>AFI881 (AFI881Q) /CSM/AF/AFI8-D/AFI881Q.Seq.d/
XXXXXXXXXXATTTATTCATTGACTCCACTCAAGATAACACTCAAGTTCTCGAAGATGTA
AAGAAAATCGCTACCTCACAAAAAGGTAATGCCGTTTTCTGTTGGGTCGATATGAAGAAA
TTCCCACAACAAGCAACTCATATGGGTCTCTCTGGTAAAGTTGTTCCAGCCATCTCTGTT
GATTCAGTTGCCAATAAAGCTCGTTACAATTTCGATGAAAAAGAAACCTTCTCATTCGAT
ACCGTCTCCAAATGGATCCAAGATGTCATTGGTGGTAAAGTTTCTCCATTTGTTAAATCT
CAACCAGAACCAGAATCAAATGATGCTCCAGTTAAGGTTGCCGTTGGTACCACTTTCAAA
AAGTTGGTTTTAGACTCACCAAAGGATGTTTTGGTTGAATTCTACGCTCCATGGTGCGGT
CATTGCAAGAATCTTGCTCCAATCTACGATAAATTAGGTGAATACTTAAAAGACGTTGAA
TCTGTCTCAATCGTAAAGATTGATGCCGACTCAAATGATGTACCAAGTGATATCGAAATT
AGAGGTTACCCAACCATCATGCTCTTCAAAGCTGATGACAAAGAAAATCCAATCTCTTAT
GAAGGTCAAAGAAATGATCATATGAACTTTGTTGAATTCATTCAAGATAACGCTGCCATT
GAATTCAAATTACCATCATCTCAAACTGATGATAATGTNAATCTAAAAAAGATTCTTCGC
TAAACANANAATTATAAA

---LFIDSTQDNTQVLEDVKKIATSQKGNAVFCWVDMKKFPQQATHMGLSGKVVPAISVD
SVANKARYNFDEKETFSFDTVSKWIQDVIGGKVSPFVKSQPEPESNDAPVKVAVGTTFKK
LVLDSPKDVLVEFYAPWCGHCKNLAPIYDKLGEYLKDVESVSIVKIDADSNDVPSDIEIR
GYPTIMLFKADDKENPISYEGQRNDHMNFVEFIQDNAAIEFKLPSSQTDDNVNLKKILR*
txnyk

Frame A:
---fih*lhsr*hsssrrckenryltkr*crfllgryeeipttsnsygslw*scsshlc*
fscq*sslqfr*krnlliryrlqmdprchww*sfsic*istrtrik*css*gcrwyhfqk
vgfrltkgcfg*ilrsmvrslqescsnlr*ir*ilkrr*iclnrkd*crlk*ctk*yrn*
rlpnhhalqs**qrksnll*rskk*syelc*ihsr*rch*iqitiisn***cxskkdssl
nxxl*


Frame B:
---LFIDSTQDNTQVLEDVKKIATSQKGNAVFCWVDMKKFPQQATHMGLSGKVVPAISVD
SVANKARYNFDEKETFSFDTVSKWIQDVIGGKVSPFVKSQPEPESNDAPVKVAVGTTFKK
LVLDSPKDVLVEFYAPWCGHCKNLAPIYDKLGEYLKDVESVSIVKIDADSNDVPSDIEIR
GYPTIMLFKADDKENPISYEGQRNDHMNFVEFIQDNAAIEFKLPSSQTDDNVNLKKILR*
txnyk


Frame C:
---ysltplkitlkfskm*rkslphkkvmpfsvgsi*rnshnkqliwvslvklfqpslli
qlpiklvtismkkkpshsipspngskmslvvkflhllnlnqnqnqmmlqlrlplvplsks
wf*thqrmfwlnstlhgaviarillqstin*vnt*ktlnlsqs*rlmptqmmyqviskle
vtqpscssklmtkkiqslmkvkemii*tllnsfkitlplnsnyhhlklmimxi*krffak
xxii

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFI881 (AFI881Q) /CSM/AF/AFI8-D/AFI881Q.Seq.d/                       1425   0.0
AFL563 (AFL563Q) /CSM/AF/AFL5-C/AFL563Q.Seq.d/                       1411   0.0
AFM464 (AFM464Q) /CSM/AF/AFM4-C/AFM464Q.Seq.d/                       1405   0.0
AFN679 (AFN679Q) /CSM/AF/AFN6-D/AFN679Q.Seq.d/                       1402   0.0
AFO479 (AFO479Q) /CSM/AF/AFO4-D/AFO479Q.Seq.d/                       1400   0.0
AFO368 (AFO368Q) /CSM/AF/AFO3-C/AFO368Q.Seq.d/                       1400   0.0
AFN855 (AFN855Q) /CSM/AF/AFN8-C/AFN855Q.Seq.d/                       1400   0.0
AFO686 (AFO686Q) /CSM/AF/AFO6-D/AFO686Q.Seq.d/                       1398   0.0
AFI432 (AFI432Q) /CSM/AF/AFI4-B/AFI432Q.Seq.d/                       1398   0.0
AFJ889 (AFJ889Q) /CSM/AF/AFJ8-D/AFJ889Q.Seq.d/                       1396   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BJ171852|BJ171852.1 Physcomitrella patens subsp. patens cDNA clone:pph30f12, 3' end,single read.   599   e-167  1
CN206669|CN206669.1 Tor7097 Gametophyte rehydration Library Tortula ruralis cDNA, mRNA sequence.    78   2e-10  1
CN591465|CN591465.1 TTE00010235 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   4e-09  1
CN597971|CN597971.1 TTE00009997 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   4e-09  1
CN590755|CN590755.1 TTE00006978 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   4e-09  1
BM398179|BM398179.1 5009-0-41-G11.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    74   4e-09  1
CN206834|CN206834.1 Tor7258 Gametophyte rehydration Library Tortula ruralis cDNA, mRNA sequence.    70   6e-08  1
CK030146|CK030146.1 AGENCOURT_16621384 NIH_ZGC_7 Danio rerio cDNA clone IMAGE:7052631 5', mRNA sequence.    70   6e-08  1
AL924030|AL924030.1 Danio rerio EST sequence, clone 134-B07-2.     70   6e-08  1
BC067155|BC067155.1 Danio rerio zgc:77086, mRNA (cDNA clone MGC:77086 IMAGE:6960343), complete cds.    70   6e-08  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q12730) RecName: Full=Protein disulfide-isomerase;          Sho...   177   3e-43
(Q00248) RecName: Full=Protein disulfide-isomerase;          Sho...   174   2e-42
EU016183_1(EU016183|pid:none) Haemaphysalis longicornis strain G...   173   5e-42
CU633901_146(CU633901|pid:none) Podospora anserina genomic DNA c...   168   1e-40
DQ066336_1(DQ066336|pid:none) Ixodes scapularis isolate IS-6-12-...   168   2e-40
DQ377176_1(DQ377176|pid:none) Amblyomma variegatum protein disul...   163   6e-39
AE013599_1413(AE013599|pid:none) Drosophila melanogaster chromos...   154   2e-36
CR382131_125(CR382131|pid:none) Yarrowia lipolytica strain CLIB1...   152   8e-36
(Q10057) RecName: Full=Putative protein disulfide-isomerase C1F5...   152   1e-35
AB022013_1(AB022013|pid:none) Antheraea pernyi mRNA for protein ...   151   2e-35

 psg: 0.75  gvh: 0.36  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.27  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 36.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: vacuolar
	  4.0 %: endoplasmic reticulum
	  4.0 %: peroxisomal

>> prediction for AFI881 is cyt

>AFI881Z.Seq
----------ATTTATTCATTGACTCCACTCAAGATAACACTCAAGTTCTCGAAGATGTA
AAGAAAATCGCTACCTCACAAAAAGGTAATGCCGTTTTCTGTTGGGTCGATATGAAGAAA
TTCCCACAACAAGCAACTCATATGGGTCTCTCTGGTAAAGTTGTTCCAGCCATCTCTGTT
GATTCAGTTGCCAATAAAGCTCGTTACAATTTCGATGAAAAAGAAACCTTCTCATTCGAT
ACCGTCTCCAAATGGATCCAAGATGTCATTGGTGGTAAAGTTTCTCCATTTGTTAAATCT
CAACCAGAACCAGAATCAAATGATGCTCCAGTTAAGGTTGCCGTTGGTACCACTTTCAAA
AAGTTGGTTTTAGACTCACCAAAGGATGTTTTGGTTGAATTCTACGCTCCATGGTGCGGT
CATTGCAAGAATCTTGCTCCAATCTACGATAAATTAGGTGAATACTTAAAAGACGTTGAA
TCTGTCTCAATCGTAAAGATTGATGCCGACTCAAATGATGTACCAAGTGATATCGAAATT
AGAGGTTACCCAACCATCATGCTCTTCAAAGCTGATGACAAAGAAAATCCAATCTCTTAT
GAAGGTCAAAGAAATGATCATATGAACTTTGTTGAATTCATTCAAGATAACGCTGCCATT
GAATTCAAATTACCATCATCTCAAACTGATGATAATGTNAATCTAAAAAAGATTCTTCGC
TAAACANANAATTATAAA