>SHC139 (SHC139Q) /CSM/SH/SHC1-B/SHC139Q.Seq.d/
ATTAATTTTAATTTATATAAATTACCTTATTACTTAAAGATGACAACAAATAATCCATTT
GATAAAGTAAAGGATGTATTAAAATCACAAGATCAAACTTATAATTTTTATAATCTTTCA
AAACTTCAAGACCCAAGAATTGAAAAATTACCATACTCCATTAGAATTCTTTTGGAATCA
GCAGTTCGTAATTGTGATAACTTTGAAGTACACGAAAAAGATGTTGAAAATATCTTAAAT
TGGGAAAACACTGCAAATAAAGTTGAAATTCCTTTCAAACCAGCAAGAGTATTATTACAA
GATTTTACTGGTGTACCAGCAGTTGTTGATCTTGCAGCAATGAGAGATGCCATGAAAAGA
TTAGGTGGTGATCCAGCAAAAATTAACCCACTTGTTCCAGTTGATTTAGTTATTGATCAC
TCTGTTCAAGTTGATGTATCAAGAACTGTTGATGCTTTAGAGCAAAATCAAAAGATTGAA
TTCCATAGAAATCATGAAAGATTTTCATTTTTAAAATGGGGTGCTCAAGCATTTGATGGA
TTATTTATTGCTCCACCTGGAAGTGGTATTGTACATCAAGTAAATTTAGAGTATATXXXX
XXXXXXTTTAAGACTATGGAATTAACCGTTTCAAAGAGACCTGCCATTACAAATGCTTAT
TGTCTTTTGAATCTTGGTGATAGTATTACCACTGATCATATTTCACCAGCCGGTAACATC
AATAGAAAATCATCAGCCGCTCGTTATTTAGAGTCCAAAGGTGTAAAACCAGAAGATTTT
AACACCTATGGATCTCGTAGAGGTAATGATGAAATTATGGTTAGAGGTACCTTTGCCAAT
ACTCGTATTGTTAATAAATTGGCTCCAGCCGTTGGTCCACAAACAACCTATGTACCAACT
GGTGAGTTAATGTTTATTTCAGATGCTGCAGAAAAATATCAATCAGAAGGTCATCAACTC
ATTGTTTTGGCTGGTAGTGATTATGGCTCTGGTAGTTCTAGAGATTGGGCTGCCAAAGGT
CCATACCTTCAAGGAATTAAGTGTGTCATTGCCATCTCATTTGAACGTATTCATAGATCC
AATCTCGTTGGTATGGGTATTATCCCATTGCAATTCCAACCAGGTCAAAATGCCTCTACT
TTGGGTCTCACTGGTAAAGAACAATTCAATATCGAGTTACCAACTGATAAATCCCTCATT
AAAACTGGTCAAACCGTAAAGGTTACCACTAATTGTGGTAAATCTTTTGAAACTATCCTT
CGTTTCGATACTCCAATCGAAGTTGAATATTGGGCAAATAATGGTATCTTATCTTATGTT
TTAAGAAAACTTTTACATTAATTTCTTTTTTTAA

INFNLYKLPYYLKMTTNNPFDKVKDVLKSQDQTYNFYNLSKLQDPRIEKLPYSIRILLES
AVRNCDNFEVHEKDVENILNWENTANKVEIPFKPARVLLQDFTGVPAVVDLAAMRDAMKR
LGGDPAKINPLVPVDLVIDHSVQVDVSRTVDALEQNQKIEFHRNHERFSFLKWGAQAFDG
LFIAPPGSGIVHQVNLEY---

---FKTMELTVSKRPAITNAYCLLNLGDSITTDHISPAGNINRKSSAARYLESKGVKPED
FNTYGSRRGNDEIMVRGTFANTRIVNKLAPAVGPQTTYVPTGELMFISDAAEKYQSEGHQ
LIVLAGSDYGSGSSRDWAAKGPYLQGIKCVIAISFERIHRSNLVGMGIIPLQFQPGQNAS
TLGLTGKEQFNIELPTDKSLIKTGQTVKVTTNCGKSFETILRFDTPIEVEYWANNGILSY
VLRKLLH*flfl

Frame A:
INFNLYKLPYYLKMTTNNPFDKVKDVLKSQDQTYNFYNLSKLQDPRIEKLPYSIRILLES
AVRNCDNFEVHEKDVENILNWENTANKVEIPFKPARVLLQDFTGVPAVVDLAAMRDAMKR
LGGDPAKINPLVPVDLVIDHSVQVDVSRTVDALEQNQKIEFHRNHERFSFLKWGAQAFDG
LFIAPPGSGIVHQVNLEY---

---FKTMELTVSKRPAITNAYCLLNLGDSITTDHISPAGNINRKSSAARYLESKGVKPED
FNTYGSRRGNDEIMVRGTFANTRIVNKLAPAVGPQTTYVPTGELMFISDAAEKYQSEGHQ
LIVLAGSDYGSGSSRDWAAKGPYLQGIKCVIAISFERIHRSNLVGMGIIPLQFQPGQNAS
TLGLTGKEQFNIELPTDKSLIKTGQTVKVTTNCGKSFETILRFDTPIEVEYWANNGILSY
VLRKLLH*flfl

Frame B:
liliyinylit*r*qqiihlik*rmy*nhkikliifiifqnfktqelknyhtpleffwnq
qfvivitlkytkkmlkis*igktlqiklkflsnqqeyyykillvyqqllilqq*emp*kd
*vviqqklthlfqli*llitlfklmyqellml*skikrlnsieimkdfhf*ngvlkhlmd
ylllhlevvlyik*i*si---

---lrlwn*pfqrdlplqmlivf*ilvivlpliifhqpvtsienhqplvi*spkv*nqki
ltpmdlvevmmklwlevplpilvllinwlqplvhkqpmyqlvs*clfqmlqkninqkvin
slfwlvvimalvvleiglpkvhtfkelsvslpshlnvfidpislvwvlshcnsnqvkmpl
lwvslvknnsissyqlinpslklvkp*rlplivvnllklsfvsilqsklnigqimvsylm
f*enfyinfff*

Frame C:
*f*fi*itlllkddnk*si**skgcikitrsnl*fl*sfktsrpkn*kitilh*nsfgis
ss*l**l*strkrc*kylklgkhck*s*nsfqtsksiitrfywctssc*scsnercheki
rw*sskn*ptcss*fsy*slcss*ciknc*cfrakskd*ip*ks*kififkmgcssi*wi
iycstwkwyctsskfrvy---

---*dyginrfketchykcllsfesw**yyh*syftsr*hq*kiisrslfrvqrcktrrf
*hlwis*r***nyg*rylcqysyc**igssrwstnnlctnw*vnvyfrccrkisirrsst
hcfgw**lwlw*f*rlgcqrsipsrn*vchchli*tys*iqsrwygyypiaiptrskcly
fgshw*rtiqyrvtn**iph*nwsnrkgyh*lw*if*nypsfrysnrs*ilgk*wylilc
fkktftlisff

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHC139 (SHC139Q) /CSM/SH/SHC1-B/SHC139Q.Seq.d/                       2607   0.0
SHK673 (SHK673Q) /CSM/SH/SHK6-D/SHK673Q.Seq.d/                       1465   0.0
CHL805 (CHL805Q) /CSM/CH/CHL8-A/CHL805Q.Seq.d/                       1465   0.0
AHN120 (AHN120Q) /CSM/AH/AHN1-A/AHN120Q.Seq.d/                       1465   0.0
AHL516 (AHL516Q) /CSM/AH/AHL5-A/AHL516Q.Seq.d/                       1465   0.0
AHJ164 (AHJ164Q) /CSM/AH/AHJ1-C/AHJ164Q.Seq.d/                       1465   0.0
AHD743 (AHD743Q) /CSM/AH/AHD7-B/AHD743Q.Seq.d/                       1465   0.0
AHD311 (AHD311Q) /CSM/AH/AHD3-A/AHD311Q.Seq.d/                       1465   0.0
AHD230 (AHD230Q) /CSM/AH/AHD2-B/AHD230Q.Seq.d/                       1465   0.0
AHD150 (AHD150Q) /CSM/AH/AHD1-C/AHD150Q.Seq.d/                       1465   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF194945|AF194945.1 Nicotiana tabacum cytosolic aconitase (ACO) mRNA, complete cds.    70   6e-35  6
AY250116|AY250116.1 Lycopersicon pennellii aconitase mRNA, partial cds.    60   5e-26  5
AY250115|AY250115.1 Lycopersicon pennellii aconitase mRNA, partial cds.    60   2e-21  5
BE579347|BE579347.1 kq26c09.y1 TBN95TM-SSR Strongyloides stercoralis cDNA 5' similar to WP:ZK455.1 CE03812 PROBABLE ACONITATE HYDRATASE, CYTOPLASMIC ;, mRNA sequence.    64   2e-20  3
BI925204|BI925204.1 EST545093 tomato flower, buds 0-3 mm Lycopersicon esculentum cDNA clone cTOA25H12 5' end, mRNA sequence.    58   6e-19  3
BI930520|BI930520.1 EST550409 tomato flower, 8 mm to preanthesis buds Lycopersicon esculentum cDNA clone cTOC16J14 5' end, mRNA sequence.    58   7e-19  3
CO910019|CO910019.1 BJ02055G09 BJ02 Capsicum annuum cDNA 5', mRNA sequence.    58   1e-18  4
CK265172|CK265172.1 EST711250 potato abiotic stress cDNA library Solanum tuberosum cDNA clone POABX20 5' end, mRNA sequence.    58   1e-17  4
AY062772|AY062772.1 Arabidopsis thaliana Unknown protein (At4g35830; F4B14.100) mRNA, complete cds.    46   5e-17  6
BQ511380|BQ511380.2 EST618795 Generation of a set of potato cDNA clones for microarray analyses mixed potato tissues Solanum tuberosum cDNA clone STMHR19 5' end, mRNA sequence.    58   8e-16  3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54X73) RecName: Full=Probable cytoplasmic aconitate hydratase;...   490   e-137
AB236788_1(AB236788|pid:none) Trifolium pratense RNA for putativ...   311   3e-83
BT060820_1(BT060820|pid:none) Zea mays full-length cDNA clone ZM...   309   1e-82
AP008209_256(AP008209|pid:none) Oryza sativa (japonica cultivar-...   309   1e-82
BT060665_1(BT060665|pid:none) Zea mays full-length cDNA clone ZM...   308   4e-82
(P49608) RecName: Full=Aconitate hydratase, cytoplasmic;        ...   305   3e-81
AY050485_1(AY050485|pid:none) Prunus avium putative aconitase mR...   304   4e-81
(Q23500) RecName: Full=Probable cytoplasmic aconitate hydratase;...   303   9e-81
(Q9SIB9) RecName: Full=Aconitate hydratase 2, mitochondrial;    ...   302   2e-80
DQ294230_1(DQ294230|pid:none) Glossina morsitans morsitans TC165...   302   2e-80

 psg: 0.75  gvh: 0.20  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 36.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: extracellular, including cell wall
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: plasma membrane

>> prediction for SHC139 is cyt

>SHC139F.Seq
ATTAATTTTAATTTATATAAATTACCTTATTACTTAAAGATGACAACAAATAATCCATTT
GATAAAGTAAAGGATGTATTAAAATCACAAGATCAAACTTATAATTTTTATAATCTTTCA
AAACTTCAAGACCCAAGAATTGAAAAATTACCATACTCCATTAGAATTCTTTTGGAATCA
GCAGTTCGTAATTGTGATAACTTTGAAGTACACGAAAAAGATGTTGAAAATATCTTAAAT
TGGGAAAACACTGCAAATAAAGTTGAAATTCCTTTCAAACCAGCAAGAGTATTATTACAA
GATTTTACTGGTGTACCAGCAGTTGTTGATCTTGCAGCAATGAGAGATGCCATGAAAAGA
TTAGGTGGTGATCCAGCAAAAATTAACCCACTTGTTCCAGTTGATTTAGTTATTGATCAC
TCTGTTCAAGTTGATGTATCAAGAACTGTTGATGCTTTAGAGCAAAATCAAAAGATTGAA
TTCCATAGAAATCATGAAAGATTTTCATTTTTAAAATGGGGTGCTCAAGCATTTGATGGA
TTATTTATTGCTCCACCTGGAAGTGGTATTGTACATCAAGTAAATTTAGAGTATATNNNN
NNNNNN

>SHC139Z.Seq
NNNNNNNNNNTTTAAGACTATGGAATTAACCGTTTCAAAGAGACCTGCCATTACAAATGC
TTATTGTCTTTTGAATCTTGGTGATAGTATTACCACTGATCATATTTCACCAGCCGGTAA
CATCAATAGAAAATCATCAGCCGCTCGTTATTTAGAGTCCAAAGGTGTAAAACCAGAAGA
TTTTAACACCTATGGATCTCGTAGAGGTAATGATGAAATTATGGTTAGAGGTACCTTTGC
CAATACTCGTATTGTTAATAAATTGGCTCCAGCCGTTGGTCCACAAACAACCTATGTACC
AACTGGTGAGTTAATGTTTATTTCAGATGCTGCAGAAAAATATCAATCAGAAGGTCATCA
ACTCATTGTTTTGGCTGGTAGTGATTATGGCTCTGGTAGTTCTAGAGATTGGGCTGCCAA
AGGTCCATACCTTCAAGGAATTAAGTGTGTCATTGCCATCTCATTTGAACGTATTCATAG
ATCCAATCTCGTTGGTATGGGTATTATCCCATTGCAATTCCAACCAGGTCAAAATGCCTC
TACTTTGGGTCTCACTGGTAAAGAACAATTCAATATCGAGTTACCAACTGATAAATCCCT
CATTAAAACTGGTCAAACCGTAAAGGTTACCACTAATTGTGGTAAATCTTTTGAAACTAT
CCTTCGTTTCGATACTCCAATCGAAGTTGAATATTGGGCAAATAATGGTATCTTATCTTA
TGTTTTAAGAAAACTTTTACATTAATTTCTTTTTTTAA

>SHC139P.Seq
ATTAATTTTAATTTATATAAATTACCTTATTACTTAAAGATGACAACAAATAATCCATTT
GATAAAGTAAAGGATGTATTAAAATCACAAGATCAAACTTATAATTTTTATAATCTTTCA
AAACTTCAAGACCCAAGAATTGAAAAATTACCATACTCCATTAGAATTCTTTTGGAATCA
GCAGTTCGTAATTGTGATAACTTTGAAGTACACGAAAAAGATGTTGAAAATATCTTAAAT
TGGGAAAACACTGCAAATAAAGTTGAAATTCCTTTCAAACCAGCAAGAGTATTATTACAA
GATTTTACTGGTGTACCAGCAGTTGTTGATCTTGCAGCAATGAGAGATGCCATGAAAAGA
TTAGGTGGTGATCCAGCAAAAATTAACCCACTTGTTCCAGTTGATTTAGTTATTGATCAC
TCTGTTCAAGTTGATGTATCAAGAACTGTTGATGCTTTAGAGCAAAATCAAAAGATTGAA
TTCCATAGAAATCATGAAAGATTTTCATTTTTAAAATGGGGTGCTCAAGCATTTGATGGA
TTATTTATTGCTCCACCTGGAAGTGGTATTGTACATCAAGTAAATTTAGAGTATAT----
------TTTAAGACTATGGAATTAACCGTTTCAAAGAGACCTGCCATTACAAATGCTTAT
TGTCTTTTGAATCTTGGTGATAGTATTACCACTGATCATATTTCACCAGCCGGTAACATC
AATAGAAAATCATCAGCCGCTCGTTATTTAGAGTCCAAAGGTGTAAAACCAGAAGATTTT
AACACCTATGGATCTCGTAGAGGTAATGATGAAATTATGGTTAGAGGTACCTTTGCCAAT
ACTCGTATTGTTAATAAATTGGCTCCAGCCGTTGGTCCACAAACAACCTATGTACCAACT
GGTGAGTTAATGTTTATTTCAGATGCTGCAGAAAAATATCAATCAGAAGGTCATCAACTC
ATTGTTTTGGCTGGTAGTGATTATGGCTCTGGTAGTTCTAGAGATTGGGCTGCCAAAGGT
CCATACCTTCAAGGAATTAAGTGTGTCATTGCCATCTCATTTGAACGTATTCATAGATCC
AATCTCGTTGGTATGGGTATTATCCCATTGCAATTCCAACCAGGTCAAAATGCCTCTACT
TTGGGTCTCACTGGTAAAGAACAATTCAATATCGAGTTACCAACTGATAAATCCCTCATT
AAAACTGGTCAAACCGTAAAGGTTACCACTAATTGTGGTAAATCTTTTGAAACTATCCTT
CGTTTCGATACTCCAATCGAAGTTGAATATTGGGCAAATAATGGTATCTTATCTTATGTT
TTAAGAAAACTTTTACATTAATTTCTTTTTTTAA