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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFJ117
Library AF
(Link to library)
Clone ID AFJ117
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U02819-1
Original site URL
Representative seq. ID AFJ117E
(Link to Original site)
Representative DNA sequence 
>AFJ117 (AFJ117Q) /CSM/AF/AFJ1-A/AFJ117Q.Seq.d/
CTAATGCAACAGGAACAACAGCAACTATAATTAAAAAGTTTGATAATATTTTATTTGATT
GTATATTTTATGGTTTTCCAATTCTCAGTCAAAGAGCAATAACAGATGGTATTATTGAAT
TAATGGAATATTCAAGTTTAGATAAAACTGCGGCAACATTAGAAGGTCATCCAAATTTTC
AATCTATACTTGAAATTTATAATTTAATTTCATCAGAAGTTTCAAAATGTTATCCACAAT
ATAATAGTTCACCTTCCACAACTACAACAAGTGTAAATAATAATAATGCGATAACAGTAA
ATGAAAATAGTAGTAGTAAAAATTCAACAGTTGTACCATTAGTTTTACAAACAATATCAC
CAACTACACCATCAAATATTAAACAAAATAATTTAAATTTATTTTTAAATCAAGAGAGAC
AACCCAATTGTATTGATTGTTGATAAGTTTTTACATTGTTTACCATTTGAATCGTTGGCA
CCATTGAATCAAATGTCATTGTATAGATTACCATCATTCACATTCCAGAGGTATCTTTGT
TATGATCAAATTTCAAATCAATTTAGTAAAGTTATGCCAATTATTGATCCAAAGAGTTTA
TATTATCTTTTGAACCCAAGTGGTGGCTTGAAAGAGACAGAGAATTACTTTGCACCTTAC
TTTAAAAAGAAATTTCCAGAATGGGATGGACTCGTTGGAAGTGTACCCTCGAAACATCAA
TATAAACAAGCGTTGGAGAATCATGATATTTTCTTTTACATGGGTCATGGTAGTGGTGAA
CAATACTTTAGAGGTGACCGTATTCAAAAGTTGGAAAAATGTGGTGTTTCTGTTTTAATG
GGTTGTAAAAGTGGTCATCTTGAAGAACAAGGTGAATTTGAACCAACTGGTGTTATACTC
GATTTCCTTTTGGCTGGTAGTAAAACTGTCATTGGTAATATCTATGATGTTCCAACCTCG
GATTGTGATCGTTTAACCAAATCTTTCTTAAACAAATGGTTTTTCGATTTAAAAACAAAT
GCTACAAAACCATTAACAATTGATATTAGTTTAGCAATTTCATTCGCTCGTAATCTTGT
sequence update 2001. 6. 9
Translated Amino Acid sequence 
lmqqeqqql*lksliifylivyfmvfqfsvkeq*qmvlln*wniqv*iklrqh*kviqif
nlylkfii*fhqkfqnvihniivhlpqlqqv*iiimr*q*mkivvvkiqqlyh*fykqyh
qlhhqilnkii*iyf*IKRDNPIVLIVDKFLHCLPFESLAPLNQMSLYRLPSFTFQRYLC
YDQISNQFSKVMPIIDPKSLYYLLNPSGGLKETENYFAPYFKKKFPEWDGLVGSVPSKHQ
YKQALENHDIFFYMGHGSGEQYFRGDRIQKLEKCGVSVLMGCKSGHLEEQGEFEPTGVIL
DFLLAGSKTVIGNIYDVPTSDCDRLTKSFLNKWFFDLKTNATKPLTIDISLAISFARNL
Translated Amino Acid sequence (All Frames) 
Frame A:
lmqqeqqql*lksliifylivyfmvfqfsvkeq*qmvlln*wniqv*iklrqh*kviqif
nlylkfii*fhqkfqnvihniivhlpqlqqv*iiimr*q*mkivvvkiqqlyh*fykqyh
qlhhqilnkii*iyf*IKRDNPIVLIVDKFLHCLPFESLAPLNQMSLYRLPSFTFQRYLC
YDQISNQFSKVMPIIDPKSLYYLLNPSGGLKETENYFAPYFKKKFPEWDGLVGSVPSKHQ
YKQALENHDIFFYMGHGSGEQYFRGDRIQKLEKCGVSVLMGCKSGHLEEQGEFEPTGVIL
DFLLAGSKTVIGNIYDVPTSDCDRLTKSFLNKWFFDLKTNATKPLTIDISLAISFARNL


Frame B:
*cnrnnsnyn*kv**yfi*lyilwfsnsqsksnnrwyy*ingifkfr*ncgnirrsskfs
iyt*nl*fnfirsfkmlsti**ftfhnynkck***cdnsk*k****kfnsctisftnnit
nytiky*tk*fkfifksrettqly*llisfyivyhlnrwhh*ikchcidyhhshsrgifv
mikfqinlvklcqlliqrvyiif*tqvva*krqritlhltlkrnfqngmdslevyprnin
inkrwrimifsftwvmvvvnntlevtvfkswknvvflf*wvvkvvilknkvnlnqlvlys
isfwlvvklslvismmfqpriviv*pnls*tngfsi*kqmlqnh*qlilv*qfhslvil


Frame C:
natgttatiikkfdnilfdcifygfpilsqraitdgiielmeyssldktaatleghpnfq
sileiynlissevskcypqynsspsttttsvnnnnaitvnensssknstvvplvlqtisp
ttpsnikqnnlnlflnqerqpncidc**vftlfti*ivgtiesnviv*itiihipevsll
*snfksi**syany*skefilsfepkwwlerdrellctll*keisrmgwtrwkctletsi
*tsvges*yfllhgsw*w*til*r*pyskvgkmwcfcfngl*kwss*rtr*i*tnwcytr
fpfgw**nchw*yl*csnlgl*sfnqiflkqmvfrfknkcyktinn*y*fsnfirs*sc
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFJ117 (AFJ117Q) /CSM/AF/AFJ1-A/AFJ117Q.Seq.d/                       2139   0.0
AHA814 (AHA814Q) /CSM/AH/AHA8-A/AHA814Q.Seq.d/                         42   0.017
SSH812 (SSH812Q) /CSM/SS/SSH8-A/SSH812Q.Seq.d/                         40   0.069
SSG406 (SSG406Q) /CSM/SS/SSG4-A/SSG406Q.Seq.d/                         40   0.069
SSE158 (SSE158Q) /CSM/SS/SSE1-C/SSE158Q.Seq.d/                         40   0.069
SSD848 (SSD848Q) /CSM/SS/SSD8-B/SSD848Q.Seq.d/                         40   0.069
SSD123 (SSD123Q) /CSM/SS/SSD1-A/SSD123Q.Seq.d/                         40   0.069
SSC396 (SSC396Q) /CSM/SS/SSC3-D/SSC396Q.Seq.d/                         40   0.069
SSC281 (SSC281Q) /CSM/SS/SSC2-D/SSC281Q.Seq.d/                         40   0.069
SSA888 (SSA888Q) /CSM/SS/SSA8-D/SSA888Q.Seq.d/                         40   0.069
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.    42   3e-11  21
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.    38   6e-11  21
AC116979|AC116979.3 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.    36   1e-10  25
AC116960|AC116960.2 Dictyostelium discoideum chromosome 2 map complement(1004496-821614) strain AX4, complete sequence.    36   3e-09  20
BX842642|BX842642.1 Mycoplasma mycoides subsp. mycoides SC genomic DNA, complete  sequence; segment 1/4.    32   9e-09  21
AE014817|AE014817.1 Plasmodium falciparum 3D7 chromosome 14 section 2 of 13 of the complete sequence.    34   1e-08  20
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.    36   3e-08  18
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    38   4e-08  21
AL844507|AL844507.1 Plasmodium falciparum chromosome 8.            42   4e-08  22
AL034557|AL034557.8 Plasmodium falciparum MAL4P1.                  36   4e-08  16
dna update 2004. 7.29
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

A42854(A42854;S27411)probable spindle pole body component bimB -...   142   2e-32
(P33144) RecName: Full=Separin;          EC=3.4.22.49; AltName: ...   142   2e-32
AP007151_231(AP007151|pid:none) Aspergillus oryzae RIB40 genomic...   141   3e-32
AM920427_583(AM920427|pid:none) Penicillium chrysogenum Wisconsi...   140   8e-32
AC157375_4(AC157375|pid:none) Medicago truncatula chromosome 2 c...   140   8e-32
M83232_1(M83232|pid:none) Aspergillus nidulans cell division-ass...   140   1e-31
A35694(A35694)cut1 protein - fission yeast (Schizosaccharomyces ...   138   3e-31
AM270126_31(AM270126|pid:none) Aspergillus niger contig An07c008...   138   4e-31
BC172238_1(BC172238|pid:none) Synthetic construct Homo sapiens c...   136   1e-30
AB179352_1(AB179352|pid:none) Macaca fascicularis testis cDNA cl...   136   1e-30
protein update 2009. 6.29
PSORT 

 psg: 0.75  gvh: 0.44  alm: 0.38  top: 0.53  tms: 0.00  mit: 0.23  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: cytoplasmic
	 32.0 %: nuclear
	  8.0 %: cytoskeletal

>> prediction for AFJ117 is cyt
5' end seq. ID AFJ117F
5' end seq. 
>AFJ117F.Seq
CTAATGCAACAGGAACAACAGCAACTATAATTAAAAAGTTTGATAATATTTTATTTGATT
GTATATTTTATGGTTTTCCAATTCTCAGTCAAAGAGCAATAACAGATGGTATTATTGAAT
TAATGGAATATTCAAGTTTAGATAAAACTGCGGCAACATTAGAAGGTCATCCAAATTTTC
AATCTATACTTGAAATTTATAATTTAATTTCATCAGAAGTTTCAAAATGTTATCCACAAT
ATAATAGTTCACCTTCCACAACTACAACAAGTGTAAATAATAATAATGCGATAACAGTAA
ATGAAAATAGTAGTAGTAAAAATTCAACAGTTGTACCATTAGTTTTACAAACAATATCAC
CAACTACACCATCAAATATTAAACAAAATAATTTAAATTTATTTTTAAATCAAGAGAGAC
AACCAATTGTATTGATTGTTGATAAGTTTTTACATTGTTTACCATTTGAATCGTTGGCAC
CATTGAATCAAATGTCATTGTATAGATTACCATCATTCACATTCCAGAGGTATCTTTGTT
ATGATCAAATTTCAAATCAATTTAGTA----------
Length of 5' end seq. 567
3' end seq. ID AFJ117Z
3' end seq. 
>AFJ117Z.Seq
----------TAAACAAAATAATTTAAATTTATTTTTAAATCAAGAGAGACAACCCAATT
GTATTGATTGTTGATAAGTTTTTACATTGTTTACCATTTGAATCGTTGGCACCATTGAAT
CAAATGTCATTGTATAGATTACCATCATTCACATTCCAGAGGTATCTTTGTTATGATCAA
ATTTCAAATCAATTTAGTAAAGTTATGCCAATTATTGATCCAAAGAGTTTATATTATCTT
TTGAACCCAAGTGGTGGCTTGAAAGAGACAGAGAATTACTTTGCACCTTACTTTAAAAAG
AAATTTCCAGAATGGGATGGACTCGTTGGAAGTGTACCCTCGAAACATCAATATAAACAA
GCGTTGGAGAATCATGATATTTTCTTTTACATGGGTCATGGTAGTGGTGAACAATACTTT
AGAGGTGACCGTATTCAAAAGTTGGAAAAATGTGGTGTTTCTGTTTTAATGGGTTGTAAA
AGTGGTCATCTTGAAGAACAAGGTGAATTTGAACCAACTGGTGTTATACTCGATTTCCTT
TTGGCTGGTAGTAAAACTGTCATTGGTAATATCTATGATGTTCCAACCTCGGATTGTGAT
CGTTTAACCAAATCTTTCTTAAACAAATGGTTTTTCGATTTAAAAACAAATGCTACAAAA
CCATTAACAATTGATATTAGTTTAGCAATTTCATTCGCTCGTAATCTTGT
Length of 3' end seq. 700
Connected seq. ID AFJ117P
Connected seq. 
>AFJ117P.Seq
CTAATGCAACAGGAACAACAGCAACTATAATTAAAAAGTTTGATAATATTTTATTTGATT
GTATATTTTATGGTTTTCCAATTCTCAGTCAAAGAGCAATAACAGATGGTATTATTGAAT
TAATGGAATATTCAAGTTTAGATAAAACTGCGGCAACATTAGAAGGTCATCCAAATTTTC
AATCTATACTTGAAATTTATAATTTAATTTCATCAGAAGTTTCAAAATGTTATCCACAAT
ATAATAGTTCACCTTCCACAACTACAACAAGTGTAAATAATAATAATGCGATAACAGTAA
ATGAAAATAGTAGTAGTAAAAATTCAACAGTTGTACCATTAGTTTTACAAACAATATCAC
CAACTACACCATCAAATATTAAACAAAATAATTTAAATTTATTTTTAAATCAAGAGAGAC
AACCAATTGTATTGATTGTTGATAAGTTTTTACATTGTTTACCATTTGAATCGTTGGCAC
CATTGAATCAAATGTCATTGTATAGATTACCATCATTCACATTCCAGAGGTATCTTTGTT
ATGATCAAATTTCAAATCAATTTAGTA----------TAAACAAAATAATTTAAATTTAT
TTTTAAATCAAGAGAGACAACCCAATTGTATTGATTGTTGATAAGTTTTTACATTGTTTA
CCATTTGAATCGTTGGCACCATTGAATCAAATGTCATTGTATAGATTACCATCATTCACA
TTCCAGAGGTATCTTTGTTATGATCAAATTTCAAATCAATTTAGTAAAGTTATGCCAATT
ATTGATCCAAAGAGTTTATATTATCTTTTGAACCCAAGTGGTGGCTTGAAAGAGACAGAG
AATTACTTTGCACCTTACTTTAAAAAGAAATTTCCAGAATGGGATGGACTCGTTGGAAGT
GTACCCTCGAAACATCAATATAAACAAGCGTTGGAGAATCATGATATTTTCTTTTACATG
GGTCATGGTAGTGGTGAACAATACTTTAGAGGTGACCGTATTCAAAAGTTGGAAAAATGT
GGTGTTTCTGTTTTAATGGGTTGTAAAAGTGGTCATCTTGAAGAACAAGGTGAATTTGAA
CCAACTGGTGTTATACTCGATTTCCTTTTGGCTGGTAGTAAAACTGTCATTGGTAATATC
TATGATGTTCCAACCTCGGATTGTGATCGTTTAACCAAATCTTTCTTAAACAAATGGTTT
TTCGATTTAAAAACAAATGCTACAAAACCATTAACAATTGATATTAGTTTAGCAATTTCA
TTCGCTCGTAATCTTGT
Length of connected seq. 1267
Full length Seq ID AFJ117E
Full length Seq. 
>AFJ117E.Seq
CTAATGCAACAGGAACAACAGCAACTATAATTAAAAAGTTTGATAATATTTTATTTGATT
GTATATTTTATGGTTTTCCAATTCTCAGTCAAAGAGCAATAACAGATGGTATTATTGAAT
TAATGGAATATTCAAGTTTAGATAAAACTGCGGCAACATTAGAAGGTCATCCAAATTTTC
AATCTATACTTGAAATTTATAATTTAATTTCATCAGAAGTTTCAAAATGTTATCCACAAT
ATAATAGTTCACCTTCCACAACTACAACAAGTGTAAATAATAATAATGCGATAACAGTAA
ATGAAAATAGTAGTAGTAAAAATTCAACAGTTGTACCATTAGTTTTACAAACAATATCAC
CAACTACACCATCAAATATTAAACAAAATAATTTAAATTTATTTTTAAATCAAGAGAGAC
AACCCAATTGTATTGATTGTTGATAAGTTTTTACATTGTTTACCATTTGAATCGTTGGCA
CCATTGAATCAAATGTCATTGTATAGATTACCATCATTCACATTCCAGAGGTATCTTTGT
TATGATCAAATTTCAAATCAATTTAGTAAAGTTATGCCAATTATTGATCCAAAGAGTTTA
TATTATCTTTTGAACCCAAGTGGTGGCTTGAAAGAGACAGAGAATTACTTTGCACCTTAC
TTTAAAAAGAAATTTCCAGAATGGGATGGACTCGTTGGAAGTGTACCCTCGAAACATCAA
TATAAACAAGCGTTGGAGAATCATGATATTTTCTTTTACATGGGTCATGGTAGTGGTGAA
CAATACTTTAGAGGTGACCGTATTCAAAAGTTGGAAAAATGTGGTGTTTCTGTTTTAATG
GGTTGTAAAAGTGGTCATCTTGAAGAACAAGGTGAATTTGAACCAACTGGTGTTATACTC
GATTTCCTTTTGGCTGGTAGTAAAACTGTCATTGGTAATATCTATGATGTTCCAACCTCG
GATTGTGATCGTTTAACCAAATCTTTCTTAAACAAATGGTTTTTCGATTTAAAAACAAAT
GCTACAAAACCATTAACAATTGATATTAGTTTAGCAATTTCATTCGCTCGTAATCTTGT
Length of full length seq. 1079