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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHB543
Library SH
(Link to library)
Clone ID SHB543
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16471-1
Original site URL
Representative seq. ID SHB543P
(Link to Original site)
Representative DNA sequence 
>SHB543 (SHB543Q) /CSM/SH/SHB5-B/SHB543Q.Seq.d/
CTGTTGGCCTACTGGAATAAATTTACTATCAAATAACTATTTTTTCTTTTCTTTCTTTTT
AAAGTTTAAATAATAGAAATAATAAAAATTAATATTAAAAAAAAGGAACGTTAAAAAAAA
AAXXXXXXXXXXAACAAGATGCCATTGAACAAGCTCAACATTTACAACGTATTGAAAATG
GTGAAGATATCGATGAGTATGAAGCTGCCCNACATGAAGTTGCCTTTTTAGATGAAAATG
AACAAGATGCTGNTAAATTACAAAATGAAATGGACCAATTAGAANATCTTACTTGAAGAG
AATGAAGAAGAACAATTTTCAAATAAAAATTTNCAACAACTTAATGAGAATGAAAATCAA
CAACAACAACAAGGACAACAACAAGTTCAAGCAACAAATGTTGAACAACAAATCGTTGAA
CAACTTAAAGTTATCAAAGAATTTCAAAGACAAGATCAACAAAAGAAACAAAAAATTCAA
CAAGAGAACGATGCTGTCTACTTGAGTGATGTTGANANAGCTCAACTTGAATTGGATGCT
GAATTGGCTAAAAATAATAAACAAGAGAATCAAGATGAGAATTTAGTTCAAGAGAAGACA
GACAATNTCNTGACCAAATTAAAAATCAATTAAAAAATATTCAACATGAACAACAAATCC
AAGAACNCCNAGATAAATTAAATCAAGAAAAAAATCNACNATTATTAGAACAAGAACAAA
ATCAAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
cwptginllsnnyfffsfflkfk**k**KLILKKRNVKKK---

---QDAIEQAQHLQRIENGEDIDEYEAAXHEVAFLDENEQDAXKLQNEMDQLEXLT*re*
rrtifk*kfxtt**e*ksttttrtttsssnkc*ttnr*tt*syqrisktrstketknstr
ercclle*c*xsst*igc*ig*k**tresr*efssredrqxxdqiknqlkniqheqqiqe
xxdklnqeknxxlleqeqnqk
Translated Amino Acid sequence (All Frames) 
Frame A:
llaywnkftik*lfflfflfkv*iieiikinikkker*kk---

---nkmplnklniynvlkmvkismsmklpxmklpf*mkmnkmlxnykmkwtn*xilleen
eeeqfsnknxqqlnenenqqqqqgqqqvqatnveqqiveqlkvikefqrqdqqkkqkiqq
endavylsdvxxaqleldaelaknnkqenqdenlvqektdnxxtklkin*kifnmnnksk
nxxin*ikkkixxy*nknkik

Frame B:
cwptginllsnnyfffsfflkfk**k**KLILKKRNVKKK---

---trch*tsstftty*kw*ryr*v*scpt*sclfr*k*trcx*itk*ngpirxsylkrm
kknnfqikixnnlmrmkinnnnkdnnkfkqqmlnnkslnnlklsknfkdkinkrnkkfnk
rtmlst*vmlxxlnlnwmlnwlkiinkrikmri*fkrrqtxs*pn*ksikkyst*ttnpr
tpr*iksrkkstiirtrtksk

Frame C:
vglle*iyyqitifsflsf*slnnrnnkn*y*kkgtlkkk---

---QDAIEQAQHLQRIENGEDIDEYEAAXHEVAFLDENEQDAXKLQNEMDQLEXLT*re*
rrtifk*kfxtt**e*ksttttrtttsssnkc*ttnr*tt*syqrisktrstketknstr
ercclle*c*xsst*igc*ig*k**tresr*efssredrqxxdqiknqlkniqheqqiqe
xxdklnqeknxxlleqeqnqk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHB543 (SHB543Q) /CSM/SH/SHB5-B/SHB543Q.Seq.d/                        541   e-153
CHJ185 (CHJ185Q) /CSM/CH/CHJ1-D/CHJ185Q.Seq.d/                        519   e-146
VHG846 (VHG846Q) /CSM/VH/VHG8-B/VHG846Q.Seq.d/                        502   e-141
SLI803 (SLI803Q) /CSM/SL/SLI8-A/SLI803Q.Seq.d/                        502   e-141
SLI655 (SLI655Q) /CSM/SL/SLI6-C/SLI655Q.Seq.d/                        502   e-141
SLH374 (SLH374Q) /CSM/SL/SLH3-D/SLH374Q.Seq.d/                        502   e-141
SLH142 (SLH142Q) /CSM/SL/SLH1-B/SLH142Q.Seq.d/                        502   e-141
SLG381 (SLG381Q) /CSM/SL/SLG3-D/SLG381Q.Seq.d/                        502   e-141
SLC743 (SLC743Q) /CSM/SL/SLC7-B/SLC743Q.Seq.d/                        502   e-141
SLB365 (SLB365Q) /CSM/SL/SLB3-C/SLB365Q.Seq.d/                        502   e-141
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U25143|U25143.1 Dictyostelium discoideum cAMP-inducible prespore protein gene, complete cds.   357   0.0    6
AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.    68   3e-17  2
BH137529|BH137529.1 ENTPA17TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    34   0.080  3
AC117070|AC117070.2 Dictyostelium discoideum chromosome 2 map 2097701-2233941 strain AX4, complete sequence.    34   0.13   7
DT633626|DT633626.1 EST1148557 Normalized pine embryo library, Lib_D Pinus taeda cDNA clone PIMFW90 3' end, mRNA sequence.    48   0.26   1
DT364098|DT364098.1 JGI_ANNN9278.rev ANNN Pimephales promelas Whole (L) Pimephales promelas cDNA clone ANNN9278 3', mRNA sequence.    48   0.26   1
DT347978|DT347978.1 JGI_CAAX10802.fwd CAAX Pimephales promelas testis 7-8 month adults, males and females pooled (L) Pimephales promelas cDNA clone CAAX10802 5', mRNA sequence.    48   0.26   1
DT309975|DT309975.1 JGI_CAAX1517.fwd CAAX Pimephales promelas testis 7-8 month adults, males and females pooled (L) Pimephales promelas cDNA clone CAAX1517 5', mRNA sequence.    48   0.26   1
DT309974|DT309974.1 JGI_CAAX1517.rev CAAX Pimephales promelas testis 7-8 month adults, males and females pooled (L) Pimephales promelas cDNA clone CAAX1517 3', mRNA sequence.    48   0.26   1
DT102344|DT102344.1 JGI_ANNN5669.rev ANNN Pimephales promelas Whole (L) Pimephales promelas cDNA clone ANNN5669 3', mRNA sequence.    48   0.26   1
dna update 2005.12.25
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P54682) RecName: Full=cAMP-inducible prespore protein D7; Flags...    99   1e-19
protein update 2008.11.13
PSORT 

 psg: 0.46  gvh: 0.16  alm: 0.65  top: 0.53  tms: 0.00  mit: 0.25  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: nuclear
	 32.0 %: cytoplasmic
	  8.0 %: mitochondrial
	  4.0 %: plasma membrane

>> prediction for SHB543 is nuc
5' end seq. ID SHB543F
5' end seq. 
>SHB543F.Seq
CTGTTGGCCTACTGGAATAAATTTACTATCAAATAACTATTTTTTCTTTTCTTTCTTTTT
AAAGTTTAAATAATAGAAATAATAAAAATTAATATTAAAAAAAAGGAACGTTAAAAAAAA
AANNNNNNNNNN
Length of 5' end seq. 132
3' end seq. ID SHB543Z
3' end seq. 
>SHB543Z.Seq
NNNNNNNNNNAACAAGATGCCATTGAACAAGCTCAACATTTACAACGTATTGAAAATGGT
GAAGATATCGATGAGTATGAAGCTGCCCNACATGAAGTTGCCTTTTTAGATGAAAATGAA
CAAGATGCTGNTAAATTACAAAATGAAATGGACCAATTAGAANATCTTACTTGAAGAGAA
TGAAGAAGAACAATTTTCAAATAAAAATTTNCAACAACTTAATGAGAATGAAAATCAACA
ACAACAACAAGGACAACAACAAGTTCAAGCAACAAATGTTGAACAACAAATCGTTGAACA
ACTTAAAGTTATCAAAGAATTTCAAAGACAAGATCAACAAAAGAAACAAAAAATTCAACA
AGAGAACGATGCTGTCTACTTGAGTGATGTTGANANAGCTCAACTTGAATTGGATGCTGA
ATTGGCTAAAAATAATAAACAAGAGAATCAAGATGAGAATTTAGTTCAAGAGAAGACAGA
CAATNTCNTGACCAAATTAAAAATCAATTAAAAAATATTCAACATGAACAACAAATCCAA
GAACNCCNAGATAAATTAAATCAAGAAAAAAATCNACNATTATTAGAACAAGAACAAAAT
CAAAAA
Length of 3' end seq. 606
Connected seq. ID SHB543P
Connected seq. 
>SHB543P.Seq
CTGTTGGCCTACTGGAATAAATTTACTATCAAATAACTATTTTTTCTTTTCTTTCTTTTT
AAAGTTTAAATAATAGAAATAATAAAAATTAATATTAAAAAAAAGGAACGTTAAAAAAAA
AA----------AACAAGATGCCATTGAACAAGCTCAACATTTACAACGTATTGAAAATG
GTGAAGATATCGATGAGTATGAAGCTGCCCNACATGAAGTTGCCTTTTTAGATGAAAATG
AACAAGATGCTGNTAAATTACAAAATGAAATGGACCAATTAGAANATCTTACTTGAAGAG
AATGAAGAAGAACAATTTTCAAATAAAAATTTNCAACAACTTAATGAGAATGAAAATCAA
CAACAACAACAAGGACAACAACAAGTTCAAGCAACAAATGTTGAACAACAAATCGTTGAA
CAACTTAAAGTTATCAAAGAATTTCAAAGACAAGATCAACAAAAGAAACAAAAAATTCAA
CAAGAGAACGATGCTGTCTACTTGAGTGATGTTGANANAGCTCAACTTGAATTGGATGCT
GAATTGGCTAAAAATAATAAACAAGAGAATCAAGATGAGAATTTAGTTCAAGAGAAGACA
GACAATNTCNTGACCAAATTAAAAATCAATTAAAAAATATTCAACATGAACAACAAATCC
AAGAACNCCNAGATAAATTAAATCAAGAAAAAAATCNACNATTATTAGAACAAGAACAAA
ATCAAAAA
Length of connected seq. 718
Full length Seq ID -
Full length Seq. -
Length of full length seq. -