>SHB466 (SHB466Q) /CSM/SH/SHB4-C/SHB466Q.Seq.d/
GCCACATCCTATTCGGCTAATAATGGTCAAAATGAAGAATATACATTTGATGCATTTGAA
GATGGTTCATCTAAATTGGTTGGAGAGCAACAATTAACTAAACTTAATCAATTTGCAGTT
GATTATGCACAACAAATTTGGAAGATAGAAGAAGCATTAGATGAAACATTAAATGAAGTA
TGGGATATAAATATAGATCCAGTTTCAATTTATAATAAACCTCATGAACAATTAGATTTA
ATTGAATTGGTTAAAACTGATCATAAAATTTTCAATAAAGTAATATTAGTATTCTCTTCA
ATTTGTAATCAAACTAGAATTTTAAAAGAAACAGCAGAAAGTAAATTTTATTCACCATTA
ACAGTATTTGGAGAGATTACAGGTGAATCATCAGAAGGTGATGTACAAATTGAAGTTGGT
AAATTATTACCATTTATGATTGATTTATCAGCATTTGTAAATAGATGTTATTCATTAATT
AGAAATATTATTAGTCAATTTGCATCGATCTATCAATCACAAAAGAATATACATACACAA
TTCTTTAAGAATGTTCATTTACAAGCTGTCTATTATTCAATGATTGATATATTTTCAGTA
TTAATAAATTTAGATAGTATAATTACACAAAATACTGCTTTAXXXXXXXXXXGGTGACAG
AGTTGGAAAGAGACATTAGACAATTGGGTGTCTCCGAGACTGGTTTAACCTTTTTAGATC
ATTTTAGACTTTTAATCACTCATATTGGTAATGCTATGGGTTATATTAGATTGGTCAGAT
CCGGTGGTTTACACTATTGTAGTAATGCTATCAAATTCGTACCAGACTTGAAAAAGATTC
CAAAATTCCAAGATTTAACCTCAAAAGATGCCCTCTCACCTGAAACAATTCAAGCTAGTA
CCAATTTGGATTCAATCATTCATAATCTTTCAAATAATTTATCAGAGGGTACTGAATACT
TTAAGATGTTGGTAAACGTTTTCGCTACAGAGTTTCGAAATATCGCTAATCAACATTTAA
AGAATTTCTATATCATCGTACCTGCCTTGGCCTCAAATTACATCGATCATATGATCAATA
GTAAAGATAAACTCTTTAAGAAATCAAAAGCAATCGGTGCTGAAGCTTTATTATTCACTG
ATGATGGCTTTGCAATTGGTTTGGCTTATATCTTAAAATTATTAGATCAAAATAAAGATT
TCGATTCTCTTCATTGGTTCGATCGTATTCAATTGAAATGTGAAACNGATCAAAAGAGAA
TGTTGGCTGAAGCAAATGCAAAAGGTATCAAAGAGGATCAAAATCATTTAGCAATTAAAA
AGATTCAAAATTTCCAACAAGAATANAATTATTTAAATTCTTTCTCGGTTCAAGAATTTT
CTTAAAG

ATSYSANNGQNEEYTFDAFEDGSSKLVGEQQLTKLNQFAVDYAQQIWKIEEALDETLNEV
WDINIDPVSIYNKPHEQLDLIELVKTDHKIFNKVILVFSSICNQTRILKETAESKFYSPL
TVFGEITGESSEGDVQIEVGKLLPFMIDLSAFVNRCYSLIRNIISQFASIYQSQKNIHTQ
FFKNVHLQAVYYSMIDIFSVLINLDSIITQNTAL---

---VTELERDIRQLGVSETGLTFLDHFRLLITHIGNAMGYIRLVRSGGLHYCSNAIKFVP
DLKKIPKFQDLTSKDALSPETIQASTNLDSIIHNLSNNLSEGTEYFKMLVNVFATEFRNI
ANQHLKNFYIIVPALASNYIDHMINSKDKLFKKSKAIGAEALLFTDDGFAIGLAYILKLL
DQNKDFDSLHWFDRIQLKCETDQKRMLAEANAKGIKEDQNHLAIKKIQNFQQEXNYLNSF
SVQEFS*

Frame A:
ATSYSANNGQNEEYTFDAFEDGSSKLVGEQQLTKLNQFAVDYAQQIWKIEEALDETLNEV
WDINIDPVSIYNKPHEQLDLIELVKTDHKIFNKVILVFSSICNQTRILKETAESKFYSPL
TVFGEITGESSEGDVQIEVGKLLPFMIDLSAFVNRCYSLIRNIISQFASIYQSQKNIHTQ
FFKNVHLQAVYYSMIDIFSVLINLDSIITQNTAL---

---gdrvgkrh*tigclrdwfnlfrsf*tfnhsyw*cygly*igqirwftll**cyqirt
rlekdskiprfnlkrcplt*nnss*yqfgfnhs*sfk*firgy*il*dvgkrfryrvsky
r*stfkeflyhrtclglklhrsydq**r*tl*eiksnrc*sfiih**wlcnwfglylkii
rsk*rfrfsslvrsysiem*nxskenvg*skckryqrgsksfsn*kdskfptrixlfkff
lgsriflk

Frame B:
phpirlimvkmknihlmhlkmvhlnwlesnn*lnlinlqlimhnkfgr*kkh*mkh*mky
gi*i*iqfqfiinlmnn*i*lnwlkliikfsik*y*yslqfviklef*kkqqkvnfihh*
qylerlqvnhqkvmyklklvnyyhl*liyqhl*idvih*leillvnlhrsinhkriyihn
slrmfiyklsiiq*liyfqy**i*iv*lhkill---

---VTELERDIRQLGVSETGLTFLDHFRLLITHIGNAMGYIRLVRSGGLHYCSNAIKFVP
DLKKIPKFQDLTSKDALSPETIQASTNLDSIIHNLSNNLSEGTEYFKMLVNVFATEFRNI
ANQHLKNFYIIVPALASNYIDHMINSKDKLFKKSKAIGAEALLFTDDGFAIGLAYILKLL
DQNKDFDSLHWFDRIQLKCETDQKRMLAEANAKGIKEDQNHLAIKKIQNFQQEXNYLNSF
SVQEFS*

Frame C:
hilfg**wsk*riyi*ci*rwfi*igwratin*t*sics*lcttnledrrsir*nik*sm
gykyrssfnl**ts*tirfn*ig*n*s*nfq*snisilfnl*sn*nfkrnsrk*ilftin
siwrdyr*iirr*ctn*sw*iitiyd*fisick*mlfin*kyy*sicidlsitkeytyti
l*ecsftscllfnd*yifsinkfr*ynytkycf---

---*qswketldnwvsprlv*pf*iildf*slilvmlwvildwsdpvvytivvmlsnsyq
t*krfqnski*pqkmpshlkqfklvpiwiqsfiifqiiyqrvlntlrcw*tfslqsfeis
lini*risissylpwpqitsii*sivkinslrnqkqsvlklyyslmmalqlvwlis*ny*
ikikisilfigsivfn*nvkxikrecwlkqmqkvskrikii*qlkrfkisnknxii*ils
rfknflk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHB466 (SHB466Q) /CSM/SH/SHB4-C/SHB466Q.Seq.d/                       2678   0.0
VHK424 (VHK424Q) /CSM/VH/VHK4-A/VHK424Q.Seq.d/                       1421   0.0
SHB243 (SHB243Q) /CSM/SH/SHB2-B/SHB243Q.Seq.d/                       1421   0.0
VHI518 (VHI518Q) /CSM/VH/VHI5-A/VHI518Q.Seq.d/                       1419   0.0
VHA684 (VHA684Q) /CSM/VH/VHA6-D/VHA684Q.Seq.d/                       1413   0.0
VHI654 (VHI654Q) /CSM/VH/VHI6-C/VHI654Q.Seq.d/                       1405   0.0
VHJ568 (VHJ568Q) /CSM/VH/VHJ5-C/VHJ568Q.Seq.d/                       1403   0.0
AHN345 (AHN345Q) /CSM/AH/AHN3-B/AHN345Q.Seq.d/                       1403   0.0
SHK609 (SHK609Q) /CSM/SH/SHK6-A/SHK609Q.Seq.d/                       1400   0.0
SHG190 (SHG190Q) /CSM/SH/SHG1-D/SHG190Q.Seq.d/                       1398   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BU746157|BU746157.1 CH3#002_C07T7 Canine heart normalized cDNA Library in pBluescriptCanis familiaris cDNA clone CH3#002_C07 5', mRNA sequence.    38   7e-04  3
DN287505|DN287505.1 1241755 MARC 7BOV Bos taurus cDNA 3', mRNA sequence.    34   0.003  4
DR027063|DR027063.1 EST00469 Feline cDNA, uterus library Felis catus cDNA clone E0491 5' similar to Homo sapiens KIAA1033 protein, mRNA sequence.    40   0.003  4
BM802928|BM802928.1 AGENCOURT_6457255 NIH_MGC_88 Homo sapiens cDNA clone IMAGE:5560300 5', mRNA sequence.    40   0.014  3
AK113047|AK113047.1 Ciona intestinalis cDNA, clone:ciad002a21, full insert sequence.    48   0.030  2
BU746156|BU746156.1 CH3#002_C07T3 Canine heart normalized cDNA Library in pBluescript Canis familiaris cDNA clone CH3#002_C07 3', mRNA sequence.    38   0.051  4
BC040936|BC040936.1 Homo sapiens, clone IMAGE:5139212, mRNA, partial cds.    40   0.056  3
CR450713|CR450713.2 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone.    38   0.093  8
DQ030683|DQ030683.1 Homo sapiens HC440 gene, VIRTUAL TRANSCRIPT, partial sequence, genomic survey sequence.    40   0.12   3
CR103667|CR103667.1 Forward strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP142o16.    50   0.13   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54R74) RecName: Full=UPF0681 protein;                               458   e-127
BC040936_1(BC040936|pid:none) Homo sapiens KIAA1033, mRNA (cDNA ...   231   3e-59
AB028956_1(AB028956|pid:none) Homo sapiens mRNA for KIAA1033 pro...   231   4e-59
BC143373_1(BC143373|pid:none) Homo sapiens KIAA1033, mRNA (cDNA ...   231   4e-59
BC020314_1(BC020314|pid:none) Mus musculus RIKEN cDNA A230046K03...   229   1e-58
(Q3UMB9) RecName: Full=UPF0681 protein KIAA1033;                      229   1e-58
AK161247_1(AK161247|pid:none) Mus musculus adult male testis cDN...   229   1e-58
AK145000_1(AK145000|pid:none) Mus musculus mammary gland RCB-052...   229   1e-58
BC110850_1(BC110850|pid:none) Homo sapiens KIAA1033, mRNA (cDNA ...   174   6e-42
CP000600_221(CP000600|pid:none) Ostreococcus lucimarinus CCE9901...   171   7e-41

 psg: 0.71  gvh: 0.25  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.20  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 40.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: plasma membrane

>> prediction for SHB466 is nuc

>SHB466F.Seq
GCCACATCCTATTCGGCTAATAATGGTCAAAATGAAGAATATACATTTGATGCATTTGAA
GATGGTTCATCTAAATTGGTTGGAGAGCAACAATTAACTAAACTTAATCAATTTGCAGTT
GATTATGCACAACAAATTTGGAAGATAGAAGAAGCATTAGATGAAACATTAAATGAAGTA
TGGGATATAAATATAGATCCAGTTTCAATTTATAATAAACCTCATGAACAATTAGATTTA
ATTGAATTGGTTAAAACTGATCATAAAATTTTCAATAAAGTAATATTAGTATTCTCTTCA
ATTTGTAATCAAACTAGAATTTTAAAAGAAACAGCAGAAAGTAAATTTTATTCACCATTA
ACAGTATTTGGAGAGATTACAGGTGAATCATCAGAAGGTGATGTACAAATTGAAGTTGGT
AAATTATTACCATTTATGATTGATTTATCAGCATTTGTAAATAGATGTTATTCATTAATT
AGAAATATTATTAGTCAATTTGCATCGATCTATCAATCACAAAAGAATATACATACACAA
TTCTTTAAGAATGTTCATTTACAAGCTGTCTATTATTCAATGATTGATATATTTTCAGTA
TTAATAAATTTAGATAGTATAATTACACAAAATACTGCTTTANNNNNNNNNN

>SHB466Z.Seq
NNNNNNNNNNGGTGACAGAGTTGGAAAGAGACATTAGACAATTGGGTGTCTCCGAGACTG
GTTTAACCTTTTTAGATCATTTTAGACTTTTAATCACTCATATTGGTAATGCTATGGGTT
ATATTAGATTGGTCAGATCCGGTGGTTTACACTATTGTAGTAATGCTATCAAATTCGTAC
CAGACTTGAAAAAGATTCCAAAATTCCAAGATTTAACCTCAAAAGATGCCCTCTCACCTG
AAACAATTCAAGCTAGTACCAATTTGGATTCAATCATTCATAATCTTTCAAATAATTTAT
CAGAGGGTACTGAATACTTTAAGATGTTGGTAAACGTTTTCGCTACAGAGTTTCGAAATA
TCGCTAATCAACATTTAAAGAATTTCTATATCATCGTACCTGCCTTGGCCTCAAATTACA
TCGATCATATGATCAATAGTAAAGATAAACTCTTTAAGAAATCAAAAGCAATCGGTGCTG
AAGCTTTATTATTCACTGATGATGGCTTTGCAATTGGTTTGGCTTATATCTTAAAATTAT
TAGATCAAAATAAAGATTTCGATTCTCTTCATTGGTTCGATCGTATTCAATTGAAATGTG
AAACNGATCAAAAGAGAATGTTGGCTGAAGCAAATGCAAAAGGTATCAAAGAGGATCAAA
ATCATTTAGCAATTAAAAAGATTCAAAATTTCCAACAAGAATANAATTATTTAAATTCTT
TCTCGGTTCAAGAATTTTCTTAAAG

>SHB466P.Seq
GCCACATCCTATTCGGCTAATAATGGTCAAAATGAAGAATATACATTTGATGCATTTGAA
GATGGTTCATCTAAATTGGTTGGAGAGCAACAATTAACTAAACTTAATCAATTTGCAGTT
GATTATGCACAACAAATTTGGAAGATAGAAGAAGCATTAGATGAAACATTAAATGAAGTA
TGGGATATAAATATAGATCCAGTTTCAATTTATAATAAACCTCATGAACAATTAGATTTA
ATTGAATTGGTTAAAACTGATCATAAAATTTTCAATAAAGTAATATTAGTATTCTCTTCA
ATTTGTAATCAAACTAGAATTTTAAAAGAAACAGCAGAAAGTAAATTTTATTCACCATTA
ACAGTATTTGGAGAGATTACAGGTGAATCATCAGAAGGTGATGTACAAATTGAAGTTGGT
AAATTATTACCATTTATGATTGATTTATCAGCATTTGTAAATAGATGTTATTCATTAATT
AGAAATATTATTAGTCAATTTGCATCGATCTATCAATCACAAAAGAATATACATACACAA
TTCTTTAAGAATGTTCATTTACAAGCTGTCTATTATTCAATGATTGATATATTTTCAGTA
TTAATAAATTTAGATAGTATAATTACACAAAATACTGCTTTA----------GGTGACAG
AGTTGGAAAGAGACATTAGACAATTGGGTGTCTCCGAGACTGGTTTAACCTTTTTAGATC
ATTTTAGACTTTTAATCACTCATATTGGTAATGCTATGGGTTATATTAGATTGGTCAGAT
CCGGTGGTTTACACTATTGTAGTAATGCTATCAAATTCGTACCAGACTTGAAAAAGATTC
CAAAATTCCAAGATTTAACCTCAAAAGATGCCCTCTCACCTGAAACAATTCAAGCTAGTA
CCAATTTGGATTCAATCATTCATAATCTTTCAAATAATTTATCAGAGGGTACTGAATACT
TTAAGATGTTGGTAAACGTTTTCGCTACAGAGTTTCGAAATATCGCTAATCAACATTTAA
AGAATTTCTATATCATCGTACCTGCCTTGGCCTCAAATTACATCGATCATATGATCAATA
GTAAAGATAAACTCTTTAAGAAATCAAAAGCAATCGGTGCTGAAGCTTTATTATTCACTG
ATGATGGCTTTGCAATTGGTTTGGCTTATATCTTAAAATTATTAGATCAAAATAAAGATT
TCGATTCTCTTCATTGGTTCGATCGTATTCAATTGAAATGTGAAACNGATCAAAAGAGAA
TGTTGGCTGAAGCAAATGCAAAAGGTATCAAAGAGGATCAAAATCATTTAGCAATTAAAA
AGATTCAAAATTTCCAACAAGAATANAATTATTTAAATTCTTTCTCGGTTCAAGAATTTT
CTTAAAG