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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHB165
Library SH
(Link to library)
Clone ID SHB165
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16428-1
Original site URL
Representative seq. ID SHB165P
(Link to Original site)
Representative DNA sequence 
>SHB165 (SHB165Q) /CSM/SH/SHB1-C/SHB165Q.Seq.d/
ACTGTTGGCCTACTGGNGNATAGAGTGATAATCAATTATATCATAGNAAATATAATTTTT
ATTTATTTTAATAATATCAAATTAAGCATAACAACTTTTTATAATATTATTATTTAAATT
ATTTCCTCATCAATCCCATCACATAATAAATTAAAATTACAAGAGAAAAAAAAGAAGAAA
CAGCACTTTTTTTTAGCACACCAAACCACACACACACACACATTATATACTATTAAATAA
TGTCACGTTTTTATAGACANGGTTCATCATCAGAATCATCATCAGAGTCATCATCAGATT
CAGATGTTCAAGTAAAAAAAACCAACTCGTTATGTTTCATCATCTGAAGATGAAATTGAA
GAGAAAAGAATCGTTTTATCAGCTAAAGATAAAATTTGGCAACAATTTGATGAAAGTTTA
AAAAAAGTTAGAAATGCTCTTAAAACAAATGATTGGGTTTCAACTACTTCAGAATTTGAT
AATATGACAAAATTAATTACAAATGGTAAAACAACTAGAATTATTGAAAAAGANGGTTTC
CCACCATCATTTATTAAAGCATTATTTATTATTCAAACAAGTCATAGAGATTTAAXXXXX
XXXXXGTCCCTCATCCCAGACATTGACAGTGTTCGTGAAAAACTCACTCGTATCGTTCAA
GAGGTCTCCTTAAAGACTTTCCTCTTCACTTATTCCACTTCTTACGATAGTATCCTCCTC
AGTGAATTGGCCGATCGTTTCCATTTACCAAAGAGTCAAGTTCACTCTATCGTCGCCAAA
ATGATGAATAATCACGAAATCTCTGCCTCAATGGAACACTCTACCGAATCAATCACTTTC
CGTGCTGAACAAACTAAACTCCAATATCTTGCTCTTCATTACTCTGAATCTTTAGTTGAT
TTTGTCGAACAAAATGAACGTATTTACGATGTTAAATTTGGTACCTCCTACAGAAAGAAG
GGTGATAATGATCACTTACCAATCGCTGGTGGTCAACATCATGGTCACCAACATCATGGT
CATCAACACCACGGCCATCATCACCACAATCAACAACAACAACAACATCATCAACAACAA
CAACAAACCACTCAACATCATCACCATCACCACCAAAATCAAAATCAAGGTAACCAACAA
TATCAAAATAAAAAACATCACAATAGCAATCAACAAAAATCTCACANAAAGAG
sequence update 2002.10.25
Translated Amino Acid sequence 
tvgllxxrviinyiixniifiyfnniklsittfyniii*iisssipshnklklqekkkkk
qhfflahqtththtlytik*chvfidxvhhqnhhqshhqiqmfk*KKPTRYVSSSEDEIE
EKRIVLSAKDKIWQQFDESLKKVRNALKTNDWVSTTSEFDNMTKLITNGKTTRIIEKXGF
PPSFIKALFIIQTSHRDL---

---SLIPDIDSVREKLTRIVQEVSLKTFLFTYSTSYDSILLSELADRFHLPKSQVHSIVA
KMMNNHEISASMEHSTESITFRAEQTKLQYLALHYSESLVDFVEQNERIYDVKFGTSYRK
KGDNDHLPIAGGQHHGHQHHGHQHHGHHHHNQQQQQHHQQQQQTTQHHHHHHQNQNQGNQ
QYQNKKHHNSNQQKSHXK
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllxxrviinyiixniifiyfnniklsittfyniii*iisssipshnklklqekkkkk
qhfflahqtththtlytik*chvfidxvhhqnhhqshhqiqmfk*KKPTRYVSSSEDEIE
EKRIVLSAKDKIWQQFDESLKKVRNALKTNDWVSTTSEFDNMTKLITNGKTTRIIEKXGF
PPSFIKALFIIQTSHRDL---

---vphprh*qcs*kthsyrsrgllkdfplhlfhflr*yppq*igrsfpftkessslyrr
qnde*srnlclngtlyrinhfpc*tn*tpiscssll*ifs*fcrtk*tylrc*iwyllqk
eg***sltnrwwstswsptswsstprpsspqstttttssttttnhstsspsppksksr*p
tisk*ktsq*qstkisxke

Frame B:
llaywxie**siis*xi*flfiliisn*a*qlfiillfklfphqshhiin*nykrkkrrn
stff*htkphththyillnnvtfl*txfiiriiirviirfrcsskknqlvmfhhlkmklk
rkesfyqlkikfgnnlmkv*kklemllkqmigfqllqnlii*qn*lqmvkqlellkkxvs
hhhllkhyllfkqviei*---

---SLIPDIDSVREKLTRIVQEVSLKTFLFTYSTSYDSILLSELADRFHLPKSQVHSIVA
KMMNNHEISASMEHSTESITFRAEQTKLQYLALHYSESLVDFVEQNERIYDVKFGTSYRK
KGDNDHLPIAGGQHHGHQHHGHQHHGHHHHNQQQQQHHQQQQQTTQHHHHHHQNQNQGNQ
QYQNKKHHNSNQQKSHXK

Frame C:
cwptgx*sdnqlyhxkynfylf**yqikhnnfl*yyylnyflinpit**ikitrekkeet
alffstpnhththiiyy*imsrfyrxgsssesssesssdsdvqvkktnslcfii*r*n*r
eknrfis*r*nlati**kfkks*kcs*nk*lgfnyfri**ydkinykw*nn*ny*krxfp
tiiy*siiyysnks*rf---

---pssqtltvfvknslvsfkrsp*rlsssliplltivsssvnwpivsiyqrvkftlssp
k**iitkslpqwntlpnqslsvlnklnsnillfitlnl*lilsnkmnvftmlnlvppter
rvimityqslvvnimvtnimvinttaiittinnnnniinnnnkplniitittkikikvtn
nikiknitiainknltxr
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHB165 (SHB165Q) /CSM/SH/SHB1-C/SHB165Q.Seq.d/                       1243   0.0
VHA537 (VHA537Q) /CSM/VH/VHA5-B/VHA537Q.Seq.d/                        787   0.0
VHA481 (VHA481Q) /CSM/VH/VHA4-D/VHA481Q.Seq.d/                        787   0.0
SHL158 (SHL158Q) /CSM/SH/SHL1-C/SHL158Q.Seq.d/                        787   0.0
SHE478 (SHE478Q) /CSM/SH/SHE4-D/SHE478Q.Seq.d/                        787   0.0
CHL666 (CHL666Q) /CSM/CH/CHL6-C/CHL666Q.Seq.d/                        787   0.0
AHO844 (AHO844Q) /CSM/AH/AHO8-B/AHO844Q.Seq.d/                        787   0.0
AHO341 (AHO341Q) /CSM/AH/AHO3-B/AHO341Q.Seq.d/                        787   0.0
AHJ465 (AHJ465Q) /CSM/AH/AHJ4-C/AHJ465Q.Seq.d/                        787   0.0
AHR150 (AHR150Q) /CSM/AH/AHR1-C/AHR150Q.Seq.d/                        783   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

CT021721|CT021721.1 KBrH132K06 genomic clone, KBrH (HindIII) BAC library Brassica rapa subsp. pekinensis.    50   2e-06  2
BX255937|BX255937.13 Zebrafish DNA sequence from clone CH211-191D7 in linkage group 20  Contains the gene for a novel protein similar to flavin containing  monooxygenase 5 (fmo5), the fmo5 gene for flavin containing  monooxygenase 5, the gene for a novel protein similar to flavin  containing monooxygenase family, a novel gene (zgc:73360), a novel  gene and three CpG islands.    40   0.005  2
CT245130|CT245130.1 Sus scrofa genomic clone CH242-270B6, genomic survey sequence.    32   0.006  3
CJ456140|CJ456140.1 Macaca fascicularis mRNA, clone: QflA-21961, 5' end, expressed in brain frontal lobe.    32   0.007  4
DQ029097|DQ029097.1 Haematobia irritans irritans mitochondrion, complete genome.    44   0.008  3
AX348755|AX348755.1 Sequence 213 from Patent WO0202807.            40   0.015  2
AX344362|AX344362.1 Sequence 209 from Patent WO0200926.            40   0.015  2
AX251970|AX251970.1 Sequence 231 from Patent WO0168911.            40   0.015  2
BD460434|BD460434.1 Diagnosis of Diseases Associated with Cell Cycle.    40   0.015  2
BD452356|BD452356.1 Diagnosis of Diseases Associated with Cell Cycle.    40   0.015  2
dna update 2005.12.20
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54X97) RecName: Full=Eukaryotic translation initiation factor ...   251   4e-65
(Q17Q06) RecName: Full=Eukaryotic translation initiation factor ...   103   1e-20
(Q99613) RecName: Full=Eukaryotic translation initiation factor ...   102   3e-20
(Q8R1B4) RecName: Full=Eukaryotic translation initiation factor ...   102   3e-20
BC103728_1(BC103728|pid:none) Rattus norvegicus eukaryotic trans...   102   3e-20
AK055366_1(AK055366|pid:none) Homo sapiens cDNA FLJ30804 fis, cl...   102   3e-20
(Q5RAT8) RecName: Full=Eukaryotic translation initiation factor ...   102   3e-20
AK301233_1(AK301233|pid:none) Homo sapiens cDNA FLJ53378 complet...   102   3e-20
BC000533_1(BC000533|pid:none) Homo sapiens eukaryotic translatio...   102   3e-20
BC080763_1(BC080763|pid:none) Mus musculus eukaryotic translatio...   102   3e-20
protein update 2009. 4.15
PSORT 

 psg: 0.25  gvh: 0.30  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.34  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 36.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal

>> prediction for SHB165 is cyt
5' end seq. ID SHB165F
5' end seq. 
>SHB165F.Seq
ACTGTTGGCCTACTGGNGNATAGAGTGATAATCAATTATATCATAGNAAATATAATTTTT
ATTTATTTTAATAATATCAAATTAAGCATAACAACTTTTTATAATATTATTATTTAAATT
ATTTCCTCATCAATCCCATCACATAATAAATTAAAATTACAAGAGAAAAAAAAGAAGAAA
CAGCACTTTTTTTTAGCACACCAAACCACACACACACACACATTATATACTATTAAATAA
TGTCACGTTTTTATAGACANGGTTCATCATCAGAATCATCATCAGAGTCATCATCAGATT
CAGATGTTCAAGTAAAAAAAACCAACTCGTTATGTTTCATCATCTGAAGATGAAATTGAA
GAGAAAAGAATCGTTTTATCAGCTAAAGATAAAATTTGGCAACAATTTGATGAAAGTTTA
AAAAAAGTTAGAAATGCTCTTAAAACAAATGATTGGGTTTCAACTACTTCAGAATTTGAT
AATATGACAAAATTAATTACAAATGGTAAAACAACTAGAATTATTGAAAAAGANGGTTTC
CCACCATCATTTATTAAAGCATTATTTATTATTCAAACAAGTCATAGAGATTTAANNNNN
NNNNN
Length of 5' end seq. 605
3' end seq. ID SHB165Z
3' end seq. 
>SHB165Z.Seq
NNNNNNNNNNGTCCCTCATCCCAGACATTGACAGTGTTCGTGAAAAACTCACTCGTATCG
TTCAAGAGGTCTCCTTAAAGACTTTCCTCTTCACTTATTCCACTTCTTACGATAGTATCC
TCCTCAGTGAATTGGCCGATCGTTTCCATTTACCAAAGAGTCAAGTTCACTCTATCGTCG
CCAAAATGATGAATAATCACGAAATCTCTGCCTCAATGGAACACTCTACCGAATCAATCA
CTTTCCGTGCTGAACAAACTAAACTCCAATATCTTGCTCTTCATTACTCTGAATCTTTAG
TTGATTTTGTCGAACAAAATGAACGTATTTACGATGTTAAATTTGGTACCTCCTACAGAA
AGAAGGGTGATAATGATCACTTACCAATCGCTGGTGGTCAACATCATGGTCACCAACATC
ATGGTCATCAACACCACGGCCATCATCACCACAATCAACAACAACAACAACATCATCAAC
AACAACAACAAACCACTCAACATCATCACCATCACCACCAAAATCAAAATCAAGGTAACC
AACAATATCAAAATAAAAAACATCACAATAGCAATCAACAAAAATCTCACANAAAGAG
Length of 3' end seq. 598
Connected seq. ID SHB165P
Connected seq. 
>SHB165P.Seq
ACTGTTGGCCTACTGGNGNATAGAGTGATAATCAATTATATCATAGNAAATATAATTTTT
ATTTATTTTAATAATATCAAATTAAGCATAACAACTTTTTATAATATTATTATTTAAATT
ATTTCCTCATCAATCCCATCACATAATAAATTAAAATTACAAGAGAAAAAAAAGAAGAAA
CAGCACTTTTTTTTAGCACACCAAACCACACACACACACACATTATATACTATTAAATAA
TGTCACGTTTTTATAGACANGGTTCATCATCAGAATCATCATCAGAGTCATCATCAGATT
CAGATGTTCAAGTAAAAAAAACCAACTCGTTATGTTTCATCATCTGAAGATGAAATTGAA
GAGAAAAGAATCGTTTTATCAGCTAAAGATAAAATTTGGCAACAATTTGATGAAAGTTTA
AAAAAAGTTAGAAATGCTCTTAAAACAAATGATTGGGTTTCAACTACTTCAGAATTTGAT
AATATGACAAAATTAATTACAAATGGTAAAACAACTAGAATTATTGAAAAAGANGGTTTC
CCACCATCATTTATTAAAGCATTATTTATTATTCAAACAAGTCATAGAGATTTAA-----
-----GTCCCTCATCCCAGACATTGACAGTGTTCGTGAAAAACTCACTCGTATCGTTCAA
GAGGTCTCCTTAAAGACTTTCCTCTTCACTTATTCCACTTCTTACGATAGTATCCTCCTC
AGTGAATTGGCCGATCGTTTCCATTTACCAAAGAGTCAAGTTCACTCTATCGTCGCCAAA
ATGATGAATAATCACGAAATCTCTGCCTCAATGGAACACTCTACCGAATCAATCACTTTC
CGTGCTGAACAAACTAAACTCCAATATCTTGCTCTTCATTACTCTGAATCTTTAGTTGAT
TTTGTCGAACAAAATGAACGTATTTACGATGTTAAATTTGGTACCTCCTACAGAAAGAAG
GGTGATAATGATCACTTACCAATCGCTGGTGGTCAACATCATGGTCACCAACATCATGGT
CATCAACACCACGGCCATCATCACCACAATCAACAACAACAACAACATCATCAACAACAA
CAACAAACCACTCAACATCATCACCATCACCACCAAAATCAAAATCAAGGTAACCAACAA
TATCAAAATAAAAAACATCACAATAGCAATCAACAAAAATCTCACANAAAGAG
Length of connected seq. 1183
Full length Seq ID -
Full length Seq. -
Length of full length seq. -