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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHB256
Library SH
(Link to library)
Clone ID SHB256
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16361-1
Original site URL
Representative seq. ID SHB256P
(Link to Original site)
Representative DNA sequence 
>SHB256 (SHB256Q) /CSM/SH/SHB2-C/SHB256Q.Seq.d/
CTGTTGGCCTACTGGNANAATACTGTGATGGACTTTGGTGAAGATGATGGTGGTAGTGGT
GACGATGGTAATATAACCGAAGGTTTACCAATTAGTGAAGGTATGGATGATTTACCATCA
TCTTCAAACTCTCGTGGCGGCAGCGGTAATGATGAACAAAAGAAACAGTTCCCAAAGGAA
ATGAATTCTCCATCATCGGAATATGGCACAACAAGTGGCGGTCAAAGATTTGATACATTG
GTTGATCCTGATATTAGTTTAGCAGAGATGGAAGAGAAAATGCGTCAACACAAGGTATAC
CAAGAGCAACAACAGCAACAACAACAACAGCAACAACAACAAAAGCAAAAAGATAAAGAG
TTGTCATCACAAAAGAAGAAACCATCATCAATGCAATTAAGTAAAAAGAAACATGTCGCC
AAAGAAGATTCTGAAACATTAGAGACAATCATTGGTGAAGAGAAGAAGGAGGTTGTATTT
GAAGTTAAACCATACTTTTCTCATGCAATTCTTCAAGCAACCATGGCGGTATTTTTAATT
TGGAATATTTTTTACTTTGCATATCGTGCCGGTTGGACAATGAATAGAACCGATTATATC
ACATTCTCTTXXXXXXXXXXACCAATCGTTGTAAAAGATCCAATCATTTTCATTCTCGTC
GGTATGATTCCTGTAATGGTGTTACCAAGAGTCATTCAATATATGATTCTTAGAGCCAAG
AGACCTTATGAAGCCGGTAAGAGTGGTCCATCACTTTGGGTAGAAGCTACTGATCTTTGG
AGAGCTGAACAAACTTTCTTTGGTTTTGCTGGTACTTATATTTCATCATGGAGAGAAGGT
TCCGCTTCAATCGTTAAACTTCTTAAAACTAGAAAGATTAGTCGTCATAAGTTAGCCATG
TGGAATTGGAAGAGAGATTTCGTAAAGAAACCAGTAGTTTGTGAAGTTTTCCGTCAAACT
AAATTGGTCAATGAAAATGATAATGCTCAAGAATCAAGTGGTAAACATAAGGCTGAACAA
TCTTTCCGTACTTCAAACAAAGAATCTGATACCATTAAAAATTCAAGACTTTTCCTTCCA
AATATTATCCTCTTTGTTGTTAATATCTTGGCTATGATGAGTGCTGTACTTAGATTTAAT
TGTTTCCAAAATGATATGTGGCTTTTGGTTGTCGTAGCTGGTTTCTCTTTCTCTACCTTA
TGGCATTTATGGTCTTTCATTCCAATGGCTCTTCGTCAAAGTNAAAAACAAGGCCATATG
CTTCTTCATACCATGCTCACAATATGTTTATTCT
sequence update 2002.10.25
Translated Amino Acid sequence 
LLAYWXNTVMDFGEDDGGSGDDGNITEGLPISEGMDDLPSSSNSRGGSGNDEQKKQFPKE
MNSPSSEYGTTSGGQRFDTLVDPDISLAEMEEKMRQHKVYQEQQQQQQQQQQQQKQKDKE
LSSQKKKPSSMQLSKKKHVAKEDSETLETIIGEEKKEVVFEVKPYFSHAILQATMAVFLI
WNIFYFAYRAGWTMNRTDYITFS---

---PIVVKDPIIFILVGMIPVMVLPRVIQYMILRAKRPYEAGKSGPSLWVEATDLWRAEQ
TFFGFAGTYISSWREGSASIVKLLKTRKISRHKLAMWNWKRDFVKKPVVCEVFRQTKLVN
ENDNAQESSGKHKAEQSFRTSNKESDTIKNSRLFLPNIILFVVNILAMMSAVLRFNCFQN
DMWLLVVVAGFSFSTLWHLWSFIPMALRQSXKQGHMLLHTMLTICLF
Translated Amino Acid sequence (All Frames) 
Frame A:
LLAYWXNTVMDFGEDDGGSGDDGNITEGLPISEGMDDLPSSSNSRGGSGNDEQKKQFPKE
MNSPSSEYGTTSGGQRFDTLVDPDISLAEMEEKMRQHKVYQEQQQQQQQQQQQQKQKDKE
LSSQKKKPSSMQLSKKKHVAKEDSETLETIIGEEKKEVVFEVKPYFSHAILQATMAVFLI
WNIFYFAYRAGWTMNRTDYITFS---

---tnrckrsnhfhsrrydscngvtkshsiyds*sqetl*sr*ewsitlgrsy*sles*t
nflwfcwylyfimerrfrfnr*ts*n*kd*ss*vshveleerfrketssl*sfpsn*igq
*k**csrikw*t*g*tifpyfkqri*yh*kfktfpskyyplcc*ylgydecct*i*lfpk
*yvafgcrswflflylmafmvfhsngssskxktrpyassyhahnmfi

Frame B:
cwptgxil*wtlvkmmvvvvtmvi*pkvyqlvkvwmiyhhlqtlvaaavmmnkrnssqrk
*ilhhrnmaqqvavkdlihwlililv*qrwkrkcvntrytksnnsnnnnsnnnkskkiks
chhkrrnhhqcn*vkrnmspkkilkh*rqslvkrrrrlylklnhtflmqffkqpwryf*f
gifftlhivpvgq*iepiishsl---

---PIVVKDPIIFILVGMIPVMVLPRVIQYMILRAKRPYEAGKSGPSLWVEATDLWRAEQ
TFFGFAGTYISSWREGSASIVKLLKTRKISRHKLAMWNWKRDFVKKPVVCEVFRQTKLVN
ENDNAQESSGKHKAEQSFRTSNKESDTIKNSRLFLPNIILFVVNILAMMSAVLRFNCFQN
DMWLLVVVAGFSFSTLWHLWSFIPMALRQSXKQGHMLLHTMLTICLF

Frame C:
vgllxxycdglw*r*ww*w*rw*ynrrftn**ryg*ftiifklswrqr***tketvpkgn
efsiigiwhnkwrski*yig*s*y*fsrdgrenastqgiprattattttatttkakr*rv
vitkeetiinaik*ketcrqrrf*nirdnhw*reeggci*s*tilfscnsssnhggifnl
eyfllciscrldne*nrlyhil---

---qsl*kiqsfsfssv*fl*wcyqesfni*fleprdlmkpvrvvhhfg*kllifgelnk
lslvllvlifhhgekvplqslnflklerlvvis*pcgigreis*rnq*fvkfsvklnwsm
kmimlknqvvnirlnnlsvlqtknliplkiqdfsfqilssllliswl**vlyldlivskm
icgfwls*lvslslpygiyglsfqwlfvkvknkaicffipcsqyvys
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHB256 (SHB256Q) /CSM/SH/SHB2-C/SHB256Q.Seq.d/                       1822   0.0
SHB183 (SHB183Q) /CSM/SH/SHB1-D/SHB183Q.Seq.d/                       1322   0.0
SHB158 (SHB158Q) /CSM/SH/SHB1-C/SHB158Q.Seq.d/                       1322   0.0
SHB474 (SHB474Q) /CSM/SH/SHB4-D/SHB474Q.Seq.d/                       1306   0.0
CHG748 (CHG748Q) /CSM/CH/CHG7-B/CHG748Q.Seq.d/                       1306   0.0
AHI416 (AHI416Q) /CSM/AH/AHI4-A/AHI416Q.Seq.d/                       1306   0.0
SHB704 (SHB704Q) /CSM/SH/SHB7-A/SHB704Q.Seq.d/                       1304   0.0
CHO542 (CHO542Q) /CSM/CH/CHO5-B/CHO542Q.Seq.d/                       1304   0.0
AHI433 (AHI433Q) /CSM/AH/AHI4-B/AHI433Q.Seq.d/                       1304   0.0
CHO231 (CHO231Q) /CSM/CH/CHO2-B/CHO231Q.Seq.d/                       1302   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF163835|AF163835.1 Dictyostelium discoideum cellulose synthase (dcsA) mRNA, complete cds.  1237   0.0    2
AC006097|AC006097.1 Homo sapiens chromosome 10 clone CIT987SK-1031G15 map 10q25, *** SEQUENCING IN PROGRESS ***, 1 ordered pieces.    54   0.007  1
AC004480|AC004480.7 Homo sapiens chromosome 4, WORKING DRAFT SEQUENCE, 7 unordered pieces.    54   0.007  1
AC093323|AC093323.3 Homo sapiens BAC clone RP11-539L10 from 4, complete sequence.    54   0.007  1
AL390197|AL390197.19 Human DNA sequence from clone RP11-190F19 on chromosome 10.    54   0.007  1
AC154396|AC154396.11 Mus musculus chromosome 3, clone RP24-254D5, complete sequence.    36   0.26   2
CX098227|CX098227.1 EHAHI57TR E. histolytica Normalized cDNA library Entamoeba histolytica cDNA, mRNA sequence.    48   0.46   1
CX096781|CX096781.1 EHAGX29TR E. histolytica Normalized cDNA library Entamoeba histolytica cDNA, mRNA sequence.    48   0.46   1
CX080440|CX080440.1 EHAA717TR E. histolytica Normalized cDNA library Entamoeba histolytica cDNA, mRNA sequence.    48   0.46   1
CX080439|CX080439.1 EHAA717TF E. histolytica Normalized cDNA library Entamoeba histolytica cDNA, mRNA sequence.    48   0.46   1
dna update 2005.12.21
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q9U720) RecName: Full=Cellulose synthase catalytic subunit A [U...   421   e-118
CP001279_992(CP001279|pid:none) Nautilia profundicola AmH, compl...    40   0.13
CP000852_441(CP000852|pid:none) Caldivirga maquilingensis IC-167...    36   2.5
CP001048_907(CP001048|pid:none) Yersinia pseudotuberculosis PB1/...    35   4.2
CP001025_1222(CP001025|pid:none) Burkholderia ambifaria MC40-6 c...    35   4.2
protein update 2009. 4.15
PSORT 

 psg: 0.75  gvh: 0.40  alm: 0.23  top: 0.57  tms: 0.27  mit: 0.15  mip: 0.00
 nuc: 0.06  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 1.00  m_ : 0.00 

	 32.0 %: plasma membrane
	 24.0 %: endoplasmic reticulum
	 16.0 %: vacuolar
	 12.0 %: nuclear
	  8.0 %: mitochondrial
	  4.0 %: Golgi
	  4.0 %: cytoplasmic

>> prediction for SHB256 is pla
5' end seq. ID SHB256F
5' end seq. 
>SHB256F.Seq
CTGTTGGCCTACTGGNANAATACTGTGATGGACTTTGGTGAAGATGATGGTGGTAGTGGT
GACGATGGTAATATAACCGAAGGTTTACCAATTAGTGAAGGTATGGATGATTTACCATCA
TCTTCAAACTCTCGTGGCGGCAGCGGTAATGATGAACAAAAGAAACAGTTCCCAAAGGAA
ATGAATTCTCCATCATCGGAATATGGCACAACAAGTGGCGGTCAAAGATTTGATACATTG
GTTGATCCTGATATTAGTTTAGCAGAGATGGAAGAGAAAATGCGTCAACACAAGGTATAC
CAAGAGCAACAACAGCAACAACAACAACAGCAACAACAACAAAAGCAAAAAGATAAAGAG
TTGTCATCACAAAAGAAGAAACCATCATCAATGCAATTAAGTAAAAAGAAACATGTCGCC
AAAGAAGATTCTGAAACATTAGAGACAATCATTGGTGAAGAGAAGAAGGAGGTTGTATTT
GAAGTTAAACCATACTTTTCTCATGCAATTCTTCAAGCAACCATGGCGGTATTTTTAATT
TGGAATATTTTTTACTTTGCATATCGTGCCGGTTGGACAATGAATAGAACCGATTATATC
ACATTCTCTTNNNNNNNNNN
Length of 5' end seq. 620
3' end seq. ID SHB256Z
3' end seq. 
>SHB256Z.Seq
NNNNNNNNNNACCAATCGTTGTAAAAGATCCAATCATTTTCATTCTCGTCGGTATGATTC
CTGTAATGGTGTTACCAAGAGTCATTCAATATATGATTCTTAGAGCCAAGAGACCTTATG
AAGCCGGTAAGAGTGGTCCATCACTTTGGGTAGAAGCTACTGATCTTTGGAGAGCTGAAC
AAACTTTCTTTGGTTTTGCTGGTACTTATATTTCATCATGGAGAGAAGGTTCCGCTTCAA
TCGTTAAACTTCTTAAAACTAGAAAGATTAGTCGTCATAAGTTAGCCATGTGGAATTGGA
AGAGAGATTTCGTAAAGAAACCAGTAGTTTGTGAAGTTTTCCGTCAAACTAAATTGGTCA
ATGAAAATGATAATGCTCAAGAATCAAGTGGTAAACATAAGGCTGAACAATCTTTCCGTA
CTTCAAACAAAGAATCTGATACCATTAAAAATTCAAGACTTTTCCTTCCAAATATTATCC
TCTTTGTTGTTAATATCTTGGCTATGATGAGTGCTGTACTTAGATTTAATTGTTTCCAAA
ATGATATGTGGCTTTTGGTTGTCGTAGCTGGTTTCTCTTTCTCTACCTTATGGCATTTAT
GGTCTTTCATTCCAATGGCTCTTCGTCAAAGTNAAAAACAAGGCCATATGCTTCTTCATA
CCATGCTCACAATATGTTTATTCT
Length of 3' end seq. 684
Connected seq. ID SHB256P
Connected seq. 
>SHB256P.Seq
CTGTTGGCCTACTGGNANAATACTGTGATGGACTTTGGTGAAGATGATGGTGGTAGTGGT
GACGATGGTAATATAACCGAAGGTTTACCAATTAGTGAAGGTATGGATGATTTACCATCA
TCTTCAAACTCTCGTGGCGGCAGCGGTAATGATGAACAAAAGAAACAGTTCCCAAAGGAA
ATGAATTCTCCATCATCGGAATATGGCACAACAAGTGGCGGTCAAAGATTTGATACATTG
GTTGATCCTGATATTAGTTTAGCAGAGATGGAAGAGAAAATGCGTCAACACAAGGTATAC
CAAGAGCAACAACAGCAACAACAACAACAGCAACAACAACAAAAGCAAAAAGATAAAGAG
TTGTCATCACAAAAGAAGAAACCATCATCAATGCAATTAAGTAAAAAGAAACATGTCGCC
AAAGAAGATTCTGAAACATTAGAGACAATCATTGGTGAAGAGAAGAAGGAGGTTGTATTT
GAAGTTAAACCATACTTTTCTCATGCAATTCTTCAAGCAACCATGGCGGTATTTTTAATT
TGGAATATTTTTTACTTTGCATATCGTGCCGGTTGGACAATGAATAGAACCGATTATATC
ACATTCTCTT----------ACCAATCGTTGTAAAAGATCCAATCATTTTCATTCTCGTC
GGTATGATTCCTGTAATGGTGTTACCAAGAGTCATTCAATATATGATTCTTAGAGCCAAG
AGACCTTATGAAGCCGGTAAGAGTGGTCCATCACTTTGGGTAGAAGCTACTGATCTTTGG
AGAGCTGAACAAACTTTCTTTGGTTTTGCTGGTACTTATATTTCATCATGGAGAGAAGGT
TCCGCTTCAATCGTTAAACTTCTTAAAACTAGAAAGATTAGTCGTCATAAGTTAGCCATG
TGGAATTGGAAGAGAGATTTCGTAAAGAAACCAGTAGTTTGTGAAGTTTTCCGTCAAACT
AAATTGGTCAATGAAAATGATAATGCTCAAGAATCAAGTGGTAAACATAAGGCTGAACAA
TCTTTCCGTACTTCAAACAAAGAATCTGATACCATTAAAAATTCAAGACTTTTCCTTCCA
AATATTATCCTCTTTGTTGTTAATATCTTGGCTATGATGAGTGCTGTACTTAGATTTAAT
TGTTTCCAAAATGATATGTGGCTTTTGGTTGTCGTAGCTGGTTTCTCTTTCTCTACCTTA
TGGCATTTATGGTCTTTCATTCCAATGGCTCTTCGTCAAAGTNAAAAACAAGGCCATATG
CTTCTTCATACCATGCTCACAATATGTTTATTCT
Length of connected seq. 1284
Full length Seq ID -
Full length Seq. -
Length of full length seq. -