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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFJ111
Library AF
(Link to library)
Clone ID AFJ111
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U08806-1
Original site URL
Representative seq. ID AFJ111P
(Link to Original site)
Representative DNA sequence 
>AFJ111 (AFJ111Q) /CSM/AF/AFJ1-A/AFJ111Q.Seq.d/
TTCAGAAGCTGGTAGAACCACATTTAGAACCATCATTAAATCATTGGAAATTCATCCATT
CATTAGTGTACCATTATTGGTTGATTTTATACCAGAGAAACATGTTGCTGTCTCTGTTAG
ACCTTTCTATGCCGATAGAGGTTCATTAAGAGATTTCATACATAAATCAAAACCAAAAAT
GCCATACGCTGATAAATATGATACTCATCTTCAATTAAATGAAAAAATAGTTTCAAAATT
TGGTAGACAAATTTTAGAAGCTTTAATATTTTTAAAAAATCATAATTTCCCTTATTTTCA
TTTAAATTCTGCAAATGTTTTAGTTGATGATCAAATTTGTTTAATAAGTGATTATGAAAA
TTCATTTTTAGGATTAGAACCAAGATTTTCAGATTTTATTAGACAACATAATGAAAAGAT
TGATCCAGATGTATTATCATTTGGTTTAGTATTATTTGAGATGGCATGTGGTTATGAGAT
GGAGAATCCACATTCAGTTGATATCTCTATTCCAGCACATTGCTATCCAGAAGTTAGAAA
AGTATTAGAAGCAATTTTTAAACCATTCTATGGTACACCAATTACATTGGAAGXXXXXXX
XXXCNAGTAGTAGNTTNAATAGNAGTNNCAATAGNAACGTTTCNNCNTCANANNCNAATG
CANCAACCACNACNAANACCACTTCTGCATCAAGTGTTTCACCACCAATTTNTTCACCAC
CTCCACCTCCACCTCCACCACCACCATCCAAATCTTCAGGACCACCACCTCCACCACCAC
CACCACCCAAATCTTCAGGTCCTCCACCTCCACCACCACCCAAATCCTCCCCACCACCAC
CAGCTGATGGCAGTAGAAAAGGTTTACTATCCTCAATTGAATCTTTTTCTTCTTCAAAAT
TAAAAAAAACAAAAACTGTNATAAAAGTNGTCCGTATTAAAAAAATCTTAAACAAAACAA
AACAAAACAAAACAAAAAAAAGAAAACAAAACAAAAAANAAAAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
SEAGRTTFRTIIKSLEIHPFISVPLLVDFIPEKHVAVSVRPFYADRGSLRDFIHKSKPKM
PYADKYDTHLQLNEKIVSKFGRQILEALIFLKNHNFPYFHLNSANVLVDDQICLISDYEN
SFLGLEPRFSDFIRQHNEKIDPDVLSFGLVLFEMACGYEMENPHSVDISIPAHCYPEVRK
VLEAIFKPFYGTPITLE---

---SSXXNXSXNXNVSXSXXNAXTTTXTTSASSVSPPIXSPPPPPPPPPPSKSSGPPPPP
PPPPKSSGPPPPPPPKSSPPPPADGSRKGLLSSIESFSSSKLKKTKTVIKVVRIKKILNK
TKQNKTKKRKQNKKXK
Translated Amino Acid sequence (All Frames) 
Frame A:
frsw*nhi*nhh*iignssih*ctiig*fytretccclc*tflcr*rfikrfht*iktkn
air**i*ysssik*knsfkiw*tnfrsfnifkks*fplfsfkfckcfs**snlfnk*l*k
fifrirtkifrfy*tt**kd*srciiiwfsii*dgmwl*dgestfs*ylysstllsrs*k
sirsnf*tilwytnyig---

---xvvxxixvxixtfxxxxxmxqpxxxpllhqvfhhqfxhhlhlhlhhhhpnlqdhhlh
hhhhpnlqvlhlhhhpnpphhhqlmavekvyypqlnlfllqn*kkqklx*kxsvlkks*t
kqnktkqkkenktkxk

Frame B:
SEAGRTTFRTIIKSLEIHPFISVPLLVDFIPEKHVAVSVRPFYADRGSLRDFIHKSKPKM
PYADKYDTHLQLNEKIVSKFGRQILEALIFLKNHNFPYFHLNSANVLVDDQICLISDYEN
SFLGLEPRFSDFIRQHNEKIDPDVLSFGLVLFEMACGYEMENPHSVDISIPAHCYPEVRK
VLEAIFKPFYGTPITLE---

---x**xx*xxq*xrfxxxxxcxnhxxxhfcikcfttnxfttstststttiqifrtttst
ttttqifrsststttqilpttts*wq*krftiln*iffffkikknkncxksxpy*knlkq
nktkqnkkkktkqkxk

Frame C:
qklvephlepslnhwkfihslvyhywlilyqrnmllslldlsmpievh*eisyinqnqkc
htlinmilifn*mkk*fqnlvdkf*kl*yf*kiiislifi*ilqmf*lmikfv**vimki
hf*d*nqdfqilldnimkrliqmyyhlv*yylrwhvvmrwrihiqlislfqhiaiqklek
y*kqflnhsmvhqlhwk---

---SSXXNXSXNXNVSXSXXNAXTTTXTTSASSVSPPIXSPPPPPPPPPPSKSSGPPPPP
PPPPKSSGPPPPPPPKSSPPPPADGSRKGLLSSIESFSSSKLKKTKTVIKVVRIKKILNK
TKQNKTKKRKQNKKXK
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFJ111 (AFJ111Q) /CSM/AF/AFJ1-A/AFJ111Q.Seq.d/                       1243   0.0
VFH761 (VFH761Q) /CSM/VF/VFH7-C/VFH761Q.Seq.d/                        529   e-149
VSB379 (VSB379Q) /CSM/VS/VSB3-D/VSB379Q.Seq.d/                        109   8e-23
VSB473 (VSB473Q) /CSM/VS/VSB4-D/VSB473Q.Seq.d/                        105   1e-21
VSJ411 (VSJ411Q) /CSM/VS/VSJ4-A/VSJ411Q.Seq.d/                         42   0.016
VSC645 (VSC645Q) /CSM/VS/VSC6-B/VSC645Q.Seq.d/                         38   0.25
SSK706 (SSK706Q) /CSM/SS/SSK7-A/SSK706Q.Seq.d/                         38   0.25
SSJ843 (SSJ843Q) /CSM/SS/SSJ8-B/SSJ843Q.Seq.d/                         38   0.25
SSG670 (SSG670Q) /CSM/SS/SSG6-C/SSG670Q.Seq.d/                         38   0.25
SSF395 (SSF395Q) /CSM/SS/SSF3-D/SSF395Q.Seq.d/                         38   0.25
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    38   4e-05  20
BX957219|BX957219.1 Methanococcus maripaludis S2 complete genome; segment 1/5.    38   5e-05  12
AP003192|AP003192.2 Clostridium perfringens str. 13 DNA, complete genome, section 8/10.    32   2e-04  15
AL844509|AL844509.1 Plasmodium falciparum chromosome 13.           34   5e-04  24
AC116963|AC116963.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.    30   8e-04  15
AF250284|AF250284.1 Amsacta moorei entomopoxvirus, complete genome.    36   0.002  15
AE017263|AE017263.1 Mesoplasma florum L1 complete genome.          32   0.002  24
BX842643|BX842643.1 Mycoplasma mycoides subsp. mycoides SC genomic DNA, complete  sequence; segment 2/4.    32   0.003  14
BX842642|BX842642.1 Mycoplasma mycoides subsp. mycoides SC genomic DNA, complete  sequence; segment 1/4.    34   0.003  14
AE014843|AE014843.1 Plasmodium falciparum 3D7 chromosome 11 section 8 of 8 of the complete sequence.    34   0.010  12
dna update 2004. 9.24
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54WZ5) RecName: Full=Probable inactive serine/threonine-protei...   404   e-111
DQ095196_1(DQ095196|pid:none) Mus musculus modulator of Na,K-ATP...    92   3e-17
AK150337_1(AK150337|pid:none) Mus musculus bone marrow macrophag...    92   3e-17
(Q91WB6) RecName: Full=PX domain-containing protein kinase-like ...    92   3e-17
BX649457_1(BX649457|pid:none) Zebrafish DNA sequence from clone ...    92   3e-17
AK152058_1(AK152058|pid:none) Mus musculus bone marrow macrophag...    91   5e-17
AK157364_1(AK157364|pid:none) Mus musculus activated spleen cDNA...    91   6e-17
AE014134_1362(AE014134|pid:none) Drosophila melanogaster chromos...    91   6e-17
CR859845_1(CR859845|pid:none) Pongo abelii mRNA; cDNA DKFZp469B2...    90   1e-16
AB209688_1(AB209688|pid:none) Homo sapiens mRNA for PX domain-co...    89   2e-16
protein update 2009. 4.23
PSORT 

 psg: 0.72  gvh: 0.33  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.34  mip: 0.03
 nuc: 0.12  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 28.0 %: cytoplasmic
	 20.0 %: mitochondrial
	  8.0 %: peroxisomal

>> prediction for AFJ111 is nuc
5' end seq. ID AFJ111F
5' end seq. 
>AFJ111F.Seq
TTCAGAAGCTGGTAGAACCACATTTAGAACCATCATTAAATCATTGGAAATTCATCCATT
CATTAGTGTACCATTATTGGTTGATTTTATACCAGAGAAACATGTTGCTGTCTCTGTTAG
ACCTTTCTATGCCGATAGAGGTTCATTAAGAGATTTCATACATAAATCAAAACCAAAAAT
GCCATACGCTGATAAATATGATACTCATCTTCAATTAAATGAAAAAATAGTTTCAAAATT
TGGTAGACAAATTTTAGAAGCTTTAATATTTTTAAAAAATCATAATTTCCCTTATTTTCA
TTTAAATTCTGCAAATGTTTTAGTTGATGATCAAATTTGTTTAATAAGTGATTATGAAAA
TTCATTTTTAGGATTAGAACCAAGATTTTCAGATTTTATTAGACAACATAATGAAAAGAT
TGATCCAGATGTATTATCATTTGGTTTAGTATTATTTGAGATGGCATGTGGTTATGAGAT
GGAGAATCCACATTCAGTTGATATCTCTATTCCAGCACATTGCTATCCAGAAGTTAGAAA
AGTATTAGAAGCAATTTTTAAACCATTCTATGGTACACCAATTACATTGGAAG-------
---
Length of 5' end seq. 593
3' end seq. ID AFJ111Z
3' end seq. 
>AFJ111Z.Seq
----------CNAGTAGTAGNTTNAATAGNAGTNNCAATAGNAACGTTTCNNCNTCANAN
NCNAATGCANCAACCACNACNAANACCACTTCTGCATCAAGTGTTTCACCACCAATTTNT
TCACCACCTCCACCTCCACCTCCACCACCACCATCCAAATCTTCAGGACCACCACCTCCA
CCACCACCACCACCCAAATCTTCAGGTCCTCCACCTCCACCACCACCCAAATCCTCCCCA
CCACCACCAGCTGATGGCAGTAGAAAAGGTTTACTATCCTCAATTGAATCTTTTTCTTCT
TCAAAATTAAAAAAAACAAAAACTGTNATAAAAGTNGTCCGTATTAAAAAAATCTTAAAC
AAAACAAAACAAAACAAAACAAAAAAAAGAAAACAAAACAAAAAANAAAAA
Length of 3' end seq. 401
Connected seq. ID AFJ111P
Connected seq. 
>AFJ111P.Seq
TTCAGAAGCTGGTAGAACCACATTTAGAACCATCATTAAATCATTGGAAATTCATCCATT
CATTAGTGTACCATTATTGGTTGATTTTATACCAGAGAAACATGTTGCTGTCTCTGTTAG
ACCTTTCTATGCCGATAGAGGTTCATTAAGAGATTTCATACATAAATCAAAACCAAAAAT
GCCATACGCTGATAAATATGATACTCATCTTCAATTAAATGAAAAAATAGTTTCAAAATT
TGGTAGACAAATTTTAGAAGCTTTAATATTTTTAAAAAATCATAATTTCCCTTATTTTCA
TTTAAATTCTGCAAATGTTTTAGTTGATGATCAAATTTGTTTAATAAGTGATTATGAAAA
TTCATTTTTAGGATTAGAACCAAGATTTTCAGATTTTATTAGACAACATAATGAAAAGAT
TGATCCAGATGTATTATCATTTGGTTTAGTATTATTTGAGATGGCATGTGGTTATGAGAT
GGAGAATCCACATTCAGTTGATATCTCTATTCCAGCACATTGCTATCCAGAAGTTAGAAA
AGTATTAGAAGCAATTTTTAAACCATTCTATGGTACACCAATTACATTGGAAG-------
---CNAGTAGTAGNTTNAATAGNAGTNNCAATAGNAACGTTTCNNCNTCANANNCNAATG
CANCAACCACNACNAANACCACTTCTGCATCAAGTGTTTCACCACCAATTTNTTCACCAC
CTCCACCTCCACCTCCACCACCACCATCCAAATCTTCAGGACCACCACCTCCACCACCAC
CACCACCCAAATCTTCAGGTCCTCCACCTCCACCACCACCCAAATCCTCCCCACCACCAC
CAGCTGATGGCAGTAGAAAAGGTTTACTATCCTCAATTGAATCTTTTTCTTCTTCAAAAT
TAAAAAAAACAAAAACTGTNATAAAAGTNGTCCGTATTAAAAAAATCTTAAACAAAACAA
AACAAAACAAAACAAAAAAAAGAAAACAAAACAAAAAANAAAAA
Length of connected seq. 994
Full length Seq ID -
Full length Seq. -
Length of full length seq. -