGAATTTGTAAATGGTGGATGGGTAATGAATGATGAAGCATGTGCAAG
TTTAGAAAGTGGTAATTAGACAATTATCAAATGGACATAAATTTATTAGAGAGAAATTTG
GAAAACAACCAGAGAGTGGATGGCAAATTGATCCATTTGGACATTCATCATTGACACCAA
CATTACAAGCTCAATTTGGTTATAAACATGTTGTATTGAATCGTATTCACTATGAATTGA
AAAAGAAATTTAAAGAAGAA----------GTTCNATTAAATTAAGAANTCATAATATTG
ATNNTAATAATCNATCTCCAATCNGTTTTGATTTANATGGCTNATTCTCTTTTATCAAAT
CAATTAAATCAATTAAAGAATANAATTTATNTNTAATCAATANATTCGTTGATAATAATT
ATTGATAATAGNATT
Length of connected seq. 785
Full length Seq ID -
Full length Seq. -
Length of full length seq. -