>SHC130 (SHC130Q) /CSM/SH/SHC1-B/SHC130Q.Seq.d/
XXXXXXXXXXCACCTGCTGTAAACTCTCATAAGATTGTAACTCAAATGGGAAATGTTGAA
AATAATGAAATTACCAATGAACAAGGTGCCATTGAACAAGCTCAACATTTACAACGTATT
GAAAATGGTGAAGATATCGATGAGTATGAAGCTGCCCAACATGAAGTTGCCTTTTTAGAT
GAAAATGAACAAGATGCTGCTAAATTACAAAATGAAATGGACCAATTAGAACTCTTACTT
GAAGAGAATGAAGAAGAACAATTTTCAAATAAAAATTTACAACAACTTAATGAGAATGAA
AATCAACAACAACAACAAGGACAACAACAAGTTCAAGCAACAAATGTTGAACAACAAATC
GTTGAACAACTTAAAGTTATCAAAGAATTTCAAAGACAAGATCAACAAAAGAAACAAAAA
ATTCAACAAGAGAACGATGCTGTCTACTTGAGTGATGTTGAAAAAGCTCAACTTGAATTG
GATGCTGAATTGGCTAAAAATAATAAACAAGAGAATCAAGATGAGAATTTAGTTCAAGAG
AAACAACAATCTCCTGACCAAATTAAAAATCAATTAAAAAATA

---PAVNSHKIVTQMGNVENNEITNEQGAIEQAQHLQRIENGEDIDEYEAAQHEVAFLDE
NEQDAAKLQNEMDQLELLLEENEEEQFSNKNLQQLNENENQQQQQGQQQVQATNVEQQIV
EQLKVIKEFQRQDQQKKQKIQQENDAVYLSDVEKAQLELDAELAKNNKQENQDENLVQEK
QQSPDQIKNQLKN

Frame A:
---tcckls*dcnsngkc*k**nyq*trch*tsstftty*kw*ryr*v*scpt*sclfr*
k*trcc*itk*ngpirtlt*re*rrtifk*kfttt**e*ksttttrtttsssnkc*ttnr
*tt*syqrisktrstketknstrercclle*c*ksst*igc*ig*k**tresr*efssre
ttis*pn*ksikk


Frame B:
---PAVNSHKIVTQMGNVENNEITNEQGAIEQAQHLQRIENGEDIDEYEAAQHEVAFLDE
NEQDAAKLQNEMDQLELLLEENEEEQFSNKNLQQLNENENQQQQQGQQQVQATNVEQQIV
EQLKVIKEFQRQDQQKKQKIQQENDAVYLSDVEKAQLELDAELAKNNKQENQDENLVQEK
QQSPDQIKNQLKN


Frame C:
---ll*tlirl*lkwemlkimklpmnkvplnklniynvlkmvkismsmklpnmklpf*mk
mnkmllnykmkwtn*nsylkrmkknnfqikiynnlmrmkinnnnkdnnkfkqqmlnnksl
nnlklsknfkdkinkrnkkfnkrtmlst*vmlkklnlnwmlnwlkiinkrikmri*fkrn
nnlltklkin*ki

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHD537 (SHD537Q) /CSM/SH/SHD5-B/SHD537Q.Seq.d/                        579   e-164
SHC130 (SHC130Q) /CSM/SH/SHC1-B/SHC130Q.Seq.d/                        579   e-164
VHP272 (VHP272Q) /CSM/VH/VHP2-C/VHP272Q.Seq.d/                        571   e-162
SHL894 (SHL894Q) /CSM/SH/SHL8-D/SHL894Q.Seq.d/                        571   e-162
SHL890 (SHL890Q) /CSM/SH/SHL8-D/SHL890Q.Seq.d/                        571   e-162
SHL879 (SHL879Q) /CSM/SH/SHL8-D/SHL879Q.Seq.d/                        571   e-162
SHL875 (SHL875Q) /CSM/SH/SHL8-D/SHL875Q.Seq.d/                        571   e-162
SHL866 (SHL866Q) /CSM/SH/SHL8-C/SHL866Q.Seq.d/                        571   e-162
SHL865 (SHL865Q) /CSM/SH/SHL8-C/SHL865Q.Seq.d/                        571   e-162
SHL857 (SHL857Q) /CSM/SH/SHL8-C/SHL857Q.Seq.d/                        571   e-162

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U25143|U25143.1 Dictyostelium discoideum cAMP-inducible prespore protein gene, complete cds.   571   0.0    2
AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.   127   3e-25  1
AE014832|AE014832.1 Plasmodium falciparum 3D7 chromosome 10 section 4 of 7 of the complete sequence.    36   0.049  6
CB855513|CB855513.1 ri40a10.y1 Meloidogyne chitwoodi J2 pDNR LIB Meloidogyne chitwoodi cDNA 5', mRNA sequence.    32   0.20   3
DT633626|DT633626.1 EST1148557 Normalized pine embryo library, Lib_D Pinus taeda cDNA clone PIMFW90 3' end, mRNA sequence.    48   0.20   1
DT364098|DT364098.1 JGI_ANNN9278.rev ANNN Pimephales promelas Whole (L) Pimephales promelas cDNA clone ANNN9278 3', mRNA sequence.    48   0.20   1
DT347978|DT347978.1 JGI_CAAX10802.fwd CAAX Pimephales promelas testis 7-8 month adults, males and females pooled (L) Pimephales promelas cDNA clone CAAX10802 5', mRNA sequence.    48   0.20   1
DT309975|DT309975.1 JGI_CAAX1517.fwd CAAX Pimephales promelas testis 7-8 month adults, males and females pooled (L) Pimephales promelas cDNA clone CAAX1517 5', mRNA sequence.    48   0.20   1
DT309974|DT309974.1 JGI_CAAX1517.rev CAAX Pimephales promelas testis 7-8 month adults, males and females pooled (L) Pimephales promelas cDNA clone CAAX1517 3', mRNA sequence.    48   0.20   1
DR686823|DR686823.1 EST1076901 Normalized pine embryo library, Lib_D Pinus taeda cDNA clone PWABK24 3' end, mRNA sequence.    48   0.20   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P54682) RecName: Full=cAMP-inducible prespore protein D7; Flags...   231   1e-59
AF314648_2(AF314648|pid:none) Plasmodium vivax CG9 (cg9) gene, c...    35   1.1
(Q54F33) RecName: Full=Uncharacterized protein DDB_G0291143;           35   1.9
CP000806_1851(CP000806|pid:none) Cyanothece sp. ATCC 51142 circu...    33   4.2
AC009323_10(AC009323|pid:none) Arabidopsis thaliana chromosome I...    33   5.5
AP009240_1649(AP009240|pid:none) Escherichia coli SE11 DNA, comp...    33   5.5
AY059831_1(AY059831|pid:none) Arabidopsis thaliana Unknown prote...    33   5.5
AE016819_111(AE016819|pid:none) Ashbya gossypii (= Eremothecium ...    33   7.2
(P47048) RecName: Full=Uncharacterized protein YJL049W;  &AY6926...    33   7.2
AP009049_1790(AP009049|pid:none) Clostridium kluyveri NBRC 12016...    32   9.4

 psg: 0.81  gvh: 0.16  alm: 0.58  top: 0.53  tms: 0.00  mit: 0.26  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 28.0 %: cytoplasmic
	 16.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane

>> prediction for SHC130 is nuc

>SHC130Z.Seq
NNNNNNNNNNCACCTGCTGTAAACTCTCATAAGATTGTAACTCAAATGGGAAATGTTGAA
AATAATGAAATTACCAATGAACAAGGTGCCATTGAACAAGCTCAACATTTACAACGTATT
GAAAATGGTGAAGATATCGATGAGTATGAAGCTGCCCAACATGAAGTTGCCTTTTTAGAT
GAAAATGAACAAGATGCTGCTAAATTACAAAATGAAATGGACCAATTAGAACTCTTACTT
GAAGAGAATGAAGAAGAACAATTTTCAAATAAAAATTTACAACAACTTAATGAGAATGAA
AATCAACAACAACAACAAGGACAACAACAAGTTCAAGCAACAAATGTTGAACAACAAATC
GTTGAACAACTTAAAGTTATCAAAGAATTTCAAAGACAAGATCAACAAAAGAAACAAAAA
ATTCAACAAGAGAACGATGCTGTCTACTTGAGTGATGTTGAAAAAGCTCAACTTGAATTG
GATGCTGAATTGGCTAAAAATAATAAACAAGAGAATCAAGATGAGAATTTAGTTCAAGAG
AAACAACAATCTCCTGACCAAATTAAAAATCAATTAAAAAATA