>SHB171 (SHB171Q) /CSM/SH/SHB1-C/SHB171Q.Seq.d/
CCTACTAACATTACGTATTCATTTAAACACTGCAGNATAGNAGNATTGGATTCAATCATT
TTTAGATAACGATGGTGTTCAACCAATTTTAAATATTTTAAAGAGACTCGAAAGAAATAA
AAACCGAAAAAGAAGAGCATTCAATTTTACAATGGGAATGTACAAGATGTATTGCAGCAT
TAATGAAAATTAAAATTGGTATGGAATATATTGCATCATTTCCTCAAACTACAAATCTTA
TGGTATTATGTTTGGATACCCCATTAATTAAAGCTAAAACTTTGGTTTTAGAACTTTTAG
CAGCAATTGCAGTTACTGATAAAGGTCATGGCGCCGTTTTAACTTCAATGATTTATCATA
AAGAAGTTAAAAAAGAAATAACTAGATATTTTAATTTAGTACAATCATTAAAAATAGAGA
AAAATGCTGAATATTTAACAACTTGTATGTCATTTATCAATTGTATTATTTCATCACCAA
GTGATTTACCATCACGTATTGAAATTAGAAAAGCATTTTTAAATTTAAAGATTTTAAAAT
ATATTGAAAATTTAAGAGCAGATTATAATGAAGATAAAAATTTATTAACACAATTAGATG
TGTTTGAAGAAGAGTTATCAACAGATGAACAATTAAATTCACAACAAGGTACXXXXXXXX
XXGGTTTCAAGGTGTTGGAAACTTTACCAAAGATGAGAGATGCTCGTAGTAACGATAACA
AACTTTCACTCTTACATTTCTTGGCTAAAACTCTTCAAGATCGTATTCCAGAGATTTGGA
ATATTGGTGCAGAGTTACCACATATCGAACATGCTTCAGAGGTAAGTTTAAATAATATCA
TCAGTGATAGTAGTGAAATTAAACGTTCCATCGATTTGATTGAACGTGATTTCGTACCAA
TGATCAATGATCCATTGTTTGCTCATGATAAACATTGGATTCACAAAATCACCGAGTTCC
AAAAGATCGCCAAAGTACAATATCAAAGAATTGAAAAGGAAATCGATGAAATGAATAAAG
CTTTCGAAGAAATCACCTCTTACTTTGGTGAACCAAAATCAACTCAACCCGATGTTTTCT
TTTCGACAATTAATAACTTTTTAGAAGACCTTGAAAAAGCCTACGGTGAATATCAAGCTA
TGATTAGAAAGGCTGAATTGGAAAATTCAAAAATGGAAGATCCAGAAAAAGGTGGTCTTC
AAGATCTTTCCTCTCAAATTAGATCTGGTCAATTATTCAAAGATAGAAGAGTTGGTGATT
CTGTCATTGCTCAAATGCAAAATGTTGATAGTTTAAGAAAAAATTTAAAATCAACTTCAA
CCACAACTCCAAATACTCCACCAACTATTAAAATNAATACCTTCTCAATCAATTTNAAAC
CATCAGGCCAATAAAAAAATAAT

y*hyvfi*tlqxxxigfnhf*itmvfnqf*if*RDSKEIKTEKEEHSILQWECTRCIAAL
MKIKIGMEYIASFPQTTNLMVLCLDTPLIKAKTLVLELLAAIAVTDKGHGAVLTSMIYHK
EVKKEITRYFNLVQSLKIEKNAEYLTTCMSFINCIISSPSDLPSRIEIRKAFLNLKILKY
IENLRADYNEDKNLLTQLDVFEEELSTDEQLNSQQG---

---GFKVLETLPKMRDARSNDNKLSLLHFLAKTLQDRIPEIWNIGAELPHIEHASEVSLN
NIISDSSEIKRSIDLIERDFVPMINDPLFAHDKHWIHKITEFQKIAKVQYQRIEKEIDEM
NKAFEEITSYFGEPKSTQPDVFFSTINNFLEDLEKAYGEYQAMIRKAELENSKMEDPEKG
GLQDLSSQIRSGQLFKDRRVGDSVIAQMQNVDSLRKNLKSTSTTTPNTPPTIKXNTFSIN
XKPSGQ*knn

Frame A:
ptnitysfkhcxixxldsiifr*rwcstnfkyfketrkk*kpkkksiqfyngnvqdvlqh
**klklvwnilhhflklqilwyyvwiph*lklklwf*nf*qqlqllikvmapf*lq*fii
kklkkk*ldili*ynh*k*rkmlni*qlvchlsivlfhhqviyhhvlklekhf*i*rf*n
ilki*eqiimkikiy*hn*mclkksyqqmnn*ihnkv---

---GFKVLETLPKMRDARSNDNKLSLLHFLAKTLQDRIPEIWNIGAELPHIEHASEVSLN
NIISDSSEIKRSIDLIERDFVPMINDPLFAHDKHWIHKITEFQKIAKVQYQRIEKEIDEM
NKAFEEITSYFGEPKSTQPDVFFSTINNFLEDLEKAYGEYQAMIRKAELENSKMEDPEKG
GLQDLSSQIRSGQLFKDRRVGDSVIAQMQNVDSLRKNLKSTSTTTPNTPPTIKXNTFSIN
XKPSGQ*knn

Frame B:
lltlrihlntax*xxwiqsfldndgvqpilnilkrlernknrkrrafnftmgmykmycsi
nen*nwygiyciissnyksygimfgypin*s*nfgfrtfssncsy**rswrrfnfndls*
rs*krnn*if*fstiiknrekc*ifnnlyviyqlyyfitk*ftity*n*ksifkfkdfki
y*kfksrl**r*kfintircv*rrvinr*tikfttry---

---vsrcwklyqr*emlvvtitnfhsyiswlklfkivfqrfgilvqsyhisnmlqr*v*i
issvivvklnvpsi*lnvisyq*smihcllminigftkspsskrspkynikelkrksmk*
iklskkspltlvnqnqlnpmfsfrqlitf*ktlkkptvnikl*lerlnwkiqkwkiqkkv
vfkifplkldlvnyskieelvilsllkckmliv*eki*nqlqpqlqilhqllkxipsqsi
xnhqankki

Frame C:
y*hyvfi*tlqxxxigfnhf*itmvfnqf*if*RDSKEIKTEKEEHSILQWECTRCIAAL
MKIKIGMEYIASFPQTTNLMVLCLDTPLIKAKTLVLELLAAIAVTDKGHGAVLTSMIYHK
EVKKEITRYFNLVQSLKIEKNAEYLTTCMSFINCIISSPSDLPSRIEIRKAFLNLKILKY
IENLRADYNEDKNLLTQLDVFEEELSTDEQLNSQQG---

---fqgvgnftkdercs**r*qtftltflg*nssrsysrdleywcrvttyrtcfrgkfk*
yhq****n*tfhrfd*t*frtndq*sivcs**tldsqnhrvpkdrqstiskn*kgnr*ne
*sfrrnhlllw*tkinstrcflfdn**lfrrp*kslr*issyd*kg*igkfkngrsrkrw
ssrsflsn*iwsiiqr*ksw*fchcsnakc**fkkkfkinfnhnskystny*nxyllnqf
xtirpikk*

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHB171 (SHB171Q) /CSM/SH/SHB1-C/SHB171Q.Seq.d/                       2670   0.0
AHI806 (AHI806Q) /CSM/AH/AHI8-A/AHI806Q.Seq.d/                       1435   0.0
AHI721 (AHI721Q) /CSM/AH/AHI7-A/AHI721Q.Seq.d/                       1435   0.0
VHD279 (VHD279Q) /CSM/VH/VHD2-D/VHD279Q.Seq.d/                       1396   0.0
AHN833 (AHN833Q) /CSM/AH/AHN8-B/AHN833Q.Seq.d/                       1396   0.0
AFK767 (AFK767Q) /CSM/AF/AFK7-C/AFK767Q.Seq.d/                       1396   0.0
SHK209 (SHK209Q) /CSM/SH/SHK2-A/SHK209Q.Seq.d/                       1372   0.0
SHA320 (SHA320Q) /CSM/SH/SHA3-A/SHA320Q.Seq.d/                       1316   0.0
CHL483 (CHL483Q) /CSM/CH/CHL4-D/CHL483Q.Seq.d/                       1273   0.0
VHN829 (VHN829Q) /CSM/VH/VHN8-B/VHN829Q.Seq.d/                       1243   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AJ748258|AJ748258.1 Dictyostelium discoideum mRNA for diaphanous-related formin dDia2  (drf2 gene).  1372   0.0    3
AE009951|AE009951.2 Fusobacterium nucleatum subsp. nucleatum ATCC 25586, complete genome.    38   6e-05  20
BX842644|BX842644.1 Mycoplasma mycoides subsp. mycoides SC genomic DNA, complete  sequence; segment 3/4.    44   7e-04  16
Z29068|Z29068.2 M.hominis gene for membrane protein P80 (ORF1), membrane lipoprotein protein P60 (ORF2) and putative HIT-like protein (ORF3).    44   0.009  4
AC106872|AC106872.5 Homo sapiens BAC clone RP11-366M4 from 4, complete sequence.    36   0.020  2
BH162258|BH162258.1 ENTRJ10TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    40   0.046  2
BX842643|BX842643.1 Mycoplasma mycoides subsp. mycoides SC genomic DNA, complete  sequence; segment 2/4.    34   0.082  12
AZ550422|AZ550422.1 ENTDT35TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    32   0.16   4
CP000111|CP000111.1 Prochlorococcus marinus str. MIT 9312, complete genome.    32   0.31   26
AC004710|AC004710.3 Plasmodium falciparum chromosome 12, WORKING DRAFT SEQUENCE, 2 unordered pieces.    42   0.34   8

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54N00) RecName: Full=Formin-H; AltName: Full=Diaphanous-relate...   422   e-116
(Q5TJ55) RecName: Full=Formin-D; AltName: Full=Diaphanous-relate...   103   1e-20
(Q0GNC1) RecName: Full=Inverted formin-2;  &DQ834374_1(DQ834374|...    97   1e-18
BC084125_1(BC084125|pid:none) Xenopus laevis hypothetical LOC495...    96   2e-18
BX248757_1(BX248757|pid:none) human full-length cDNA 5-PRIME end...    95   5e-18
(Q27J81) RecName: Full=Inverted formin-2; AltName: Full=HBEBP2-b...    95   5e-18
(Q6NTV6) RecName: Full=Inverted formin-2;  &BC068848_1(BC068848|...    94   1e-17
(Q54WH2) RecName: Full=Formin-A;                                       93   2e-17
(Q6ZPF4) RecName: Full=Formin-like protein 3;  &AK155331_1(AK155...    93   2e-17
AK170744_1(AK170744|pid:none) Mus musculus NOD-derived CD11c +ve...    93   2e-17

 psg: 0.60  gvh: 0.64  alm: 0.31  top: 0.43  tms: 0.07  mit: 0.28  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 20.0 %: cytoplasmic
	 20.0 %: nuclear
	 16.0 %: mitochondrial
	 12.0 %: Golgi
	 12.0 %: endoplasmic reticulum
	  8.0 %: vacuolar
	  4.0 %: peroxisomal
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for SHB171 is cyt

>SHB171F.Seq
CCTACTAACATTACGTATTCATTTAAACACTGCAGNATAGNAGNATTGGATTCAATCATT
TTTAGATAACGATGGTGTTCAACCAATTTTAAATATTTTAAAGAGACTCGAAAGAAATAA
AAACCGAAAAAGAAGAGCATTCAATTTTACAATGGGAATGTACAAGATGTATTGCAGCAT
TAATGAAAATTAAAATTGGTATGGAATATATTGCATCATTTCCTCAAACTACAAATCTTA
TGGTATTATGTTTGGATACCCCATTAATTAAAGCTAAAACTTTGGTTTTAGAACTTTTAG
CAGCAATTGCAGTTACTGATAAAGGTCATGGCGCCGTTTTAACTTCAATGATTTATCATA
AAGAAGTTAAAAAAGAAATAACTAGATATTTTAATTTAGTACAATCATTAAAAATAGAGA
AAAATGCTGAATATTTAACAACTTGTATGTCATTTATCAATTGTATTATTTCATCACCAA
GTGATTTACCATCACGTATTGAAATTAGAAAAGCATTTTTAAATTTAAAGATTTTAAAAT
ATATTGAAAATTTAAGAGCAGATTATAATGAAGATAAAAATTTATTAACACAATTAGATG
TGTTTGAAGAAGAGTTATCAACAGATGAACAATTAAATTCACAACAAGGTACNNNNNNNN
NN

>SHB171Z.Seq
NNNNNNNNNNGGTTTCAAGGTGTTGGAAACTTTACCAAAGATGAGAGATGCTCGTAGTAA
CGATAACAAACTTTCACTCTTACATTTCTTGGCTAAAACTCTTCAAGATCGTATTCCAGA
GATTTGGAATATTGGTGCAGAGTTACCACATATCGAACATGCTTCAGAGGTAAGTTTAAA
TAATATCATCAGTGATAGTAGTGAAATTAAACGTTCCATCGATTTGATTGAACGTGATTT
CGTACCAATGATCAATGATCCATTGTTTGCTCATGATAAACATTGGATTCACAAAATCAC
CGAGTTCCAAAAGATCGCCAAAGTACAATATCAAAGAATTGAAAAGGAAATCGATGAAAT
GAATAAAGCTTTCGAAGAAATCACCTCTTACTTTGGTGAACCAAAATCAACTCAACCCGA
TGTTTTCTTTTCGACAATTAATAACTTTTTAGAAGACCTTGAAAAAGCCTACGGTGAATA
TCAAGCTATGATTAGAAAGGCTGAATTGGAAAATTCAAAAATGGAAGATCCAGAAAAAGG
TGGTCTTCAAGATCTTTCCTCTCAAATTAGATCTGGTCAATTATTCAAAGATAGAAGAGT
TGGTGATTCTGTCATTGCTCAAATGCAAAATGTTGATAGTTTAAGAAAAAATTTAAAATC
AACTTCAACCACAACTCCAAATACTCCACCAACTATTAAAATNAATACCTTCTCAATCAA
TTTNAAACCATCAGGCCAATAAAAAAATAAT

>SHB171P.Seq
CCTACTAACATTACGTATTCATTTAAACACTGCAGNATAGNAGNATTGGATTCAATCATT
TTTAGATAACGATGGTGTTCAACCAATTTTAAATATTTTAAAGAGACTCGAAAGAAATAA
AAACCGAAAAAGAAGAGCATTCAATTTTACAATGGGAATGTACAAGATGTATTGCAGCAT
TAATGAAAATTAAAATTGGTATGGAATATATTGCATCATTTCCTCAAACTACAAATCTTA
TGGTATTATGTTTGGATACCCCATTAATTAAAGCTAAAACTTTGGTTTTAGAACTTTTAG
CAGCAATTGCAGTTACTGATAAAGGTCATGGCGCCGTTTTAACTTCAATGATTTATCATA
AAGAAGTTAAAAAAGAAATAACTAGATATTTTAATTTAGTACAATCATTAAAAATAGAGA
AAAATGCTGAATATTTAACAACTTGTATGTCATTTATCAATTGTATTATTTCATCACCAA
GTGATTTACCATCACGTATTGAAATTAGAAAAGCATTTTTAAATTTAAAGATTTTAAAAT
ATATTGAAAATTTAAGAGCAGATTATAATGAAGATAAAAATTTATTAACACAATTAGATG
TGTTTGAAGAAGAGTTATCAACAGATGAACAATTAAATTCACAACAAGGTAC--------
--GGTTTCAAGGTGTTGGAAACTTTACCAAAGATGAGAGATGCTCGTAGTAACGATAACA
AACTTTCACTCTTACATTTCTTGGCTAAAACTCTTCAAGATCGTATTCCAGAGATTTGGA
ATATTGGTGCAGAGTTACCACATATCGAACATGCTTCAGAGGTAAGTTTAAATAATATCA
TCAGTGATAGTAGTGAAATTAAACGTTCCATCGATTTGATTGAACGTGATTTCGTACCAA
TGATCAATGATCCATTGTTTGCTCATGATAAACATTGGATTCACAAAATCACCGAGTTCC
AAAAGATCGCCAAAGTACAATATCAAAGAATTGAAAAGGAAATCGATGAAATGAATAAAG
CTTTCGAAGAAATCACCTCTTACTTTGGTGAACCAAAATCAACTCAACCCGATGTTTTCT
TTTCGACAATTAATAACTTTTTAGAAGACCTTGAAAAAGCCTACGGTGAATATCAAGCTA
TGATTAGAAAGGCTGAATTGGAAAATTCAAAAATGGAAGATCCAGAAAAAGGTGGTCTTC
AAGATCTTTCCTCTCAAATTAGATCTGGTCAATTATTCAAAGATAGAAGAGTTGGTGATT
CTGTCATTGCTCAAATGCAAAATGTTGATAGTTTAAGAAAAAATTTAAAATCAACTTCAA
CCACAACTCCAAATACTCCACCAACTATTAAAATNAATACCTTCTCAATCAATTTNAAAC
CATCAGGCCAATAAAAAAATAAT