>SHD149 (SHD149Q) /CSM/SH/SHD1-C/SHD149Q.Seq.d/
XXXXXXXXXXTTCACCTGCTGTAAACTCTCATAAGATTGTAACTCAAATGGGAAATGTTG
AAAATAGTGAAATTACCAATGAACAAGATGCCATTGAACAAGCTCAACATTTACAACGTA
TTGAAAATGGTGAAGATATCGATGAGTATGAAGCTGCCCAACATGAAGTTGCCTTTTTAG
ATGAAAATGAACAAGATGCTGCTAAATTACAAAATGAAATGGACCAATTAGAACTCTTAC
TTGAAGAGAATGAAGAAGAACAATTTTCAAATAAAAATTTACAACAACTTAATGAGAATG
AAAATCAACAACAACAACAAGGACAACAACAAGTTCAAGCAACAAATGTTGAACAACAAA
TCGTTGAACAACTTAAAGTTATCAAAGAATTTCAAAGACAAGATCAACAAAAGAAACAAA
AAATTCAACAAGAGAACGATGCTGTCTACTTGAGTGATGTTGAAAAAGCTCAACTTGAAT
TGGATGCTGAATTGGCTAAAAATAATAAACAAGAGAATCAAGATGAGAATTTAGTTCAAG
AGAAACAACAATCTCCTGACCAAATTAAAAATCAANTAAAAAATAT

---SPAVNSHKIVTQMGNVENSEITNEQDAIEQAQHLQRIENGEDIDEYEAAQHEVAFLD
ENEQDAAKLQNEMDQLELLLEENEEEQFSNKNLQQLNENENQQQQQGQQQVQATNVEQQI
VEQLKVIKEFQRQDQQKKQKIQQENDAVYLSDVEKAQLELDAELAKNNKQENQDENLVQE
KQQSPDQIKNQXKN

Frame A:
---SPAVNSHKIVTQMGNVENSEITNEQDAIEQAQHLQRIENGEDIDEYEAAQHEVAFLD
ENEQDAAKLQNEMDQLELLLEENEEEQFSNKNLQQLNENENQQQQQGQQQVQATNVEQQI
VEQLKVIKEFQRQDQQKKQKIQQENDAVYLSDVEKAQLELDAELAKNNKQENQDENLVQE
KQQSPDQIKNQXKN


Frame B:
---hll*tlirl*lkwemlkivklpmnkmplnklniynvlkmvkismsmklpnmklpf*m
kmnkmllnykmkwtn*nsylkrmkknnfqikiynnlmrmkinnnnkdnnkfkqqmlnnks
lnnlklsknfkdkinkrnkkfnkrtmlst*vmlkklnlnwmlnwlkiinkrikmri*fkr
nnnlltklkix*ki


Frame C:
---tcckls*dcnsngkc*k**nyq*trch*tsstftty*kw*ryr*v*scpt*sclfr*
k*trcc*itk*ngpirtlt*re*rrtifk*kfttt**e*ksttttrtttsssnkc*ttnr
*tt*syqrisktrstketknstrercclle*c*ksst*igc*ig*k**tresr*efssre
ttis*pn*ksxkky

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHD149 (SHD149Q) /CSM/SH/SHD1-C/SHD149Q.Seq.d/                        583   e-165
VHP272 (VHP272Q) /CSM/VH/VHP2-C/VHP272Q.Seq.d/                        575   e-163
SHL894 (SHL894Q) /CSM/SH/SHL8-D/SHL894Q.Seq.d/                        575   e-163
SHL890 (SHL890Q) /CSM/SH/SHL8-D/SHL890Q.Seq.d/                        575   e-163
SHL879 (SHL879Q) /CSM/SH/SHL8-D/SHL879Q.Seq.d/                        575   e-163
SHL875 (SHL875Q) /CSM/SH/SHL8-D/SHL875Q.Seq.d/                        575   e-163
SHL866 (SHL866Q) /CSM/SH/SHL8-C/SHL866Q.Seq.d/                        575   e-163
SHL865 (SHL865Q) /CSM/SH/SHL8-C/SHL865Q.Seq.d/                        575   e-163
SHL857 (SHL857Q) /CSM/SH/SHL8-C/SHL857Q.Seq.d/                        575   e-163
SHL828 (SHL828Q) /CSM/SH/SHL8-B/SHL828Q.Seq.d/                        575   e-163

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U25143|U25143.1 Dictyostelium discoideum cAMP-inducible prespore protein gene, complete cds.   575   0.0    2
AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.   127   3e-25  1
DT633626|DT633626.1 EST1148557 Normalized pine embryo library, Lib_D Pinus taeda cDNA clone PIMFW90 3' end, mRNA sequence.    48   0.21   1
DT309975|DT309975.1 JGI_CAAX1517.fwd CAAX Pimephales promelas testis 7-8 month adults, males and females pooled (L) Pimephales promelas cDNA clone CAAX1517 5', mRNA sequence.    48   0.21   1
DT364098|DT364098.1 JGI_ANNN9278.rev ANNN Pimephales promelas Whole (L) Pimephales promelas cDNA clone ANNN9278 3', mRNA sequence.    48   0.21   1
DT309974|DT309974.1 JGI_CAAX1517.rev CAAX Pimephales promelas testis 7-8 month adults, males and females pooled (L) Pimephales promelas cDNA clone CAAX1517 3', mRNA sequence.    48   0.21   1
DT347978|DT347978.1 JGI_CAAX10802.fwd CAAX Pimephales promelas testis 7-8 month adults, males and females pooled (L) Pimephales promelas cDNA clone CAAX10802 5', mRNA sequence.    48   0.21   1
DR686823|DR686823.1 EST1076901 Normalized pine embryo library, Lib_D Pinus taeda cDNA clone PWABK24 3' end, mRNA sequence.    48   0.21   1
DT102344|DT102344.1 JGI_ANNN5669.rev ANNN Pimephales promelas Whole (L) Pimephales promelas cDNA clone ANNN5669 3', mRNA sequence.    48   0.21   1
CA966907|CA966907.2 CcLL10a35e10f1 Carp liver library 5 Cyprinus carpio cDNA clone 35e10 5' similar to zgc.nt BC059805, mRNA sequence.    48   0.21   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P54682) RecName: Full=cAMP-inducible prespore protein D7; Flags...   231   1e-59
(Q54F33) RecName: Full=Uncharacterized protein DDB_G0291143;           36   0.85
AF314648_2(AF314648|pid:none) Plasmodium vivax CG9 (cg9) gene, c...    35   1.1
AE016819_111(AE016819|pid:none) Ashbya gossypii (= Eremothecium ...    33   4.2
AC009323_10(AC009323|pid:none) Arabidopsis thaliana chromosome I...    33   5.5
AP009240_1649(AP009240|pid:none) Escherichia coli SE11 DNA, comp...    33   5.5
AY059831_1(AY059831|pid:none) Arabidopsis thaliana Unknown prote...    33   5.5
AE016796_1436(AE016796|pid:none) Vibrio vulnificus CMCP6 chromos...    33   7.2
AL844508_142(AL844508|pid:none) Plasmodium falciparum 3D7 chromo...    32   9.4
CP001078_829(CP001078|pid:none) Clostridium botulinum E3 str. Al...    32   9.4

 psg: 0.81  gvh: 0.16  alm: 0.58  top: 0.53  tms: 0.00  mit: 0.27  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 28.0 %: cytoplasmic
	 16.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane

>> prediction for SHD149 is nuc

>SHD149Z.Seq
NNNNNNNNNNTTCACCTGCTGTAAACTCTCATAAGATTGTAACTCAAATGGGAAATGTTG
AAAATAGTGAAATTACCAATGAACAAGATGCCATTGAACAAGCTCAACATTTACAACGTA
TTGAAAATGGTGAAGATATCGATGAGTATGAAGCTGCCCAACATGAAGTTGCCTTTTTAG
ATGAAAATGAACAAGATGCTGCTAAATTACAAAATGAAATGGACCAATTAGAACTCTTAC
TTGAAGAGAATGAAGAAGAACAATTTTCAAATAAAAATTTACAACAACTTAATGAGAATG
AAAATCAACAACAACAACAAGGACAACAACAAGTTCAAGCAACAAATGTTGAACAACAAA
TCGTTGAACAACTTAAAGTTATCAAAGAATTTCAAAGACAAGATCAACAAAAGAAACAAA
AAATTCAACAAGAGAACGATGCTGTCTACTTGAGTGATGTTGAAAAAGCTCAACTTGAAT
TGGATGCTGAATTGGCTAAAAATAATAAACAAGAGAATCAAGATGAGAATTTAGTTCAAG
AGAAACAACAATCTCCTGACCAAATTAAAAATCAANTAAAAAATAT