>SHC223 (SHC223Q) /CSM/SH/SHC2-A/SHC223Q.Seq.d/
AAAATTTTAATTATTGTATATAATATTTTTAAATATATATATTAATATATATTAATATAT
TAATATTAATAAAATGAAAATATTATATTCATTATTATTAATATCAAGTATAATTTTAAA
TACTGTATTAAATATTTCATCACAAGTATATGATCAACGTATTTTGGCATTAAAAGGAAA
ATGTTATTTAGATTCAAATAAAAATGCATTGTATGATGAAGGTGAACCAGGATTTGGAAA
TGTTGAAATTTCTTTAGGAAATGCAACTGATTATAAGGGAAATAAATATCCAATACGTTA
TTCATCTGCTGATGGTACATTTATATATGATGGATTAACTCAACGTACAACTTATGAATT
TACATTTACCAATCCAAAAGATTATTACTTTGAAACCTATGAAAAGACATTGGCTTGTCC
ACCAGGTGTACCATGTATTCGTCCAACGGTTTCAGCTGATAATTTGGCAGCAGATCTTTT
AGTTGCAAATACCTTTGCCTATAACATTCCATTGATTAAACAAGTTGGTGTTTCATCAGG
CTCATCTATACTTACAGTTAGAATTAGTGTTGATTTGGGTTATGTTGAGCAAACTCAACC
ACCAACTCAACCACCTACTCAACCACCTACTCAACCACCAACACCTCCAXXXXXXXXXXG
AGGTAAATGTGGTAGTCTTGTTGGTGCCGGAGTTGATCAAAAATCACTCACTGGTCTTAG
ATATGGTCCAGGTAATGGTAATTTCTTTGATAATCATAAAACAGGTCTTCATGACTATAC
CAATGCTTTTGGTGCAACTAAAGGTGGTCGTGATATTATTGTTACAACTGGTTTCGATTA
CTATGAAGCATTTGAAGGTTCAATTCGTTGGTATAATGCAACCTCTGGTAAACAACTAAA
AGGTTACTCTTTATATATAACCTCTTCAAATGGTCGTACCCCATCTCCAACCTTTGGTAA
ACAAAATGGTCTTGGTGATATTACTTCAGTGTATGATTCTTCTCTTCCACAATATCGTGT
TGGTAGAGTTTGGCTTGATATAAATGGTAATGGTATTCAAGATGCTGGTGAACCTGGTAT
TTCAGGAATCACAGTATTCTTAATCTATGGTCAACAATCAACTCCAACAAGTCAAACTGT
ATCCGATTCAAATGGTTATTTCAAATTTGAAGTTGATTTCAGTTCAAATTATTATTGTGT
AATTCCTGTTGCATTCTTCCAAGCTGGTTCTTCCATTACAATGAGAAATGCTGATCCAAC
TCATCCAAATACAAATAGTGATGCTTTCTTATCAACTGTTGATAATGTTTATGTCACTTC
TTTTACTTCTTCTGATGCTGGGGTTATTATTTTAATGGTTGTTCATTGGTATTATTCCAA
AAAAATAAAGTAATAAAAAAATA

kf*llyiiflniyiniy*YININKMKILYSLLLISSIILNTVLNISSQVYDQRILALKGK
CYLDSNKNALYDEGEPGFGNVEISLGNATDYKGNKYPIRYSSADGTFIYDGLTQRTTYEF
TFTNPKDYYFETYEKTLACPPGVPCIRPTVSADNLAADLLVANTFAYNIPLIKQVGVSSG
SSILTVRISVDLGYVEQTQPPTQPPTQPPTQPPTPP---

---GKCGSLVGAGVDQKSLTGLRYGPGNGNFFDNHKTGLHDYTNAFGATKGGRDIIVTTG
FDYYEAFEGSIRWYNATSGKQLKGYSLYITSSNGRTPSPTFGKQNGLGDITSVYDSSLPQ
YRVGRVWLDINGNGIQDAGEPGISGITVFLIYGQQSTPTSQTVSDSNGYFKFEVDFSSNY
YCVIPVAFFQAGSSITMRNADPTHPNTNSDAFLSTVDNVYVTSFTSSDAGVIILMVVHWY
YSKKIK**kn

Frame A:
kiliivynifkyiy*yiliy*y**neniifiiinikynfkycikyfitsi*styfgikrk
mlfrfk*kciv**r*triwkc*nffrkcn*l*gk*isntlfic*wyiyi*winstynl*i
yiyqskrlll*nl*kdiglstrctmyssngfs**fgsrsfsckylcl*hsid*tswcfir
liytys*n*c*fglc*ansttnsttysttysttnts---

---evnvvvllvpeliknhslvldmvqvmvisliiikqvfmtipmllvqlkvvvilllql
vsitmkhlkvqfvgimqplvnn*kvtlyi*plqmvvphlqplvnkmvlvillqcmillfh
nivlvefgli*mvmvfkmlvnlvfqesqys*smvnnqlqqvklypiqmvisnlklisvqi
iiv*fllhssklvlplq*emliqliqiqivmlsyqllimfmsllllllmlgllf*wlfig
iipkk*snkki

Frame B:
kf*llyiiflniyiniy*YININKMKILYSLLLISSIILNTVLNISSQVYDQRILALKGK
CYLDSNKNALYDEGEPGFGNVEISLGNATDYKGNKYPIRYSSADGTFIYDGLTQRTTYEF
TFTNPKDYYFETYEKTLACPPGVPCIRPTVSADNLAADLLVANTFAYNIPLIKQVGVSSG
SSILTVRISVDLGYVEQTQPPTQPPTQPPTQPPTPP---

---r*mw*scwcrs*skithws*iwsr*w*fl**s*nrss*lyqcfwcn*rws*yycynw
frll*si*rfnslv*cnlw*ttkrllfiynlfkwsypisnlw*tkwsw*yyfsv*ffsst
iscw*sla*ykw*wysrcw*twyfrnhsilnlwstinsnksncirfkwlfqi*s*fqfkl
llcnsccilpswffhynekc*snsskyk**cflinc**clchffyff*cwgyyfngcslv
lfqknkvikk

Frame C:
nfnyci*yf*iyiliyinililik*kyyihyy*yqv*f*ily*ifhhkyminvfwh*ken
vi*iqikmhcmmkvnqdlemlkfl*emqliireiniqyvihllmvhlymmd*lnvqlmnl
hlpiqkiitlkpmkrhwlvhqvyhvfvqrfqliiwqqif*lqiplpitfh*lnklvfhqa
hlylqlelvliwvmlsklnhqlnhllnhllnhqhl---

---GKCGSLVGAGVDQKSLTGLRYGPGNGNFFDNHKTGLHDYTNAFGATKGGRDIIVTTG
FDYYEAFEGSIRWYNATSGKQLKGYSLYITSSNGRTPSPTFGKQNGLGDITSVYDSSLPQ
YRVGRVWLDINGNGIQDAGEPGISGITVFLIYGQQSTPTSQTVSDSNGYFKFEVDFSSNY
YCVIPVAFFQAGSSITMRNADPTHPNTNSDAFLSTVDNVYVTSFTSSDAGVIILMVVHWY
YSKKIK**kn

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHC223 (SHC223Q) /CSM/SH/SHC2-A/SHC223Q.Seq.d/                       2498   0.0
SHC160 (SHC160Q) /CSM/SH/SHC1-C/SHC160Q.Seq.d/                       2486   0.0
SHL582 (SHL582Q) /CSM/SH/SHL5-D/SHL582Q.Seq.d/                       1425   0.0
SHL103 (SHL103Q) /CSM/SH/SHL1-A/SHL103Q.Seq.d/                       1425   0.0
SHG836 (SHG836Q) /CSM/SH/SHG8-B/SHG836Q.Seq.d/                       1425   0.0
SHD494 (SHD494Q) /CSM/SH/SHD4-D/SHD494Q.Seq.d/                       1425   0.0
SHD279 (SHD279Q) /CSM/SH/SHD2-D/SHD279Q.Seq.d/                       1425   0.0
SHA857 (SHA857Q) /CSM/SH/SHA8-C/SHA857Q.Seq.d/                       1425   0.0
SHA769 (SHA769Q) /CSM/SH/SHA7-C/SHA769Q.Seq.d/                       1425   0.0
SHA116 (SHA116Q) /CSM/SH/SHA1-A/SHA116Q.Seq.d/                       1425   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF066073|AF066073.1 Dictyostelium discoideum SP85 (pspB) gene, partial cds.    62   3e-05  1
AF066071|AF066071.1 Dictyostelium discoideum SP85 (pspB) gene, complete cds.    62   3e-05  1
AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.    62   3e-05  1
CK808839|CK808839.1 Rasgsc1686 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara americana cDNA, mRNA sequence.    34   4e-05  3
CK808739|CK808739.1 Rasgsc1529 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara americana cDNA, mRNA sequence.    32   5e-04  3
S72639|S72639.1 PspB=prespore-specific gene {5' region} [Dictyostelium discoideum, Genomic, 1918 nt].    58   5e-04  1
AF066072|AF066072.1 Dictyostelium discoideum SP85 (pspB) gene, partial cds.    58   5e-04  1
AC116963|AC116963.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.    58   5e-04  1
CK808037|CK808037.1 Rasgsc0316 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara americana cDNA, mRNA sequence.    34   0.002  3
DT775356|DT775356.1 124719991 TH1 Tribolium castaneum cDNA clone 3C7 3', mRNA sequence.    54   0.008  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP000686_3542(CP000686|pid:none) Roseiflexus sp. RS-1, complete ...    78   8e-13
CP000909_404(CP000909|pid:none) Chloroflexus aurantiacus J-10-fl...    63   2e-08
CP000910_1528(CP000910|pid:none) Renibacterium salmoninarum ATCC...    61   1e-07
CP001337_3037(CP001337|pid:none) Chloroflexus aggregans DSM 9485...    59   4e-07
(Q7A780) RecName: Full=Serine-aspartate repeat-containing protei...    53   3e-05
AM076203_1(AM076203|pid:none) Staphylococcus aureus partial sdrD...    53   3e-05
AM076200_1(AM076200|pid:none) Staphylococcus aureus partial sdrD...    53   3e-05
AM076196_1(AM076196|pid:none) Staphylococcus aureus partial sdrD...    53   3e-05
AM076198_1(AM076198|pid:none) Staphylococcus aureus partial sdrD...    53   3e-05
CP000703_575(CP000703|pid:none) Staphylococcus aureus subsp. aur...    53   3e-05

 psg: 0.95  gvh: 0.59  alm: 0.26  top: 0.40  tms: 0.07  mit: 0.31  mip: 0.07
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.56  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: mitochondrial
	 20.0 %: cytoplasmic
	 20.0 %: Golgi
	 12.0 %: nuclear
	 12.0 %: endoplasmic reticulum
	  4.0 %: extracellular, including cell wall
	  4.0 %: plasma membrane

>> prediction for SHC223 is mit

>SHC223F.Seq
AAAATTTTAATTATTGTATATAATATTTTTAAATATATATATTAATATATATTAATATAT
TAATATTAATAAAATGAAAATATTATATTCATTATTATTAATATCAAGTATAATTTTAAA
TACTGTATTAAATATTTCATCACAAGTATATGATCAACGTATTTTGGCATTAAAAGGAAA
ATGTTATTTAGATTCAAATAAAAATGCATTGTATGATGAAGGTGAACCAGGATTTGGAAA
TGTTGAAATTTCTTTAGGAAATGCAACTGATTATAAGGGAAATAAATATCCAATACGTTA
TTCATCTGCTGATGGTACATTTATATATGATGGATTAACTCAACGTACAACTTATGAATT
TACATTTACCAATCCAAAAGATTATTACTTTGAAACCTATGAAAAGACATTGGCTTGTCC
ACCAGGTGTACCATGTATTCGTCCAACGGTTTCAGCTGATAATTTGGCAGCAGATCTTTT
AGTTGCAAATACCTTTGCCTATAACATTCCATTGATTAAACAAGTTGGTGTTTCATCAGG
CTCATCTATACTTACAGTTAGAATTAGTGTTGATTTGGGTTATGTTGAGCAAACTCAACC
ACCAACTCAACCACCTACTCAACCACCTACTCAACCACCAACACCTCCANNNNNNNNNN

>SHC223Z.Seq
NNNNNNNNNNGAGGTAAATGTGGTAGTCTTGTTGGTGCCGGAGTTGATCAAAAATCACTC
ACTGGTCTTAGATATGGTCCAGGTAATGGTAATTTCTTTGATAATCATAAAACAGGTCTT
CATGACTATACCAATGCTTTTGGTGCAACTAAAGGTGGTCGTGATATTATTGTTACAACT
GGTTTCGATTACTATGAAGCATTTGAAGGTTCAATTCGTTGGTATAATGCAACCTCTGGT
AAACAACTAAAAGGTTACTCTTTATATATAACCTCTTCAAATGGTCGTACCCCATCTCCA
ACCTTTGGTAAACAAAATGGTCTTGGTGATATTACTTCAGTGTATGATTCTTCTCTTCCA
CAATATCGTGTTGGTAGAGTTTGGCTTGATATAAATGGTAATGGTATTCAAGATGCTGGT
GAACCTGGTATTTCAGGAATCACAGTATTCTTAATCTATGGTCAACAATCAACTCCAACA
AGTCAAACTGTATCCGATTCAAATGGTTATTTCAAATTTGAAGTTGATTTCAGTTCAAAT
TATTATTGTGTAATTCCTGTTGCATTCTTCCAAGCTGGTTCTTCCATTACAATGAGAAAT
GCTGATCCAACTCATCCAAATACAAATAGTGATGCTTTCTTATCAACTGTTGATAATGTT
TATGTCACTTCTTTTACTTCTTCTGATGCTGGGGTTATTATTTTAATGGTTGTTCATTGG
TATTATTCCAAAAAAATAAAGTAATAAAAAAATA

>SHC223P.Seq
AAAATTTTAATTATTGTATATAATATTTTTAAATATATATATTAATATATATTAATATAT
TAATATTAATAAAATGAAAATATTATATTCATTATTATTAATATCAAGTATAATTTTAAA
TACTGTATTAAATATTTCATCACAAGTATATGATCAACGTATTTTGGCATTAAAAGGAAA
ATGTTATTTAGATTCAAATAAAAATGCATTGTATGATGAAGGTGAACCAGGATTTGGAAA
TGTTGAAATTTCTTTAGGAAATGCAACTGATTATAAGGGAAATAAATATCCAATACGTTA
TTCATCTGCTGATGGTACATTTATATATGATGGATTAACTCAACGTACAACTTATGAATT
TACATTTACCAATCCAAAAGATTATTACTTTGAAACCTATGAAAAGACATTGGCTTGTCC
ACCAGGTGTACCATGTATTCGTCCAACGGTTTCAGCTGATAATTTGGCAGCAGATCTTTT
AGTTGCAAATACCTTTGCCTATAACATTCCATTGATTAAACAAGTTGGTGTTTCATCAGG
CTCATCTATACTTACAGTTAGAATTAGTGTTGATTTGGGTTATGTTGAGCAAACTCAACC
ACCAACTCAACCACCTACTCAACCACCTACTCAACCACCAACACCTCCA----------G
AGGTAAATGTGGTAGTCTTGTTGGTGCCGGAGTTGATCAAAAATCACTCACTGGTCTTAG
ATATGGTCCAGGTAATGGTAATTTCTTTGATAATCATAAAACAGGTCTTCATGACTATAC
CAATGCTTTTGGTGCAACTAAAGGTGGTCGTGATATTATTGTTACAACTGGTTTCGATTA
CTATGAAGCATTTGAAGGTTCAATTCGTTGGTATAATGCAACCTCTGGTAAACAACTAAA
AGGTTACTCTTTATATATAACCTCTTCAAATGGTCGTACCCCATCTCCAACCTTTGGTAA
ACAAAATGGTCTTGGTGATATTACTTCAGTGTATGATTCTTCTCTTCCACAATATCGTGT
TGGTAGAGTTTGGCTTGATATAAATGGTAATGGTATTCAAGATGCTGGTGAACCTGGTAT
TTCAGGAATCACAGTATTCTTAATCTATGGTCAACAATCAACTCCAACAAGTCAAACTGT
ATCCGATTCAAATGGTTATTTCAAATTTGAAGTTGATTTCAGTTCAAATTATTATTGTGT
AATTCCTGTTGCATTCTTCCAAGCTGGTTCTTCCATTACAATGAGAAATGCTGATCCAAC
TCATCCAAATACAAATAGTGATGCTTTCTTATCAACTGTTGATAATGTTTATGTCACTTC
TTTTACTTCTTCTGATGCTGGGGTTATTATTTTAATGGTTGTTCATTGGTATTATTCCAA
AAAAATAAAGTAATAAAAAAATA