>SHB222 (SHB222Q) /CSM/SH/SHB2-A/SHB222Q.Seq.d/
CTGTTGGCCTACTGGNATCATTGTTAATAGCAAAAGNAAAAATGGCAACAAAAAATACAT
CTCCTTATGAGATTGAATATGCAAAGAGTGATAGATCAACATGTTCAACCTGTCAAAGAG
GTATTAATAAAGAAGCAGTTCGTATTGGTTATAAAACAAAATCAAAACACTTTGATGGAA
TGGATGTATCATGGCATCATTTAAAATGTAAATGTCCACAAGTACCATCATTTACAGATT
TAATTCACTGGGAATACCTTCGTTGGGAAGACCAATTATCAATTAAAACAACTTATTTTT
CATCTGAAAAGTATGATCCAAAATCAGCATCAGAGGTACAAAGAGAGAAATATTTAAAGG
CATTATGGGAAGTTAAAGATAGTATTGCCGATAACTTGAAGGGACCAGCAATTAAAAACA
TCATTCAATATAATAAAGGTTATGTTGATAAGGTATCACCAGTTCATTTACTTCATACTC
TCTCTGATTGGATGTTAAAAGGTCGTCCAGGTAGATGTCCAACTTGTAAGAATTTCGATA
TACTTTTCAATGGTATCGAATATCAATGTAAAGGTTGGATTTCAGGTTTCACAAAATGTG
ATTGGAAXXXXXXXXXXGCCAAGATGCAATTGGTTGAAACTCTTATCGATGTTGATATTG
CCGCTAATCTTAAGAAGCAAACTGAATCACTCGAGGGTAACATGATTGAAAATCAATATA
TTACACTCAAAACCAAACTCACTCCCACTTGATAAAGATAGTGTAGTTTATAAAACCTTG
GTAGATTATGTTGCCAATAGTCAAGATAAACAATTTAGAACCAATATCTGTGTTCAAGAT
ATTCTTTCACTTGAGAGAGAGAATGAAAGTGAACGTTTCACTCCTTGGGCAAAAGATAAG
AATAGATTACTCTTATGGCATGATAGTAGGTTAACTAATTTCGTATCGATTGTCTCGCAG
GGATTAAGAATTGCTCCACCATCTGCTCCAAAAACTGGTTACAGATTTGGTAAAGGTATT
TATTTTGCTGATTGTATTAGTAAATCATTCAGCTATTGTTTCACATCAAGTGATTGTCCA
ACTGCACTTATGTTATTATGTGAAGTTTCACTTGGTGACATGAATGAATTGAAACATGAT
ACCTATATGGAAGAGGCACCACATCCATTCCATAGTACAAAAGCTTTAGGTATGGCTGCT
CCTCACAAAGATGGTAATCATCCTCTTTCTGATTCTGATGGTTTAGTAGTACCATTAGGT
AAAATTTCAAAGACTGGTCTCTCAACTTCATGTACTCACAATGAATTCATTGTCTATAAA
ATNAACAAGTCAGAATCAAATACATCTTAAGAGTT

VGLLXSLLIAKXKMATKNTSPYEIEYAKSDRSTCSTCQRGINKEAVRIGYKTKSKHFDGM
DVSWHHLKCKCPQVPSFTDLIHWEYLRWEDQLSIKTTYFSSEKYDPKSASEVQREKYLKA
LWEVKDSIADNLKGPAIKNIIQYNKGYVDKVSPVHLLHTLSDWMLKGRPGRCPTCKNFDI
LFNGIEYQCKGWISGFTKCDW---

---AKMQLVETLIDVDIAANLKKQTESLEGNMIENQYITLKTKLTPT**r*csl*nlgrl
ccq*sr*ti*nqylcsrysft*ere*k*tfhslgkr*e*itlma***vn*fridclagik
ncsticsknwlqiw*rylfc*ly**iiqllfhik*lsnctyvim*sftw*he*iet*yly
grgttsip*yksfrygcssqrw*sssf*f*wfsstir*nfkdwslnfmysq*ihcl*nxq
vrikyilrv

Frame A:
llaywxhc**qkxkwqqkihllmrlnmqrvidqhvqpvkevlikkqfvlvikqnqntlme
wmyhgii*nvnvhkyhhlqi*ftgntfvgktnyqlkqlifhlksmiqnqhqrykerni*r
hygklkivlpit*rdqqlktsfniikvmliryhqfiyfilsligc*kvvqvdvqlvrisi
yfsmvsninvkvgfqvsqnvig---

---AKMQLVETLIDVDIAANLKKQTESLEGNMIENQYITLKTKLTPT**r*csl*nlgrl
ccq*sr*ti*nqylcsrysft*ere*k*tfhslgkr*e*itlma***vn*fridclagik
ncsticsknwlqiw*rylfc*ly**iiqllfhik*lsnctyvim*sftw*he*iet*yly
grgttsip*yksfrygcssqrw*sssf*f*wfsstir*nfkdwslnfmysq*ihcl*nxq
vrikyilrv

Frame B:
cwptgiivnskxkngnkkyisl*d*icke**inmfnlskry**rsssywl*nkiktl*wn
gcimasfkm*mststiiyrfnslgipslgrpiin*nnlffi*kv*skisirgtkreifkg
imgs*r*ycr*legtsn*khhsi**rlc**gitssftsysl*ldvkrssr*msnl*efry
tfqwyrism*rldfrfhkm*le---

---prcnwlkllsmlilplilrsklnhsrvt*lkinilhskpnslpldkdsvvyktlvdy
vansqdkqfrtnicvqdilslereneserftpwakdknrlllwhdsrltnfvsivsqglr
iappsapktgyrfgkgiyfadcisksfsycftssdcptalmllcevslgdmnelkhdtym
eeaphpfhstkalgmaaphkdgnhplsdsdglvvplgkisktglstscthnefivykxnk
sesnts*e

Frame C:
VGLLXSLLIAKXKMATKNTSPYEIEYAKSDRSTCSTCQRGINKEAVRIGYKTKSKHFDGM
DVSWHHLKCKCPQVPSFTDLIHWEYLRWEDQLSIKTTYFSSEKYDPKSASEVQREKYLKA
LWEVKDSIADNLKGPAIKNIIQYNKGYVDKVSPVHLLHTLSDWMLKGRPGRCPTCKNFDI
LFNGIEYQCKGWISGFTKCDW---

---qdaig*nsyrc*ycr*s*ean*itrg*hd*ksiyytqnqthshlikiv*fikpw*im
lpivkinnlepisvfkiffhlrermkvnvsllgqkiridysygmivg*lisyrlsrrd*e
llhhllqklvtdlvkvfillivlvnhsaivshqvivqlhlcyyvkfhlvt*mn*nmipiw
krhhihsivqkl*vwllltkmviilflilmv**yh*vkfqrlvsqlhvltmnslsikxts
qnqihlks

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHB222 (SHB222Q) /CSM/SH/SHB2-A/SHB222Q.Seq.d/                       2609   0.0
AHB712 (AHB712Q) /CSM/AH/AHB7-A/AHB712Q.Seq.d/                       1435   0.0
AHI282 (AHI282Q) /CSM/AH/AHI2-D/AHI282Q.Seq.d/                       1423   0.0
VHB869 (VHB869Q) /CSM/VH/VHB8-C/VHB869Q.Seq.d/                       1419   0.0
SHG306 (SHG306Q) /CSM/SH/SHG3-A/SHG306Q.Seq.d/                       1403   0.0
VHB303 (VHB303Q) /CSM/VH/VHB3-A/VHB303Q.Seq.d/                       1388   0.0
AHR444 (AHR444Q) /CSM/AH/AHR4-B/AHR444Q.Seq.d/                       1378   0.0
AHM188 (AHM188Q) /CSM/AH/AHM1-D/AHM188Q.Seq.d/                       1352   0.0
AHQ185 (AHQ185Q) /CSM/AH/AHQ1-D/AHQ185Q.Seq.d/                       1342   0.0
SHB426 (SHB426Q) /CSM/SH/SHB4-B/SHB426Q.Seq.d/                       1336   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AJ534948|AJ534948.1 Dictyostelium discoideum mRNA for NAD(+) ADP-ribosyltransferase-1A (adprt-1A gene).  1138   0.0    3
AJ520099|AJ520099.1 Dictyostelium discoideum mRNA for (ADP-ribosyl)transferase (adprt2  gene).    62   4e-13  3
CR356247|CR356247.4 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone.    38   0.42   9
CL958527|CL958527.1 OsIFCC001618 Oryza sativa Express Library Oryza sativa (indica cultivar-group) genomic, genomic survey sequence.    48   0.48   1
AC132117|AC132117.3 Mus musculus BAC clone RP24-162P19 from 6, complete sequence.    38   0.68   5
BX936323|BX936323.5 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone CH211-213J22.    36   0.96   2
CR854911|CR854911.13 Zebrafish DNA sequence from clone DKEY-46O18 in linkage group 9.    36   0.96   2
AC021731|AC021731.3 Homo sapiens chromosome 11 clone RP11-693K15 map 11, WORKING DRAFT SEQUENCE, 13 unordered pieces.    36   0.97   2
AC010943|AC010943.3 Homo sapiens clone RP11-115E19, WORKING DRAFT SEQUENCE, 9 unordered pieces.    36   0.98   2
AP003715|AP003715.3 Homo sapiens genomic DNA, chromosome 11q clone:RP11-115E19.    36   0.98   2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

PDBF(1A26) MOL_ID: 1; MOLECULE: POLY (ADP-RIBOSE) POLYMERASE; CH...   170   1e-40
X65497_1(X65497|pid:none) R.norvegicus mRNA for poly(ADP-ribose)...   170   1e-40
(P26446) RecName: Full=Poly [ADP-ribose] polymerase 1;          ...   170   1e-40
(P27008) RecName: Full=Poly [ADP-ribose] polymerase 1;          ...   170   1e-40
AK303340_1(AK303340|pid:none) Homo sapiens cDNA FLJ53442 complet...   168   3e-40
(Q9R152) RecName: Full=Poly [ADP-ribose] polymerase 1;          ...   168   4e-40
AK312339_1(AK312339|pid:none) Homo sapiens cDNA, FLJ92658, highl...   167   6e-40
J03473_1(J03473|pid:none) Human poly(ADP-ribose) synthetase mRNA...   167   6e-40
AB384394_1(AB384394|pid:none) Synthetic construct DNA, clone: pF...   167   6e-40
M17081_1(M17081|pid:none) Human placental poly(ADP-ribose) polym...   167   6e-40

 psg: 0.60  gvh: 0.39  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.27  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 28.0 %: nuclear
	 16.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for SHB222 is cyt

>SHB222F.Seq
CTGTTGGCCTACTGGNATCATTGTTAATAGCAAAAGNAAAAATGGCAACAAAAAATACAT
CTCCTTATGAGATTGAATATGCAAAGAGTGATAGATCAACATGTTCAACCTGTCAAAGAG
GTATTAATAAAGAAGCAGTTCGTATTGGTTATAAAACAAAATCAAAACACTTTGATGGAA
TGGATGTATCATGGCATCATTTAAAATGTAAATGTCCACAAGTACCATCATTTACAGATT
TAATTCACTGGGAATACCTTCGTTGGGAAGACCAATTATCAATTAAAACAACTTATTTTT
CATCTGAAAAGTATGATCCAAAATCAGCATCAGAGGTACAAAGAGAGAAATATTTAAAGG
CATTATGGGAAGTTAAAGATAGTATTGCCGATAACTTGAAGGGACCAGCAATTAAAAACA
TCATTCAATATAATAAAGGTTATGTTGATAAGGTATCACCAGTTCATTTACTTCATACTC
TCTCTGATTGGATGTTAAAAGGTCGTCCAGGTAGATGTCCAACTTGTAAGAATTTCGATA
TACTTTTCAATGGTATCGAATATCAATGTAAAGGTTGGATTTCAGGTTTCACAAAATGTG
ATTGGAANNNNNNNNNN

>SHB222Z.Seq
NNNNNNNNNNGCCAAGATGCAATTGGTTGAAACTCTTATCGATGTTGATATTGCCGCTAA
TCTTAAGAAGCAAACTGAATCACTCGAGGGTAACATGATTGAAAATCAATATATTACACT
CAAAACCAAACTCACTCCCACTTGATAAAGATAGTGTAGTTTATAAAACCTTGGTAGATT
ATGTTGCCAATAGTCAAGATAAACAATTTAGAACCAATATCTGTGTTCAAGATATTCTTT
CACTTGAGAGAGAGAATGAAAGTGAACGTTTCACTCCTTGGGCAAAAGATAAGAATAGAT
TACTCTTATGGCATGATAGTAGGTTAACTAATTTCGTATCGATTGTCTCGCAGGGATTAA
GAATTGCTCCACCATCTGCTCCAAAAACTGGTTACAGATTTGGTAAAGGTATTTATTTTG
CTGATTGTATTAGTAAATCATTCAGCTATTGTTTCACATCAAGTGATTGTCCAACTGCAC
TTATGTTATTATGTGAAGTTTCACTTGGTGACATGAATGAATTGAAACATGATACCTATA
TGGAAGAGGCACCACATCCATTCCATAGTACAAAAGCTTTAGGTATGGCTGCTCCTCACA
AAGATGGTAATCATCCTCTTTCTGATTCTGATGGTTTAGTAGTACCATTAGGTAAAATTT
CAAAGACTGGTCTCTCAACTTCATGTACTCACAATGAATTCATTGTCTATAAAATNAACA
AGTCAGAATCAAATACATCTTAAGAGTT

>SHB222P.Seq
CTGTTGGCCTACTGGNATCATTGTTAATAGCAAAAGNAAAAATGGCAACAAAAAATACAT
CTCCTTATGAGATTGAATATGCAAAGAGTGATAGATCAACATGTTCAACCTGTCAAAGAG
GTATTAATAAAGAAGCAGTTCGTATTGGTTATAAAACAAAATCAAAACACTTTGATGGAA
TGGATGTATCATGGCATCATTTAAAATGTAAATGTCCACAAGTACCATCATTTACAGATT
TAATTCACTGGGAATACCTTCGTTGGGAAGACCAATTATCAATTAAAACAACTTATTTTT
CATCTGAAAAGTATGATCCAAAATCAGCATCAGAGGTACAAAGAGAGAAATATTTAAAGG
CATTATGGGAAGTTAAAGATAGTATTGCCGATAACTTGAAGGGACCAGCAATTAAAAACA
TCATTCAATATAATAAAGGTTATGTTGATAAGGTATCACCAGTTCATTTACTTCATACTC
TCTCTGATTGGATGTTAAAAGGTCGTCCAGGTAGATGTCCAACTTGTAAGAATTTCGATA
TACTTTTCAATGGTATCGAATATCAATGTAAAGGTTGGATTTCAGGTTTCACAAAATGTG
ATTGGAA----------GCCAAGATGCAATTGGTTGAAACTCTTATCGATGTTGATATTG
CCGCTAATCTTAAGAAGCAAACTGAATCACTCGAGGGTAACATGATTGAAAATCAATATA
TTACACTCAAAACCAAACTCACTCCCACTTGATAAAGATAGTGTAGTTTATAAAACCTTG
GTAGATTATGTTGCCAATAGTCAAGATAAACAATTTAGAACCAATATCTGTGTTCAAGAT
ATTCTTTCACTTGAGAGAGAGAATGAAAGTGAACGTTTCACTCCTTGGGCAAAAGATAAG
AATAGATTACTCTTATGGCATGATAGTAGGTTAACTAATTTCGTATCGATTGTCTCGCAG
GGATTAAGAATTGCTCCACCATCTGCTCCAAAAACTGGTTACAGATTTGGTAAAGGTATT
TATTTTGCTGATTGTATTAGTAAATCATTCAGCTATTGTTTCACATCAAGTGATTGTCCA
ACTGCACTTATGTTATTATGTGAAGTTTCACTTGGTGACATGAATGAATTGAAACATGAT
ACCTATATGGAAGAGGCACCACATCCATTCCATAGTACAAAAGCTTTAGGTATGGCTGCT
CCTCACAAAGATGGTAATCATCCTCTTTCTGATTCTGATGGTTTAGTAGTACCATTAGGT
AAAATTTCAAAGACTGGTCTCTCAACTTCATGTACTCACAATGAATTCATTGTCTATAAA
ATNAACAAGTCAGAATCAAATACATCTTAAGAGTT