>SHB341 (SHB341Q) /CSM/SH/SHB3-B/SHB341Q.Seq.d/
CTGTTGGCCTACTGGGTAATAAACTTAAAAAACACTACCACTATATTTTCCCACGCTGTA
AAAACAAAAAAACAAAAAACAAAAAAAATAAAAAGAAAAAAAAATCTAAGAATCAAATAC
AAAACTTTAAAAACAATGTCACTCTTCAAGAGAATTACAAATTTGTTTGCTCCAAGTCAA
CAATCCAATTTAATTGAATGTAATGGTTATTTTTATCACTTTTATAAAGGACAATGGGTT
TGTTTATTTAATGATTGTGAATTAGTTATTCATAAATTTGAAAATTATAAATACCAAATT
CAAGTTACAAGAATTGTAGATCAAGAACAAGAAAATGAAAGAAGAAAACAAAGGGAAGCA
GAAGGTTTAGAAGATGAAGAGGAAGAAGAGAATCTTGTAAATTATTTTGAATTTCCAGTT
ACAAATGGATTAGAATTATATAATGAAATTGGACCATTAGGTAATTTCTCATTAAGTTGG
AAAGGTACAAATACAAGATTCTTATTCGAATTTGATAGTAATAATAATAATAACAATAAT
AACAATAATAATAATGAAAGTATTGAAACAACAGGACATCAATTAAAGAGAATATTATGT
CAATGTATTTATGAATCAATGTATTTAACACCATATACAAAXXXXXXXXXXGCTACAATG
GTAATAATATGTTTTTAGTGGATCCACGTGAATCAAAGAATAAAGTTACCGTTAAATTTC
ATGGTGGTTCAAATCCAAAATCTGTAACTACATGTGCTGCCACCACTGGTAGTGGTCAAA
TTGCTTTTGGTACGTCTAAAGGTGAAATCAAACTTTTCAGCAAAACTCAATTCGATCAAA
ATAAACGTTCCGTCACCCCTTCCGACCCTTTGGGTGCTATTGCTCGTTCACGTACTACCC
TACCGGGTATTGGTGATCCAATCGTTGGTATCGATGTCACCAAAGATGGTAAATGGATCG
TTGCAACCTGTAAACAATATATCATGGTCATACCAGCTTCACTCAAAGATGGTAGTAGTG
GTTTTGAAGATCGTTTGGGTGCTCGTCGTCCTTCACCAAAACGTTTAATTTTAAAACCTC
AAGATATTAAACGTATGGGTGGTGTCGTCAATTTCACACCAGCCAAATTCAATATCGTTG
GTGATGAACAATCTGAAACCTCCATTCTCACAAGCACCGGCAGTTTCCTTATCACTTGGA
ATTTTAGAAAAATTAAACAAAACATTTTGGACGTTTATCAAATTAAACAATTCCAAGATG
GTGTTGTAGCTGAACAATTTAAATCAAATAGAGATAATTCAATAGTTGTACTTTCCCGAT
AATGTATTTATCAAAAAAGAAATAAATAACAAAATAATAATAAANATATATAAAA

LLAYWVINLKNTTTIFSHAVKTKKQKTKKIKRKKNLRIKYKTLKTMSLFKRITNLFAPSQ
QSNLIECNGYFYHFYKGQWVCLFNDCELVIHKFENYKYQIQVTRIVDQEQENERRKQREA
EGLEDEEEEENLVNYFEFPVTNGLELYNEIGPLGNFSLSWKGTNTRFLFEFDSNNNNNNN
NNNNNESIETTGHQLKRILCQCIYESMYLTPYT---

---YNGNNMFLVDPRESKNKVTVKFHGGSNPKSVTTCAATTGSGQIAFGTSKGEIKLFSK
TQFDQNKRSVTPSDPLGAIARSRTTLPGIGDPIVGIDVTKDGKWIVATCKQYIMVIPASL
KDGSSGFEDRLGARRPSPKRLILKPQDIKRMGGVVNFTPAKFNIVGDEQSETSILTSTGS
FLITWNFRKIKQNILDVYQIKQFQDGVVAEQFKSNRDNSIVVLSR*ciyqkrnk*qnnnk
xi*

Frame A:
LLAYWVINLKNTTTIFSHAVKTKKQKTKKIKRKKNLRIKYKTLKTMSLFKRITNLFAPSQ
QSNLIECNGYFYHFYKGQWVCLFNDCELVIHKFENYKYQIQVTRIVDQEQENERRKQREA
EGLEDEEEEENLVNYFEFPVTNGLELYNEIGPLGNFSLSWKGTNTRFLFEFDSNNNNNNN
NNNNNESIETTGHQLKRILCQCIYESMYLTPYT---

---atmviicf*wihvnqriklplnfmvvqiqnl*lhvlpplvvvklllvrlkvksnfsa
klnsikinvpsplptlwvlllvhvlpyrvlviqslvsmspkmvngslqpvnniswsyqlh
skmvvvvlkivwvlvvlhqnv*f*nlkilnvwvvssishqpnsislvmnnlkppfsqapa
vslslgileklnktfwtfiklnnskmvl*lnnlnqieiiq*lyfpdnvfikkeinnkiii
xiyk

Frame B:
cwptg**t*ktlplyfptl*kqknkkqkk*kekki*esntkl*kqchssrelqicllqvn
npi*lnvmvifitfikdngfvylmivn*lfinlkiintkfklqel*iknkkmkeenkgkq
kv*kmkrkkril*iilnfqlqmd*nyimkldh*vish*vgkvqiqdsysnliviiiitii
tiiimkvlkqqdin*reyyvnvfmnqci*hhiq---

---lqw**yvfsgst*ike*syr*iswwfkskicnymcchhw*wsncfwyv*r*nqtfqq
nsirsk*tfrhpfrpfgcycsftyyptgyw*snrwyrchqrw*mdrcnl*tiyhghtsft
qrw**wf*rsfgcsssftktfnfktsry*tygwcrqfhtsqiqyrw**ti*nlhshkhrq
fpyhlef*kn*tkhfgrlsn*tiprwccs*ti*ik*r*fnsctfpimylskkk*itk***
xyik

Frame C:
vgllgnklkkhyhyifprcknkktknkknkkkkksknqiqnfknnvtlqenykfvcskst
iqfn*m*wlflsll*rtmglfi**l*isys*i*kl*ipnssykncrsrtrk*kkktkgsr
rfrr*rgrrescklf*issykwirii**nwtir*fliklerykykiliri*******q**
q****ky*nnrtsikenimsmyl*invfntiyk---

---YNGNNMFLVDPRESKNKVTVKFHGGSNPKSVTTCAATTGSGQIAFGTSKGEIKLFSK
TQFDQNKRSVTPSDPLGAIARSRTTLPGIGDPIVGIDVTKDGKWIVATCKQYIMVIPASL
KDGSSGFEDRLGARRPSPKRLILKPQDIKRMGGVVNFTPAKFNIVGDEQSETSILTSTGS
FLITWNFRKIKQNILDVYQIKQFQDGVVAEQFKSNRDNSIVVLSR*ciyqkrnk*qnnnk
xi*

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHB341 (SHB341Q) /CSM/SH/SHB3-B/SHB341Q.Seq.d/                       1481   0.0
SHE773 (SHE773Q) /CSM/SH/SHE7-D/SHE773Q.Seq.d/                       1310   0.0
SHB712 (SHB712Q) /CSM/SH/SHB7-A/SHB712Q.Seq.d/                       1302   0.0
CHM543 (CHM543Q) /CSM/CH/CHM5-B/CHM543Q.Seq.d/                       1302   0.0
AHH419 (AHH419Q) /CSM/AH/AHH4-A/AHH419Q.Seq.d/                       1302   0.0
AHH330 (AHH330Q) /CSM/AH/AHH3-B/AHH330Q.Seq.d/                       1302   0.0
AHA610 (AHA610Q) /CSM/AH/AHA6-A/AHA610Q.Seq.d/                       1302   0.0
AHM787 (AHM787Q) /CSM/AH/AHM7-D/AHM787Q.Seq.d/                       1298   0.0
AHD848 (AHD848Q) /CSM/AH/AHD8-B/AHD848Q.Seq.d/                       1296   0.0
AHN469 (AHN469Q) /CSM/AH/AHN4-C/AHN469Q.Seq.d/                       1289   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.  1296   0.0    1
AC068203|AC068203.2 Homo sapiens chromosome 4 clone RP11-683C4 map 4, WORKING DRAFT SEQUENCE, 18 unordered pieces.    52   0.031  1
AC104814|AC104814.5 Homo sapiens BAC clone RP11-683C4 from 4, complete sequence.    52   0.031  1
AC093790|AC093790.3 Homo sapiens BAC clone RP11-250F14 from 4, complete sequence.    52   0.031  1
CO731046|CO731046.1 JBW004G03.b_019.abi Pineapple green mature fruit cDNA library Ananas comosus cDNA clone JBW004G03 similar to unknown protein, mRNA sequence.    50   0.12   1
CO730778|CO730778.1 JBW001D09.b_073.abi Pineapple green mature fruit cDNA library Ananas comosus cDNA clone JBW001D09 similar to unknown protein, mRNA sequence.    50   0.12   1
AP008101|AP008101.1 Lotus corniculatus var. japonicus genomic DNA, clone:LjT22H07, TM1476.    50   0.12   1
CJ479865|CJ479865.1 Macaca fascicularis mRNA, clone: QtrA-15269, 5' end, expressed in brain temporal lobe.    44   0.16   2
AE014829|AE014829.1 Plasmodium falciparum 3D7 chromosome 10 section 1 of 7 of the complete sequence.    42   0.25   8
AC157501|AC157501.1 Medicago truncatula chromosome 7 clone mte1-27e16, *** SEQUENCING IN PROGRESS ***, 12 unordered pieces.    36   0.64   6

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116986_49(AC116986|pid:none) Dictyostelium discoideum chromoso...   438   e-121
EF612188_1(EF612188|pid:none) Dictyostelium minutum unknown (Dm0...   323   1e-86
AE017345_303(AE017345|pid:none) Cryptococcus neoformans var. neo...   165   4e-39
(Q1MTR3) RecName: Full=Vacuolar import and degradation protein 2...   142   2e-32
FM992693_11(FM992693|pid:none) Candida dubliniensis CD36 chromos...   137   7e-31
CU633457_256(CU633457|pid:none) Podospora anserina genomic DNA c...   135   3e-30
CR382130_222(CR382130|pid:none) Yarrowia lipolytica strain CLIB1...   135   3e-30
AM920435_379(AM920435|pid:none) Penicillium chrysogenum Wisconsi...   132   2e-29
AP007151_1191(AP007151|pid:none) Aspergillus oryzae RIB40 genomi...   130   8e-29
CR382135_411(CR382135|pid:none) Debaryomyces hansenii strain CBS...   124   6e-27

 psg: 0.87  gvh: 0.39  alm: 0.41  top: 0.77  tms: 0.00  mit: 0.54  mip: 0.09
 nuc: 0.04  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 80.0 %: mitochondrial
	 12.0 %: cytoplasmic
	  8.0 %: nuclear

>> prediction for SHB341 is mit

>SHB341F.Seq
CTGTTGGCCTACTGGGTAATAAACTTAAAAAACACTACCACTATATTTTCCCACGCTGTA
AAAACAAAAAAACAAAAAACAAAAAAAATAAAAAGAAAAAAAAATCTAAGAATCAAATAC
AAAACTTTAAAAACAATGTCACTCTTCAAGAGAATTACAAATTTGTTTGCTCCAAGTCAA
CAATCCAATTTAATTGAATGTAATGGTTATTTTTATCACTTTTATAAAGGACAATGGGTT
TGTTTATTTAATGATTGTGAATTAGTTATTCATAAATTTGAAAATTATAAATACCAAATT
CAAGTTACAAGAATTGTAGATCAAGAACAAGAAAATGAAAGAAGAAAACAAAGGGAAGCA
GAAGGTTTAGAAGATGAAGAGGAAGAAGAGAATCTTGTAAATTATTTTGAATTTCCAGTT
ACAAATGGATTAGAATTATATAATGAAATTGGACCATTAGGTAATTTCTCATTAAGTTGG
AAAGGTACAAATACAAGATTCTTATTCGAATTTGATAGTAATAATAATAATAACAATAAT
AACAATAATAATAATGAAAGTATTGAAACAACAGGACATCAATTAAAGAGAATATTATGT
CAATGTATTTATGAATCAATGTATTTAACACCATATACAAANNNNNNNNNN

>SHB341Z.Seq
NNNNNNNNNNGCTACAATGGTAATAATATGTTTTTAGTGGATCCACGTGAATCAAAGAAT
AAAGTTACCGTTAAATTTCATGGTGGTTCAAATCCAAAATCTGTAACTACATGTGCTGCC
ACCACTGGTAGTGGTCAAATTGCTTTTGGTACGTCTAAAGGTGAAATCAAACTTTTCAGC
AAAACTCAATTCGATCAAAATAAACGTTCCGTCACCCCTTCCGACCCTTTGGGTGCTATT
GCTCGTTCACGTACTACCCTACCGGGTATTGGTGATCCAATCGTTGGTATCGATGTCACC
AAAGATGGTAAATGGATCGTTGCAACCTGTAAACAATATATCATGGTCATACCAGCTTCA
CTCAAAGATGGTAGTAGTGGTTTTGAAGATCGTTTGGGTGCTCGTCGTCCTTCACCAAAA
CGTTTAATTTTAAAACCTCAAGATATTAAACGTATGGGTGGTGTCGTCAATTTCACACCA
GCCAAATTCAATATCGTTGGTGATGAACAATCTGAAACCTCCATTCTCACAAGCACCGGC
AGTTTCCTTATCACTTGGAATTTTAGAAAAATTAAACAAAACATTTTGGACGTTTATCAA
ATTAAACAATTCCAAGATGGTGTTGTAGCTGAACAATTTAAATCAAATAGAGATAATTCA
ATAGTTGTACTTTCCCGATAATGTATTTATCAAAAAAGAAATAAATAACAAAATAATAAT
AAANATATATAAAA

>SHB341P.Seq
CTGTTGGCCTACTGGGTAATAAACTTAAAAAACACTACCACTATATTTTCCCACGCTGTA
AAAACAAAAAAACAAAAAACAAAAAAAATAAAAAGAAAAAAAAATCTAAGAATCAAATAC
AAAACTTTAAAAACAATGTCACTCTTCAAGAGAATTACAAATTTGTTTGCTCCAAGTCAA
CAATCCAATTTAATTGAATGTAATGGTTATTTTTATCACTTTTATAAAGGACAATGGGTT
TGTTTATTTAATGATTGTGAATTAGTTATTCATAAATTTGAAAATTATAAATACCAAATT
CAAGTTACAAGAATTGTAGATCAAGAACAAGAAAATGAAAGAAGAAAACAAAGGGAAGCA
GAAGGTTTAGAAGATGAAGAGGAAGAAGAGAATCTTGTAAATTATTTTGAATTTCCAGTT
ACAAATGGATTAGAATTATATAATGAAATTGGACCATTAGGTAATTTCTCATTAAGTTGG
AAAGGTACAAATACAAGATTCTTATTCGAATTTGATAGTAATAATAATAATAACAATAAT
AACAATAATAATAATGAAAGTATTGAAACAACAGGACATCAATTAAAGAGAATATTATGT
CAATGTATTTATGAATCAATGTATTTAACACCATATACAAA----------GCTACAATG
GTAATAATATGTTTTTAGTGGATCCACGTGAATCAAAGAATAAAGTTACCGTTAAATTTC
ATGGTGGTTCAAATCCAAAATCTGTAACTACATGTGCTGCCACCACTGGTAGTGGTCAAA
TTGCTTTTGGTACGTCTAAAGGTGAAATCAAACTTTTCAGCAAAACTCAATTCGATCAAA
ATAAACGTTCCGTCACCCCTTCCGACCCTTTGGGTGCTATTGCTCGTTCACGTACTACCC
TACCGGGTATTGGTGATCCAATCGTTGGTATCGATGTCACCAAAGATGGTAAATGGATCG
TTGCAACCTGTAAACAATATATCATGGTCATACCAGCTTCACTCAAAGATGGTAGTAGTG
GTTTTGAAGATCGTTTGGGTGCTCGTCGTCCTTCACCAAAACGTTTAATTTTAAAACCTC
AAGATATTAAACGTATGGGTGGTGTCGTCAATTTCACACCAGCCAAATTCAATATCGTTG
GTGATGAACAATCTGAAACCTCCATTCTCACAAGCACCGGCAGTTTCCTTATCACTTGGA
ATTTTAGAAAAATTAAACAAAACATTTTGGACGTTTATCAAATTAAACAATTCCAAGATG
GTGTTGTAGCTGAACAATTTAAATCAAATAGAGATAATTCAATAGTTGTACTTTCCCGAT
AATGTATTTATCAAAAAAGAAATAAATAACAAAATAATAATAAANATATATAAAA