>AFI889 (AFI889Q) /CSM/AF/AFI8-D/AFI889Q.Seq.d/
CATCAGCATCACCAAAATCAAGTAAAATATTAAATGTATCAGGATCAGGATCATCATCAT
CGTCATCATCATCATCGTCATCATCATCAAAAGATAAAGATAAAGATAAAAGAAAGAAAT
CATTTTTAAAAGATGATAGTGTACCATCGCTTTCATCAAAAACAGAGAATAGTTTATCAT
TAACATCATTATCAGAGATAACACAATCAATGACCAATACACAAACACAAACAGCAACAG
ATGCATCAATGACAGCAGAGGATGTATCAGGAGATTGGAGAAGATTATGATCCATTTTTA
TCAATACTTGAGGAACCAGGTGTCGATGGAGCAACCATTAAAAAAGAGACAACTGAAGAG
CTTCAAGAGATGTTGGCAGTATTAAAAGGTGTTCAAAATGAATGTAACGTTCTATTATTG
GGTAGAACTGGTGTTGGTAAATCATCGACATTGAATACGGTGTTTGGCATTGACATACCC
GTACATTCCTCAGAATCATGCACACAAGATCCATTCACCTATAGTAGAGTTGTGAATGGA
TTCAAATTGAATATCATTGATACACCAGGTTTTTTAGATTCAXXXXXXXXXXAAAACCAT
TCATTGTGTATTGTTTGTTGAGAAATTCACAGAGACAAGATTCGATGGTGCTCATCAATT
GGTGATCAATCAATTCACCGAGAAATTGGGTCCACAACTTTGGAGAAATACTGCCGTGGT
TTTAACTTATGCCAATAGTGTCTTGCCAGATTCTTGCTATGATGGTTTCGATGAGGAGGA
TGACGTCGGTCCTTGGAAAAAACATTATGAAGCAAGAGCTTTACAATTTAGAAAATTTTT
CGCTGGAATTTTGGCTCAATTGCCTCAAGATGATTATCCTCCAAAACATATACCAGTNTA
TGCTATGGAAAACTCTAGAAGATGTAAACGTAATGAACAAGGTCAAAGAGTACTCATTGA
TGGTACTCCTTGTCTTCATTTGTTAATTAGTGGTCTCTTGAAAATGGTTGACCCAAAAAC
TGCTTTCCTTTTTATGGGTCATCTTCGTGCCAAAAACAAACCTGGAAGAGGTCATCGTGG
AGATCAGAATGATAGAGAACNTTCAATCATGGATAATATCACTGAAATCCTTAAACTTTT
CATCGCTCCTCCTTTCGATCAATTGGGTAAAGGAACTGTCGCTAAAATATTGGAAAATTG
GGGTAAAAAATGGATAAAAAAG

hqhhqnqvky*myqdqdhhhrhhhhrhhhqkikikikernhf*kmivyhrfhqkqrivyh
*hhyqr*hnq*pihkhkqqqmhq*QQRMYQEIGEDYDPFLSILEEPGVDGATIKKETTEE
LQEMLAVLKGVQNECNVLLLGRTGVGKSSTLNTVFGIDIPVHSSESCTQDPFTYSRVVNG
FKLNIIDTPGFLDS---

---KTIHCVLFVEKFTETRFDGAHQLVINQFTEKLGPQLWRNTAVVLTYANSVLPDSCYD
GFDEEDDVGPWKKHYEARALQFRKFFAGILAQLPQDDYPPKHIPVYAMENSRRCKRNEQG
QRVLIDGTPCLHLLISGLLKMVDPKTAFLFMGHLRAKNKPGRGHRGDQNDREXSIMDNIT
EILKLFIAPPFDQLGKGTVAKILENWGKKWIKK

Frame A:
hqhhqnqvky*myqdqdhhhrhhhhrhhhqkikikikernhf*kmivyhrfhqkqrivyh
*hhyqr*hnq*pihkhkqqqmhq*QQRMYQEIGEDYDPFLSILEEPGVDGATIKKETTEE
LQEMLAVLKGVQNECNVLLLGRTGVGKSSTLNTVFGIDIPVHSSESCTQDPFTYSRVVNG
FKLNIIDTPGFLDS---

---KTIHCVLFVEKFTETRFDGAHQLVINQFTEKLGPQLWRNTAVVLTYANSVLPDSCYD
GFDEEDDVGPWKKHYEARALQFRKFFAGILAQLPQDDYPPKHIPVYAMENSRRCKRNEQG
QRVLIDGTPCLHLLISGLLKMVDPKTAFLFMGHLRAKNKPGRGHRGDQNDREXSIMDNIT
EILKLFIAPPFDQLGKGTVAKILENWGKKWIKK

Frame B:
isitkik*nikciririiiiviiiiviiikr*r*r*kkeiifkr**ctiafiknre*fii
niiirdntindqytntnsnrcindsrgcirrlekimihfyqylrnqvsmeqplkkrqlks
fkrcwqy*kvfkmnvtfyywvelvlvnhrh*irclaltypyipqnhahkihspivel*md
sn*islihqvf*i---

---kpfivycllrnsqrqdsmvlinw*sinsprnwvhnfgeilpwf*lmpivscqilamm
vsmrrmtsvlgknimkqelynlenfslefwlnclkmiilqniyqxmlwktledvnvmnkv
keyslmvllvfic*lvvs*kwltqkllsflwvifvpktnleeviveirmienxqswiisl
kslnfsslllsinwvkelslkywkigvkng*k

Frame C:
saspksskilnvsgsgssssssssssssskdkdkdkrkksflkddsvpslssktenslsl
tslseitqsmtntqtqtatdasmtaedvsgdwrrl*sifint*gtrcrwsnh*krdn*ra
srdvgsikrcsk*m*rsiig*nwcw*iidieygvwh*htrtflrimhtrsihl**scewi
qieyh*ytrffrf---

---nhslcivc*eihrdkirwcssigdqsihreigsttlekycrgfnlcq*clarfll*w
fr*gg*rrslektl*sksfti*kifrwnfgsiasr*lssktytsxcygkl*km*t**trs
ksth*wyslssfvn*wsleng*pkncfpfygssscqkqtwkrsswrse**rtfnhg*yh*
np*tfhrssfrsig*rncr*nigklg*kmdkk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFI889 (AFI889Q) /CSM/AF/AFI8-D/AFI889Q.Seq.d/                       2008   0.0
SSF840 (SSF840Q) /CSM/SS/SSF8-B/SSF840Q.Seq.d/                       1142   0.0
SFD114 (SFD114Q) /CSM/SF/SFD1-A/SFD114Q.Seq.d/                       1142   0.0
SSF732 (SSF732Q) /CSM/SS/SSF7-B/SSF732Q.Seq.d/                       1102   0.0
SSB394 (SSB394Q) /CSM/SS/SSB3-D/SSB394Q.Seq.d/                       1086   0.0
CFA840 (CFA840Q) /CSM/CF/CFA8-B/CFA840Q.Seq.d/                       1027   0.0
AFI343 (AFI343Q) /CSM/AF/AFI3-B/AFI343Q.Seq.d/                        991   0.0
SSK352 (SSK352Q) /CSM/SS/SSK3-C/SSK352Q.Seq.d/                        989   0.0
SSK821 (SSK821Q) /CSM/SS/SSK8-A/SSK821Q.Seq.d/                        888   0.0
AFN353 (AFN353Q) /CSM/AF/AFN3-C/AFN353Q.Seq.d/                        876   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC115598|AC115598.2 Dictyostelium discoideum chromosome 2 map 581427-735498 strain AX4, complete sequence.  1142   0.0    4
U83248|U83248.1 Dictyostelium discoideum GtpA (gtpA) mRNA, partial cds.   611   0.0    4
AC116982|AC116982.2 Dictyostelium discoideum chromosome 2 map 3622643-3879522 strain AX4, complete sequence.    32   0.018  17
BF695450|BF695450.1 601851842F1 NIH_MGC_56 Homo sapiens cDNA clone IMAGE:4075714 5', mRNA sequence.    42   0.21   2
AC126356|AC126356.2 Homo sapiens chromosome 17 clone CTD-2541I11 map 17, LOW-PASS SEQUENCE SAMPLING.    40   0.25   3
CR036655|CR036655.1 Forward strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP164a17.    46   1.3    1
CK255549|CK255549.1 EST739186 potato callus cDNA library, normalized and full-length Solanum tuberosum cDNA clone POCCM23 5' end, mRNA sequence.    46   1.3    1
CK272116|CK272116.1 EST718194 potato abiotic stress cDNA library Solanum tuberosum cDNA clone POAD325 5' end, mRNA sequence.    46   1.3    1
CR124918|CR124918.1 Reverse strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP164a17.    46   1.3    1
BG350487|BG350487.1 092D08 Mature tuber lambda ZAP Solanum tuberosum cDNA, mRNA sequence.    46   1.3    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC115598_34(AC115598|pid:none) Dictyostelium discoideum chromoso...   426   0.0
BT052621_1(BT052621|pid:none) Medicago truncatula clone MTYFD_FE...    62   2e-18
AM471105_1(AM471105|pid:none) Vitis vinifera contig VV78X226022....    64   3e-18
EU962452_1(EU962452|pid:none) Zea mays clone 242837 translocase ...    60   3e-17
AY496559_1(AY496559|pid:none) Physcomitrella patens chloroplast ...    58   5e-17
(Q41009) RecName: Full=Translocase of chloroplast 34;          E...    57   5e-17
DQ228327_1(DQ228327|pid:none) Solanum tuberosum clone 135F10 GTP...    60   1e-16
AJ132696_1(AJ132696|pid:none) Arabidopsis thaliana mRNA for chlo...    56   9e-16
CR954203_385(CR954203|pid:none) Ostreococcus tauri strain OTTH05...    53   6e-15
CP000583_389(CP000583|pid:none) Ostreococcus lucimarinus CCE9901...    54   1e-14

 psg: 0.75  gvh: 0.50  alm: 0.35  top: 0.53  tms: 0.00  mit: 0.26  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: nuclear
	 28.0 %: cytoplasmic
	 24.0 %: mitochondrial
	  8.0 %: vacuolar
	  4.0 %: peroxisomal
	  4.0 %: endoplasmic reticulum

>> prediction for AFI889 is nuc

>AFI889F.Seq
CATCAGCATCACCAAAATCAAGTAAAATATTAAATGTATCAGGATCAGGATCATCATCAT
CGTCATCATCATCATCGTCATCATCATCAAAAGATAAAGATAAAGATAAAAGAAAGAAAT
CATTTTTAAAAGATGATAGTGTACCATCGCTTTCATCAAAAACAGAGAATAGTTTATCAT
TAACATCATTATCAGAGATAACACAATCAATGACCAATACACAAACACAAACAGCAACAG
ATGCATCAATGACAGCAGAGGATGTATCAGGAGATTGGAGAAGATTATGATCCATTTTTA
TCAATACTTGAGGAACCAGGTGTCGATGGAGCAACCATTAAAAAAGAGACAACTGAAGAG
CTTCAAGAGATGTTGGCAGTATTAAAAGGTGTTCAAAATGAATGTAACGTTCTATTATTG
GGTAGAACTGGTGTTGGTAAATCATCGACATTGAATACGGTGTTTGGCATTGACATACCC
GTACATTCCTCAGAATCATGCACACAAGATCCATTCACCTATAGTAGAGTTGTGAATGGA
TTCAAATTGAATATCATTGATACACCAGGTTTTTTAGATTCA----------

>AFI889Z.Seq
----------AAAACCATTCATTGTGTATTGTTTGTTGAGAAATTCACAGAGACAAGATT
CGATGGTGCTCATCAATTGGTGATCAATCAATTCACCGAGAAATTGGGTCCACAACTTTG
GAGAAATACTGCCGTGGTTTTAACTTATGCCAATAGTGTCTTGCCAGATTCTTGCTATGA
TGGTTTCGATGAGGAGGATGACGTCGGTCCTTGGAAAAAACATTATGAAGCAAGAGCTTT
ACAATTTAGAAAATTTTTCGCTGGAATTTTGGCTCAATTGCCTCAAGATGATTATCCTCC
AAAACATATACCAGTNTATGCTATGGAAAACTCTAGAAGATGTAAACGTAATGAACAAGG
TCAAAGAGTACTCATTGATGGTACTCCTTGTCTTCATTTGTTAATTAGTGGTCTCTTGAA
AATGGTTGACCCAAAAACTGCTTTCCTTTTTATGGGTCATCTTCGTGCCAAAAACAAACC
TGGAAGAGGTCATCGTGGAGATCAGAATGATAGAGAACNTTCAATCATGGATAATATCAC
TGAAATCCTTAAACTTTTCATCGCTCCTCCTTTCGATCAATTGGGTAAAGGAACTGTCGC
TAAAATATTGGAAAATTGGGGTAAAAAATGGATAAAAAAG

>AFI889P.Seq
CATCAGCATCACCAAAATCAAGTAAAATATTAAATGTATCAGGATCAGGATCATCATCAT
CGTCATCATCATCATCGTCATCATCATCAAAAGATAAAGATAAAGATAAAAGAAAGAAAT
CATTTTTAAAAGATGATAGTGTACCATCGCTTTCATCAAAAACAGAGAATAGTTTATCAT
TAACATCATTATCAGAGATAACACAATCAATGACCAATACACAAACACAAACAGCAACAG
ATGCATCAATGACAGCAGAGGATGTATCAGGAGATTGGAGAAGATTATGATCCATTTTTA
TCAATACTTGAGGAACCAGGTGTCGATGGAGCAACCATTAAAAAAGAGACAACTGAAGAG
CTTCAAGAGATGTTGGCAGTATTAAAAGGTGTTCAAAATGAATGTAACGTTCTATTATTG
GGTAGAACTGGTGTTGGTAAATCATCGACATTGAATACGGTGTTTGGCATTGACATACCC
GTACATTCCTCAGAATCATGCACACAAGATCCATTCACCTATAGTAGAGTTGTGAATGGA
TTCAAATTGAATATCATTGATACACCAGGTTTTTTAGATTCA----------AAAACCAT
TCATTGTGTATTGTTTGTTGAGAAATTCACAGAGACAAGATTCGATGGTGCTCATCAATT
GGTGATCAATCAATTCACCGAGAAATTGGGTCCACAACTTTGGAGAAATACTGCCGTGGT
TTTAACTTATGCCAATAGTGTCTTGCCAGATTCTTGCTATGATGGTTTCGATGAGGAGGA
TGACGTCGGTCCTTGGAAAAAACATTATGAAGCAAGAGCTTTACAATTTAGAAAATTTTT
CGCTGGAATTTTGGCTCAATTGCCTCAAGATGATTATCCTCCAAAACATATACCAGTNTA
TGCTATGGAAAACTCTAGAAGATGTAAACGTAATGAACAAGGTCAAAGAGTACTCATTGA
TGGTACTCCTTGTCTTCATTTGTTAATTAGTGGTCTCTTGAAAATGGTTGACCCAAAAAC
TGCTTTCCTTTTTATGGGTCATCTTCGTGCCAAAAACAAACCTGGAAGAGGTCATCGTGG
AGATCAGAATGATAGAGAACNTTCAATCATGGATAATATCACTGAAATCCTTAAACTTTT
CATCGCTCCTCCTTTCGATCAATTGGGTAAAGGAACTGTCGCTAAAATATTGGAAAATTG
GGGTAAAAAATGGATAAAAAAG