>SHB712 (SHB712Q) /CSM/SH/SHB7-A/SHB712Q.Seq.d/
CTGTTGGCCTACTGGNAAAAAACACTACCACTATATTTTCCCACGCTGTAAAAACAAAAA
AACAAAAAACAAAAAAAATAAAAAGAAAAAAAATCTAAGAATCAAATACAAAACTTTAAA
AACAATGTCACTCTTCAAGAAGAATTACAAATTTGTTTGCTCCAAGTCAACAATCCAATT
TAATTGAATGTAATGGTTATTTTTATCACTTTTATAAAGGACAATGGGTTTGTTTATTTA
ATGATTGTGAATTAGTTATTCATAAATTTGAAAATTATAAATACCAAATTCAAGTTACAA
GAATTGTAGATCAAGAACAAGAAAATGAAAGAAGAAAACAAAGGGAAGCAGAAGGTTTAG
AAGATGAAGAGGAAGAAGAGAATCTTGTAAATTATTTTGAATTTCCAGTTACAAATGGAT
TAGAATTATATAATGAAATTGGACCATTAGGTAATTTCTCATTAAGTTGGAAAGGTACAA
ATACAAGATTCTTATTCGAATTTGATAGTAATAATAATAATAACAATAATAACAATAATA
ATAATGAAAGTATTGAAACAACAGGAAATCAATTAAAGAGAATATTATGTCAATGTATTT
ATGAATCAAXXXXXXXXXXGGCTACAATGGTAATAATATGTTTTTAGTGNATCCACGTGA
ATCAAAGAATAAAGTTACCGTTAAATTTCATGGTGGTTCAAATCCAAAATCTGTAACTAC
ATGTGCTGCCACCACTGGTAGTGGTCAAATTGCTTTTGGTACGTCTAAAGGTGAAATCAA
ACTTTTCAGCAAAACTCAATTCGATCAAAATAAACGTTCCGTCACCTCTTCCGACCCTTT
GGGTGCTATTGCTCGTTCACGTACTACCCTACCGGGTATTGGTGATCCAATCGTTGGTAT
CGATGTCACCAAAGATGGTAAATGGATCGTTGCAACCTGTAAACAATATATCATGGTCAT
ACCAGCTTCACTCAAAGATGGTAGTAGTGGTTTTGAAGATCGTTTGGGTGCTCGTCGTCC
TTCACCAAAACGTTTAATTTTAAAACCTCAAGATATTAAACGTATGGGTGGTGTCGTCAA
TTTCACACCAGCCAAATTCAATATCGTTGGTGATGAACAATCTGAAACCTCCATTCTCAC
AAGCACCGGCAGTTTCCTTATCACTTGGAATTTTAGAAAAATTAAACAAAACATTTTGGA
CGTTTATCAAATTAAACAATTCCAAGATGGTGTTGTAGCTGAACAATTTAAATCAAATAG
AGATAATTCAATAGTTGTTACTTTCCCTGATAATGNATTTATCAAAAAAGAAAAAAAAAC
AAAATAA

vgllxkntttifshavktkkqktkkikrkki*esntkl*KQCHSSRRITNLFAPSQQSNL
IECNGYFYHFYKGQWVCLFNDCELVIHKFENYKYQIQVTRIVDQEQENERRKQREAEGLE
DEEEEENLVNYFEFPVTNGLELYNEIGPLGNFSLSWKGTNTRFLFEFDSNNNNNNNNNNN
NESIETTGNQLKRILCQCIYES---

---GYNGNNMFLVXPRESKNKVTVKFHGGSNPKSVTTCAATTGSGQIAFGTSKGEIKLFS
KTQFDQNKRSVTSSDPLGAIARSRTTLPGIGDPIVGIDVTKDGKWIVATCKQYIMVIPAS
LKDGSSGFEDRLGARRPSPKRLILKPQDIKRMGGVVNFTPAKFNIVGDEQSETSILTSTG
SFLITWNFRKIKQNILDVYQIKQFQDGVVAEQFKSNRDNSIVVTFPDNXFIKKEKKTK*

Frame A:
llaywxktlplyfptl*kqknkkqkk*kekksknqiqnfknnvtlqeelqicllqvnnpi
*lnvmvifitfikdngfvylmivn*lfinlkiintkfklqel*iknkkmkeenkgkqkv*
kmkrkkril*iilnfqlqmd*nyimkldh*vish*vgkvqiqdsysnliviiiitiitii
imkvlkqqein*reyyvnvfmnq---

---GYNGNNMFLVXPRESKNKVTVKFHGGSNPKSVTTCAATTGSGQIAFGTSKGEIKLFS
KTQFDQNKRSVTSSDPLGAIARSRTTLPGIGDPIVGIDVTKDGKWIVATCKQYIMVIPAS
LKDGSSGFEDRLGARRPSPKRLILKPQDIKRMGGVVNFTPAKFNIVGDEQSETSILTSTG
SFLITWNFRKIKQNILDVYQIKQFQDGVVAEQFKSNRDNSIVVTFPDNXFIKKEKKTK*

Frame B:
cwptgkkhyhyifprcknkktknkknkkkknlrikyktlktmslfkknykfvcskstiqf
n*m*wlflsll*rtmglfi**l*isys*i*kl*ipnssykncrsrtrk*kkktkgsrrfr
r*rgrrescklf*issykwirii**nwtir*fliklerykykiliri*******q**q**
**ky*nnrksikenimsmyl*i---

---atmviicf*xihvnqriklplnfmvvqiqnl*lhvlpplvvvklllvrlkvksnfsa
klnsikinvpsplptlwvlllvhvlpyrvlviqslvsmspkmvngslqpvnniswsyqlh
skmvvvvlkivwvlvvlhqnv*f*nlkilnvwvvssishqpnsislvmnnlkppfsqapa
vslslgileklnktfwtfiklnnskmvl*lnnlnqieiiq*lllslimxlskkkkkqn

Frame C:
vgllxkntttifshavktkkqktkkikrkki*esntkl*KQCHSSRRITNLFAPSQQSNL
IECNGYFYHFYKGQWVCLFNDCELVIHKFENYKYQIQVTRIVDQEQENERRKQREAEGLE
DEEEEENLVNYFEFPVTNGLELYNEIGPLGNFSLSWKGTNTRFLFEFDSNNNNNNNNNNN
NESIETTGNQLKRILCQCIYES---

---lqw**yvfsxst*ike*syr*iswwfkskicnymcchhw*wsncfwyv*r*nqtfqq
nsirsk*tfrhlfrpfgcycsftyyptgyw*snrwyrchqrw*mdrcnl*tiyhghtsft
qrw**wf*rsfgcsssftktfnfktsry*tygwcrqfhtsqiqyrw**ti*nlhshkhrq
fpyhlef*kn*tkhfgrlsn*tiprwccs*ti*ik*r*fnscyfp**xiyqkrkknki

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHB712 (SHB712Q) /CSM/SH/SHB7-A/SHB712Q.Seq.d/                       1431   0.0
CHM543 (CHM543Q) /CSM/CH/CHM5-B/CHM543Q.Seq.d/                       1334   0.0
AHH419 (AHH419Q) /CSM/AH/AHH4-A/AHH419Q.Seq.d/                       1334   0.0
AHH330 (AHH330Q) /CSM/AH/AHH3-B/AHH330Q.Seq.d/                       1334   0.0
AHA610 (AHA610Q) /CSM/AH/AHA6-A/AHA610Q.Seq.d/                       1334   0.0
AHM787 (AHM787Q) /CSM/AH/AHM7-D/AHM787Q.Seq.d/                       1326   0.0
AHD848 (AHD848Q) /CSM/AH/AHD8-B/AHD848Q.Seq.d/                       1326   0.0
SLB212 (SLB212Q) /CSM/SL/SLB2-A/SLB212Q.Seq.d/                       1318   0.0
AHN469 (AHN469Q) /CSM/AH/AHN4-C/AHN469Q.Seq.d/                       1318   0.0
AHC648 (AHC648Q) /CSM/AH/AHC6-B/AHC648Q.Seq.d/                       1306   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.  1334   0.0    1
AC068203|AC068203.2 Homo sapiens chromosome 4 clone RP11-683C4 map 4, WORKING DRAFT SEQUENCE, 18 unordered pieces.    52   0.030  1
AC104814|AC104814.5 Homo sapiens BAC clone RP11-683C4 from 4, complete sequence.    52   0.030  1
AC093790|AC093790.3 Homo sapiens BAC clone RP11-250F14 from 4, complete sequence.    52   0.030  1
CO731046|CO731046.1 JBW004G03.b_019.abi Pineapple green mature fruit cDNA library Ananas comosus cDNA clone JBW004G03 similar to unknown protein, mRNA sequence.    50   0.12   1
CO730778|CO730778.1 JBW001D09.b_073.abi Pineapple green mature fruit cDNA library Ananas comosus cDNA clone JBW001D09 similar to unknown protein, mRNA sequence.    50   0.12   1
AP008101|AP008101.1 Lotus corniculatus var. japonicus genomic DNA, clone:LjT22H07, TM1476.    50   0.12   1
CP000016|CP000016.1 Candidatus Blochmannia pennsylvanicus str. BPEN, complete genome.    34   0.17   15
AC090446|AC090446.26 Baboon cytomegalovirus OCOM4-37 clone bacmv, WORKING DRAFT SEQUENCE, 23 unordered pieces.    48   0.47   1
CR589906|CR589906.2 Human DNA sequence from clone WI2-3068F15 on chromosome 1.    48   0.47   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116986_49(AC116986|pid:none) Dictyostelium discoideum chromoso...   449   e-124
EF612188_1(EF612188|pid:none) Dictyostelium minutum unknown (Dm0...   334   4e-90
AE017345_303(AE017345|pid:none) Cryptococcus neoformans var. neo...   167   1e-39
(Q1MTR3) RecName: Full=Vacuolar import and degradation protein 2...   147   8e-34
FM992693_11(FM992693|pid:none) Candida dubliniensis CD36 chromos...   139   2e-31
CU633457_256(CU633457|pid:none) Podospora anserina genomic DNA c...   137   1e-30
CR382130_222(CR382130|pid:none) Yarrowia lipolytica strain CLIB1...   135   3e-30
AM920435_379(AM920435|pid:none) Penicillium chrysogenum Wisconsi...   134   5e-30
AP007151_1191(AP007151|pid:none) Aspergillus oryzae RIB40 genomi...   132   3e-29
CR382135_411(CR382135|pid:none) Debaryomyces hansenii strain CBS...   125   2e-27

 psg: 0.84  gvh: 0.41  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.45  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: mitochondrial
	 24.0 %: cytoplasmic
	 16.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for SHB712 is mit

>SHB712F.Seq
CTGTTGGCCTACTGGNAAAAAACACTACCACTATATTTTCCCACGCTGTAAAAACAAAAA
AACAAAAAACAAAAAAAATAAAAAGAAAAAAAATCTAAGAATCAAATACAAAACTTTAAA
AACAATGTCACTCTTCAAGAAGAATTACAAATTTGTTTGCTCCAAGTCAACAATCCAATT
TAATTGAATGTAATGGTTATTTTTATCACTTTTATAAAGGACAATGGGTTTGTTTATTTA
ATGATTGTGAATTAGTTATTCATAAATTTGAAAATTATAAATACCAAATTCAAGTTACAA
GAATTGTAGATCAAGAACAAGAAAATGAAAGAAGAAAACAAAGGGAAGCAGAAGGTTTAG
AAGATGAAGAGGAAGAAGAGAATCTTGTAAATTATTTTGAATTTCCAGTTACAAATGGAT
TAGAATTATATAATGAAATTGGACCATTAGGTAATTTCTCATTAAGTTGGAAAGGTACAA
ATACAAGATTCTTATTCGAATTTGATAGTAATAATAATAATAACAATAATAACAATAATA
ATAATGAAAGTATTGAAACAACAGGAAATCAATTAAAGAGAATATTATGTCAATGTATTT
ATGAATCAANNNNNNNNNN

>SHB712Z.Seq
NNNNNNNNNNGGCTACAATGGTAATAATATGTTTTTAGTGNATCCACGTGAATCAAAGAA
TAAAGTTACCGTTAAATTTCATGGTGGTTCAAATCCAAAATCTGTAACTACATGTGCTGC
CACCACTGGTAGTGGTCAAATTGCTTTTGGTACGTCTAAAGGTGAAATCAAACTTTTCAG
CAAAACTCAATTCGATCAAAATAAACGTTCCGTCACCTCTTCCGACCCTTTGGGTGCTAT
TGCTCGTTCACGTACTACCCTACCGGGTATTGGTGATCCAATCGTTGGTATCGATGTCAC
CAAAGATGGTAAATGGATCGTTGCAACCTGTAAACAATATATCATGGTCATACCAGCTTC
ACTCAAAGATGGTAGTAGTGGTTTTGAAGATCGTTTGGGTGCTCGTCGTCCTTCACCAAA
ACGTTTAATTTTAAAACCTCAAGATATTAAACGTATGGGTGGTGTCGTCAATTTCACACC
AGCCAAATTCAATATCGTTGGTGATGAACAATCTGAAACCTCCATTCTCACAAGCACCGG
CAGTTTCCTTATCACTTGGAATTTTAGAAAAATTAAACAAAACATTTTGGACGTTTATCA
AATTAAACAATTCCAAGATGGTGTTGTAGCTGAACAATTTAAATCAAATAGAGATAATTC
AATAGTTGTTACTTTCCCTGATAATGNATTTATCAAAAAAGAAAAAAAAACAAAATAA

>SHB712P.Seq
CTGTTGGCCTACTGGNAAAAAACACTACCACTATATTTTCCCACGCTGTAAAAACAAAAA
AACAAAAAACAAAAAAAATAAAAAGAAAAAAAATCTAAGAATCAAATACAAAACTTTAAA
AACAATGTCACTCTTCAAGAAGAATTACAAATTTGTTTGCTCCAAGTCAACAATCCAATT
TAATTGAATGTAATGGTTATTTTTATCACTTTTATAAAGGACAATGGGTTTGTTTATTTA
ATGATTGTGAATTAGTTATTCATAAATTTGAAAATTATAAATACCAAATTCAAGTTACAA
GAATTGTAGATCAAGAACAAGAAAATGAAAGAAGAAAACAAAGGGAAGCAGAAGGTTTAG
AAGATGAAGAGGAAGAAGAGAATCTTGTAAATTATTTTGAATTTCCAGTTACAAATGGAT
TAGAATTATATAATGAAATTGGACCATTAGGTAATTTCTCATTAAGTTGGAAAGGTACAA
ATACAAGATTCTTATTCGAATTTGATAGTAATAATAATAATAACAATAATAACAATAATA
ATAATGAAAGTATTGAAACAACAGGAAATCAATTAAAGAGAATATTATGTCAATGTATTT
ATGAATCAA----------GGCTACAATGGTAATAATATGTTTTTAGTGNATCCACGTGA
ATCAAAGAATAAAGTTACCGTTAAATTTCATGGTGGTTCAAATCCAAAATCTGTAACTAC
ATGTGCTGCCACCACTGGTAGTGGTCAAATTGCTTTTGGTACGTCTAAAGGTGAAATCAA
ACTTTTCAGCAAAACTCAATTCGATCAAAATAAACGTTCCGTCACCTCTTCCGACCCTTT
GGGTGCTATTGCTCGTTCACGTACTACCCTACCGGGTATTGGTGATCCAATCGTTGGTAT
CGATGTCACCAAAGATGGTAAATGGATCGTTGCAACCTGTAAACAATATATCATGGTCAT
ACCAGCTTCACTCAAAGATGGTAGTAGTGGTTTTGAAGATCGTTTGGGTGCTCGTCGTCC
TTCACCAAAACGTTTAATTTTAAAACCTCAAGATATTAAACGTATGGGTGGTGTCGTCAA
TTTCACACCAGCCAAATTCAATATCGTTGGTGATGAACAATCTGAAACCTCCATTCTCAC
AAGCACCGGCAGTTTCCTTATCACTTGGAATTTTAGAAAAATTAAACAAAACATTTTGGA
CGTTTATCAAATTAAACAATTCCAAGATGGTGTTGTAGCTGAACAATTTAAATCAAATAG
AGATAATTCAATAGTTGTTACTTTCCCTGATAATGNATTTATCAAAAAAGAAAAAAAAAC
AAAATAA