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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFI865
Library AF
(Link to library)
Clone ID AFI865
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15345-1
Original site URL
Representative seq. ID AFI865P
(Link to Original site)
Representative DNA sequence 
>AFI865 (AFI865Q) /CSM/AF/AFI8-C/AFI865Q.Seq.d/
CAACCATTATTGGTTATTTTAAAAGAGGCAAAACCTTTACAGCAAATTTAATAAATAATA
TTAATTTACCATCTGGTAGTAAAACTAAAACAAAAGGTTTATCAATTTATAATTCTCATA
CAAATGATACTTTATTTATTGATACTCAAGGTAGTAATACTCCTCTACCATTTTTTGTAG
ATGAAAATGAAAATGAAGAACTTAAAAAAGCTTATAATATCAATCAAGCTGATGGAAGTA
TTAAATTGAATAATCTTCATCTTGCACGTAAGAAAGCAACTGAATCATTCATTCAAGAGA
CTGCTATTAGTTTATCAAATGTTGTAATTTTCGTTGTTAATGAAATGACATGGCACGATC
AACAAAATATAATGGCCATTCAAAAGAAGATTAAAACTCAAAATCCAATTGAAAAACAAA
AAATCAGAGAACTCTATGTGTTTCATAATTATATGAATATTTCAAATATGGTTGATCTAA
TGGCAATGATTAAAACCCTATGTAACAACTCCATTCAAAGGTTATTTTCATACTGAATTC
AGCCTTCATTCTGATAATGAATATATAATTTATTATACAGATGCCGAGAATTTTACAACT
CATTTCTXXXXXXXXXXAACAGATTTAAAAATTGTACCATATTATAATTCTCAAACTCAA
GTTGTGGAAATTGAAAATAAATCATCGAGTACAAGATCAAACACATTTGTATATAAGAGA
CAGACTGGTATACCATTAAAGGATCAAAAAACATTGGAAGATTTAGAAGAAATGTTCAAT
CAAGTTAAAGCACATACTATTGATATTAACCAAGTTCCAATGTATTCGATTGTACCTGCT
ATTAAAAATAATGAATATGAGCAATGGAAATATGCATTAAATAACAAGGATATCATTTTC
ATTGATAATCTATTGATTGTAACTGGAAGTTTCAATCCAACCTATGACAGTCTTGAATGT
GAGAATCGTACATTAATATTCACATTTGATTTGCCAGATATTCCAGAATCTCATTTCTCT
CATTCAATCTACTATGATCCAAGTGACAAAGTTAGATACTTAAAAGTCTCGGGTAATAGA
AAATTAAGTTATAGAGCATTAACTGGTACACGTGGTGGTGAGAGTACAATTAATGAATAT
CCATCAGCTGGTGTTAAAATTGGTAATAGTTTAAATCTTAGAAAAGATGGAGAATTTGAT
CTGAGAATTCCAATTGAAGCAAATTATAGTAAAAATTATTCAAATCATTTTGAAAATGGT
GTTTTAACATTAACATTTAAAGAAGAAATNAAGGTCTCCAGA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
qpllvilkeakplqqi**iiliyhlvvklkqkvyqfiiliqmilyllilkvvillyhfl*
mkmkmknlkkliisiklmevln*iifilhvrkqlnhsfkrlllvyqml*fsllmk*hgti
nki*wpfkrrlklkiqlknkksensmcfiii*ifqiwli*wq*LKPYVTTPFKGYFHTEF
SLHSDNEYIIYYTDAENFTTHF---

---TDLKIVPYYNSQTQVVEIENKSSSTRSNTFVYKRQTGIPLKDQKTLEDLEEMFNQVK
AHTIDINQVPMYSIVPAIKNNEYEQWKYALNNKDIIFIDNLLIVTGSFNPTYDSLECENR
TLIFTFDLPDIPESHFSHSIYYDPSDKVRYLKVSGNRKLSYRALTGTRGGESTINEYPSA
GVKIGNSLNLRKDGEFDLRIPIEANYSKNYSNHFENGVLTLTFKEEXKVSR
Translated Amino Acid sequence (All Frames) 
Frame A:
qpllvilkeakplqqi**iiliyhlvvklkqkvyqfiiliqmilyllilkvvillyhfl*
mkmkmknlkkliisiklmevln*iifilhvrkqlnhsfkrlllvyqml*fsllmk*hgti
nki*wpfkrrlklkiqlknkksensmcfiii*ifqiwli*wq*LKPYVTTPFKGYFHTEF
SLHSDNEYIIYYTDAENFTTHF---

---nrfknctil*fsnsscgn*k*iieykikhici*etdwytikgsknigrfrrnvqss*
styy*y*pssnvfdctcy*k**i*ameicik*qgyhfh**sidcnwkfqsnl*qs*m*es
yinihi*farysrisflsfnll*sk*qs*ilkslg**kikl*sinwytww*eyn**isis
wc*nw**fks*krwri*sensn*skl**klfksf*kwcfnini*rrnxglq

Frame B:
nhywlf*krqnlyskfnk*y*ftiw**n*nkrfinl*fsyk*yfiy*ysr**ysstifcr
*k*k*rt*ksl*yqss*wky*ie*sssct*esn*iihsrdcy*fikccnfrc**ndmars
tkynghsked*nsksn*ktknqrtlcvs*lyeyfkyg*sngnd*npm*qlhskvifilns
afilimni*fiiqmprilqlis---

---TDLKIVPYYNSQTQVVEIENKSSSTRSNTFVYKRQTGIPLKDQKTLEDLEEMFNQVK
AHTIDINQVPMYSIVPAIKNNEYEQWKYALNNKDIIFIDNLLIVTGSFNPTYDSLECENR
TLIFTFDLPDIPESHFSHSIYYDPSDKVRYLKVSGNRKLSYRALTGTRGGESTINEYPSA
GVKIGNSLNLRKDGEFDLRIPIEANYSKNYSNHFENGVLTLTFKEEXKVSR

Frame C:
tiigyfkrgktftanlinninlpsgsktktkglsiynshtndtlfidtqgsntplpffvd
eneneelkkayninqadgsiklnnlhlarkkatesfiqetaislsnvvifvvnemtwhdq
qnimaiqkkiktqnpiekqkirelyvfhnymnisnmvdlmamiktlcnnsiqrlfsy*iq
psf***iynllyrcrefynsf---

---qi*klyhiiilklklwklkinhrvqdqthlyirdrlvyh*rikkhwki*kkcsiklk
hilliltkfqcirlylllkimnmsngnmh*itrisfsliiy*l*levsiqpmtvlnvriv
h*yshlicqifqnlisliqstmiqvtkldt*ksrvien*vieh*lvhvvvrvqlmnihql
vlklviv*ilekmenli*efqlkqiivkiiqiilkmvf*h*hlkkkxrsp
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFI865 (AFI865Q) /CSM/AF/AFI8-C/AFI865Q.Seq.d/                       2444   0.0
AHD861 (AHD861Q) /CSM/AH/AHD8-C/AHD861Q.Seq.d/                       1324   0.0
AHI704 (AHI704Q) /CSM/AH/AHI7-A/AHI704Q.Seq.d/                       1287   0.0
AHE564 (AHE564Q) /CSM/AH/AHE5-C/AHE564Q.Seq.d/                       1287   0.0
AHL245 (AHL245Q) /CSM/AH/AHL2-B/AHL245Q.Seq.d/                       1249   0.0
AFK186 (AFK186Q) /CSM/AF/AFK1-D/AFK186Q.Seq.d/                       1207   0.0
CHH490 (CHH490Q) /CSM/CH/CHH4-D/CHH490Q.Seq.d/                       1193   0.0
CHI178 (CHI178Q) /CSM/CH/CHI1-D/CHI178Q.Seq.d/                       1191   0.0
AFA349 (AFA349Q) /CSM/AF/AFA3-C/AFA349Q.Seq.d/                       1181   0.0
AHL469 (AHL469Q) /CSM/AH/AHL4-C/AHL469Q.Seq.d/                       1174   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U82514|U82514.1 Dictyostelium discoideum random slug cDNA6 protein (rsc6) mRNA, partial cds.   908   0.0    1
AE017263|AE017263.1 Mesoplasma florum L1 complete genome.          36   4e-10  31
AL929356|AL929356.1 Plasmodium falciparum strain 3D7, chromosome 9; segment 2/5.    36   1e-08  21
AC116963|AC116963.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.    34   2e-08  21
CR382399|CR382399.1 Plasmodium falciparum chromosome 6, complete sequence; segment 2/5.    36   2e-08  21
AX344558|AX344558.1 Sequence 9 from Patent WO0200932.              34   2e-07  16
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    38   2e-07  19
AP004170|AP004170.1 Mycoplasma penetrans genomic DNA, complete sequence, section 1/5.    36   2e-07  19
AL844509|AL844509.1 Plasmodium falciparum chromosome 13.           40   3e-07  25
AX344559|AX344559.1 Sequence 10 from Patent WO0200932.             38   1e-06  20
dna update 2004. 9.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U82514_1(U82514|pid:none) Dictyostelium discoideum random slug c...   315   2e-84
(Q54QE8) RecName: Full=Heat shock protein DDB_G0283913;               112   4e-27
AL844507_236(AL844507|pid:none) Plasmodium falciparum 3D7 chromo...    44   0.012
CP001034_1189(CP001034|pid:none) Natranaerobius thermophilus JW/...    42   0.035
BA000026_949(BA000026|pid:none) Mycoplasma penetrans HF-2 DNA, c...    41   0.078
AY166677_1(AY166677|pid:none) Plasmodium reichenowi reticulocyte...    41   0.078
CP001014_1329(CP001014|pid:none) Thermoproteus neutrophilus V24S...    39   0.39
AE001362_113(AE001362|pid:none) Plasmodium falciparum 3D7 chromo...    38   0.86
CP001185_1710(CP001185|pid:none) Thermosipho africanus TCF52B, c...    37   1.1
AC116979_66(AC116979|pid:none) Dictyostelium discoideum chromoso...    37   1.1
protein update 2009. 4.23
PSORT 

 psg: 0.74  gvh: 0.20  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.28  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 32.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: plasma membrane
	  4.0 %: extracellular, including cell wall

>> prediction for AFI865 is nuc
5' end seq. ID AFI865F
5' end seq. 
>AFI865F.Seq
CAACCATTATTGGTTATTTTAAAAGAGGCAAAACCTTTACAGCAAATTTAATAAATAATA
TTAATTTACCATCTGGTAGTAAAACTAAAACAAAAGGTTTATCAATTTATAATTCTCATA
CAAATGATACTTTATTTATTGATACTCAAGGTAGTAATACTCCTCTACCATTTTTTGTAG
ATGAAAATGAAAATGAAGAACTTAAAAAAGCTTATAATATCAATCAAGCTGATGGAAGTA
TTAAATTGAATAATCTTCATCTTGCACGTAAGAAAGCAACTGAATCATTCATTCAAGAGA
CTGCTATTAGTTTATCAAATGTTGTAATTTTCGTTGTTAATGAAATGACATGGCACGATC
AACAAAATATAATGGCCATTCAAAAGAAGATTAAAACTCAAAATCCAATTGAAAAACAAA
AAATCAGAGAACTCTATGTGTTTCATAATTATATGAATATTTCAAATATGGTTGATCTAA
TGGCAATGATTAAAACCCTATGTAACAACTCCATTCAAAGGTTATTTTCATACTGAATTC
AGCCTTCATTCTGATAATGAATATATAATTTATTATACAGATGCCGAGAATTTTACAACT
CATTTCT----------
Length of 5' end seq. 607
3' end seq. ID AFI865Z
3' end seq. 
>AFI865Z.Seq
----------AACAGATTTAAAAATTGTACCATATTATAATTCTCAAACTCAAGTTGTGG
AAATTGAAAATAAATCATCGAGTACAAGATCAAACACATTTGTATATAAGAGACAGACTG
GTATACCATTAAAGGATCAAAAAACATTGGAAGATTTAGAAGAAATGTTCAATCAAGTTA
AAGCACATACTATTGATATTAACCAAGTTCCAATGTATTCGATTGTACCTGCTATTAAAA
ATAATGAATATGAGCAATGGAAATATGCATTAAATAACAAGGATATCATTTTCATTGATA
ATCTATTGATTGTAACTGGAAGTTTCAATCCAACCTATGACAGTCTTGAATGTGAGAATC
GTACATTAATATTCACATTTGATTTGCCAGATATTCCAGAATCTCATTTCTCTCATTCAA
TCTACTATGATCCAAGTGACAAAGTTAGATACTTAAAAGTCTCGGGTAATAGAAAATTAA
GTTATAGAGCATTAACTGGTACACGTGGTGGTGAGAGTACAATTAATGAATATCCATCAG
CTGGTGTTAAAATTGGTAATAGTTTAAATCTTAGAAAAGATGGAGAATTTGATCTGAGAA
TTCCAATTGAAGCAAATTATAGTAAAAATTATTCAAATCATTTTGAAAATGGTGTTTTAA
CATTAACATTTAAAGAAGAAATNAAGGTCTCCAGA
Length of 3' end seq. 685
Connected seq. ID AFI865P
Connected seq. 
>AFI865P.Seq
CAACCATTATTGGTTATTTTAAAAGAGGCAAAACCTTTACAGCAAATTTAATAAATAATA
TTAATTTACCATCTGGTAGTAAAACTAAAACAAAAGGTTTATCAATTTATAATTCTCATA
CAAATGATACTTTATTTATTGATACTCAAGGTAGTAATACTCCTCTACCATTTTTTGTAG
ATGAAAATGAAAATGAAGAACTTAAAAAAGCTTATAATATCAATCAAGCTGATGGAAGTA
TTAAATTGAATAATCTTCATCTTGCACGTAAGAAAGCAACTGAATCATTCATTCAAGAGA
CTGCTATTAGTTTATCAAATGTTGTAATTTTCGTTGTTAATGAAATGACATGGCACGATC
AACAAAATATAATGGCCATTCAAAAGAAGATTAAAACTCAAAATCCAATTGAAAAACAAA
AAATCAGAGAACTCTATGTGTTTCATAATTATATGAATATTTCAAATATGGTTGATCTAA
TGGCAATGATTAAAACCCTATGTAACAACTCCATTCAAAGGTTATTTTCATACTGAATTC
AGCCTTCATTCTGATAATGAATATATAATTTATTATACAGATGCCGAGAATTTTACAACT
CATTTCT----------AACAGATTTAAAAATTGTACCATATTATAATTCTCAAACTCAA
GTTGTGGAAATTGAAAATAAATCATCGAGTACAAGATCAAACACATTTGTATATAAGAGA
CAGACTGGTATACCATTAAAGGATCAAAAAACATTGGAAGATTTAGAAGAAATGTTCAAT
CAAGTTAAAGCACATACTATTGATATTAACCAAGTTCCAATGTATTCGATTGTACCTGCT
ATTAAAAATAATGAATATGAGCAATGGAAATATGCATTAAATAACAAGGATATCATTTTC
ATTGATAATCTATTGATTGTAACTGGAAGTTTCAATCCAACCTATGACAGTCTTGAATGT
GAGAATCGTACATTAATATTCACATTTGATTTGCCAGATATTCCAGAATCTCATTTCTCT
CATTCAATCTACTATGATCCAAGTGACAAAGTTAGATACTTAAAAGTCTCGGGTAATAGA
AAATTAAGTTATAGAGCATTAACTGGTACACGTGGTGGTGAGAGTACAATTAATGAATAT
CCATCAGCTGGTGTTAAAATTGGTAATAGTTTAAATCTTAGAAAAGATGGAGAATTTGAT
CTGAGAATTCCAATTGAAGCAAATTATAGTAAAAATTATTCAAATCATTTTGAAAATGGT
GTTTTAACATTAACATTTAAAGAAGAAATNAAGGTCTCCAGA
Length of connected seq. 1292
Full length Seq ID -
Full length Seq. -
Length of full length seq. -