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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHB225
Library SH
(Link to library)
Clone ID SHB225
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11302-1
Original site URL
Representative seq. ID SHB225P
(Link to Original site)
Representative DNA sequence 
>SHB225 (SHB225Q) /CSM/SH/SHB2-B/SHB225Q.Seq.d/
CCTACTGGNAAACTACAAATTTGGNATGTTAAATTAATTATAATTTGTTAATATAACATT
TTGTATATATATATATATAAAAAATAAAAAACTCAAATGACAGGATTTTCAATTCCTAAT
ATGAGTATGTTACTCAAATTAGCAAATAGTAGATTGGTATTACATAAAAATAAAAATTTT
GAACAAAGTAATGTTCATAAAAAAGAGATTGCAGAATTATTACAATTAGGTAAAGAAGAA
CAAGCAAGAGTTAGAACGGTTTCAGTTATTAATGAAGATTATCACACTGAAGTTTTAGGT
ATTTTAATTATTTATTGTGAAACTTTAGCAAATAGAATTAGAGGAATTGAAGGTGTAAAG
GTATGCCCACCAGATTTAAAAGAAGCATGTTGTAGTATTATATTTGCATCACCATATTTA
GATAAACAAGTTGAATTATATAAGATTAGAAAGAGATTAATTGAAAAATTTGGTAAAAAA
TTCCCAGAGGAATGTATAGATTGTTGTTGTATCAATCCAAAGATTGTACATAGATTATCA
AATAAACCACCTGAAGAATCATTGGTAAACTATTATCTTAGTAATATTGCAAAGAAACAT
AATGTAGCXXXXXXXXXXTACCGTATTGGATTGTGGTGATGGTGTTTGTCATACCGTGCC
AATCTATGAAGGTTATTCTATTCCACATGCAATTAAAAGAATCGATATCGGTGGTCGTGA
TATCACAGAGTATTTAATGCGTTTACTCACTGAAAGAGGTTATGCTTTCACTACAACCGC
CGAAAGAGAAATCGTACGTGATATCAAAGAGAAAACTTCATTTGTCTCTCAAGATTTCTA
TGCCTCTATGAATGCTCCAGAGGATAATAATATACTCAAGGATTATACGATGCCCGATAA
ACAAGTTTTAACCATTGGTAAAGAAAGATTCCGTTGTTATGAAGCTTTCTTTGACCCTTC
AGTACTAGGTAAAGATCAAAATGGTATTCATCACCTCTTAAATGAAACTATCACCTCTTG
TGATATTGATATTCGTCGTGATCTCTATAAAAATATTGTAGTTTCTGGTGGTTCAACAAT
GGCAACAGGTTTTGAAAAACGTTTACAAAAAGAAATGGAACTTTTAGTTTCAAATCCTCA
AGTCCAAATTAAAATTAATGCACCACCAAATCGTGATATTAGTGTTTGGTGTGGTGGTTC
GGTTTTAGGNATCTTAAAACTTTTTCGGATCAATGGATTACAAAACAAGAATA
sequence update 2002.10.25
Translated Amino Acid sequence 
ptgklqiwxvkliiic*ynilyiyiykk*KTQMTGFSIPNMSMLLKLANSRLVLHKNKNF
EQSNVHKKEIAELLQLGKEEQARVRTVSVINEDYHTEVLGILIIYCETLANRIRGIEGVK
VCPPDLKEACCSIIFASPYLDKQVELYKIRKRLIEKFGKKFPEECIDCCCINPKIVHRLS
NKPPEESLVNYYLSNIAKKHNV---

---TVLDCGDGVCHTVPIYEGYSIPHAIKRIDIGGRDITEYLMRLLTERGYAFTTTAERE
IVRDIKEKTSFVSQDFYASMNAPEDNNILKDYTMPDKQVLTIGKERFRCYEAFFDPSVLG
KDQNGIHHLLNETITSCDIDIRRDLYKNIVVSGGSTMATGFEKRLQKEMELLVSNPQVQI
KINAPPNRDISVWCGGSVLGILKLFRINGLQNKN
Translated Amino Acid sequence (All Frames) 
Frame A:
ptgklqiwxvkliiic*ynilyiyiykk*KTQMTGFSIPNMSMLLKLANSRLVLHKNKNF
EQSNVHKKEIAELLQLGKEEQARVRTVSVINEDYHTEVLGILIIYCETLANRIRGIEGVK
VCPPDLKEACCSIIFASPYLDKQVELYKIRKRLIEKFGKKFPEECIDCCCINPKIVHRLS
NKPPEESLVNYYLSNIAKKHNV---

---yriglw*wclsyranl*rlfystcn*knryrws*yhrvfnafth*krlcfhynrrkr
nrt*yqrenficlsrflclyecsrg**ytqglydar*tsfnhw*rkipll*sfl*pfstr
*rskwyssplk*nyhll*y*yss*sl*kycsfwwfnngnrf*ktftkrngtfsfkssspn
*n*cttks*y*clvwwfgfrxlktfsdqwitkqe

Frame B:
llxnykfgmln*l*fvnitfciyiyiknkklk*qdfqfli*vcysn*qivdwyyikikil
nkvmfikkrlqnyyn*vkknkqelerfqllmkiitlkf*vf*lfivkl*qieleelkv*r
yahqi*kkhvvvlylhhhi*inklnyirlerd*lknlvknsqrnv*ivvvsiqrlyidyq
inhlknhw*tiilvilqrnim*---

---TVLDCGDGVCHTVPIYEGYSIPHAIKRIDIGGRDITEYLMRLLTERGYAFTTTAERE
IVRDIKEKTSFVSQDFYASMNAPEDNNILKDYTMPDKQVLTIGKERFRCYEAFFDPSVLG
KDQNGIHHLLNETITSCDIDIRRDLYKNIVVSGGSTMATGFEKRLQKEMELLVSNPQVQI
KINAPPNRDISVWCGGSVLGILKLFRINGLQNKN

Frame C:
ywxttnlxc*inynlli*hfvyiyi*kiknsndrifns*yeyvtqisk**igit*k*kf*
tk*cs*krdcriitir*rrtsks*ngfsy**rlsh*sfryfnyll*nfsk*n*rn*rckg
mptrfkrsml*yyicitifr*ts*ii*d*kein*kiw*kiprgmyrlllyqskdct*iik
*tt*riigklls**ycket*cs---

---pywivvmvfvipcqsmkvilfhmqlkesisvvvisqsi*cvyslkevmlslqppkek
syviskrklhlslkismpl*mlqriiiysriircpinkf*plvkkdsvvmklsltlqy*v
kikmvfits*mklsplvilifvvisikil*flvvqqwqqvlknvykkkwnf*fqilkskl
klmhhqivilvfgvvvrf*xs*nffgsmdyktri
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHB225 (SHB225Q) /CSM/SH/SHB2-B/SHB225Q.Seq.d/                       2238   0.0
AHE579 (AHE579Q) /CSM/AH/AHE5-D/AHE579Q.Seq.d/                       2212   0.0
CHN502 (CHN502Q) /CSM/CH/CHN5-A/CHN502Q.Seq.d/                       1253   0.0
VHD820 (VHD820Q) /CSM/VH/VHD8-A/VHD820Q.Seq.d/                       1239   0.0
VHA813 (VHA813Q) /CSM/VH/VHA8-A/VHA813Q.Seq.d/                       1239   0.0
SHE313 (SHE313Q) /CSM/SH/SHE3-A/SHE313Q.Seq.d/                       1239   0.0
SHC311 (SHC311Q) /CSM/SH/SHC3-A/SHC311Q.Seq.d/                       1239   0.0
AHM227 (AHM227Q) /CSM/AH/AHM2-B/AHM227Q.Seq.d/                       1239   0.0
AHL385 (AHL385Q) /CSM/AH/AHL3-D/AHL385Q.Seq.d/                       1239   0.0
AHI741 (AHI741Q) /CSM/AH/AHI7-B/AHI741Q.Seq.d/                       1239   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AY763999|AY763999.1 Marginopora vertebralis clone MvA1250_40 actin type 2 gene, partial cds.    52   1e-16  4
AY763998|AY763998.1 Marginopora vertebralis clone MvA1250_37 actin type 2 gene, partial cds.    52   1e-16  4
AY763997|AY763997.1 Marginopora vertebralis clone MvA1247_25 actin type 2 gene, partial cds.    52   1e-16  4
BW639344|BW639344.1 Dugesia ryukyuensis mRNA, clone: Dr_sW_014_I04, 5' mRNA sequence.    72   1e-15  2
BW635547|BW635547.1 Dugesia ryukyuensis mRNA, clone: Dr_sW_002_L03, 5' mRNA sequence.    72   1e-15  2
BW638759|BW638759.1 Dugesia ryukyuensis mRNA, clone: Dr_sW_012_J20, 5' mRNA sequence.    72   2e-15  2
BW635158|BW635158.1 Dugesia ryukyuensis mRNA, clone: Dr_sW_001_I08, 5' mRNA sequence.    72   2e-15  2
BW636171|BW636171.1 Dugesia ryukyuensis mRNA, clone: Dr_sW_004_J22, 5' mRNA sequence.    72   2e-15  2
BW641221|BW641221.1 Dugesia ryukyuensis mRNA, clone: Dr_sW_020_K08, 5' mRNA sequence.    72   2e-15  2
BW635412|BW635412.1 Dugesia ryukyuensis mRNA, clone: Dr_sW_002_E16, 5' mRNA sequence.    72   2e-15  2
dna update 2005.12.20
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AJ132370_1(AJ132370|pid:none) Allogromia sp. mRNA for actin 1.        254   6e-66
AY452189_1(AY452189|pid:none) Sorosphaera veronicae actin 1 gene...   248   4e-64
AY452190_1(AY452190|pid:none) Sorosphaera veronicae actin 2 gene...   248   4e-64
AY764017_1(AY764017|pid:none) Sorites sp. isolate X-1646 clone S...   246   2e-63
AF363539_1(AF363539|pid:none) Cercomonas sp. ATCC 50319 actin (a...   245   2e-63
DQ980435_1(DQ980435|pid:none) Thaumatomonas sp. TMT002 actin gen...   245   2e-63
AM411663_1(AM411663|pid:none) Plasmodiophora brassicae actII gen...   245   3e-63
AF363536_1(AF363536|pid:none) Cercomonas sp. ATCC 50318 actin-1 ...   245   3e-63
EF455795_1(EF455795|pid:none) Metopus palaeformis clone 28 actin...   245   3e-63
DQ356665_1(DQ356665|pid:none) Thraustochytrium striatum actin ge...   244   4e-63
protein update 2009. 4.15
PSORT 

 psg: 0.87  gvh: 0.48  alm: 0.39  top: 0.70  tms: 0.00  mit: 0.33  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: mitochondrial
	 28.0 %: cytoplasmic
	 28.0 %: nuclear
	  4.0 %: peroxisomal

>> prediction for SHB225 is mit
5' end seq. ID SHB225F
5' end seq. 
>SHB225F.Seq
CCTACTGGNAAACTACAAATTTGGNATGTTAAATTAATTATAATTTGTTAATATAACATT
TTGTATATATATATATATAAAAAATAAAAAACTCAAATGACAGGATTTTCAATTCCTAAT
ATGAGTATGTTACTCAAATTAGCAAATAGTAGATTGGTATTACATAAAAATAAAAATTTT
GAACAAAGTAATGTTCATAAAAAAGAGATTGCAGAATTATTACAATTAGGTAAAGAAGAA
CAAGCAAGAGTTAGAACGGTTTCAGTTATTAATGAAGATTATCACACTGAAGTTTTAGGT
ATTTTAATTATTTATTGTGAAACTTTAGCAAATAGAATTAGAGGAATTGAAGGTGTAAAG
GTATGCCCACCAGATTTAAAAGAAGCATGTTGTAGTATTATATTTGCATCACCATATTTA
GATAAACAAGTTGAATTATATAAGATTAGAAAGAGATTAATTGAAAAATTTGGTAAAAAA
TTCCCAGAGGAATGTATAGATTGTTGTTGTATCAATCCAAAGATTGTACATAGATTATCA
AATAAACCACCTGAAGAATCATTGGTAAACTATTATCTTAGTAATATTGCAAAGAAACAT
AATGTAGCNNNNNNNNNN
Length of 5' end seq. 618
3' end seq. ID SHB225Z
3' end seq. 
>SHB225Z.Seq
NNNNNNNNNNTACCGTATTGGATTGTGGTGATGGTGTTTGTCATACCGTGCCAATCTATG
AAGGTTATTCTATTCCACATGCAATTAAAAGAATCGATATCGGTGGTCGTGATATCACAG
AGTATTTAATGCGTTTACTCACTGAAAGAGGTTATGCTTTCACTACAACCGCCGAAAGAG
AAATCGTACGTGATATCAAAGAGAAAACTTCATTTGTCTCTCAAGATTTCTATGCCTCTA
TGAATGCTCCAGAGGATAATAATATACTCAAGGATTATACGATGCCCGATAAACAAGTTT
TAACCATTGGTAAAGAAAGATTCCGTTGTTATGAAGCTTTCTTTGACCCTTCAGTACTAG
GTAAAGATCAAAATGGTATTCATCACCTCTTAAATGAAACTATCACCTCTTGTGATATTG
ATATTCGTCGTGATCTCTATAAAAATATTGTAGTTTCTGGTGGTTCAACAATGGCAACAG
GTTTTGAAAAACGTTTACAAAAAGAAATGGAACTTTTAGTTTCAAATCCTCAAGTCCAAA
TTAAAATTAATGCACCACCAAATCGTGATATTAGTGTTTGGTGTGGTGGTTCGGTTTTAG
GNATCTTAAAACTTTTTCGGATCAATGGATTACAAAACAAGAATA
Length of 3' end seq. 645
Connected seq. ID SHB225P
Connected seq. 
>SHB225P.Seq
CCTACTGGNAAACTACAAATTTGGNATGTTAAATTAATTATAATTTGTTAATATAACATT
TTGTATATATATATATATAAAAAATAAAAAACTCAAATGACAGGATTTTCAATTCCTAAT
ATGAGTATGTTACTCAAATTAGCAAATAGTAGATTGGTATTACATAAAAATAAAAATTTT
GAACAAAGTAATGTTCATAAAAAAGAGATTGCAGAATTATTACAATTAGGTAAAGAAGAA
CAAGCAAGAGTTAGAACGGTTTCAGTTATTAATGAAGATTATCACACTGAAGTTTTAGGT
ATTTTAATTATTTATTGTGAAACTTTAGCAAATAGAATTAGAGGAATTGAAGGTGTAAAG
GTATGCCCACCAGATTTAAAAGAAGCATGTTGTAGTATTATATTTGCATCACCATATTTA
GATAAACAAGTTGAATTATATAAGATTAGAAAGAGATTAATTGAAAAATTTGGTAAAAAA
TTCCCAGAGGAATGTATAGATTGTTGTTGTATCAATCCAAAGATTGTACATAGATTATCA
AATAAACCACCTGAAGAATCATTGGTAAACTATTATCTTAGTAATATTGCAAAGAAACAT
AATGTAGC----------TACCGTATTGGATTGTGGTGATGGTGTTTGTCATACCGTGCC
AATCTATGAAGGTTATTCTATTCCACATGCAATTAAAAGAATCGATATCGGTGGTCGTGA
TATCACAGAGTATTTAATGCGTTTACTCACTGAAAGAGGTTATGCTTTCACTACAACCGC
CGAAAGAGAAATCGTACGTGATATCAAAGAGAAAACTTCATTTGTCTCTCAAGATTTCTA
TGCCTCTATGAATGCTCCAGAGGATAATAATATACTCAAGGATTATACGATGCCCGATAA
ACAAGTTTTAACCATTGGTAAAGAAAGATTCCGTTGTTATGAAGCTTTCTTTGACCCTTC
AGTACTAGGTAAAGATCAAAATGGTATTCATCACCTCTTAAATGAAACTATCACCTCTTG
TGATATTGATATTCGTCGTGATCTCTATAAAAATATTGTAGTTTCTGGTGGTTCAACAAT
GGCAACAGGTTTTGAAAAACGTTTACAAAAAGAAATGGAACTTTTAGTTTCAAATCCTCA
AGTCCAAATTAAAATTAATGCACCACCAAATCGTGATATTAGTGTTTGGTGTGGTGGTTC
GGTTTTAGGNATCTTAAAACTTTTTCGGATCAATGGATTACAAAACAAGAATA
Length of connected seq. 1243
Full length Seq ID -
Full length Seq. -
Length of full length seq. -