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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHB219
Library SH
(Link to library)
Clone ID SHB219
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID SHB219P
(Link to Original site)
Representative DNA sequence 
>SHB219 (SHB219Q) /CSM/SH/SHB2-A/SHB219Q.Seq.d/
CACTGTTGGCCTACTGGNACATTGACCCGCAATTAGTAATTAGTAAAGNAATGAAATTAA
AACATTTAACTATTTTTTTATTTATTTTTATTTATAGGTTTTTATTTGTTAAATCGGATT
GTTATCTAATAAACAATGAAAGGCCGGAGNCAAAAATAACCAAAATCCGATGTGATGATC
AAGTTATTTCCATTCTTTTAAATCCAAATAATAGAACTGATGCAACACTGGAAAAGGACA
CTGATGGAAAGTTTTATTTTGATGTTGTTAATTTTCCATACAAGGATAGACTTTGTGATT
ATACCTATGATATTTATTTTTTACCAGAAATCCCATCCCTTCCATCTGTCCCCACAATAG
GAGGATCCGTTAATTTCACATATAACTATCCTTGTACAAGTGTTTATGGTAGAATAAAAC
CTTTGAATATAGCAAACAAAATTGTAACACCATACAATATCAATTTAGACCAGTTCACCA
TGAGAATACAACCAGGCTGTGGAGATTTAATTATAAATTCAGGCTCAAGAGTAATTTATA
ATGCTTCATATCAAACTGGAGATATACCAAATAAACCAACAATCGATGACCAAGGAAATA
TTACCATTCAAGGAAGTAACCTTTACAACACCCAAATTAAAATTAATTCAAATGATCCCG
TTGAAAACXXXXXXXXXXAAATCAACATTCACACTTATTCTAGTTTAACCTGTAATGTAA
CCAACTATGATGAAATGCTAAAATATAACTACTCCAATACTAAATTCAATATCAGTATCT
CTGTTGATGGTCAATACTTTATTGCTCAAATTTTCCAATTTAAATATGAATCAACAATTA
GTTATAGTGAAAATAAGTCATCAGGATTTCCAAATGAAATGTATATCGGTATTGTTGCAA
TTATAATATTTTTAGCATTAATTTTCTTTGCTATTAAAACACAGGTTGAAAAATATATTG
AAGAGAGAAGAGCCAGAAAAGCATTTAGATCAGTTGATAATCTACGTCTTAAACTTCGTG
AGAAACATGCTGCTGAAATCGCTAAACATTATTCATTTGGTTATGAAAGCGCTCCAAAAC
CAAATAAAAGTTATTTCTATGATTTAGGAAAGAAATTATCTAGACTCCCACTCATTAGAT
GTTGTTTCAAAGAACATACTGATTAAATACTATTTCCAAATTCAAATCCAATAATATCCA
ATTCTAAATTAAACCTAAACCAAATTCACCAATCTATAATCAAATAATCAATTTATATCT
TGTAATTGTTTTTGTTCTTGTATCATCTCATCTTCAATATCTATCTACAATTTTTTTCTT
TACTTCAAATATATTTAAACAAATAAATAATAATAAAAAAAAGCAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
LLAYWXIDPQLVISKXMKLKHLTIFLFIFIYRFLFVKSDCYLINNERPEXKITKIRCDDQ
VISILLNPNNRTDATLEKDTDGKFYFDVVNFPYKDRLCDYTYDIYFLPEIPSLPSVPTIG
GSVNFTYNYPCTSVYGRIKPLNIANKIVTPYNINLDQFTMRIQPGCGDLIINSGSRVIYN
ASYQTGDIPNKPTIDDQGNITIQGSNLYNTQIKINSNDPVEN---

---INIHTYSSLTCNVTNYDEMLKYNYSNTKFNISISVDGQYFIAQIFQFKYESTISYSE
NKSSGFPNEMYIGIVAIIIFLALIFFAIKTQVEKYIEERRARKAFRSVDNLRLKLREKHA
AEIAKHYSFGYESAPKPNKSYFYDLGKKLSRLPLIRCCFKEHTD*ilfpnsnpiisnskl
nlnqihqsiik*siyil*lflflyhlifniylqffsllqiylnk*iiikksk
Translated Amino Acid sequence (All Frames) 
Frame A:
hcwptgtltrn**lvkx*n*ni*lffylflfigfyllnrivi**tmkgrxqk*pksdvmi
klfpff*iqiielmqhwkrtlmesfilmllifhtridfviipmififyqkshpfhlspq*
edplishitilvqvfmve*nl*i*qtkl*hhtisi*tssp*eynqavei*l*iqaqe*fi
mlhikleiyqinqqsmtkeilpfkevtfttpklkliqmiplk---

---kstftlilv*pvm*ptmmkc*nittpilnsisvsllmvntlllkfsnlnmnqqlviv
kishqdfqmkcisvllql*yf*h*fslllkhrlknilkreepekhldqliiyvlnfvrnm
llkslniihlvmkalqnqikvismi*ernyldshsldvvsknilikyyfqiqiq*ypiln
*t*tkftnl*snnqfiscncfcsciisssisiynfflyfkyi*tnk***kka

Frame B:
tvgllxh*paisn**xneiktfnyffiyfyl*vfic*igllsnkq*kagxknnqnpm**s
syfhsfksk**n*cntgkgh*wkvlf*cc*fsiqg*tl*lyl*ylfftrnpipsicphnr
rir*fhi*lslykclw*nktfeyskqncntiqyqfrpvhhenttrlwrfnykfrlksnl*
cfisnwrytk*tnnr*prkyyhsrk*plqhpn*n*fk*sr*k---

---nqhshlf*fnl*cnql**naki*llqy*iqyqylc*wsilycsnfpi*i*inn*l**
k*virisk*nvyryccnynifsinflcy*ntg*kiy*reksqksi*is**sts*ts*etc
c*nr*tlfiwl*krsktk*klfl*frkeii*tpth*mlfqrty*lntiskfksnniqf*i
kpkpnspiynqiinlylvivfvlvsshlqylstiffftsnifkqinnnkkkq

Frame C:
LLAYWXIDPQLVISKXMKLKHLTIFLFIFIYRFLFVKSDCYLINNERPEXKITKIRCDDQ
VISILLNPNNRTDATLEKDTDGKFYFDVVNFPYKDRLCDYTYDIYFLPEIPSLPSVPTIG
GSVNFTYNYPCTSVYGRIKPLNIANKIVTPYNINLDQFTMRIQPGCGDLIINSGSRVIYN
ASYQTGDIPNKPTIDDQGNITIQGSNLYNTQIKINSNDPVEN---

---INIHTYSSLTCNVTNYDEMLKYNYSNTKFNISISVDGQYFIAQIFQFKYESTISYSE
NKSSGFPNEMYIGIVAIIIFLALIFFAIKTQVEKYIEERRARKAFRSVDNLRLKLREKHA
AEIAKHYSFGYESAPKPNKSYFYDLGKKLSRLPLIRCCFKEHTD*ilfpnsnpiisnskl
nlnqihqsiik*siyil*lflflyhlifniylqffsllqiylnk*iiikksk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHB219 (SHB219Q) /CSM/SH/SHB2-A/SHB219Q.Seq.d/                       2365   0.0
AHI368 (AHI368Q) /CSM/AH/AHI3-C/AHI368Q.Seq.d/                       1283   0.0
SHG346 (SHG346Q) /CSM/SH/SHG3-B/SHG346Q.Seq.d/                       1269   0.0
SHE746 (SHE746Q) /CSM/SH/SHE7-B/SHE746Q.Seq.d/                       1269   0.0
AHJ848 (AHJ848Q) /CSM/AH/AHJ8-B/AHJ848Q.Seq.d/                       1269   0.0
SHB122 (SHB122Q) /CSM/SH/SHB1-A/SHB122Q.Seq.d/                       1261   0.0
SHL812 (SHL812Q) /CSM/SH/SHL8-A/SHL812Q.Seq.d/                       1255   0.0
SHL593 (SHL593Q) /CSM/SH/SHL5-D/SHL593Q.Seq.d/                       1255   0.0
SHL342 (SHL342Q) /CSM/SH/SHL3-B/SHL342Q.Seq.d/                       1255   0.0
SHK837 (SHK837Q) /CSM/SH/SHK8-B/SHK837Q.Seq.d/                       1255   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116982|AC116982.2 Dictyostelium discoideum chromosome 2 map 3622643-3879522 strain AX4, complete sequence.   216   1e-88  14
DD010735|DD010735.1 Diagnosis of known genetic Parameters within the MHC.    36   5e-04  16
AX344550|AX344550.1 Sequence 1 from Patent WO0200932.              34   0.005  14
AE017308|AE017308.1 Mycoplasma mobile 163K complete genome.        38   0.011  18
CR356245|CR356245.25 Zebrafish DNA sequence from clone CH211-257I15 in linkage group 8.    36   0.018  8
AX344559|AX344559.1 Sequence 10 from Patent WO0200932.             34   0.027  19
CJ439139|CJ439139.1 Macaca fascicularis mRNA, clone: QccE-16392, 5' end, expressed in cerebellum cortex.    42   0.039  2
AZ687404|AZ687404.1 ENTIV16TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    40   0.093  2
AZ532160|AZ532160.1 ENTCE42TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    40   0.093  2
BH150005|BH150005.1 ENTQN09TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    40   0.093  2
dna update 2005.12.20
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

FJ374971_1(FJ374971|pid:none) Dictyostelium discoideum strain AX...   394   e-108
FJ374968_1(FJ374968|pid:none) Dictyostelium discoideum strain QS...   394   e-108
FJ374973_1(FJ374973|pid:none) Dictyostelium discoideum strain QS...   394   e-108
FJ374970_1(FJ374970|pid:none) Dictyostelium discoideum strain QS...   394   e-108
FJ374903_1(FJ374903|pid:none) Dictyostelium discoideum strain QS...   274   4e-72
FJ374895_1(FJ374895|pid:none) Dictyostelium discoideum strain QS...   274   4e-72
FJ374905_1(FJ374905|pid:none) Dictyostelium discoideum strain QS...   273   8e-72
FJ374919_1(FJ374919|pid:none) Dictyostelium discoideum strain QS...   273   8e-72
FJ374902_1(FJ374902|pid:none) Dictyostelium discoideum strain QS...   272   2e-71
FJ374918_1(FJ374918|pid:none) Dictyostelium discoideum strain QS...   272   2e-71
protein update 2009. 4.15
PSORT 

 psg: 0.75  gvh: 0.47  alm: 0.08  top: 0.37  tms: 0.13  mit: 0.27  mip: 0.06
 nuc: 0.06  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 1.00  m3b: 0.00  m_ : 0.00 

	 36.0 %: endoplasmic reticulum
	 20.0 %: mitochondrial
	 16.0 %: plasma membrane
	  8.0 %: extracellular, including cell wall
	  4.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: nuclear
	  4.0 %: vesicles of secretory system

>> prediction for SHB219 is end
5' end seq. ID SHB219F
5' end seq. 
>SHB219F.Seq
CACTGTTGGCCTACTGGNACATTGACCCGCAATTAGTAATTAGTAAAGNAATGAAATTAA
AACATTTAACTATTTTTTTATTTATTTTTATTTATAGGTTTTTATTTGTTAAATCGGATT
GTTATCTAATAAACAATGAAAGGCCGGAGNCAAAAATAACCAAAATCCGATGTGATGATC
AAGTTATTTCCATTCTTTTAAATCCAAATAATAGAACTGATGCAACACTGGAAAAGGACA
CTGATGGAAAGTTTTATTTTGATGTTGTTAATTTTCCATACAAGGATAGACTTTGTGATT
ATACCTATGATATTTATTTTTTACCAGAAATCCCATCCCTTCCATCTGTCCCCACAATAG
GAGGATCCGTTAATTTCACATATAACTATCCTTGTACAAGTGTTTATGGTAGAATAAAAC
CTTTGAATATAGCAAACAAAATTGTAACACCATACAATATCAATTTAGACCAGTTCACCA
TGAGAATACAACCAGGCTGTGGAGATTTAATTATAAATTCAGGCTCAAGAGTAATTTATA
ATGCTTCATATCAAACTGGAGATATACCAAATAAACCAACAATCGATGACCAAGGAAATA
TTACCATTCAAGGAAGTAACCTTTACAACACCCAAATTAAAATTAATTCAAATGATCCCG
TTGAAAACNNNNNNNNNN
Length of 5' end seq. 678
3' end seq. ID SHB219Z
3' end seq. 
>SHB219Z.Seq
NNNNNNNNNNAAATCAACATTCACACTTATTCTAGTTTAACCTGTAATGTAACCAACTAT
GATGAAATGCTAAAATATAACTACTCCAATACTAAATTCAATATCAGTATCTCTGTTGAT
GGTCAATACTTTATTGCTCAAATTTTCCAATTTAAATATGAATCAACAATTAGTTATAGT
GAAAATAAGTCATCAGGATTTCCAAATGAAATGTATATCGGTATTGTTGCAATTATAATA
TTTTTAGCATTAATTTTCTTTGCTATTAAAACACAGGTTGAAAAATATATTGAAGAGAGA
AGAGCCAGAAAAGCATTTAGATCAGTTGATAATCTACGTCTTAAACTTCGTGAGAAACAT
GCTGCTGAAATCGCTAAACATTATTCATTTGGTTATGAAAGCGCTCCAAAACCAAATAAA
AGTTATTTCTATGATTTAGGAAAGAAATTATCTAGACTCCCACTCATTAGATGTTGTTTC
AAAGAACATACTGATTAAATACTATTTCCAAATTCAAATCCAATAATATCCAATTCTAAA
TTAAACCTAAACCAAATTCACCAATCTATAATCAAATAATCAATTTATATCTTGTAATTG
TTTTTGTTCTTGTATCATCTCATCTTCAATATCTATCTACAATTTTTTTCTTTACTTCAA
ATATATTTAAACAAATAAATAATAATAAAAAAAAGCAAA
Length of 3' end seq. 699
Connected seq. ID SHB219P
Connected seq. 
>SHB219P.Seq
CACTGTTGGCCTACTGGNACATTGACCCGCAATTAGTAATTAGTAAAGNAATGAAATTAA
AACATTTAACTATTTTTTTATTTATTTTTATTTATAGGTTTTTATTTGTTAAATCGGATT
GTTATCTAATAAACAATGAAAGGCCGGAGNCAAAAATAACCAAAATCCGATGTGATGATC
AAGTTATTTCCATTCTTTTAAATCCAAATAATAGAACTGATGCAACACTGGAAAAGGACA
CTGATGGAAAGTTTTATTTTGATGTTGTTAATTTTCCATACAAGGATAGACTTTGTGATT
ATACCTATGATATTTATTTTTTACCAGAAATCCCATCCCTTCCATCTGTCCCCACAATAG
GAGGATCCGTTAATTTCACATATAACTATCCTTGTACAAGTGTTTATGGTAGAATAAAAC
CTTTGAATATAGCAAACAAAATTGTAACACCATACAATATCAATTTAGACCAGTTCACCA
TGAGAATACAACCAGGCTGTGGAGATTTAATTATAAATTCAGGCTCAAGAGTAATTTATA
ATGCTTCATATCAAACTGGAGATATACCAAATAAACCAACAATCGATGACCAAGGAAATA
TTACCATTCAAGGAAGTAACCTTTACAACACCCAAATTAAAATTAATTCAAATGATCCCG
TTGAAAAC----------AAATCAACATTCACACTTATTCTAGTTTAACCTGTAATGTAA
CCAACTATGATGAAATGCTAAAATATAACTACTCCAATACTAAATTCAATATCAGTATCT
CTGTTGATGGTCAATACTTTATTGCTCAAATTTTCCAATTTAAATATGAATCAACAATTA
GTTATAGTGAAAATAAGTCATCAGGATTTCCAAATGAAATGTATATCGGTATTGTTGCAA
TTATAATATTTTTAGCATTAATTTTCTTTGCTATTAAAACACAGGTTGAAAAATATATTG
AAGAGAGAAGAGCCAGAAAAGCATTTAGATCAGTTGATAATCTACGTCTTAAACTTCGTG
AGAAACATGCTGCTGAAATCGCTAAACATTATTCATTTGGTTATGAAAGCGCTCCAAAAC
CAAATAAAAGTTATTTCTATGATTTAGGAAAGAAATTATCTAGACTCCCACTCATTAGAT
GTTGTTTCAAAGAACATACTGATTAAATACTATTTCCAAATTCAAATCCAATAATATCCA
ATTCTAAATTAAACCTAAACCAAATTCACCAATCTATAATCAAATAATCAATTTATATCT
TGTAATTGTTTTTGTTCTTGTATCATCTCATCTTCAATATCTATCTACAATTTTTTTCTT
TACTTCAAATATATTTAAACAAATAAATAATAATAAAAAAAAGCAAA
Length of connected seq. 1357
Full length Seq ID -
Full length Seq. -
Length of full length seq. -