>SHC825 (SHC825Q) /CSM/SH/SHC8-B/SHC825Q.Seq.d/
TGGCCTACTGGNAAAAATGTCAAATAAATAAAAAAAAAAAAAAAAATTAAAAAAAATTTT
ANTANGTTTTAAAAGGGNNTAGTTTTAATAAAAAAAAAAAAAANCCCCNTTTTTTTTTTN
NTAAAAAAAAANNAAAAANNAAAAAAAAAAAAANAAAAAAAAANNNNNNNNNCCNNNCCC
CGGGGGCNTTAAAAAAAAAAAAAGNTTTNAAAAAAAAANAAAAAANNNNNAAAAAAAAAA
AAAAAANNAAAAAAAAANCNTTTTAAANCCCCCNNNNNNNTTTTGGGGGNCNTNNNAAAN
NNAANNNNNGGGGGGNNNNNCCCCCCNTNNNTTNNNNNNNCNCCCCCCNNCCCNAAAAAN
TTTGGGCCCCCCNAAAANNNGCCCCCAAANNTTNCCNGGGGGGAANCCCCNNNNTTNNTT
NGNNCCNTTTTNAANNGNNAAACCCCNTTTTNNNNAAAAAAANTTTTTTTAAAAAAAAAA
ANNNAAAAAANNNNTTTTTTAAANTTTTTTTTTNAAANNCCTTTTAAAAAAAAAAXXXXX
XXXXX

gllxkmsnk*kkkkklkkilxxfkrx*f**kkkkxpfffx*KKXKXXKKKXKKKXXXPXP
GGXKKKKXFXKKXKXXXKKKKXXKKXXLXPPXXFWGXXXXXXXGGXXPXXXXXXPPXXKX
LGPPKXXPQXXPGGXPXXXXXPFXXXKPXFXXKXFF*kkkxkkxxf*xffxkxllkk---

Frame A:
wptgknvk*ikkkkkikknfxxf*kgxvlikkkkxpxffxxkkxxkxkkkkxkkxxxxxp
rgx*kkkkxxkkxkkxxkkkkkxkkxxfkxpxxxlgxxxxxxxggxxppxxxxxppxpkx
fgppxxxppxxxxgexpxxxxxxxxxxtpfxxkkxflkkkxxkxxflxfffxxpfkkk--
-


Frame B:
gllxkmsnk*kkkkklkkilxxfkrx*f**kkkkxpfffx*KKXKXXKKKXKKKXXXPXP
GGXKKKKXFXKKXKXXXKKKKXXKKXXLXPPXXFWGXXXXXXXGGXXPXXXXXXPPXXKX
LGPPKXXPQXXPGGXPXXXXXPFXXXKPXFXXKXFF*kkkxkkxxf*xffxkxllkk---


Frame C:
aywxkcqinkkkkkn*kkfxxvlkgxsfnkkkkxpxfffxkkkxkxkkkkxkkxxxxxxp
galkkkkxxkkkxkxxkkkkkxkkkxf*xpxxxfggxxkxxxxgxxppxxxxxxpxpxkx
wapxkxapkxxxggxpxxxxxxfxxxnpxxxkkxffkkkxxkxxffkxffxxxf*kk---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHJ712 (SHJ712Q) /CSM/SH/SHJ7-A/SHJ712Q.Seq.d/                         52   9e-06
SHJ610 (SHJ610Q) /CSM/SH/SHJ6-A/SHJ610Q.Seq.d/                         52   9e-06
SHH756 (SHH756Q) /CSM/SH/SHH7-C/SHH756Q.Seq.d/                         52   9e-06
SHH184 (SHH184Q) /CSM/SH/SHH1-D/SHH184Q.Seq.d/                         52   9e-06
SHE893 (SHE893Q) /CSM/SH/SHE8-D/SHE893Q.Seq.d/                         52   9e-06
SHC825 (SHC825Q) /CSM/SH/SHC8-B/SHC825Q.Seq.d/                         52   9e-06
SHB275 (SHB275Q) /CSM/SH/SHB2-D/SHB275Q.Seq.d/                         52   9e-06
SFJ322 (SFJ322Q) /CSM/SF/SFJ3-A/SFJ322Q.Seq.d/                         52   9e-06
CHM527 (CHM527Q) /CSM/CH/CHM5-B/CHM527Q.Seq.d/                         52   9e-06
AHM656 (AHM656Q) /CSM/AH/AHM6-C/AHM656Q.Seq.d/                         52   9e-06

 ***** No hits found ******

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Hits to DB: 873,108
Number of Sequences: 52510890
Number of extensions: 873108
Number of successful extensions: 56854
Number of sequences better than 10.0: 0
length of query: 545
length of database: 56,384,190,912
effective HSP length: 23
effective length of query: 522
effective length of database: 55,176,440,442
effective search space: 28802101910724
effective search space used: 28802101910724
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 21 (42.1 bits)

ANTI-DNA BLAST search was processed by blast@nig.ac.jp, National Institute of Genetics, Japan.

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BT051012_1(BT051012|pid:none) Medicago truncatula clone MTYFD_FE...    52   9e-06
BT050756_1(BT050756|pid:none) Medicago truncatula clone MTYF5_F6...    52   9e-06
AX647645_1(AX647645|pid:none) Sequence 1837 from Patent EP1270724.     50   5e-05
AY811874_1(AY811874|pid:none) Schistosoma japonicum SJCHGC01957 ...    47   2e-04
AM433463_1(AM433463|pid:none) Vitis vinifera contig VV78X022531....    47   4e-04
AY812022_1(AY812022|pid:none) Schistosoma japonicum clone SJCHGC...    45   9e-04
BT051163_1(BT051163|pid:none) Medicago truncatula clone MTYFT1G-...    44   0.002
BC027831_1(BC027831|pid:none) Mus musculus papillary renal cell ...    44   0.002
BT050894_1(BT050894|pid:none) Medicago truncatula clone MTYF9_FA...    44   0.003
AM269894_60(AM269894|pid:none) Eimeria tenella chromosome 1, ord...    44   0.003

 psg: 0.57  gvh: 0.47  alm: 0.63  top: 0.53  tms: 0.00  mit: 0.22  mip: 0.00
 nuc: 0.62  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 84.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system
	  4.0 %: cytoplasmic

>> prediction for SHC825 is nuc

>SHC825F.Seq
TGGCCTACTGGNAAAAATGTCAAATAAATAAAAAAAAAAAAAAAAATTAAAAAAAATTTT
ANTANGTTTTAAAAGGGNNTAGTTTTAATAAAAAAAAAAAAAANCCCCNTTTTTTTTTTN
NTAAAAAAAAANNAAAAANNAAAAAAAAAAAAANAAAAAAAAANNNNNNNNNCCNNNCCC
CGGGGGCNTTAAAAAAAAAAAAAGNTTTNAAAAAAAAANAAAAAANNNNNAAAAAAAAAA
AAAAAANNAAAAAAAAANCNTTTTAAANCCCCCNNNNNNNTTTTGGGGGNCNTNNNAAAN
NNAANNNNNGGGGGGNNNNNCCCCCCNTNNNTTNNNNNNNCNCCCCCCNNCCCNAAAAAN
TTTGGGCCCCCCNAAAANNNGCCCCCAAANNTTNCCNGGGGGGAANCCCCNNNNTTNNTT
NGNNCCNTTTTNAANNGNNAAACCCCNTTTTNNNNAAAAAAANTTTTTTTAAAAAAAAAA
ANNNAAAAAANNNNTTTTTTAAANTTTTTTTTTNAAANNCCTTTTAAAAAAAAAANNNNN
NNNNN