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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHD133
Library SH
(Link to library)
Clone ID SHD133
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13024-1|Contig-U16344-1
Original site URL
Representative seq. ID SHD133P
(Link to Original site)
Representative DNA sequence 
>SHD133 (SHD133Q) /CSM/SH/SHD1-B/SHD133Q.Seq.d/
ATTCAATCAACAAACAAAAAACAACAATTTAAAAAAACAATAATTTATAGTAAAGTAAAA
ACAATTAAAGAATCACTAATTGTTTTTTTCTCCTCCTCACTATAAATTCCAATATTTTTT
TTTAAATTAATTTCCAGCTTTTCTGTTTTTTTACACAATCACAACCAACCCTATATCATT
ACTACCAACCCTATACCATTACAATCTTATTTATTAAATAATTATTATTTGTTGTATTAT
TTTAGTGTATATTTGTTGATTTAATATTTATTAGCAAGTAACAAACAAAAAAAAAAXXXX
XXXXXXTCAAACAAGTGCTACTGCCGTCTTAAATATGGTCGCTGGTGGTTCAATTGAATC
AAAAGTTATTGATTTCACTCATGGTACCATTCAAGGTGTTGGTGCTCTTAAATGTGGTAC
TTGTAACTTTGCCAATAAGATCGCTGCTAACCTCAACATTGTTGGTAACGTTAAAATGGC
CGCAACTGCAACTGTCTATGCAAACATTGATGCTTTAATGTCAATCACCACCTCAACCCA
AACCTCAATCTCTCAATCATCAACCAAGACTGTCAACGGTAAGACCACCACTCAATCATC
ATCAAGCTCATCATCATCCATCTCTAAACAATTCGTTCCAATGATCGTTTGTTCAGGTAC
TGCTGATGTTGCTGGTAACCTCAATGTCGTTGCCTCAGAAGCCAATCAAGCTAAAATTGC
CTCTGGTACTGTATTCAAATTCATCTCAGCCCCAGTTGTTAAAGGTAATGTCTCCTCAGT
TAGCTGTCTCACCTCAAGCACTGCTGCTGCTGCTCAACAAGCTGCCGGTGCTGCTGCTGC
TGCTGCCCAAAAAGCTGCTATGGCTGCTGCCTCTAAAGTTTGGTCAATTGTAAATTCACC
ATGTAACTGTGAATGTGGATTGAAGAGATTATAAATTGATTCAACTCCTCCTCTCCAATG
ACAATAAATTAAAATAATACAACTCATTCTTATTTTTCTTTATTCTAACCAATGTATTTG
TCAATTAACTTATTAACTTTTTAAACACACCCTTTTCAATTTTAAAACAATAAAAATT
sequence update 2002.10.25
Translated Amino Acid sequence 
iqstnkkqqfkktiiyskvktikeslivffsssl*ipifffklissfsvflhnhnqpyii
ttnpiplqsyllnnyyllyyfsvylli*YLLASNKQKK---

---QTSATAVLNMVAGGSIESKVIDFTHGTIQGVGALKCGTCNFANKIAANLNIVGNVKM
AATATVYANIDALMSITTSTQTSISQSSTKTVNGKTTTQSSSSSSSSISKQFVPMIVCSG
TADVAGNLNVVASEANQAKIASGTVFKFISAPVVKGNVSSVSCLTSSTAAAAQQAAGAAA
AAAQKAAMAAASKVWSIVNSPCNCECGLKRL*idstpplq*q*ikiiqliliflysnqci
cqlty*lfkhtlfnfktiki
Translated Amino Acid sequence (All Frames) 
Frame A:
iqstnkkqqfkktiiyskvktikeslivffsssl*ipifffklissfsvflhnhnqpyii
ttnpiplqsyllnnyyllyyfsvylli*YLLASNKQKK---

---snkcycrlkygrwwfn*iksy*fhswyhsrcwcs*mwyl*lcq*drc*pqhcw*r*n
grncnclckh*cfnvnhhlnpnlnlsiinqdcqr*dhhsiiikliiihl*tirsndrlfr
yc*ccw*pqcrclrsqss*nclwyciqihlspsc*r*clls*lshlkhccccstscrccc
cccpkscygccl*slvnckftm*l*mwieeiin*fnssspmtin*nntthsyfslf*pmy
lsinlltf*thpfqf*nnkn

Frame B:
fnqqtknnnlkkq*fivk*kqlknh*lffspphykfqyffln*fpaflffytittnpisl
lptlyhynliy*iiiicciilvyic*fniy*qvtnkkk---

---QTSATAVLNMVAGGSIESKVIDFTHGTIQGVGALKCGTCNFANKIAANLNIVGNVKM
AATATVYANIDALMSITTSTQTSISQSSTKTVNGKTTTQSSSSSSSSISKQFVPMIVCSG
TADVAGNLNVVASEANQAKIASGTVFKFISAPVVKGNVSSVSCLTSSTAAAAQQAAGAAA
AAAQKAAMAAASKVWSIVNSPCNCECGLKRL*idstpplq*q*ikiiqliliflysnqci
cqlty*lfkhtlfnfktiki

Frame C:
sinkqktti*knnnl**sknn*ritncffllltinsniff*infqlfcfftqsqptlyhy
yqpytitilfik*llfvvlf*cifvdlifisk*qtkkk---

---kqvllps*iwslvvqlnqkllislmvpfkvlvllnvvlvtlpirslltstllvtlkw
pqlqlsmqtlml*cqsppqpkpqslnhqprlstvrpplnhhqahhhpslnnsfq*sfvqv
llmllvtsmslpqkpiklklplvlysnssqpqllkvmspqlavspqalllllnklpvlll
llpkkllwllplkfgql*ihhvtvnvd*rdykliqlllsndnklk*ynsflfffiltnvf
vn*linflntpfsilkq*k
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLA101 (SLA101Q) /CSM/SL/SLA1-A/SLA101Q.Seq.d/                        979   0.0
SHK762 (SHK762Q) /CSM/SH/SHK7-C/SHK762Q.Seq.d/                        979   0.0
SHG713 (SHG713Q) /CSM/SH/SHG7-A/SHG713Q.Seq.d/                        979   0.0
SHG514 (SHG514Q) /CSM/SH/SHG5-A/SHG514Q.Seq.d/                        979   0.0
SHG338 (SHG338Q) /CSM/SH/SHG3-B/SHG338Q.Seq.d/                        979   0.0
SHG152 (SHG152Q) /CSM/SH/SHG1-C/SHG152Q.Seq.d/                        979   0.0
SHE430 (SHE430Q) /CSM/SH/SHE4-B/SHE430Q.Seq.d/                        979   0.0
SHD645 (SHD645Q) /CSM/SH/SHD6-B/SHD645Q.Seq.d/                        979   0.0
SHD133 (SHD133Q) /CSM/SH/SHD1-B/SHD133Q.Seq.d/                        979   0.0
SHB618 (SHB618Q) /CSM/SH/SHB6-A/SHB618Q.Seq.d/                        979   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U83087|U83087.1 Dictyostelium discoideum rsc12 mRNA, partial cds.   979   0.0    2
AX344185|AX344185.1 Sequence 32 from Patent WO0200926.             40   0.085  4
AX251781|AX251781.1 Sequence 42 from Patent WO0168911.             40   0.085  4
BD460245|BD460245.1 Diagnosis of Diseases Associated with Cell Cycle.    40   0.085  4
BD452167|BD452167.1 Diagnosis of Diseases Associated with Cell Cycle.    40   0.085  4
AJ297856|AJ297856.1 Lucilia sericata partial bcd gene for bicoid protein.    50   0.10   1
AL845362|AL845362.4 Zebrafish DNA sequence from clone CH211-142H2 in linkage group 20.    38   0.33   3
AC156169|AC156169.2 Bos taurus clone CH240-50N13, *** SEQUENCING IN PROGRESS ***, 60 unordered pieces.    48   0.40   1
AC148223|AC148223.1 Colobus guereza clone CH272-19E23, WORKING DRAFT SEQUENCE, 4 ordered pieces.    48   0.40   1
AC127176|AC127176.3 Rattus norvegicus clone CH230-267P11, *** SEQUENCING IN PROGRESS ***, 1 ordered piece.    48   0.40   1
dna update 2006. 1.27
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U83087_1(U83087|pid:none) Dictyostelium discoideum rsc12 mRNA, p...   292   1e-77
AY530539_1(AY530539|pid:none) Mimulus ringens deficiens (DEF) mR...    34   9.5
protein update 2008.11.14
PSORT 

 psg: 0.87  gvh: 0.45  alm: 0.41  top: 0.60  tms: 0.00  mit: 0.28  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 36.0 %: nuclear
	 12.0 %: cytoskeletal
	 12.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for SHD133 is cyt
5' end seq. ID SHD133F
5' end seq. 
>SHD133F.Seq
ATTCAATCAACAAACAAAAAACAACAATTTAAAAAAACAATAATTTATAGTAAAGTAAAA
ACAATTAAAGAATCACTAATTGTTTTTTTCTCCTCCTCACTATAAATTCCAATATTTTTT
TTTAAATTAATTTCCAGCTTTTCTGTTTTTTTACACAATCACAACCAACCCTATATCATT
ACTACCAACCCTATACCATTACAATCTTATTTATTAAATAATTATTATTTGTTGTATTAT
TTTAGTGTATATTTGTTGATTTAATATTTATTAGCAAGTAACAAACAAAAAAAAAANNNN
NNNNNN
Length of 5' end seq. 306
3' end seq. ID SHD133Z
3' end seq. 
>SHD133Z.Seq
NNNNNNNNNNTCAAACAAGTGCTACTGCCGTCTTAAATATGGTCGCTGGTGGTTCAATTG
AATCAAAAGTTATTGATTTCACTCATGGTACCATTCAAGGTGTTGGTGCTCTTAAATGTG
GTACTTGTAACTTTGCCAATAAGATCGCTGCTAACCTCAACATTGTTGGTAACGTTAAAA
TGGCCGCAACTGCAACTGTCTATGCAAACATTGATGCTTTAATGTCAATCACCACCTCAA
CCCAAACCTCAATCTCTCAATCATCAACCAAGACTGTCAACGGTAAGACCACCACTCAAT
CATCATCAAGCTCATCATCATCCATCTCTAAACAATTCGTTCCAATGATCGTTTGTTCAG
GTACTGCTGATGTTGCTGGTAACCTCAATGTCGTTGCCTCAGAAGCCAATCAAGCTAAAA
TTGCCTCTGGTACTGTATTCAAATTCATCTCAGCCCCAGTTGTTAAAGGTAATGTCTCCT
CAGTTAGCTGTCTCACCTCAAGCACTGCTGCTGCTGCTCAACAAGCTGCCGGTGCTGCTG
CTGCTGCTGCCCAAAAAGCTGCTATGGCTGCTGCCTCTAAAGTTTGGTCAATTGTAAATT
CACCATGTAACTGTGAATGTGGATTGAAGAGATTATAAATTGATTCAACTCCTCCTCTCC
AATGACAATAAATTAAAATAATACAACTCATTCTTATTTTTCTTTATTCTAACCAATGTA
TTTGTCAATTAACTTATTAACTTTTTAAACACACCCTTTTCAATTTTAAAACAATAAAAA
TT
Length of 3' end seq. 782
Connected seq. ID SHD133P
Connected seq. 
>SHD133P.Seq
ATTCAATCAACAAACAAAAAACAACAATTTAAAAAAACAATAATTTATAGTAAAGTAAAA
ACAATTAAAGAATCACTAATTGTTTTTTTCTCCTCCTCACTATAAATTCCAATATTTTTT
TTTAAATTAATTTCCAGCTTTTCTGTTTTTTTACACAATCACAACCAACCCTATATCATT
ACTACCAACCCTATACCATTACAATCTTATTTATTAAATAATTATTATTTGTTGTATTAT
TTTAGTGTATATTTGTTGATTTAATATTTATTAGCAAGTAACAAACAAAAAAAAAA----
------TCAAACAAGTGCTACTGCCGTCTTAAATATGGTCGCTGGTGGTTCAATTGAATC
AAAAGTTATTGATTTCACTCATGGTACCATTCAAGGTGTTGGTGCTCTTAAATGTGGTAC
TTGTAACTTTGCCAATAAGATCGCTGCTAACCTCAACATTGTTGGTAACGTTAAAATGGC
CGCAACTGCAACTGTCTATGCAAACATTGATGCTTTAATGTCAATCACCACCTCAACCCA
AACCTCAATCTCTCAATCATCAACCAAGACTGTCAACGGTAAGACCACCACTCAATCATC
ATCAAGCTCATCATCATCCATCTCTAAACAATTCGTTCCAATGATCGTTTGTTCAGGTAC
TGCTGATGTTGCTGGTAACCTCAATGTCGTTGCCTCAGAAGCCAATCAAGCTAAAATTGC
CTCTGGTACTGTATTCAAATTCATCTCAGCCCCAGTTGTTAAAGGTAATGTCTCCTCAGT
TAGCTGTCTCACCTCAAGCACTGCTGCTGCTGCTCAACAAGCTGCCGGTGCTGCTGCTGC
TGCTGCCCAAAAAGCTGCTATGGCTGCTGCCTCTAAAGTTTGGTCAATTGTAAATTCACC
ATGTAACTGTGAATGTGGATTGAAGAGATTATAAATTGATTCAACTCCTCCTCTCCAATG
ACAATAAATTAAAATAATACAACTCATTCTTATTTTTCTTTATTCTAACCAATGTATTTG
TCAATTAACTTATTAACTTTTTAAACACACCCTTTTCAATTTTAAAACAATAAAAATT
Length of connected seq. 1068
Full length Seq ID -
Full length Seq. -
Length of full length seq. -