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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SHB102
Library SH
(Link to library)
Clone ID SHB102
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16433-1
Original site URL
Representative seq. ID SHB102P
(Link to Original site)
Representative DNA sequence 
>SHB102 (SHB102Q) /CSM/SH/SHB1-A/SHB102Q.Seq.d/
CTGTTGGCCTACTGGNACCATTTTTATTTATTTATTTATTAAATTATTATTTATTTATTA
AATTATTATTTATTGAGTTGTGTAAACTACCTTCCAATAAATTTTAATATTTTGTATATA
TATTCTTGTATAAATAAAAAAAAAAXXXXXXXXXXTGGTTTCCCAAAGACTGTTACCGTT
AAACCAGCCCCATCCGCTGAACACTCTTATGCTGAAGGTGAAGGTTTAGTCAAAGTATTT
GATAATGCCCCAGCTGAATTCACTATTTTCGCCGTTGACACTAAAGGTGTTGCTCGTACC
GATGGTGGTGATCCATTTGAAGTCGCTATCAATGGTCCAGATGGTTTAGTCGTTGATGCC
AAAGTTACCGATAACAATGACGGTACTTATGGTGTTGTCTATGATGCCCCAGTTGAAGGT
AACTACAATGTTAATGTCACCCTCCGTGGTAATCCAATCAAAAATATGCCAATCGATGTC
AAATGCATTGAAGGTGCCAATGGTGAAGATTCATCATTCGGTTCATTCACTTTTACCGTC
GCTGCTAAAAATAAGAAAGGTGAAGTTAAAACCTATGGTGGTGATAAATTTGAAGTCTCT
ATCACTGGTCCAGCTGAAGAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
llaywxhfylfiy*IIIYLLNYYLLSCVNYLPINFNILYIYSCINKKK---

---GFPKTVTVKPAPSAEHSYAEGEGLVKVFDNAPAEFTIFAVDTKGVARTDGGDPFEVA
INGPDGLVVDAKVTDNNDGTYGVVYDAPVEGNYNVNVTLRGNPIKNMPIDVKCIEGANGE
DSSFGSFTFTVAAKNKKGEVKTYGGDKFEVSITGPAEE
Translated Amino Acid sequence (All Frames) 
Frame A:
llaywxhfylfiy*IIIYLLNYYLLSCVNYLPINFNILYIYSCINKKK---

---wfpkdcyr*tspir*tllc*r*rfsqsi**cps*ihyfrr*h*rccsyrww*si*sr
yqwsrwfsr*cqsyr*q*rylwccl*cps*r*lqc*chppw*snqkyanrcqmh*rcqw*
rfiirfihfyrrc*k*er*s*nlww**i*slyhwss*r

Frame B:
cwptgtifiylfikllfiy*iiiy*vv*ttfq*ilifciyilv*ikkk---

---GFPKTVTVKPAPSAEHSYAEGEGLVKVFDNAPAEFTIFAVDTKGVARTDGGDPFEVA
INGPDGLVVDAKVTDNNDGTYGVVYDAPVEGNYNVNVTLRGNPIKNMPIDVKCIEGANGE
DSSFGSFTFTVAAKNKKGEVKTYGGDKFEVSITGPAEE

Frame C:
vgllxpflfiyllnyylfikllfielcklpsnkf*yfvyiflyk*kk---

---vsqrllplnqphplntlmlkvkv*skylimpqlnslfspltlkvllvpmvvihlksl
smvqmv*slmpklpitmtvlmvlsmmpqlkvttmlmspsvviqskicqsmsnalkvpmvk
ihhsvhsllpsllkirkvklkpmvvinlkslslvqlkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHQ144 (VHQ144Q) /CSM/VH/VHQ1-B/VHQ144Q.Seq.d/                        926   0.0
VHI544 (VHI544Q) /CSM/VH/VHI5-B/VHI544Q.Seq.d/                        926   0.0
VHC802 (VHC802Q) /CSM/VH/VHC8-A/VHC802Q.Seq.d/                        926   0.0
VHC710 (VHC710Q) /CSM/VH/VHC7-A/VHC710Q.Seq.d/                        926   0.0
VHB620 (VHB620Q) /CSM/VH/VHB6-A/VHB620Q.Seq.d/                        926   0.0
VFK681 (VFK681Q) /CSM/VF/VFK6-D/VFK681Q.Seq.d/                        926   0.0
SLH892 (SLH892Q) /CSM/SL/SLH8-D/SLH892Q.Seq.d/                        926   0.0
SHK351 (SHK351Q) /CSM/SH/SHK3-C/SHK351Q.Seq.d/                        926   0.0
SHJ823 (SHJ823Q) /CSM/SH/SHJ8-A/SHJ823Q.Seq.d/                        926   0.0
SHG675 (SHG675Q) /CSM/SH/SHG6-D/SHG675Q.Seq.d/                        926   0.0
own update 2004. 5.12
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X15430|X15430.1 Dictyostelium discoideum mRNA for gelation factor.   926   0.0    2
AQ543434|AQ543434.1 RPCI-11-349G2.TV RPCI-11 Homo sapiens genomic clone RPCI-11-349G2, genomic survey sequence.    48   0.22   1
AL356308|AL356308.16 Human DNA sequence from clone RP11-475L16 on chromosome 10.    48   0.22   1
CR940353|CR940353.1 Theileria annulata genomic DNA chromosome 4.    36   0.40   2
AC134000|AC134000.2 Rattus norvegicus clone CH230-463P7, *** SEQUENCING IN PROGRESS ***, 4 unordered pieces.    42   1.3    2
AC123148|AC123148.5 Rattus norvegicus clone CH230-43D20, WORKING DRAFT SEQUENCE, 2 unordered pieces.    34   1.6    2
AC119537|AC119537.5 Rattus norvegicus clone CH230-426J14, *** SEQUENCING IN PROGRESS ***, 4 unordered pieces.    34   1.7    2
DV482383|DV482383.1 5380_H09_O18Z_065 Brachypodium distachyon leaf EST library Brachypodium distachyon cDNA clone 5380_H09_O18, mRNA sequence.    44   3.4    1
BF983023|BF983023.1 602308354F1 NIH_MGC_88 Homo sapiens cDNA clone IMAGE:4399645 5', mRNA sequence.    44   3.4    1
CB142294|CB142294.1 K-EST0196054 L11SNU354s1 Homo sapiens cDNA clone L11SNU354s1-21-F05 5', mRNA sequence.    44   3.4    1
dna update 2005.12.19
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF080395_1(AF080395|pid:none) Entamoeba histolytica actin bindin...    85   2e-15
U70560_1(U70560|pid:none) Entamoeba histolytica actin binding pr...    75   2e-12
(Q1PRL4) RecName: Full=Tripartite motif-containing protein 71; A...    65   2e-09
AF220021_1(AF220021|pid:none) Homo sapiens tripartite motif prot...    64   3e-09
AF045239_1(AF045239|pid:none) Homo sapiens brain expressed ring ...    64   3e-09
AF220022_1(AF220022|pid:none) Homo sapiens tripartite motif prot...    64   3e-09
(O75382) RecName: Full=Tripartite motif-containing protein 3; Al...    62   1e-08
BC151315_1(BC151315|pid:none) Bos taurus tripartite motif-contai...    62   1e-08
(Q9R1R2) RecName: Full=Tripartite motif-containing protein 3; Al...    62   2e-08
AK170029_1(AK170029|pid:none) Mus musculus NOD-derived CD11c +ve...    62   2e-08
protein update 2009. 4.15
PSORT 

 psg: 0.89  gvh: 0.39  alm: 0.36  top: 0.80  tms: 0.00  mit: 0.31  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: mitochondrial
	 28.0 %: cytoplasmic
	 28.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane
	  4.0 %: endoplasmic reticulum

>> prediction for SHB102 is mit
5' end seq. ID SHB102F
5' end seq. 
>SHB102F.Seq
CTGTTGGCCTACTGGNACCATTTTTATTTATTTATTTATTAAATTATTATTTATTTATTA
AATTATTATTTATTGAGTTGTGTAAACTACCTTCCAATAAATTTTAATATTTTGTATATA
TATTCTTGTATAAATAAAAAAAAAANNNNNNNNNN
Length of 5' end seq. 155
3' end seq. ID SHB102Z
3' end seq. 
>SHB102Z.Seq
NNNNNNNNNNTGGTTTCCCAAAGACTGTTACCGTTAAACCAGCCCCATCCGCTGAACACT
CTTATGCTGAAGGTGAAGGTTTAGTCAAAGTATTTGATAATGCCCCAGCTGAATTCACTA
TTTTCGCCGTTGACACTAAAGGTGTTGCTCGTACCGATGGTGGTGATCCATTTGAAGTCG
CTATCAATGGTCCAGATGGTTTAGTCGTTGATGCCAAAGTTACCGATAACAATGACGGTA
CTTATGGTGTTGTCTATGATGCCCCAGTTGAAGGTAACTACAATGTTAATGTCACCCTCC
GTGGTAATCCAATCAAAAATATGCCAATCGATGTCAAATGCATTGAAGGTGCCAATGGTG
AAGATTCATCATTCGGTTCATTCACTTTTACCGTCGCTGCTAAAAATAAGAAAGGTGAAG
TTAAAACCTATGGTGGTGATAAATTTGAAGTCTCTATCACTGGTCCAGCTGAAGAAA
Length of 3' end seq. 477
Connected seq. ID SHB102P
Connected seq. 
>SHB102P.Seq
CTGTTGGCCTACTGGNACCATTTTTATTTATTTATTTATTAAATTATTATTTATTTATTA
AATTATTATTTATTGAGTTGTGTAAACTACCTTCCAATAAATTTTAATATTTTGTATATA
TATTCTTGTATAAATAAAAAAAAAA----------TGGTTTCCCAAAGACTGTTACCGTT
AAACCAGCCCCATCCGCTGAACACTCTTATGCTGAAGGTGAAGGTTTAGTCAAAGTATTT
GATAATGCCCCAGCTGAATTCACTATTTTCGCCGTTGACACTAAAGGTGTTGCTCGTACC
GATGGTGGTGATCCATTTGAAGTCGCTATCAATGGTCCAGATGGTTTAGTCGTTGATGCC
AAAGTTACCGATAACAATGACGGTACTTATGGTGTTGTCTATGATGCCCCAGTTGAAGGT
AACTACAATGTTAATGTCACCCTCCGTGGTAATCCAATCAAAAATATGCCAATCGATGTC
AAATGCATTGAAGGTGCCAATGGTGAAGATTCATCATTCGGTTCATTCACTTTTACCGTC
GCTGCTAAAAATAAGAAAGGTGAAGTTAAAACCTATGGTGGTGATAAATTTGAAGTCTCT
ATCACTGGTCCAGCTGAAGAAA
Length of connected seq. 612
Full length Seq ID -
Full length Seq. -
Length of full length seq. -