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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHB143
Library SH
(Link to library)
Clone ID SHB143
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID SHB143P
(Link to Original site)
Representative DNA sequence 
>SHB143 (SHB143Q) /CSM/SH/SHB1-B/SHB143Q.Seq.d/
TGTTGGCCTACTGGTTCATCACAAGTATATGATCAACGTATTTTGGCATTAAAAGGAAAA
TGTTATTTAGATTCAAATAAAAATGCATTGTATGATGAAGGTGAACCAGGATTTGGAAAT
GTTGAAATTTCTTTAGGAAATGCAACTGATTATAAGGGAAATAAATATCCAATACGTTAT
TCATCTGCTGATGGTACATTTATATATGATGGATTAACTCAACGTACAACTTATGAATTT
ACATTTACCAATCCAAAAGATTATTACTTTGAAACCTATGAAAAGACATTGGCTTGTCCA
CCAGGTGTACCATGTATTCGTCCAACGGTTTCAGCTGATAATTTGGCAGCAGATCTTTTA
GTTGCAAATACCTTTGCCTATAACATTCCATTGATTAAACAAGTTGGTGTTTCATCAGGC
TCATCTATACTTACAGTTAGAATTAGTGTTGATTTGGGTTATGTTGAGCAAACTCAACCA
CCAACTCAACCACCTACTCAACCACCTACTCAACCACCAACACCTCCACCATTTACTGGT
AGTGCCGTCGCTGGTTTATTATTTACCGATATCAATGGTAATGGAATTTTTGATGGAAGX
XXXXXXXXXGAGTGNAGGTAAATGTGGTAGTCTTGTTGGTGCCGGAGTTGATCAAAAATC
ACTCACTGGTCTTAGATATGGTCCAGGTAATGGTAATTTCTTTGATAATCATAAAACAGG
TCTTCATGACTATACCAATGCTTTTGGTGCAACTAAAGGTGGTCGTGATATTATTGTTAC
AACTGGTTTCGATTACTATGAAGCATTTGAAGGTTCAATTCGTTGGTATAATGCAACCTC
TGGTAAACAACTAAAAGGTTACTCTTTATATATAACCTCTTCAAATGGTCGTACCCCATC
TCCAACCTTTGGTAAACAAAATGGTCTTGGTGATATTACTTCAGTGTATGATTCTTCTCT
TCCACAATATCGTGTTGGTAGAGTTTGGCTTGATATAAATGGTAATGGTATTCAAGATGC
TGGTGAACCTGGTATTTCAGGAATCACAGTATTCTTAATCTATGGTCAACAATCAACTCC
AACAAGTCAAACTGTATCCGATTCAAATGGTTATTTCAAATTTGAAGTTGATTTCAGTTC
AAATTATTATTGTGTAATTCCTGTTGCATTCTTCCAAGCTGGTTCTTCCATTACAATGAG
AAATGCTGATCCAACTCATCCAAATACAAATAGTGATGCTTTCTTATCAACTGTTGATAA
TGTTTATGTCACTTCTTTTACTTCTTCTGATGCTGGGGTTATATTTTAAGGTGTTCATTG
GATATTCCAAAAAAATAAAAAAAAAAAAATA
sequence update 2002.10.25
Translated Amino Acid sequence 
CWPTGSSQVYDQRILALKGKCYLDSNKNALYDEGEPGFGNVEISLGNATDYKGNKYPIRY
SSADGTFIYDGLTQRTTYEFTFTNPKDYYFETYEKTLACPPGVPCIRPTVSADNLAADLL
VANTFAYNIPLIKQVGVSSGSSILTVRISVDLGYVEQTQPPTQPPTQPPTQPPTPPPFTG
SAVAGLLFTDINGNGIFDG---

---SXGKCGSLVGAGVDQKSLTGLRYGPGNGNFFDNHKTGLHDYTNAFGATKGGRDIIVT
TGFDYYEAFEGSIRWYNATSGKQLKGYSLYITSSNGRTPSPTFGKQNGLGDITSVYDSSL
PQYRVGRVWLDINGNGIQDAGEPGISGITVFLIYGQQSTPTSQTVSDSNGYFKFEVDFSS
NYYCVIPVAFFQAGSSITMRNADPTHPNTNSDAFLSTVDNVYVTSFTSSDAGVIF*gvhw
ifqknkkkki
Translated Amino Acid sequence (All Frames) 
Frame A:
CWPTGSSQVYDQRILALKGKCYLDSNKNALYDEGEPGFGNVEISLGNATDYKGNKYPIRY
SSADGTFIYDGLTQRTTYEFTFTNPKDYYFETYEKTLACPPGVPCIRPTVSADNLAADLL
VANTFAYNIPLIKQVGVSSGSSILTVRISVDLGYVEQTQPPTQPPTQPPTQPPTPPPFTG
SAVAGLLFTDINGNGIFDG---

---exr*mw*scwcrs*skithws*iwsr*w*fl**s*nrss*lyqcfwcn*rws*yycy
nwfrll*si*rfnslv*cnlw*ttkrllfiynlfkwsypisnlw*tkwsw*yyfsv*ffs
stiscw*sla*ykw*wysrcw*twyfrnhsilnlwstinsnksncirfkwlfqi*s*fqf
klllcnsccilpswffhynekc*snsskyk**cflinc**clchffyff*cwgyilrcsl
dipkk*kkkn

Frame B:
vgllvhhkyminvfwh*kenvi*iqikmhcmmkvnqdlemlkfl*emqliireiniqyvi
hllmvhlymmd*lnvqlmnlhlpiqkiitlkpmkrhwlvhqvyhvfvqrfqliiwqqif*
lqiplpitfh*lnklvfhqahlylqlelvliwvmlsklnhqlnhllnhllnhqhlhhllv
vpslvyylpismvmeflme---

---SXGKCGSLVGAGVDQKSLTGLRYGPGNGNFFDNHKTGLHDYTNAFGATKGGRDIIVT
TGFDYYEAFEGSIRWYNATSGKQLKGYSLYITSSNGRTPSPTFGKQNGLGDITSVYDSSL
PQYRVGRVWLDINGNGIQDAGEPGISGITVFLIYGQQSTPTSQTVSDSNGYFKFEVDFSS
NYYCVIPVAFFQAGSSITMRNADPTHPNTNSDAFLSTVDNVYVTSFTSSDAGVIF*gvhw
ifqknkkkki

Frame C:
laywfitsi*styfgikrkmlfrfk*kciv**r*triwkc*nffrkcn*l*gk*isntlf
ic*wyiyi*winstynl*iyiyqskrlll*nl*kdiglstrctmyssngfs**fgsrsfs
ckylcl*hsid*tswcfirliytys*n*c*fglc*ansttnsttysttysttntstiyw*
crrwfiiyryqw*wnf*wk---

---vxvnvvvllvpeliknhslvldmvqvmvisliiikqvfmtipmllvqlkvvvilllq
lvsitmkhlkvqfvgimqplvnn*kvtlyi*plqmvvphlqplvnkmvlvillqcmillf
hnivlvefgli*mvmvfkmlvnlvfqesqys*smvnnqlqqvklypiqmvisnlklisvq
iiiv*fllhssklvlplq*emliqliqiqivmlsyqllimfmsllllllmlglyfkvfig
yskkikkkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SHB143 (SHB143Q) /CSM/SH/SHB1-B/SHB143Q.Seq.d/                       2567   0.0
SHB323 (SHB323Q) /CSM/SH/SHB3-A/SHB323Q.Seq.d/                       1417   0.0
SHB816 (SHB816Q) /CSM/SH/SHB8-A/SHB816Q.Seq.d/                       1415   0.0
AHI723 (AHI723Q) /CSM/AH/AHI7-A/AHI723Q.Seq.d/                       1415   0.0
SHB169 (SHB169Q) /CSM/SH/SHB1-C/SHB169Q.Seq.d/                       1413   0.0
SHB113 (SHB113Q) /CSM/SH/SHB1-A/SHB113Q.Seq.d/                       1413   0.0
CHN518 (CHN518Q) /CSM/CH/CHN5-A/CHN518Q.Seq.d/                       1413   0.0
SHB407 (SHB407Q) /CSM/SH/SHB4-A/SHB407Q.Seq.d/                       1411   0.0
SHB488 (SHB488Q) /CSM/SH/SHB4-D/SHB488Q.Seq.d/                       1409   0.0
SHB317 (SHB317Q) /CSM/SH/SHB3-A/SHB317Q.Seq.d/                       1409   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF066073|AF066073.1 Dictyostelium discoideum SP85 (pspB) gene, partial cds.    62   3e-05  1
AF066071|AF066071.1 Dictyostelium discoideum SP85 (pspB) gene, complete cds.    62   3e-05  1
AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.    62   3e-05  1
CK808839|CK808839.1 Rasgsc1686 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara americana cDNA, mRNA sequence.    34   4e-05  3
CK808739|CK808739.1 Rasgsc1529 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara americana cDNA, mRNA sequence.    32   5e-04  3
S72639|S72639.1 PspB=prespore-specific gene {5' region} [Dictyostelium discoideum, Genomic, 1918 nt].    58   5e-04  1
AF066072|AF066072.1 Dictyostelium discoideum SP85 (pspB) gene, partial cds.    58   5e-04  1
AC116963|AC116963.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.    58   5e-04  1
CK808037|CK808037.1 Rasgsc0316 Salivary Gland 4th instar 3rd period (spinning cocoon) Rhynchosciara americana cDNA, mRNA sequence.    34   0.002  3
DT775356|DT775356.1 124719991 TH1 Tribolium castaneum cDNA clone 3C7 3', mRNA sequence.    54   0.008  1
dna update 2005.12.19
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP000686_3542(CP000686|pid:none) Roseiflexus sp. RS-1, complete ...    78   8e-13
CP000909_404(CP000909|pid:none) Chloroflexus aurantiacus J-10-fl...    63   2e-08
CP000910_1528(CP000910|pid:none) Renibacterium salmoninarum ATCC...    61   1e-07
CP001337_3037(CP001337|pid:none) Chloroflexus aggregans DSM 9485...    59   4e-07
(Q7A780) RecName: Full=Serine-aspartate repeat-containing protei...    53   3e-05
AM076203_1(AM076203|pid:none) Staphylococcus aureus partial sdrD...    53   3e-05
AM076200_1(AM076200|pid:none) Staphylococcus aureus partial sdrD...    53   3e-05
AM076196_1(AM076196|pid:none) Staphylococcus aureus partial sdrD...    53   3e-05
AM076198_1(AM076198|pid:none) Staphylococcus aureus partial sdrD...    53   3e-05
CP000703_575(CP000703|pid:none) Staphylococcus aureus subsp. aur...    53   3e-05
protein update 2009. 4.15
PSORT 

 psg: 0.75  gvh: 0.28  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.20  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 40.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for SHB143 is cyt
5' end seq. ID SHB143F
5' end seq. 
>SHB143F.Seq
TGTTGGCCTACTGGTTCATCACAAGTATATGATCAACGTATTTTGGCATTAAAAGGAAAA
TGTTATTTAGATTCAAATAAAAATGCATTGTATGATGAAGGTGAACCAGGATTTGGAAAT
GTTGAAATTTCTTTAGGAAATGCAACTGATTATAAGGGAAATAAATATCCAATACGTTAT
TCATCTGCTGATGGTACATTTATATATGATGGATTAACTCAACGTACAACTTATGAATTT
ACATTTACCAATCCAAAAGATTATTACTTTGAAACCTATGAAAAGACATTGGCTTGTCCA
CCAGGTGTACCATGTATTCGTCCAACGGTTTCAGCTGATAATTTGGCAGCAGATCTTTTA
GTTGCAAATACCTTTGCCTATAACATTCCATTGATTAAACAAGTTGGTGTTTCATCAGGC
TCATCTATACTTACAGTTAGAATTAGTGTTGATTTGGGTTATGTTGAGCAAACTCAACCA
CCAACTCAACCACCTACTCAACCACCTACTCAACCACCAACACCTCCACCATTTACTGGT
AGTGCCGTCGCTGGTTTATTATTTACCGATATCAATGGTAATGGAATTTTTGATGGAAGN
NNNNNNNNN
Length of 5' end seq. 609
3' end seq. ID SHB143Z
3' end seq. 
>SHB143Z.Seq
NNNNNNNNNNGAGTGNAGGTAAATGTGGTAGTCTTGTTGGTGCCGGAGTTGATCAAAAAT
CACTCACTGGTCTTAGATATGGTCCAGGTAATGGTAATTTCTTTGATAATCATAAAACAG
GTCTTCATGACTATACCAATGCTTTTGGTGCAACTAAAGGTGGTCGTGATATTATTGTTA
CAACTGGTTTCGATTACTATGAAGCATTTGAAGGTTCAATTCGTTGGTATAATGCAACCT
CTGGTAAACAACTAAAAGGTTACTCTTTATATATAACCTCTTCAAATGGTCGTACCCCAT
CTCCAACCTTTGGTAAACAAAATGGTCTTGGTGATATTACTTCAGTGTATGATTCTTCTC
TTCCACAATATCGTGTTGGTAGAGTTTGGCTTGATATAAATGGTAATGGTATTCAAGATG
CTGGTGAACCTGGTATTTCAGGAATCACAGTATTCTTAATCTATGGTCAACAATCAACTC
CAACAAGTCAAACTGTATCCGATTCAAATGGTTATTTCAAATTTGAAGTTGATTTCAGTT
CAAATTATTATTGTGTAATTCCTGTTGCATTCTTCCAAGCTGGTTCTTCCATTACAATGA
GAAATGCTGATCCAACTCATCCAAATACAAATAGTGATGCTTTCTTATCAACTGTTGATA
ATGTTTATGTCACTTCTTTTACTTCTTCTGATGCTGGGGTTATATTTTAAGGTGTTCATT
GGATATTCCAAAAAAATAAAAAAAAAAAAATA
Length of 3' end seq. 752
Connected seq. ID SHB143P
Connected seq. 
>SHB143P.Seq
TGTTGGCCTACTGGTTCATCACAAGTATATGATCAACGTATTTTGGCATTAAAAGGAAAA
TGTTATTTAGATTCAAATAAAAATGCATTGTATGATGAAGGTGAACCAGGATTTGGAAAT
GTTGAAATTTCTTTAGGAAATGCAACTGATTATAAGGGAAATAAATATCCAATACGTTAT
TCATCTGCTGATGGTACATTTATATATGATGGATTAACTCAACGTACAACTTATGAATTT
ACATTTACCAATCCAAAAGATTATTACTTTGAAACCTATGAAAAGACATTGGCTTGTCCA
CCAGGTGTACCATGTATTCGTCCAACGGTTTCAGCTGATAATTTGGCAGCAGATCTTTTA
GTTGCAAATACCTTTGCCTATAACATTCCATTGATTAAACAAGTTGGTGTTTCATCAGGC
TCATCTATACTTACAGTTAGAATTAGTGTTGATTTGGGTTATGTTGAGCAAACTCAACCA
CCAACTCAACCACCTACTCAACCACCTACTCAACCACCAACACCTCCACCATTTACTGGT
AGTGCCGTCGCTGGTTTATTATTTACCGATATCAATGGTAATGGAATTTTTGATGGAAG-
---------GAGTGNAGGTAAATGTGGTAGTCTTGTTGGTGCCGGAGTTGATCAAAAATC
ACTCACTGGTCTTAGATATGGTCCAGGTAATGGTAATTTCTTTGATAATCATAAAACAGG
TCTTCATGACTATACCAATGCTTTTGGTGCAACTAAAGGTGGTCGTGATATTATTGTTAC
AACTGGTTTCGATTACTATGAAGCATTTGAAGGTTCAATTCGTTGGTATAATGCAACCTC
TGGTAAACAACTAAAAGGTTACTCTTTATATATAACCTCTTCAAATGGTCGTACCCCATC
TCCAACCTTTGGTAAACAAAATGGTCTTGGTGATATTACTTCAGTGTATGATTCTTCTCT
TCCACAATATCGTGTTGGTAGAGTTTGGCTTGATATAAATGGTAATGGTATTCAAGATGC
TGGTGAACCTGGTATTTCAGGAATCACAGTATTCTTAATCTATGGTCAACAATCAACTCC
AACAAGTCAAACTGTATCCGATTCAAATGGTTATTTCAAATTTGAAGTTGATTTCAGTTC
AAATTATTATTGTGTAATTCCTGTTGCATTCTTCCAAGCTGGTTCTTCCATTACAATGAG
AAATGCTGATCCAACTCATCCAAATACAAATAGTGATGCTTTCTTATCAACTGTTGATAA
TGTTTATGTCACTTCTTTTACTTCTTCTGATGCTGGGGTTATATTTTAAGGTGTTCATTG
GATATTCCAAAAAAATAAAAAAAAAAAAATA
Length of connected seq. 1341
Full length Seq ID -
Full length Seq. -
Length of full length seq. -