>AFI894 (AFI894Q) /CSM/AF/AFI8-D/AFI894Q.Seq.d/
XXXXXXXXXXTGGAAAACCTCTATCAATTATTCATCAAGAGTGATGCAACTCAAGTCGAG
ATTAACCCATTCGCTGAAACCACCGATGGTCAAGTCATTTGTATGGATGCTAAAATCAAT
TTCGATGACAATGCCTCATTCCGTCAAAAGGAGATTTTCGAAATGAGAGATACCGCCGAA
GAGGATCCACGTGAGGTTGAAGCTGGTAAATTTGGTCTCAATTACATTGGTCTCGATGGT
AACATTGGTTGTATGGTTAATGGTGCTGGTTTGGCTATGGCCACCATGGATATCATTAAA
TTAAAGGGTGGTATCCCTGCTAATTTCCTCGATGTAGGTGGTAGTGCCTCTGAACAAGCC
GTCACTGAAGCCTTCAAAATCCTCACTAAAGATCCAAGAGTTAAATGTTTACTCGTAAAC
ATTTTCGGTGGTATCATNNAATGTGATATCATTGCCTCTGGTATCGTAAATGCTTCAAAA
CAAGTTGGTCTTAAAATTCCATTAGTTGTACGTTTAGAAGGTACAAATGTAAACATTGGT
AAAGAAATCTTGGAAAAATCTGGTTTAAATATTACTTCTGCTAGAGATTTAGATGATGCT
GCTATNAAAAAC

---ENLYQLFIKSDATQVEINPFAETTDGQVICMDAKINFDDNASFRQKEIFEMRDTAEE
DPREVEAGKFGLNYIGLDGNIGCMVNGAGLAMATMDIIKLKGGIPANFLDVGGSASEQAV
TEAFKILTKDPRVKCLLVNIFGGIXXCDIIASGIVNASKQVGLKIPLVVRLEGTNVNIGK
EILEKSGLNITSARDLDDAAXKN

Frame A:
---gkplsiihqe*cnssrd*pir*nhrwsshlygc*nqfr*qclipskgdfrneryrrr
gst*g*sw*iwsqlhwsrw*hwlyg*wcwfgyghhgyh*ikgwypc*fprcrw*cl*tsr
h*slqnph*rsks*mftrkhfrwyhxm*yhclwyrkcfktsws*nsisctfrrykckhw*
rnlgkiwfkyyfc*rfr*ccyxk


Frame B:
---ENLYQLFIKSDATQVEINPFAETTDGQVICMDAKINFDDNASFRQKEIFEMRDTAEE
DPREVEAGKFGLNYIGLDGNIGCMVNGAGLAMATMDIIKLKGGIPANFLDVGGSASEQAV
TEAFKILTKDPRVKCLLVNIFGGIXXCDIIASGIVNASKQVGLKIPLVVRLEGTNVNIGK
EILEKSGLNITSARDLDDAAXKN


Frame C:
---ktsinyssrvmqlksrlthslkppmvksfvwmlksismtmphsvkrrfsk*eippkr
ihvrlklvnlvsitlvsmvtlvvwlmvlvwlwppwisln*rvvsllissm*vvvplnkps
lkpsksslkiqelnvys*tfsvvsxnvislplvs*mlqnklvlkfh*lyv*kvqm*tlvk
kswknlv*illllei*mmllxk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFI894 (AFI894Q) /CSM/AF/AFI8-D/AFI894Q.Seq.d/                       1176   0.0
VFC521 (VFC521Q) /CSM/VF/VFC5-A/VFC521Q.Seq.d/                       1156   0.0
SSI167 (SSI167Q) /CSM/SS/SSI1-C/SSI167Q.Seq.d/                       1154   0.0
SFH344 (SFH344Q) /CSM/SF/SFH3-B/SFH344Q.Seq.d/                       1154   0.0
CFI546 (CFI546Q) /CSM/CF/CFI5-B/CFI546Q.Seq.d/                       1152   0.0
VFM558 (VFM558Q) /CSM/VF/VFM5-C/VFM558Q.Seq.d/                       1148   0.0
VFF749 (VFF749Q) /CSM/VF/VFF7-C/VFF749Q.Seq.d/                       1148   0.0
VFF668 (VFF668Q) /CSM/VF/VFF6-C/VFF668Q.Seq.d/                       1148   0.0
VFD829 (VFD829Q) /CSM/VF/VFD8-B/VFD829Q.Seq.d/                       1148   0.0
SFK777 (SFK777Q) /CSM/SF/SFK7-D/SFK777Q.Seq.d/                       1148   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.  1162   0.0    1
AC115594|AC115594.2 Dictyostelium discoideum chromosome 2 map 4071862-4101005 strain AX4, complete sequence.    52   3e-19  4
X84222|X84222.2 Neocallimastix frontalis mRNA for beta-succinyl CoA synthetase  precursor.    74   5e-18  4
AF493055|AF493055.1 Neocallimastix patriciarum succinyl-CoA synthetase, beta subunit (scsB) mRNA, complete cds.    74   6e-18  4
CO537257|CO537257.1 tah68f10.x1 Hydra EST UCI 5 Hydra magnipapillata cDNA 3' similar to TR:Q9YI36 Q9YI36 GTP-SPECIFIC SUCCINYL-COA SYNTHETASE BETA SUBUNIT ;, mRNA sequence.    70   3e-16  4
CV182872|CV182872.1 tai89g08.x1 Hydra EST UCI 5 ALP Hydra magnipapillata cDNA 3' similar to TR:Q9YI36 Q9YI36 GTP-SPECIFIC SUCCINYL-COA SYNTHETASE BETA SUBUNIT ;, mRNA sequence.    70   5e-16  4
AX621832|AX621832.1 Sequence 4795 from Patent WO02094868.          52   5e-16  4
CV151950|CV151950.1 taj38g08.x1 Hydra EST UCI 5 Hydra magnipapillata cDNA 3' similar to TR:Q9YI36 Q9YI36 GTP-SPECIFIC SUCCINYL-COA SYNTHETASE BETA SUBUNIT ;, mRNA sequence.    70   7e-16  4
AL034491|AL034491.1 S.pombe chromosome III cosmid c1620.           70   2e-14  2
AR354123|AR354123.1 Sequence 241 from patent US 6593114.           52   3e-14  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q6K9N6) RecName: Full=Succinyl-CoA ligase [GDP-forming] subunit...   293   3e-78
BT039713_1(BT039713|pid:none) Zea mays full-length cDNA clone ZM...   290   3e-77
EF530042_1(EF530042|pid:none) Caragana jubata succinyl-CoA ligas...   288   8e-77
AK221149_1(AK221149|pid:none) Arabidopsis thaliana mRNA for succ...   285   7e-76
(O82662) RecName: Full=Succinyl-CoA ligase [GDP-forming] subunit...   285   7e-76
AY087596_1(AY087596|pid:none) Arabidopsis thaliana clone 36904 m...   283   3e-75
DQ200398_1(DQ200398|pid:none) Solanum tuberosum clone 071C05 suc...   282   4e-75
DQ294279_1(DQ294279|pid:none) Solanum tuberosum clone 180E05 suc...   282   4e-75
CP000922_1730(CP000922|pid:none) Anoxybacillus flavithermus WK1,...   269   4e-71
BC084473_1(BC084473|pid:none) Xenopus tropicalis hypothetical LO...   269   5e-71

 psg: 0.75  gvh: 0.33  alm: 0.32  top: 0.53  tms: 0.00  mit: 0.16  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 28.0 %: nuclear
	 16.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: peroxisomal

>> prediction for AFI894 is cyt

>AFI894Z.Seq
----------TGGAAAACCTCTATCAATTATTCATCAAGAGTGATGCAACTCAAGTCGAG
ATTAACCCATTCGCTGAAACCACCGATGGTCAAGTCATTTGTATGGATGCTAAAATCAAT
TTCGATGACAATGCCTCATTCCGTCAAAAGGAGATTTTCGAAATGAGAGATACCGCCGAA
GAGGATCCACGTGAGGTTGAAGCTGGTAAATTTGGTCTCAATTACATTGGTCTCGATGGT
AACATTGGTTGTATGGTTAATGGTGCTGGTTTGGCTATGGCCACCATGGATATCATTAAA
TTAAAGGGTGGTATCCCTGCTAATTTCCTCGATGTAGGTGGTAGTGCCTCTGAACAAGCC
GTCACTGAAGCCTTCAAAATCCTCACTAAAGATCCAAGAGTTAAATGTTTACTCGTAAAC
ATTTTCGGTGGTATCATNNAATGTGATATCATTGCCTCTGGTATCGTAAATGCTTCAAAA
CAAGTTGGTCTTAAAATTCCATTAGTTGTACGTTTAGAAGGTACAAATGTAAACATTGGT
AAAGAAATCTTGGAAAAATCTGGTTTAAATATTACTTCTGCTAGAGATTTAGATGATGCT
GCTATNAAAAAC