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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHB826
Library SH
(Link to library)
Clone ID SHB826
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16330-1
Original site URL
Representative seq. ID SHB826P
(Link to Original site)
Representative DNA sequence 
>SHB826 (SHB826Q) /CSM/SH/SHB8-B/SHB826Q.Seq.d/
CTGTTGGCCTACTCAATCGTTGCAATGTCAGCTTGTGCCATTGGTTCCACTAGNGGTTAT
GATGAAATCTTCCCAAAAACAATTGATCTAGTAAATGAAACTAGAGTTTACAATACCAAT
GTTAATCTTTCAAGTGGTATTCTACCAATTAGATCATTATTATCAAAGATACACTTGGAA
CTATCATGTAATGGTTTTGATGAAATTCATATTCATCAAGATGGTAATTTAATTTTCATT
CAAAGATATTCACCAAAATTAAATGAATCAATATTTATGGTTGCTCATTGTTCATTCTCA
AATCAACAACAACAACAACAGCAAGATAATGATAAAGAATTACATGTACCTTGTAAAATT
TCACAATTTTTATTTGGTGCAAAGATTACCAATACCCATATCAATAGAAATGATTGGAAT
ACAAATACAACCAAATTGAATGGTATTGAAGTAGATTGTGAAATTCATTATCATGATAAA
GTTTTAAATCAATTTTGTCAAATTCATGAATATAACAATGAGAATGGTGCACTTTGTACA
ACGTTGAAATTAAATAACTTTAAAGCTGGTTCCATTTTATTCTTTAGAGGTGAAATTCCA
XXXXXXXXXXCAAATATTTGTGTTGCAATGTCATTTGCNCCAAAGTTATTCAATGATCAA
CATGCAAATAAATGTATTGATATCATTAGAAATTCATTGGTTGGTCCAATCGGTATGAAA
ANTNTTGATCCAAATGATCCAAATTATCATGGTAACTATGATAATTCATCAGATTNTGGT
TATAAACCAACATCAAGAGGTTTCAATTATCATAATGGTCCTGAATGGGTTTGGTGTTAT
GGTTTNTTTTTGGAATCAATTGTACAATTTAAACAGTTCCCTTCGATTTCAACAAAGAAT
CATTTAATCAATTCATTATTAATTAAACATAAACTTTACATTCAAAATGATAAACGTTTT
TCATTACCAGAGTTAACTAATNGTGAAGGTAGTTTTTGTAAAGATAGTTGTGATTNTCAA
GCTTGGTCAANTGCAACAATTTTAGCTNCCCCTTATCNATTATCAATTAATAAAAATTAA
ATATTCATTTTTT
sequence update 2002.10.25
Translated Amino Acid sequence 
LLAYSIVAMSACAIGSTXGYDEIFPKTIDLVNETRVYNTNVNLSSGILPIRSLLSKIHLE
LSCNGFDEIHIHQDGNLIFIQRYSPKLNESIFMVAHCSFSNQQQQQQQDNDKELHVPCKI
SQFLFGAKITNTHINRNDWNTNTTKLNGIEVDCEIHYHDKVLNQFCQIHEYNNENGALCT
TLKLNNFKAGSILFFRGEIP---

---NICVAMSFAPKLFNDQHANKCIDIIRNSLVGPIGMKXXDPNDPNYHGNYDNSSDXGY
KPTSRGFNYHNGPEWVWCYGXFLESIVQFKQFPSISTKNHLINSLLIKHKLYIQNDKRFS
LPELTNXEGSFCKDSCDXQAWSXATILAXPYXLSINKN*ifif
Translated Amino Acid sequence (All Frames) 
Frame A:
LLAYSIVAMSACAIGSTXGYDEIFPKTIDLVNETRVYNTNVNLSSGILPIRSLLSKIHLE
LSCNGFDEIHIHQDGNLIFIQRYSPKLNESIFMVAHCSFSNQQQQQQQDNDKELHVPCKI
SQFLFGAKITNTHINRNDWNTNTTKLNGIEVDCEIHYHDKVLNQFCQIHEYNNENGALCT
TLKLNNFKAGSILFFRGEIP---

---qifvlqchlxqsysminmqinvlisleihwlvqsv*kxliqmiqiimvtmiihqixv
inqhqevsiiimvlngfgvmvxfwnqlynlnsslrfqqrii*sihy*lninftfkminvf
hyqs*lxvkvvfvkivvixklgqxqqf*lplixyqlikikysff

Frame B:
cwptqslqcqlvplvplxvmmkssqkqli**mkleftipmlifqvvfyqldhyyqrytwn
yhvmvlmkfifikmvi*fsfkdihqn*mnqylwllivhsqinnnnnskimiknymylvkf
hnfylvqrlpipisiemigiqiqpn*mvlk*ivkfiimikf*infvkfmnitmrmvhfvq
r*n*itlklvpfyslevkf---

---kylccnvicxkviq*stck*my*yh*kfigwsnryexx*sk*sklsw*l**firxwl
*tnikrfqls*ws*mglvlwfxfgincti*tvpfdfnkesfnqfiin*t*tlhsk**tff
itrvn*x*r*fl*r*l*xsslvxcnnfsxplsiin**klnihf

Frame C:
vgllnrcnvslchwfh*xl**nlpknn*ssk*n*slqyqc*sfkwystn*iiiikdtlgt
im*wf**nsyssrw*fnfhskiftkik*iniygcslfilkstttttar***ritctl*nf
tifiwckdyqypyq*k*leykynqiewy*srl*nsls**sfksilsns*i*q*ewctlyn
veik*l*swfhfil*r*ns---

---NICVAMSFAPKLFNDQHANKCIDIIRNSLVGPIGMKXXDPNDPNYHGNYDNSSDXGY
KPTSRGFNYHNGPEWVWCYGXFLESIVQFKQFPSISTKNHLINSLLIKHKLYIQNDKRFS
LPELTNXEGSFCKDSCDXQAWSXATILAXPYXLSINKN*ifif
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHB826 (SHB826Q) /CSM/SH/SHB8-B/SHB826Q.Seq.d/                       1366   0.0
SHB250 (SHB250Q) /CSM/SH/SHB2-C/SHB250Q.Seq.d/                        829   0.0
AHP129 (AHP129Q) /CSM/AH/AHP1-B/AHP129Q.Seq.d/                        829   0.0
CHM605 (CHM605Q) /CSM/CH/CHM6-A/CHM605Q.Seq.d/                        827   0.0
AHD862 (AHD862Q) /CSM/AH/AHD8-C/AHD862Q.Seq.d/                        823   0.0
CHI519 (CHI519Q) /CSM/CH/CHI5-A/CHI519Q.Seq.d/                        821   0.0
SHL636 (SHL636Q) /CSM/SH/SHL6-B/SHL636Q.Seq.d/                        817   0.0
VHG332 (VHG332Q) /CSM/VH/VHG3-B/VHG332Q.Seq.d/                        813   0.0
AHJ766 (AHJ766Q) /CSM/AH/AHJ7-C/AHJ766Q.Seq.d/                        811   0.0
VHD429 (VHD429Q) /CSM/VH/VHD4-B/VHD429Q.Seq.d/                        809   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

CR802444|CR802444.1 GR0AAA21DE04RM1 INRA BAC Bos taurus genomic clone INRAb_427E09, DNAsequence.    54   0.006  1
AC115612|AC115612.2 Dictyostelium discoideum chromosome 2 map 6245135-6357017 strain AX4, complete sequence.    44   0.021  8
CR380953|CR380953.1 Candida glabrata strain CBS138 chromosome G complete sequence.    52   0.025  1
BX957285|BX957285.8 Zebrafish DNA sequence from clone CH211-160M18 in linkage group 10.    50   0.090  3
CE177742|CE177742.1 tigr-gss-dog-17000326745699 Dog Library Canis familiaris genomic, genomic survey sequence.    50   0.099  1
AL929299|AL929299.10 Zebrafish DNA sequence from clone BUSM1-SL6.    50   0.099  1
AC159407|AC159407.1 Trypanosoma brucei chromosome 8 clone RPCI93-12O16, complete sequence.    38   0.22   2
AC068396|AC068396.4 Homo sapiens chromosome 15 clone RP11-348K3 map 15, WORKING DRAFT SEQUENCE, 2 ordered pieces.    48   0.39   1
AL390072|AL390072.18 Human DNA sequence *** SEQUENCING CANCELLED *** from clone  RP11-530P13.    48   0.39   1
AC162187|AC162187.2 Bos taurus clone CH240-117B1, *** SEQUENCING IN PROGRESS ***, 34 unordered pieces.    48   0.39   1
dna update 2005.12.29
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

EF660736_1(EF660736|pid:none) Equus caballus amylo-1, 6-glucosid...   127   8e-28
EF660736_5(EF660736|pid:none) Equus caballus amylo-1, 6-glucosid...   127   8e-28
BC167247_1(BC167247|pid:none) Synthetic construct Mus musculus c...   126   1e-27
M85168_1(M85168|pid:none) Human glycogen debranching enzyme mRNA...   126   1e-27
U84011_1(U84011|pid:none) Human glycogen debranching enzyme isof...   126   1e-27
AK028914_1(AK028914|pid:none) Mus musculus 10 days neonate skin ...   126   1e-27
AE016818_70(AE016818|pid:none) Ashbya gossypii (= Eremothecium g...   125   3e-27
(P35573) RecName: Full=Glycogen debranching enzyme; AltName: Ful...   124   4e-27
BC091979_1(BC091979|pid:none) Danio rerio im:7145503, mRNA (cDNA...   122   3e-26
CR380953_424(CR380953|pid:none) Candida glabrata strain CBS138 c...   117   5e-25
protein update 2009. 4.15
PSORT 

 psg: 0.93  gvh: 0.59  alm: 0.35  top: 0.33  tms: 0.00  mit: 0.26  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 28.0 %: cytoplasmic
	 28.0 %: nuclear
	 16.0 %: mitochondrial
	 12.0 %: extracellular, including cell wall
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: endoplasmic reticulum

>> prediction for SHB826 is cyt
5' end seq. ID SHB826F
5' end seq. 
>SHB826F.Seq
CTGTTGGCCTACTCAATCGTTGCAATGTCAGCTTGTGCCATTGGTTCCACTAGNGGTTAT
GATGAAATCTTCCCAAAAACAATTGATCTAGTAAATGAAACTAGAGTTTACAATACCAAT
GTTAATCTTTCAAGTGGTATTCTACCAATTAGATCATTATTATCAAAGATACACTTGGAA
CTATCATGTAATGGTTTTGATGAAATTCATATTCATCAAGATGGTAATTTAATTTTCATT
CAAAGATATTCACCAAAATTAAATGAATCAATATTTATGGTTGCTCATTGTTCATTCTCA
AATCAACAACAACAACAACAGCAAGATAATGATAAAGAATTACATGTACCTTGTAAAATT
TCACAATTTTTATTTGGTGCAAAGATTACCAATACCCATATCAATAGAAATGATTGGAAT
ACAAATACAACCAAATTGAATGGTATTGAAGTAGATTGTGAAATTCATTATCATGATAAA
GTTTTAAATCAATTTTGTCAAATTCATGAATATAACAATGAGAATGGTGCACTTTGTACA
ACGTTGAAATTAAATAACTTTAAAGCTGGTTCCATTTTATTCTTTAGAGGTGAAATTCCA
NNNNNNNNNN
Length of 5' end seq. 610
3' end seq. ID SHB826Z
3' end seq. 
>SHB826Z.Seq
NNNNNNNNNNCAAATATTTGTGTTGCAATGTCATTTGCNCCAAAGTTATTCAATGATCAA
CATGCAAATAAATGTATTGATATCATTAGAAATTCATTGGTTGGTCCAATCGGTATGAAA
ANTNTTGATCCAAATGATCCAAATTATCATGGTAACTATGATAATTCATCAGATTNTGGT
TATAAACCAACATCAAGAGGTTTCAATTATCATAATGGTCCTGAATGGGTTTGGTGTTAT
GGTTTNTTTTTGGAATCAATTGTACAATTTAAACAGTTCCCTTCGATTTCAACAAAGAAT
CATTTAATCAATTCATTATTAATTAAACATAAACTTTACATTCAAAATGATAAACGTTTT
TCATTACCAGAGTTAACTAATNGTGAAGGTAGTTTTTGTAAAGATAGTTGTGATTNTCAA
GCTTGGTCAANTGCAACAATTTTAGCTNCCCCTTATCNATTATCAATTAATAAAAATTAA
ATATTCATTTTTT
Length of 3' end seq. 493
Connected seq. ID SHB826P
Connected seq. 
>SHB826P.Seq
CTGTTGGCCTACTCAATCGTTGCAATGTCAGCTTGTGCCATTGGTTCCACTAGNGGTTAT
GATGAAATCTTCCCAAAAACAATTGATCTAGTAAATGAAACTAGAGTTTACAATACCAAT
GTTAATCTTTCAAGTGGTATTCTACCAATTAGATCATTATTATCAAAGATACACTTGGAA
CTATCATGTAATGGTTTTGATGAAATTCATATTCATCAAGATGGTAATTTAATTTTCATT
CAAAGATATTCACCAAAATTAAATGAATCAATATTTATGGTTGCTCATTGTTCATTCTCA
AATCAACAACAACAACAACAGCAAGATAATGATAAAGAATTACATGTACCTTGTAAAATT
TCACAATTTTTATTTGGTGCAAAGATTACCAATACCCATATCAATAGAAATGATTGGAAT
ACAAATACAACCAAATTGAATGGTATTGAAGTAGATTGTGAAATTCATTATCATGATAAA
GTTTTAAATCAATTTTGTCAAATTCATGAATATAACAATGAGAATGGTGCACTTTGTACA
ACGTTGAAATTAAATAACTTTAAAGCTGGTTCCATTTTATTCTTTAGAGGTGAAATTCCA
----------CAAATATTTGTGTTGCAATGTCATTTGCNCCAAAGTTATTCAATGATCAA
CATGCAAATAAATGTATTGATATCATTAGAAATTCATTGGTTGGTCCAATCGGTATGAAA
ANTNTTGATCCAAATGATCCAAATTATCATGGTAACTATGATAATTCATCAGATTNTGGT
TATAAACCAACATCAAGAGGTTTCAATTATCATAATGGTCCTGAATGGGTTTGGTGTTAT
GGTTTNTTTTTGGAATCAATTGTACAATTTAAACAGTTCCCTTCGATTTCAACAAAGAAT
CATTTAATCAATTCATTATTAATTAAACATAAACTTTACATTCAAAATGATAAACGTTTT
TCATTACCAGAGTTAACTAATNGTGAAGGTAGTTTTTGTAAAGATAGTTGTGATTNTCAA
GCTTGGTCAANTGCAACAATTTTAGCTNCCCCTTATCNATTATCAATTAATAAAAATTAA
ATATTCATTTTTT
Length of connected seq. 1083
Full length Seq ID -
Full length Seq. -
Length of full length seq. -