>SHB194 (SHB194Q) /CSM/SH/SHB1-D/SHB194Q.Seq.d/
XXXXXXXXXXGGGTTAGAATTAANCNTGCNNCAAANCCATNTGNTGAANCANNTATTACA
TTATCATCATCAATAGCAGCAGGAACAACANCANCANCCACANCAGCAACAGGAATAACA
NCANCAACAACCACAACAGCAGGTCCAGAGAAAACAATAATATCACCAGTTATAATAAAA
CCAGCAGCAGCAACACCAACAACAACAACACCAACAACAACACCAACAACAACAACATCA
CCAACAACAGCAACAATACCAGCAGTATCGACATCAACAATAAAAACAAGTAGTCCAGAC
AGAACAACACCACTTACATCATCACCACCATTAGCATCAACTAAATCAACAGATGAACTA
ATGAAAAAATTGGACCAATTTACACAAGAAACAACACCATCAATAAGAATAGCAACAACA
ACAACACCAACAACAATAACAACACCAACAACAACAGCGACAACCACAACAATAACAACA
GACAAAACAACACCAGTTACATCATCACCACCAACAGCATCTAACATATCAAGTGAAGAT
TTAATGAAAAAGTGGACCAATTTAT

---VRIXXAXXPXXEXXITLSSSIAAGTTXXXTXATGITXXTTTTAGPEKTIISPVIIKP
AAATPTTTTPTTTPTTTTSPTTATIPAVSTSTIKTSSPDRTTPLTSSPPLASTKSTDELM
KKLDQFTQETTPSIRIATTTTPTTITTPTTTATTTTITTDKTTPVTSSPPTASNISSEDL
MKKWTNL

Frame A:
---g*n*xcxkxxx*xxyyiiiinssrnnxxxhxsnrnnxxnnhnsrsrennnitsynkt
sssntnnnntnnntnnnnitnnsnntssidinnknk*srqnnttyiittisin*inr*tn
ekigpiytrnntinknsnnnntnnnnntnnnsdnhnnnnrqnntsyiittnsi*hik*rf
nekvdqf


Frame B:
---VRIXXAXXPXXEXXITLSSSIAAGTTXXXTXATGITXXTTTTAGPEKTIISPVIIKP
AAATPTTTTPTTTPTTTTSPTTATIPAVSTSTIKTSSPDRTTPLTSSPPLASTKSTDELM
KKLDQFTQETTPSIRIATTTTPTTITTPTTTATTTTITTDKTTPVTSSPPTASNISSEDL
MKKWTNL


Frame C:
---lelxxxqxhxxxxxlhyhhq*qqeqxxxpxqqqe*xxqqpqqqvqrkq*yhql**nq
qqqhqqqqhqqqhqqqqhhqqqqqyqqyrhqq*kqvvqteqhhlhhhhh*hqlnqqmn**
knwtnlhkkqhhq*e*qqqqhqqq*qhqqqqrqpqq*qqtkqhqlhhhhqqhltyqvki*
*ksgpiy

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHE433 (VHE433Q) /CSM/VH/VHE4-B/VHE433Q.Seq.d/                        262   5e-69
SHF789 (SHF789Q) /CSM/SH/SHF7-D/SHF789Q.Seq.d/                        262   5e-69
SHB194 (SHB194Q) /CSM/SH/SHB1-D/SHB194Q.Seq.d/                        262   5e-69
CHK709 (CHK709Q) /CSM/CH/CHK7-A/CHK709Q.Seq.d/                        252   5e-66
AHN116 (AHN116Q) /CSM/AH/AHN1-A/AHN116Q.Seq.d/                        252   5e-66
AHJ420 (AHJ420Q) /CSM/AH/AHJ4-A/AHJ420Q.Seq.d/                        252   5e-66
AHC605 (AHC605Q) /CSM/AH/AHC6-A/AHC605Q.Seq.d/                        252   5e-66
AHA705 (AHA705Q) /CSM/AH/AHA7-A/AHA705Q.Seq.d/                        252   5e-66
AHA575 (AHA575Q) /CSM/AH/AHA5-D/AHA575Q.Seq.d/                        252   5e-66
AHL755 (AHL755Q) /CSM/AH/AHL7-C/AHL755Q.Seq.d/                        246   3e-64

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BG979931|BG979931.1 PM2-CN0099-170101-008-f12 CN0099 Homo sapiens cDNA, mRNA sequence.    38   0.013  2
AC115592|AC115592.2 Dictyostelium discoideum chromosome 2 map 1-125959 strain AX4, complete sequence.    34   0.019  5
AC137224|AC137224.1 Rattus norvegicus clone CH230-unknown, *** SEQUENCING IN PROGRESS ***, 4 unordered pieces.    50   0.050  1
AC134015|AC134015.2 Rattus norvegicus clone CH230-524G18, *** SEQUENCING IN PROGRESS ***, 6 unordered pieces.    50   0.050  1
AC106371|AC106371.4 Rattus norvegicus clone CH230-118G5, *** SEQUENCING IN PROGRESS ***, 5 unordered pieces.    50   0.050  1
AC099432|AC099432.5 Rattus norvegicus clone CH230-103G20, *** SEQUENCING IN PROGRESS ***, 9 unordered pieces.    50   0.050  1
BH336507|BH336507.1 CH230-169E2.TJ CHORI-230 Segment 1 Rattus norvegicus genomic clone CH230-169E2, DNA sequence.    38   0.063  3
BZ164233|BZ164233.1 CH230-246O13.TJB CHORI-230 Segment 2 Rattus norvegicus genomic clone CH230-246O13, DNA sequence.    34   0.11   3
AC150458|AC150458.2 Callithrix jacchus clone CH259-31M19, WORKING DRAFT SEQUENCE, 3 ordered pieces.    48   0.20   1
AE003616|AE003616.2 Drosophila melanogaster chromosome 2L, section 25 of 83 of the complete sequence.    46   0.43   3

 ***** No hits found ******

Lambda     K      H
   0.306    0.122    0.324

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3101297
Number of Hits to DB: 263,241,255

 psg: 0.80  gvh: 0.57  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.33  mip: 0.16
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: mitochondrial
	 28.0 %: nuclear
	 16.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: endoplasmic reticulum

>> prediction for SHB194 is mit

>SHB194Z.Seq
NNNNNNNNNNGGGTTAGAATTAANCNTGCNNCAAANCCATNTGNTGAANCANNTATTACA
TTATCATCATCAATAGCAGCAGGAACAACANCANCANCCACANCAGCAACAGGAATAACA
NCANCAACAACCACAACAGCAGGTCCAGAGAAAACAATAATATCACCAGTTATAATAAAA
CCAGCAGCAGCAACACCAACAACAACAACACCAACAACAACACCAACAACAACAACATCA
CCAACAACAGCAACAATACCAGCAGTATCGACATCAACAATAAAAACAAGTAGTCCAGAC
AGAACAACACCACTTACATCATCACCACCATTAGCATCAACTAAATCAACAGATGAACTA
ATGAAAAAATTGGACCAATTTACACAAGAAACAACACCATCAATAAGAATAGCAACAACA
ACAACACCAACAACAATAACAACACCAACAACAACAGCGACAACCACAACAATAACAACA
GACAAAACAACACCAGTTACATCATCACCACCAACAGCATCTAACATATCAAGTGAAGAT
TTAATGAAAAAGTGGACCAATTTAT